Abstract Background: Ascites can create a progressive microenvironment for tumor cells, whereas there is few evidence on the role of ascites on tumor response in ovarian cancer. Thus, we investigated cellular response by ascites in ovarian cancer cell line, and evaluated the effect of ascites on clinical outcomes in patients with advanced-stage ovarian cancer. Methods: Moreover, we collected clinico-pathologic database of 277 patients with stage IIIC-IV ovarian cancer, and investigated factors affecting overall response (OR) and survival. Furthermore, we collected ascites from four patients with stage IIIC-IV serous adenocarcinoma of the ovary, and cell migration, invasiveness and proliferation by adding ascites were evaluated in SKOV-3. Results: OR rates were not different between patients with ascites and those without ascites (77.8% vs. 89.1%; P = 0.094), and no ascites was associated with better progression-free survival with marginal significance (37.4 vs. 27.1 mons; P = 0.056) in spite of no effect of ascites on overall survival. In multivariate analyses, no ascites was a favorable factor for reducing the risk of non-OR with marginal significance (adjusted OR, 0.286; 95% CI, 0.080-1.018; P = 0.053), whereas ascites was not an independent factor affecting survival. Moreover, ascites increased migration and invasiveness of SKOV-3 (Figs. 1A and 1B). Although proliferation of SKOV-3 was decreased for the first 24 h, it increased in time-dependent manner till 72h (Fig. 1C). Conclusions: Ascites may contribute to invasion, migration and proliferation of ovarian cancer cells. Our clinical findings also support that ascites may affect overall response after primary treatment, suggesting the possibility that ascites can act as a tumor microenvironment related with tumor cell survival. Citation Format: Soochi Kim, Anna Kim, Hee Seung Kim, HyeRan Gwak, UnTek Jo, Yong Sang Song. Effect of ascites on tumor response in advanced stage ovarian cancer. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 1536. doi:10.1158/1538-7445.AM2015-1536
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