Abstract. Amid global warming, the timely supplementation of soil water is crucial for the effective restoration and protection of the ecosystem. It is therefore of great importance to understand the temporal and spatial variations of soil water sources. The research collected 2451 samples of soil water, precipitation, river water, ground ice, supra-permafrost water, and glacier snow meltwater in June, August, and September 2020. The goal was to quantify the contribution of various water sources to soil water in the Three-Rivers Headwater Region (China) during different ablation periods. The findings revealed that precipitation, ground ice, and snow meltwater constituted approximately 72 %, 20 %, and 8 % of soil water during the early ablation period. The snow is fully liquefied during the latter part of the ablation period, with precipitation contributing approximately 90 % and 94 % of soil water, respectively. These recharges also varied markedly with altitude and vegetation type. The study identified several influencing factors on soil water sources, including temperature, precipitation, vegetation, evapotranspiration, and the freeze–thaw cycle. However, soil water loss will further exacerbate vegetation degradation and pose a significant threat to the ecological security of the “Chinese Water Tower”. It emphasizes the importance of monitoring soil water, addressing vegetation degradation related to soil water loss, and determining reasonable soil and water conservation and vegetation restoration models.