Divergent controlling factors of freeze–thaw-induced changes in dissolved organic carbon and microbial biomass carbon between topsoil and subsoil of cold alpine grasslands

Abstract

Freeze-thaw (FT) processes in cold regions significantly influence the mineralization and sequestration of soil organic carbon (SOC), particularly in its active pools, such as dissolved organic carbon (DOC) and microbial biomass carbon (MBC). However, manipulation experiments often amplify the effects of natural FT processes and overlook soils below 20 cm depth. To investigate carbon responses to seasonal FT events across soil depths (0–80 cm) and identify controlling factors, field sampling was conducted before freezing and after thawing in alpine grassland soils on the Qinghai-Tibet Plateau. Results are as follows: 1) After soil thawing, the entire soil profile showed insignificant changes in SOC (−4.46%) and MBC (3.21%), but DOC (45.27%) and DOC/SOC (160.08%) increased significantly, and strong associations were observed in DOC changes between adjacent soil layers. 2) FT indicators (number and temperature amplitude of daily FT cycles, frozen temperature, and frozen days) influenced SOC changes differently in the topsoil (0–10 cm) and subsoil (20–30 cm). Contrary to the impact of frozen temperature, other FT indicators largely facilitated the production of DOC0–10 and DOC20–30. The effects of FT indicators on MBC were nonlinear. 3) Structural equation models indicated that average soil water governed FT-induced changes in both DOC0–10 and DOC20–30. The varying effects of porosity and pH from topsoil to subsoil, along with the weakening relationship between changes in DOC and MBC, favored a stronger increase in DOC20–30. Increases in soil water and DOC after thawing stimulated the increment of MBC0–10, while porosity (positively) and sand content (negatively) regulated the MBC20–30 change. It indicates that, influenced by soil structure and biogeochemical environment, DOC and MBC in grassland soils are more sensitive to natural FT processes than SOC. Nevertheless, changes in FT patterns due to long-term climate change may cumulatively affect SOC.