Scientific Experiments Research Articles

A new experimental station for microbeam X-ray scattering at the SSRF BL10U1 beamline

Benefiting from the development of high-brilliance synchrotron radiation sources, microbeam X-ray scattering has become a well established scattering-based imaging technology. This article describes the newly constructed time-resolved microbeam small-angle X-ray scattering (µSAXS) experimental station at the BL10U1 beamline at the Shanghai Synchrotron Radiation Facility. The µSAXS endstation provides SAXS/WAXS measurements with a 10 µm hard X-ray beam and a flux of ∼1012 photons s−1. A multi-axis sample stage, an on-axis viewer and in situ experimental apparatus are incorporated to facilitate multi-method scientific experiments in various material fields. As scientific examples, this article explores 1D tomography, 2D mapping and tomographic sectioning based on X-ray scattering to investigate the micro–nanostructures of polymer fiber, spherulite and bamboo samples.

Ciència recreativa a la natura, una visió sobre el terreny

The aim of this collaboration is to reflect on outdoor recreational science and its relationship with teaching, scientific dissemination, collecting or geological tourism. I propose a radical vision of scientific experimentation outside the academic field, with more varied techniques, processes and materials, emphasising the importance of the «object», often of natural origin, as a motivator of perception, fine observation and scientific curiosity. I also briefly consider the impact it can have on the natural environment and the need to find a balance between human pressure and respect for the environment. Finally, I propose the inclusion of recreational scientific activities in camps, rural hotels, and other accommodation in nature, and even in specific spaces, as a necessary leisure activity for the knowledge society.

Opportunities and limits of lunar gravitational-wave detection.

A new era of lunar exploration has begun with participation of all major space agencies. This activity brings opportunities for revolutionary science experiments and observatories on the Moon. The idea of a lunar gravitational-wave detector wasalready proposed during the Apollo programme. The key characteristic of the Moon is that it is seismically extremely quiet. It was also pointed out that the permanently shadowed regions at the lunar poles provide ideal conditions for gravitational-wave detection. In recent years, three different detector concepts were proposed with varying levels of technological complexity and science potential. In this paper, we confront the three concepts in terms of their observational capabilities based on a first more detailed modelling of instrumental noise. We identify important technological challenges and potential show-stoppers. This article is part of a discussion meeting issue 'Astronomy from the Moon: the next decades (part 2)'.

Abstract 817: Establishing a niche to improve Africa’s contribution to cancer research

Abstract Africa has been a key source of scientific discoveries to the global community, especially in areas of scientific experiments and implementation. However, Africans are underrepresented in their contribution to global science and within the African countries, access to adequate funding and infrastructure to do high quality science is limited. These inequities contribute to the 10/90 gap in which only an estimated 10% of global health research funding is used to address health problems in Africa. Thus, it is not surprising that today, Africa contributes just about 2 percent of world research output, 1.3 percent of research spending, and 0.1 percent of all patents. To bridge the aforementioned gaps, mitigate the effect of brain drain and structural constraints and improve support for the next generation scientists, the Nigerian Government recently established the National Institute for Cancer Research and Treatment (NICRAT). It is worthy to note that cancer incidence and mortality rates in Africa are increasing 1.1 million new cases, with 711,000 deaths reported in 2020. This indicate a mortality-to-incidence ratio (MIR) of 0.64. These cancer challenges in Africa necessitates a concerted approach towards effective cancer control and management. Here, we show in detail how the NICRAT support improving science while integrating with Nigerian national development plans and strategies. We also show how the NICRAT is establishing a mechanism for domestic funding, while fostering national and international collaborations for African scientists and encouraging women into careers in cancer research. Currently, the NICRAT’s key task also includes centralizing cancer control efforts, mobilizing funding from different sources for cancer research, and coordinating cancer treatment activities across Nigeria. In addition, the institute facilitates collaboration between researchers, healthcare providers, and patients, and provides guidance for cancer policy formulation and implementation. The establishment of NICRAT by Nigeria’s government as well as similar centers elsewhere in Africa may improve active participation of African scientists in scientific practice, discourse, and decision making and spotlight their intellectual contributions towards solving science-associated global problems. Citation Format: Usman A. Malami, Sani A. Malami, Uchechukwu E. Nwokwu, Usman W. Mohammed, Faruk Mohammed. Establishing a niche to improve Africa’s contribution to cancer research [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 817.

Enhancing the Skills of Hi Tech Lab Utilization in Teaching Geometrical Concepts

Abstract
 Teaching learning is a wonderful experience in every learning places.Particularly in schools it happened every moment.School labs are a great place for students which help them enhance their learning by understanding the theoretical concepts of mathematics which are taught in classrooms. Well-designed Hi-techlaboratories not only make science experiments fun but also help students in achieving good academic results. The world has been taken over by modern technology all around. Even the education sector has adopted the benefits of scientific advancements to offer quality education programmes to the students and teachers. The aim of this present study reveals that the skills of Hi-tech labutilization in teaching geometrical concepts.

Influence of probiotic drug Propionovy on productive traits and physiological condition of broiler chickens

Recently, the use of various biological feed additives and especially probiotic effects in feeding of farm animals has become relevant. The purpose of the work was to study the effect of the probiotic drug Propionovy on the productive traits and physiological state of broiler chickens. Experimental studies were carried out under the conditions of Kuzbass Broiler LLC b the Kemerovo region. The object of the study was broiler chickens of the cross Hubbard ISA F-15. The studies examined the effect of certain doses of the probiotic drug Propionovy on the productive traits, physiological state of broiler chickens and the choice of the optimal dose of the probiotic. In order to conduct a scientifi c and economic experiment, groups of poultry were formed using the method of analogous groups, while day-old chickens were selected, from which four experimental groups were formed (control and three experimental). Thus, the 1st control group received the main diet adopted on the farm, consisting of complete feed, which included a feed antibiotic. In the 2nd, 3rd and 4th experimental groups, from days 1 to 4, chickens received the same basic diet as chickens in the control group. Starting from the 5th day, instead of a feed antibiotic, the probiotic drug Propionovy was added to the diets of chickens of the 2nd, 3rd and 4th experimental groups in doses of: 0,35; 0,50 and 0,65 ml/head, respectively, the doses were then increased according to the research methodology. The results received on base of the experiment the maximum revenue from product sales was 40 562,73 rubles was obtained in the 4th experimental group, which was 4,11 % more than in the 1st control group. The greatest economic effi ciency was also in the 4th experimental group and amounted to 547,26 rubles from experimental livestock or 2,76 rubles in terms of 1 head. Thus, economic calculations carried out based on the results of scientific and economic experiment indicate a positive eff ect of feeding the probiotic drug Propionovy on the prime-cost of production.

Learning Outcomes of the Inquiry-in-Action Model for Chemistry Experimentation

There is overwhelming evidence supporting the decline of science education in Liberia. In an effort to contribute to the solution, we conducted a study to examine the impact of inquiry-based experimentation and conventional demonstration on the conceptual understanding of solutions and solubility among grade eleven students. We also investigated the relationship between students' achievement scores in solutions and solubility and their science inquiry process skills. A cluster random sample of eight schools was initially selected from 12 out of 31 high schools that had the necessary space and materials for experimentation. Using a Non-equivalent control group research design, the experimental group received instruction using inquiry-based experimentation, while the control group received instruction using traditional demonstration methods, for a period of six weeks. The results were analyzed using the Mann-Whitney U, and Wilcoxon Signed Rank tests. The findings indicated that inquiry-based experimentation reduced students' anxiety and increased their confidence in chemistry experiments. Although the inquiry-in-action model enhanced learners' conceptual understanding of solutions and solubility, it did not significantly improve their mastery of the mathematical components of the test. However, it did significantly enhance students' science inquiry process skills. It should be noted that while the inquiry-in-action model positively influenced learners' attitudes toward their preferences, behavior, and anxieties in chemistry experiments, it also led to a general demotivation to learn chemistry through experimentation. Additionally, there was a positive correlation between students' attitudes toward teaching and learning chemistry through experiments and their science inquiry process skills. However, this correlation was stronger between students' attitudes towards the demonstration of experiments and their science inquiry process skills compared to the attitudes towards the inquiry-in-action model and science inquiry process skills. This suggests that the traditional demonstration method is not entirely ineffective, and a combination of both demonstration and inquiry-based experimentation approaches may be a viable way to maximize the benefits of science experiments.

Toward HPC application portability via C++ PSTL: the Gaia AVU-GSR code assessment

The computing capacity needed to process the data generated in modern scientific experiments is approaching ExaFLOPs. Currently, achieving such performances is only feasible through GPU-accelerated supercomputers. Different languages were developed to program GPUs at different levels of abstraction. Typically, the more abstract the languages, the more portable they are across different GPUs. However, the less abstract and co-designed with the hardware, the more room for code optimization and, eventually, the more performance. In the HPC context, portability and performance are a fairly traditional dichotomy. The current C++ Parallel Standard Template Library (PSTL) has the potential to go beyond this dichotomy. In this work, we analyze the main performance benefits and limitations of PSTL using as a use-case the Gaia Astrometric Verification Unit-Global Sphere Reconstruction parallel solver developed by the European Space Agency Gaia mission. The code aims to find the astrometric parameters of ∼108\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\sim10^8$$\\end{document} stars in the Milky Way by iteratively solving a linear system of equations with the LSQR algorithm, originally GPU-ported with the CUDA language. We show that the performance obtained with the PSTL version, which is intrinsically more portable than CUDA, is comparable to the CUDA one on NVIDIA GPU architecture.

A domestic-like carrot cooking methodology for multiple research applications

Domestic-oriented research focusing on food requires methodologies that closely mimic practices occurring in home kitchens while meeting scientific standards. Currently however, there is a lack of methodologies that can be implemented in both laboratory and home environments. This paper proposes a method that fulfills the scientific requirements of repeatability and reproducibility, while utilizing commonly available materials and processes found in the average household. The method is applied to the preparation, boiling, and seasoning of roots of Daucus carota L. (“carrots”), which can be employed in various scientific fields with only minor adjustments. Three scientific experiments utilizing this methodology are presented, namely sensory evaluation, ionic chromatography measurements, and NMR experiments. In the existing literature, numerous protocols have been used for carrot sample preparation, hindering direct comparisons between studies. In this paper we would like to highlight the ability of the methodology to enhance comparability, as well as its potential utilization in other research applications. The main principles underlying the proposed methodology can also be extrapolated to prepare samples of several other vegetables or cereals.•Comprehensive guidelines for standardizing the shapes, lengths, and widths of carrots are outlined, ensuring minimal variability while preserving the integrity of the raw material.•The cooking method for carrots is tailored to utilize commonly available household materials, while meeting scientific standards required for research purposes.•Seasoning practices involving readily available domestic materials, like commercial salt, are suggested.

Scientific Knowledge in Bacon Philosophy: Insights from Dialectical Materialism

Francis Bacon occupies a central position in the history of the philosophy of science, representing a pivotal shift from medieval to early modern scientific thought. Therefore, in the contemporary context, understanding Bacon's philosophy is crucial for appreciating the foundations of modern science and its ongoing evolution. The purpose of the article is to examine the enduring relevance of Francis Bacon's philosophy of science, particularly in the context of his emphasis on empirical methodology, experimentation, and the socio-cultural dimensions of scientific knowledge. By analyzing Bacon's contributions, the study aims to elucidate his methodological innovations and their implications for contemporary scientific practice and theory. The work uses thematic and comparative analysis to determine the role of F. Bacon's philosophy in the analysis of the concept of knowledge.The research highlights Bacon's seminal contributions to the philosophy of science, emphasizing his advocacy for empirical methodology, systematic experimentation, and the socio-cultural context of scientific inquiry. By critiquing the methodological limitations of scholastic philosophy and advocating for a methodological overhaul grounded in experience and observation, Bacon laid the groundwork for modern experimental science. In the conclusion, his integration of dialectical materialism and interdisciplinary perspectives underscores the significance of collective scientific endeavor and the foundational role of natural history in establishing the ontological basis of scientific knowledge. By elucidating Bacon's vision for a science that serves the general welfare of society, the study underscores the continued importance of his insights for addressing contemporary challenges and advancing scientific knowledge for the betterment of humanity.

Effects of first vs. third-person perspective and self- versus other-avatars on user movements in virtual reality

Social science experiments in virtual reality (VR) frequently manipulate the perspective from which a user views an avatar and the identity of an avatar to change participant perceptions and attitudes. However, avatar embodiment may also influence physical behaviors—i.e., the way participants move—during the VR experience. For example, users may shift their position to align with avatars they see from a first-person perspective, a phenomenon known as the self-follower effect. We conducted a between-subjects, pre-registered study to understand how common techniques such as manipulating perspective and appearance might affect participant movements while in VR. We demonstrate that even when participants do not have agency over their avatars' movements, viewing avatar movements influences their own actions, whether these are viewed from the first or the third-person perspective. These phenomena hold potential as interventions to prompt participant movements in other contexts.

Integration of Digital Twins Technologies in Education for Experiential learning: Benefits and Challenges

In order to provide an immersive and engaging learning environment, digital twins in education are virtual representations of real-world items, settings, or people. From elementary to secondary school to higher education establishments, digital twins can be employed in a variety of educational contexts. For instance, students can imitate scientific experiments that would be hard to execute in the classroom owing to safety issues or a lack of resources, by using digital twins to create virtual versions of the investigations. With the help of digital twins, educators may even plan virtual field trips that let learners travel the globe without ever leaving the classroom. For instance, students might be able to study history, tour of Taj Mahal in Agra in-depth, and even engage with historical personalities through a digital duplicate of the wall. Students can communicate with lecturers or subject matter experts virtually by using digital duplicates to build virtual versions of them. In remote learning environments, where students do not have access to actual classrooms or in-person instructors, this can be especially helpful. This research study discusses the advantages of using digital twin technologies extensively in the educational system, as well as the technological, social, and ethical constraints.

Optimal 3D angular sampling with applications to cryo-EM problems

The goal of cryo-EM experiments in the biological sciences is to determine the atomic structure of a molecule and deduce insights into its functions and mechanisms. Despite improvements in instrumentation for data collection and new software algorithms, in most cases, individual atoms are not resolved. Model building of proteins, nucleic acids, or molecules in general, is feasible from the experimentally determined density maps at resolutions up to the range of 3–4 Angstroms. For lower-resolution maps or parts of maps, fitting smaller structures obtained by modelling or experimental techniques with higher resolution is a way to resolve the issue. In practice, we have an atomic structure, generate its density map at a given resolution, and translate/rotate the map within a region of interest in the experimental map, computing a measure-of-fit score with the corresponding areas of the experimental map. This procedure is computationally intensive since we work in 6D space. An optimal ordered list of rotations will reduce the angular error and help to find the best-fitting positions faster for a coarse global search or a local refinement. It can be used for adaptive approaches to stop fitting algorithms earlier once the desired accuracy has been achieved. We demonstrate how the performance of some fitting algorithms can be improved by grouping sets of rotations. We present an approach to generate more efficient 3D angular sampling, and provide the computer code to generate lists of optimal orientations for single and grouped rotations and the lists themselves.

Final physical design of engineering materials diffractometer at the China spallation neutron source

Neutron diffraction is a widely utilized technique in detecting microstructures and residual strains in large-scale engineering components. In this study, we present the final physical design of an upcoming neutron diffractometer for engineering materials at the China Spallation Neutron Source. Our primary objective is to minimize the experimental time required for precise diffraction peak determination. To achieve this, the design includes a neutron transport system, a neutron wavelength selection system, a diffracted neutron optical system, and a resolution and intensity regulation system. Notably, we optimized the wavelength bandwidth specifically for scientific experiments, while the neutron flux or integrated peak intensity were optimized through careful considerations of neutron transport. Additionally, we placed great emphasis on ensuring the uniformity of the neutron phase space to enhance measurement accuracy. Moreover, we optimized the combinations of resolution and integrated peak intensity based on the best figure of merit. Consequently, we successfully developed an advanced diffractometer that is poised to offer new capabilities for engineering material studies.

Inferred wind speed and direction during the descent and landing of Perseverance on Mars

Perseverance successfully parachuted into Jezero crater close to its western rim on a Martian spring afternoon. In situ observations of the wind conditions during the parachute and powered descent are unavailable. These observations are important for characterising the Planetary Boundary Layer (PBL) and to better constrain atmospheric conditions in Jezero crater.We infer the winds during Perseverance's parachute descent from an altitude of 12 km down to 2 km by fitting a trajectory model to the reconstructed trajectory data published by the M2020 Entry, Descent and Landing team. Below 2 km altitude we infer the winds by analysing a High Resolution Imaging Science Experiment (HiRISE) image of the landing site that was obtained about one sol after the landing.Our inferred wind speed and direction profile indicate high speed winds at an altitude of 7 km blowing from the east and at 3 km blowing from the southwest. Below an altitude of 2 km, the winds are inferred to be easterlies. The heat shield impact on the surface was observed by Perseverance's Lander Vision System Camera (LCAM). Afterwards, ejecta clouds were observed moving towards the north of the impact site. These observations, together with the orientation of the parachute canopy on the surface, suggest near-surface winds at the time were southerlies.We have inferred the wind speed and direction during Perseverance's descent that are consistent with its horizontal motion as revealed by trajectory data and HiRISE image. Comparisons with atmospheric models suggest the high speed winds could be related to topographic forcing. This study provides constraints for atmospheric modelling and advances the characterisation of winds in Jezero crater, focusing on the vertical structure of the complex wind field present in the crater.

Research on Tracking and Positioning Technology for Structural Components of Assembled Buildings

This article critically examines Charles Bonnet’s seminal contributions to experimental science, particularly through his influential work, ‘Research on the Use of Leaves.’ The primary objective is to delineate the functions and forms of natural experimentation as they were understood and practiced in the mid-18th century. During this period, scientists esteemed experimentation as the zenith of scientific proof, acutely cognizant of its multifaceted functional and typological aspects. Their methodology was characterized by a detailed narration of research methodologies, fostering a ‘rhetoric of transparency’ in scientific discourse. Nevertheless, the advent of new norms in scientific discourse during the 19th century, coupled with a paradigmatic philosophical shift in the 20th century, resulted in the marginalization of empirical practices of the Enlightenment era. Contemporary historians are now endeavoring to reconcile significant terminological discrepancies and to recontextualize the divergent methodologies among natural historians of that epoch. This involves a critical distinction between exploratory and demonstrative (or discriminant) forms of experimentation. Bonnet’s systematic preference for discriminant experimentation serves as a quintessential example of the logical approach in natural history. However, this perspective does not wholly represent the 18th-century ‘art of observation,’ which embraced a plethora of methodologies including taxonomy, anatomical dissection, chemical analysis, and physical measurements.

Regression Analysis of the Relationship Between ESG Performance and Firms’ Technological Innovation Capability

This article critically examines Charles Bonnet’s seminal contributions to experimental science, particularly through his influential work, ‘Research on the Use of Leaves.’ The primary objective is to delineate the functions and forms of natural experimentation as they were understood and practiced in the mid-18th century. During this period, scientists esteemed experimentation as the zenith of scientific proof, acutely cognizant of its multifaceted functional and typological aspects. Their methodology was characterized by a detailed narration of research methodologies, fostering a ‘rhetoric of transparency’ in scientific discourse. Nevertheless, the advent of new norms in scientific discourse during the 19th century, coupled with a paradigmatic philosophical shift in the 20th century, resulted in the marginalization of empirical practices of the Enlightenment era. Contemporary historians are now endeavoring to reconcile significant terminological discrepancies and to recontextualize the divergent methodologies among natural historians of that epoch. This involves a critical distinction between exploratory and demonstrative (or discriminant) forms of experimentation. Bonnet’s systematic preference for discriminant experimentation serves as a quintessential example of the logical approach in natural history. However, this perspective does not wholly represent the 18th-century ‘art of observation,’ which embraced a plethora of methodologies including taxonomy, anatomical dissection, chemical analysis, and physical measurements.

The Relationship Between a Green, Low-carbon and Recycling Economy, Economic Policy and Industrial Structure Development

This article critically examines Charles Bonnet’s seminal contributions to experimental science, particularly through his influential work, ‘Research on the Use of Leaves.’ The primary objective is to delineate the functions and forms of natural experimentation as they were understood and practiced in the mid-18th century. During this period, scientists esteemed experimentation as the zenith of scientific proof, acutely cognizant of its multifaceted functional and typological aspects. Their methodology was characterized by a detailed narration of research methodologies, fostering a ‘rhetoric of transparency’ in scientific discourse. Nevertheless, the advent of new norms in scientific discourse during the 19th century, coupled with a paradigmatic philosophical shift in the 20th century, resulted in the marginalization of empirical practices of the Enlightenment era. Contemporary historians are now endeavoring to reconcile significant terminological discrepancies and to recontextualize the divergent methodologies among natural historians of that epoch. This involves a critical distinction between exploratory and demonstrative (or discriminant) forms of experimentation. Bonnet’s systematic preference for discriminant experimentation serves as a quintessential example of the logical approach in natural history. However, this perspective does not wholly represent the 18th-century ‘art of observation,’ which embraced a plethora of methodologies including taxonomy, anatomical dissection, chemical analysis, and physical measurements.

Искусственный интеллект против ученого в вопросе о сохранении баланса между правом на информацию и необходимостью защиты персональных данных от незаконного использования

This article reflects the results of a scientific experiment caused by a discussion about the possibility of replacing scientists with neural networks. The development of digital technologies today raises many controversial issues in science and legal practice. One of the most popular is the question of how to ensure human protection in the context of the transformation of inviolable rights and freedoms: information, privacy and inviolability. The large-scale spread of new technologies poses many questions to the regulator, one of which is the question of finding a balance between the right to information and the need to ensure the protection of personal data in a dynamic and constantly evolving Internet environment. Such a choice is faced by the legislator due to the fact that the spread of innovations involves constant work with personal data, accelerating their exchange, storage and protection. The introduction of digital technologies is beneficial for the development of public services, interaction and control, which ultimately leads to increased efficiency and accelerated information exchange for citizens and legal entities. Taking into account the progressive development of artificial intelligence technologies, it was quite interesting to conduct an experiment comparing the answers to the question of maintaining a balance between the right to information and the need to protect personal data given by scientists and artificial intelligence, in particular, the YandexGPT2 neural network. The responses of the neural network are analyzed for compliance with the main criteria of scientific work: reliability, objectivity and novelty, and are evaluated in comparison with the response of the authors of the scientific article.

Optimization and Performance Analysis of CAT Method for DNA Sequence Similarity Searching and Alignment.

Bioinformatics is a rapidly developing field enabling scientific experiments via computer models and simulations. In recent years, there has been an extraordinary growth in biological databases. Therefore, it is extremely important to propose effective methods and algorithms for the fast and accurate processing of biological data. Sequence comparisons are the best way to investigate and understand the biological functions and evolutionary relationships between genes on the basis of the alignment of two or more DNA sequences in order to maximize the identity level and degree of similarity. This paper presents a new version of the pairwise DNA sequences alignment algorithm, based on a new method called CAT, where a dependency with a previous match and the closest neighbor are taken into consideration to increase the uniqueness of the CAT profile and to reduce possible collisions, i.e., two or more sequence with the same CAT profiles. This makes the proposed algorithm suitable for finding the exact match of a concrete DNA sequence in a large set of DNA data faster. In order to enable the usage of the profiles as sequence metadata, CAT profiles are generated once prior to data uploading to the database. The proposed algorithm consists of two main stages: CAT profile calculation depending on the chosen benchmark sequences and sequence comparison by using the calculated CAT profiles. Improvements in the generation of the CAT profiles are detailed and described in this paper. Block schemes, pseudo code tables, and figures were updated according to the proposed new version and experimental results. Experiments were carried out using the new version of the CAT method for DNA sequence alignment and different datasets. New experimental results regarding collisions, speed, and efficiency of the suggested new implementation are presented. Experiments related to the performance comparison with Needleman-Wunsch were re-executed with the new version of the algorithm to confirm that we have the same performance. A performance analysis of the proposed algorithm based on the CAT method against the Knuth-Morris-Pratt algorithm, which has a complexity of O(n) and is widely used for biological data searching, was performed. The impact of prior matching dependencies on uniqueness for generated CAT profiles is investigated. The experimental results from sequence alignment demonstrate that the proposed CAT method-based algorithm exhibits minimal deviation, which can be deemed negligible if such deviation is considered permissible in favor of enhanced performance. It should be noted that the performance of the CAT algorithm in terms of execution time remains stable, unaffected by the length of the analyzed sequences. Hence, the primary benefit of the suggested approach lies in its rapid processing capabilities in large-scale sequence alignment, a task that traditional exact algorithms would require significantly more time to perform.