Salivary Antioxidant Research Articles

Analyzing the Salivary Antioxidant Capacity in Children before and after Dental Caries Restoration: A Comparative Scientific Investigation

Aim: The purpose of this study is to examine the total antioxidant capacity (TAC) as a biomarker to investigate oxidative stress in different pathological conditions. The primary goal is to compare the TAC levels in saliva before and after the restoration of carious teeth. Additionally, the study aims to explore potential differences in TAC between genders with the intention of establishing a possible link between salivary TAC and dental caries. Materials and Methods: In this research, we employed a random selection process to assemble two cohorts: Group I, comprising 27 male individuals, and Group II, encompassing 27 females, all falling within the age bracket of 7 to 10 years. Saliva samples were procured from each participant both thirty days prior to and subsequent to the restorative dental intervention. To gauge the levels of total antioxidant capacity (TAC), we employed Cayman’s antioxidant assay kit and quantified the outcomes employing a nanodrop instrument. Results: The obtained data was subjected to statistical analysis using SPSS version 18.0. The paired t-test was used to compare the TAC levels before and after the restorative procedure within each group (Group I and Group II). The results revealed that after restoration, both Group I and Group II showed a significant increase in TAC levels compared to before restoration (P = 0.000). Additionally, the Student t-test was employed to compare the TAC levels between Group I (males) and Group II (females). Interestingly, the study observed that the TAC of saliva was significantly higher in males compared to females, both before and after the restoration of carious teeth. Conclusion: Our research demonstrated reduced TAC levels in areas affected by dental caries, and these levels exhibited a significant rise following the restoration of all carious teeth. Consequently, it can be deduced that the evaluation of caries activity and the effectiveness of its treatment can be facilitated through the assessment of salivary factors, which holds promise for applications in preventive dentistry. Clinical Significance: TAC can be employed as a biomarker and a therapeutic target because a reduced TAC level before restoration is a sign of infection.

Effects of brisk walking with or without music on body composition, standing balance, cardiovascular parameters, and salivary biomarkers in older women.

This study aimed to assess and compare changes in body composition, standing balance, cardiovascular parameters, and salivary biomarkers, particularly salivary antioxidant status, after brisk walking training with or without music in older women. Twenty-four subjects were randomly assigned to brisk walking groups: with music (BWM) (n=12) or without music (BW) (n=12). Eighteen subjects completed the exercise training (9 in each group), and their data were used for analysis. The research protocols were classified into three phases: pretraining phase, training phase, and posttraining phase, while the data collection was divided into four sessions: resting condition, during treadmill exercise testing, immediately posttreadmill exercise testing, and 5-min posttreadmill exercise testing defined as after the cool-down session. The results showed that 8 weeks of home-based brisk walking with or without music did not improve standing balance, blood pressure, salivary biomarkers including total protein concentration, and antioxidant status but maintained or prevented the decline of these parameters. Only the BWM group reduced fat mass relative to increasing fat-free mass (P<0.05) and improved recovery heart rate (P<0.05) by modifying cardiac autonomic control in posttreadmill exercise testing. Therefore, brisk walking with preferred music can be a tool to delay the progression of cardiovascular dysfunction in older women. A longer duration of the exercise program and larger groups of participants are needed for further investigation of brisk walking with or without music on physiological and biochemical changes.

Artificial saliva plus beta-glucan for treatment of xerostomia in older adults: The clinical study of effectiveness and salivary total antioxidant capacity levels

To evaluate the effectiveness of 2 artificial saliva formulations, sodium carboxymethylcellulose artificial saliva spray and sodium carboxymethylcellulose artificial saliva spray plus β-glucan.: This study was a double-blind randomized controlled trial and involved older adults with xerostomia. The xerostomia inventory score, clinical oral dryness score, oral moisture degree, unstimulated salivary flow rate, and salivary total antioxidant capacity were measured prior to and after treatment. The results were evaluated 4 weeks after taking both products. : The study included 51 subjects in the control (n = 25) and BG (n = 26) groups. xerostomia inventory score and clinical oral dryness score were significantly lower in both groups after 4 weeks of treatment (p&amp;#60;0.00). Additionally, both groups had significantly greater oral moisture degree and unstimulated salivary flow rate than before treatment (p&amp;#60;0.05). Only the increase in oral moisture degree in the BG group was significantly greater than that in the control group (p=0.048). Both groups tended to have decreased salivary total antioxidant capacity, but only the control group had a significant difference at 4 weeks (p=0.004). There was no significant difference in satisfaction score between the two groups. No serious side effects were found in the study. : The clinical signs and symptoms of xerostomia were improved by both control and artificial BG saliva. BG improved oral moisture more than the control. Furthermore, BG was more likely to prevent a decrease in salivary total antioxidant capacity than was the control.

Salivary levels of catalase, total antioxidant capacity and interleukin-1β and oral health-related quality of life after matcha and green tea consumption for patients with gingivitis: A randomized clinical trial.

To evaluate the changes in salivary levels of catalase (CAT), total antioxidant capacity (TAC) and IL-1β (primary outcomes) as well as gingival health and oral health-related quality of life (OHRQOL) 1 month after the consumption of matcha and green tea in adult patients with gingivitis. This study was a parallel randomized clinical trial. Forty gingivitis adult patients were randomly assigned to drink either matcha or green tea cup twice daily for a duration of 1 month. They were asked to keep their habitual oral hygiene measures. Plaque index (PI) and bleeding on probing (BOP) were recorded. In addition, saliva was collected at baseline and 1 month after tea consumption. Salivary levels of CAT, TAC and IL-1β were determined by ELISA. Each participant was asked to fill OHIP-14 questionnaire before and after tea consumption. Following tea intake, 40.6% of the study sample had healthy gingiva. Both groups similarly showed reductions in PI (0.32 ± 0.11-0.22 ± 0.08) and BOP (0.24 ± 0.08-0.13 ± 0.07) scores after tea consumption. There was a significant difference between mean TAC before and after matcha tea consumption only. Non-significant reductions in salivary levels of CAT and IL-1β were detected after tea consumption. OHRQOL of participants was improved after tea, both types, consumption. Despite study limitations, daily consumption of matcha and green tea might be beneficial as they potentially enhance OHRQOL of individuals with gingivitis and reduce the extent of gingivitis despite non-significant changes in salivary antioxidants and IL-1β levels.

Review of salivary antioxidants and their barriers

Background: Oxidative stress results from the imbalance between reactive oxygen species (ROS) and cellular antioxidant defenses. Oxidative stress can cause damage to cellular structures such as cell membranes, lipids, proteins, lipoproteins, and deoxyribonucleic acids when free radicals are present in excess. Antioxidant mechanisms protect tissues against oxidative stress by decreasing free radical levels in cells through the inhibition of activities or expression of free radical-generating enzymes. Purpose: The purpose of this paper is to discuss the role of antioxidants in protecting cells from the harmful effects of ROS, particularly in the context of oral mucosa, and to clarify which molecules disrupt the pathway of these antioxidants. Review: Salivary peroxidase, catalase, superoxide dismutase, and glutathione reductase are enzymatic salivary antioxidants, and uric acid, reduced glutathione, albumin, and lactoferrin are non-enzymatic antioxidants. Both types of antioxidants regularly donate an electron to free radicals to reduce their levels in cells. Conclusion: Antioxidant activities may be disrupted by signaling mechanisms resulting from barrier regulations observed in tissue. The increased expression of malondialdehyde is an indicator of disrupted antioxidant barriers.

Ramadan Fasting in Relation to Salivary Uric Acid and Selective Physical Parameters

Background: Ramadan is the holy month in which all Muslims abstain from eating and drinking from sunrise to sunset. The impact of Ramadan fasting on the oral cavity and saliva needs more attention because of the important function of saliva in the protection of the mouth. Objective: To determine the impact of Ramadan fasting on salivary uric acid, flow rate, and pH among Iraqi male dental students. Patients and Methods: The subject population consisted of thirty healthy male dental students (College of Dentistry/University of Baghdad) with an age range of 20–23 years who intended to fast the whole of Ramadan (1443 Hijri calendar). Saliva samples were obtained from all dental students one week prior to the start of Ramadan, the last week of Ramadan, and one month after the end of Ramadan. The salivary flow rate was calculated, and the salivary pH was measured using a digital pH meter. Then a saliva sample was analyzed to detect the concentration of uric acid by using the spectrophotometer. Results: The data analysis for this study revealed that the salivary flow rate decreased from baseline to Ramadan and then increased again after one month of Ramadan with significant differences (p&lt;0.05). While salivary uric acid increased continuously with significant differences from baseline to Ramadan and after one month of Ramadan fasting (p&lt;0.05), on the other hand, salivary pH showed no significant differences (p &gt; 0.05) among the three time periods of the study. Conclusion: Saliva was affected by Ramadan fasting through a decrease in salivary flow rate during fasting and a return to normal salivary flow rate after the end of Ramadan. In addition, uric acid, which acts as a salivary antioxidant, shows an increase during Ramadan fasting.

Prevalence of dental caries among coronavirus disease 2019-recovered patients and correlation with salivary total antioxidant capacity in Kalaburagi region of Indian subpopulation.

Coronavirus disease 2019 (COVID-19) has vastly affected mankind worldwide, and its long-term effect on oral health needs to be studied. The aim of the study was to evaluate the prevalence of dental caries and its correlation with salivary total antioxidant capacity (TAC) in Kalaburagi region of the Indian subpopulation. About 80 subjects (16-55 years) were included in the study based on selection criteria and were divided into two groups (n = 40). Group 1: no history of COVID-19 infection and Group 2: COVID-19-recovered subjects. Each group was subdivided into two subgroups (n = 20) A and B with and without dental caries. Dental caries was assessed using the decay, missing, filled, treatment (DMFT) index by the World Health Organization. About 5 ml of unstimulated saliva samples was collected and centrifuged at 2000 rpm for 10 min, and the supernatants were collected. Salivary TAC was estimated using 2,2'-azino-bis-[3-ethylbenzothiazoline-6-sulfonic] acid assay and observed under spectrophotometer (734 nm). Data were analyzed using the Shapiro-Wilk test for normality distribution followed by one-way analysis of variance and post hoc Tukey test (P < 0.05). COVID-19-recovered subjects showed a higher prevalence of dental caries and lower TAC levels than non-COVID-19 subjects. Higher prevalence of dental caries with lower levels of salivary TAC was found in COVID-19-recovered individuals.

Treatment regimens and disease activity could alter salivary myeloperoxidase levels in patients with inflammatory bowel diseases.

Inflammatory bowel diseases (IBDs) present with alternating periods of exacerbation and remission; therefore, it is necessary to develop noninvasive diagnostic tools to control the disease activity and improve therapeutic effectiveness. Recently, we have found that patients with ulcerative colitis (UC) who qualified for biologic therapy had significantly lower salivary myeloperoxidase (MPO) levels. This cross‑sectional study aimed to assess the impact of IBD activity and applied treatment on salivary antioxidant system as reflected by the levels of catalase, total antioxidant status, and MPO. The study group comprised 99 patients diagnosed with Crohn disease (CD) and 61 patients with UC. The Crohn Disease Activity Index and modified Mayo scale were used to estimate the clinical activity of CD and UC, respectively. Unstimulated whole mixed saliva was collected. Salivary levels of selected markers were measured with enzyme‑linked immunosorbent assays and colorimetric assays. The patients with clinically active UC showed significantly decreased median (interquartile range) salivary MPO levels (79.4 [30.1-157.5] vs 94.8 [58.2-274.7] ng/ml) with significant correlations with the endoscopic stage on the Mayo scale (R = 0.423; P = 0.02). Receiver operating characteristic analysis confirmed a potential usefulness of MPO concentrations in predicting clinically active UC (area under the curve = 0.654; P = 0.03; cutoff <210.4 ng/ml). Moreover, in the patients treated with biologics and without steroid therapy, salivary MPO concentrations negatively correlated with neutrophil counts in the individuals with UC and positively with C‑reactive protein level in the patients with CD. Salivary MPO levels changed depending on the disease activity in the patients with UC. Decreased MPO concentration in the saliva could be a predictor of clinically active UC.

Comparative Evaluation and Correlation of Salivary Total Antioxidant Capacity with Dental Caries among Smokers and Tobacco Chewers in North Karnataka Region of Indian Sub Population - A Clinico Biochemical Study

Background Tobacco chewing and smoking influence salivary antioxidant levels and therefore their effect on dental caries incidence needs to be evaluated. Objectives To correlate the effect of smoking and tobacco chewing on salivary pH and total antioxidant capacity TAC in individuals with and without dental caries in North Karnataka region of India.Methods About 180 male patients aged 20-60 years were chosen and allotted to three groups n60. Group 1 Non-smokers and chewers. Group 2 Only tobacco chewers and Group 3 Only smokers. Each group was further split into two subgroups n30 A with dental caries B without dental caries. Dental caries was assessed by WHO-recommended DMFT Decayed Missing Filled Teeth criteria and salivary samples were obtained by spitting method. Salivary pH was determined and supernatants from centrifuged samples were subjected to ferric reducing antioxidant power FRAP assay for estimating TAC levels. The one-way analysis of variance ANOVA and Post Hoc Tukey tests were used for statistical analysis P lt0.05.Results Higher caries rate with lower pH and decreased salivary TAC levels were found in tobacco chewers and smokers with and without dental caries compared to non-tobacco chewers and non-smokers with significant difference.Conclusion Tobacco chewers and smokers showed decreased salivary TAC levels with lower pH and high caries rate compared to individuals without these habits.

A study to evaluate the role of antioxidants and iron as diagnostic markers in oral cancer

Oral cavity (OC) and oropharyngeal (OP) cancers afflict about 500,000 people globally. Dental health care practitioners need to understand the causes, diagnosis, and treatment of oral cavities and oropharyngeal cancer. The constant and direct exposure of oral mucosal cells to tobacco product chemical carcinogens such as polynuclear aromatic hydrocarbons and nitrosamines causes the generation of free radicals/reactive oxygen species. Changes in salivary antioxidant and micronutrient levels may prove to be an important factor in the pathogenesis of oral premalignant and malignant disorders. The present study aimed to evaluate the levels of Vitamin C, MDA and iron levels in oral cancer, pre-malignant group and the oral cancer group. 15 oral cancer patients, 30 premalignant condition patients, and 15 normal controls were analyzed in the study. Among the 30 patients of the premalignant conditions- group (Group S1) 12 were diagnosed with oral submucous fibrosis, and 18 of them were diagnosed with oral leukoplakia. The oral cancer patients (Group S2) were segregated based on the TNM system from stages I to IV. The oral submucous fibrosis patients were subdivided into four grades. The oral leukoplakia patients were categorized into homogenous and speckled types. A detailed history was obtained from the patients. After obtaining informed consent, saliva samples will be collected from patients who have not consumed any food or any form of tobacco 2 hours before collection. Following a thorough mouth rinse using distilled water, saliva is allowed to accumulate in his or her mouth for 5 minutes. Accumulated saliva is collected by the spit method. 5ml of blood sample will also be collected from these patients, Serum was separated by Centrifugation. Collected serum and saliva were stored at -80℃ until further analysis. The serum and salivary samples were then analyzed for Vitamin C, iron, and malonaldehyde (lipid peroxidation) according to the standardized protocol. In the present study, a total of 60 samples were screened for Vitamin C, Iron, and MDA in both serum and saliva of normal control, premalignant, and oral cancer patients. We found that there was a decreased level of Vitamin C and iron in both serum and saliva of premalignant and oral cancer patients. MDA level was higher in premalignant, and oral cancer patients and there was a significant increase in the level of MDA in oral cancer patients. Understanding antioxidants can help reduce the incidence of oral cancer in its early stages. More study is needed before this supplement may be recommended as an adjuvant treatment.

Comparative Evaluation of Efficacy of Chlorhexidine with Centella asiatica in the Management of Stage 2 Grade B Periodontitis in Type 2 Diabetes Mellitus Patients − A Randomised Controlled Clinical Trial

Introduction: Chemical plaque control agents are used as an adjunct to non-surgical periodontal therapy. Adjunctives can assist in proper oral hygiene maintenance. Type 2 diabetes mellitus is a metabolic disease that can reflect in the periodontium. Periodontal disease prevention and management in Type 2 diabetes patients need routine dental care. Over counter mouthwashes used by patients can result in side effects; this has popularised the use of herbal mouthrinses that have minimal side effects. Aim: The present study aimed at evaluating the efficacy of chlorhexidine (CHX) with Centella asiatica in the management of Stage 2 Grade B periodontitis in type 2 diabetes mellitus patients. Materials and method: Seventy Type 2 diabetes mellitus patients with periodontitis were recruited for this study. In Group A, 35 subjects received scaling and root planing (SRP) and 10% C. asiatica mouthwash. In Group B, 35 subjects received SRP and 0.2% CHX mouthwash (Colgate-plax, Colgate-Palmolive). Clinical periodontal outcomes measured were plaque index (PI), gingival index (GI), bleeding on probing (BOP) and probing depth (PD) at baseline, 1 month and 3 months post therapy. Salivary total antioxidant capacity (TAOC) and Interleukin 1β levels were also measured at baseline and 3 months. Results: Clinical parameters PI, GI, BOP and PD were reduced significantly in groups A and B, post-treatment, 3-month follow-up. However, the intergroup comparison didnot show any significant difference. Salivary TAOC levels were significantly increased in both groups after 3 months therapy. The intergroup comparison of salivary TAOC levels between groups didnot show any significant difference. Salivary interleukin 1β levels were significantly reduced in both groups post-treatment. Conclusion: This study suggests the anti-inflammatory and antioxidant potential of C. asiatica mouthwash and a safe alternative to CHX mouthwash as an adjunct in the management of Stage 2 Grade B periodontitis in type 2 diabetes mellitus patients.

Salivary antioxidents in relation to caries severity among a group of young adults

Background: Saliva represents the body's stages of toxicological, hormonal, immunological, and markers of many infectious disease and disorders, so that it's an ideal tool for monitoring oral and systemic health.&#x0D; Antioxidants help the body's defensive system by reducing oxidative stress.&#x0D; &#x0D; Aims of the study: The study was directed to estimate the carious lesion experience (DMFS) in relation to salivary superoxide dismutase and total protein among adults by age and gender.&#x0D; &#x0D; Materials and methods: Caries experiences was assessed according to DMFS index, after investigation eighty peoples of an age 19-22 years for both genders. Stimulated salivary samples were together and chemically assessed to detect selected salivary antioxidants (superoxide dismutase, total protein), under standardized condition. Total protein and superoxide dismutase were measured by spectrophotometric examination.&#x0D; &#x0D; Results: With not-significant differences, total protein was found to be higher in the caries active group than in the caries free group, but salivary superoxide dismutase was the opposite. with statistically not-significant differences, females had a greater average of the salivary total protein than men, whereas men had a greater average of salivary superoxide dismutase than females. the first group (19-20 years) had a not-significant greater average salivary total protein and salivary superoxide dismutase than the second group (21-22 years).&#x0D; Conclusion: Salivary superoxide dismutase is the important factor in dental caries prevention. As a result, alterations in salivary content are crucial in the development and progression of dental caries.

Total Antioxidant Capacity of Saliva and Its Correlation with pH Levels among Dental Students under Different Stressful Conditions.

(1) Background: This cross-sectional study conducted at the Faculty of Dental Medicine, Timisoara, Romania, between December 2022 and February 2023 aims to assess salivary total antioxidant capacity and pH levels in dental students experiencing non-stressful and stressful situations and explore potential correlations between these factors. (2) Methods: Saliva samples were collected during two different periods: before an Oral Health course and before the Oral Health exam, under stressful conditions. Ethical principles were followed, and informed consent was obtained. Data on age, gender, health status, drug use, smoking habits, and anxiety levels were recorded. Saliva was collected using the draining method and pH was measured using indicator paper strips. Total antioxidant capacity (TAC) was determined using a commercial assay kit. Statistical analysis involved descriptive statistics, Student's t-test to compare pH and TAC between study groups, and Pearson's correlation coefficient to analyze the correlation between salivary pH and TAC within each group, with p < 0.05 indicating significance. (3) Results: This study involved 80 participants, comprising 26 males and 54 females, all enrolled in the 5th year of the Oral Health course, with ages ranging from 20 to 53 and a mean age of 23.62 (±4.19) years. Pearson's correlation results show a statistically significant negative relationship between the STAI test and TAC during the stress-free period (-0.02 **, N = 80, p < 0.01). (4) Conclusions: There are variations in saliva's antioxidant capacity in response to different stress conditions. Dental students experienced a higher level of stress before academic assessments compared to the non-stress period during the course.

Salivary Antioxidants Role in Oral Health and Diseases (A Review Article)

Background: Saliva is the first defense system of the body through its antioxidant status (total antioxidant capacity), which is either enzymatic or non-enzymatic antioxidants, which block the process of oxidation by neutralizing free radicals; therefore, the objective of this review is to evaluate the role of salivary antioxidant biomarkers in the diagnosis and monitoring of many oral and periodontal diseases and related systemic disorders. Method: We searched for articles published on PubMed and Google Scholar in the last 20 years and then filtered the mints 60 papers according to the following keywords: saliva, total antioxidant capacity (TAC) and oxidative stress (OS). Conclusion: Saliva samples can be beneficial in demonstrating oxidant-antioxidant balance in oral and systemic illnesses and may be a suitable sample type in the diagnosis and monitoring of many pathologies in the body.

Comparison of Total Salivary Antioxidant Capacity of Children with and without Severe Early Childhood Caries before and after Complete Dental Rehabilitation.

Early childhood caries (ECC) is the most chronic disease affecting children all over the world. Children often complain of pain and need complete rehabilitation, which is often done under general anesthesia due to extensive treatment and behavioral problems that are often encountered. The present study was undertaken to evaluate the total salivary antioxidant capacity of children with severe ECC (S-ECC) before and after complete dental rehabilitation and compare it with caries-free children. Salivary samples were obtained from 30 children aged 3-5 years and diagnosed with S-ECC, along with 30 age and sex-matched controls. Complete dental rehabilitation under general anesthesia was performed on the children with S-ECC, and follow-up salivary samples were obtained after 3 and 6 months postoperatively. Total salivary antioxidant capacity was measured using a commercially available Oxygen Radical Absorbance Antioxidant Assay measurement kit. At baseline, children with S-ECC had higher total antioxidant capacity (TAC) compared to the controls. A significant difference in total salivary antioxidant capacity between both groups was seen at baseline. At 3 months following, the total salivary antioxidant capacity of children treated for S-ECC was slightly higher than controls but had significantly reduced compared to baseline. At 6 months, there was no significant difference between both groups. Within the limitations of our study, it can be concluded that the total salivary antioxidant capacity associated with children suffering from S-ECC is reduced after complete rehabilitation. Mathew MG, Jeevanandan G. Comparison of Total Salivary Antioxidant Capacity of Children with and without Severe Early Childhood Caries before and after Complete Dental Rehabilitation. Int J Clin Pediatr Dent 2023;16(3):474-477.

Salivary Oxidative Stress and Antioxidant Capacity in Smokeless Tobacco (Naswar) Users.

Naswar, a smokeless tobacco product, commonly consumed in Pakistan, is associated with a 10-fold increase in the risk of oral cancer. However, little is known about Naswar's underlying toxicity mechanisms. The current study aimed to investigate the effects of Naswar use on oral health and salivary parameters. A case-control study was conducted among Naswar users (n=42) and age-matched healthy controls (n=42) in Pakistan in 2019. Participant data were collected using questionnaires. Decayed, missing, and filled teeth (DMFT) scores were computed during clinical examinations. Unstimulated whole saliva was collected to assess salivary flow rate, pH, and salivary total oxidative stress (TOS)/total antioxidant capacity (TAC) using commercially available kits. Participants' oral health parameters were compared between cases and controls using ANOVA. No significant differences were observed between the two groups in terms of age, oral health, and hygiene practices and mean DMFT score. Mean salivary pH and the salivary flow rate was significantly (p<0.001) higher in Naswar users (7.7 and 0.71 mL/minute, respectively) than in non-users (6.95 and 0.52 mL/minute, respectively). Although TOS and TAC were not significantly different between the groups, Naswar users generally had a higher TOS (51.6±42 µmol/L) and lower TAC (0.55±0.18 mmol/L) than non-users (TOS 45.5±38.2 µmol/L and TAC 0.57±0.17 mmol/L). Correlational analysis also revealed a significant positive correlation between DMFT score andNaswar use duration (r=0.796, p<0.001)and the number of dips/units consumed each day (r=0.515; p<0.001). Habitual Naswar use is associated with increased salivary flow rate, pH, and TOS, and reduced TAC levels in Pakistani adults compared to non-users. The pro-oxidant changes may contribute toward deleterious effects of Naswar use including oral cancer.

Effect of non-surgical periodontal therapy on saliva levels of paraoxonase-1 in healthy and periodontitis subjects: a clinico-biochemical study

Background: Salivary antioxidants play main roles in the defensive mechanism of saliva against free radicals. Prevalence of inflammatory conditions like periodontitis associated with several pathogeneses, knowing the defensive mechanism of salivary antioxidant against free radicals can provide helpful strategies in diagnosis and evaluation of these conditions. The aim of this study was evaluation of the paraoxonase (PON1) levels of saliva in healthy and periodontitis subjects. Methods: In this clinico-biochemical study 30 patients diagnosed with periodontitis and 30 healthy controls aged between 18 to 60 years, 30 patients were recalled after non-surgical periodontal therapy were selected. Three millilitres (3 ml) of whole unstimulated saliva sample were collected. PON-1 level were determined by a commercially available enzyme-linked immunosorbent assay (ELISA) kit. Results: The results showed a statistically significant difference in the salivary PON1 levels in pre (66.6600%) and post (89.3200%) treatment samples (p value &lt;0.001). The saliva level of PON1 was improved compared to post treatment. There was an improvement in the clinical parameters, and seen a statistically significant difference with p=0.001. Conclusions: The reduction in the saliva levels of paraoxonase in periodontitis patients has led to a variation leading to increased ROS and therefore periodontal breakdown. The variation in salivary paraoxonase levels before and after treatment can be taken as an indicator of success of periodontal therapy.

Salivary stimulatory effect of novel low level transcutaneous electro neurostimulator in geriatric patients with xerostomia

BackgroundXerostomia (dryness of the mouth) is one of the most common long-term consequences of ageing, and it causes a tremendous impact on the function and morphology of the salivary ductal system. As a consequence, it leads to a decrease in the amount of salivary output and also affects the overall quality of life. The purpose of this study was to determine whether electrostimulation using a custom designed transcutaneous electrical nerve stimulation (TENS) device will help to improve the quality of secreted saliva following electrostimulation.MethodsOne hundred thirty-five participants underwent the intervention for three months, twice daily (80 Hz). Pre-intervention and post-intervention unstimulated saliva were collected. Parameters such as salivary pH, cortisol level, salivary antioxidants, total protein, the viscosity of saliva, and microbial carriage were analysed.ResultsSalivary pH, cortisol, microbial cultures, viscosity, and antioxidants showed a significant difference at the end of the 3rd month (p < 0.05). Irrespective of the patient's age, gender, and common underlying systemic illnesses (diabetes and hypertension), a significant change in the quality of the salivary analytes was observed.ConclusionThe study emphasises the use of a custom designed TENS device in improving the quality of secreted saliva among old patients with oral dryness.

Analysis of salivary flow rate, biochemical composition, and redox status in orchiectomized spontaneously hypertensive rats

ObjectiveThis study aimed to analyze the salivary flow rate, biochemical composition, and redox status in orchiectomized spontaneously hypertensive rats (SHR) compared to normotensive Wistar rats. DesignThirty-two young adult male SHR and Wistar (3-months-old) rats were randomly distributed into four groups; either castrated bilaterally (ORX) or underwent fictitious surgery (SHAM) as Wistar-SHAM, Wistar-ORX, SHR-SHAM, and SHR-ORX. Two months beyond castration, pilocarpine-induced salivary secretion was collected from 5-month-old rats to analyze salivary flow rate, pH, buffer capacity, total protein, amylase, calcium, phosphate, sodium, potassium, chloride, thiobarbituric acid reactive substances (TBARs), carbonyl protein, nitrite, and total antioxidant capacity. ResultsThe salivary flow rate was higher in the Wistar-ORX compared to the Wistar-SHAM group, while remaining similar between the SHR-SHAM and SHR-ORX groups. ORX did not affect pH and salivary buffer capacity in both strains. However, salivary total protein and amylase were significantly reduced in the Wistar-ORX and SHR-ORX compared to the respective SHAM groups. In both ORX groups, salivary total antioxidant capacity and carbonylated protein were increased, while lipid oxidative damage (TBARs) and nitrite concentration were higher only in the Wistar-ORX than in the Wistar-SHAM group. In the Wistar-ORX and SHR-ORX, the salivary calcium, phosphate, and chloride were increased while no change was detected in the SHAM groups. Only salivary buffering capacity, calcium, and chloride in the SHR-ORX adjusted to values similar to Wistar-SHAM group. ConclusionHypertensive phenotype mitigated the orchiectomy-induced salivary dysfunction, since the disturbances were restricted to alterations in the salivary biochemical composition and redox state.

Salivary Antioxidant Profile in Patients with Systemic Sclerosis and Periodontitis.

The aim of the present study was to compare periodontal status and antioxidant profile in unstimulated saliva of systemic sclerosis (SSc) patients with periodontitis and systemically healthy periodontitis patients. Twenty patients with established diagnoses of systemic sclerosis and periodontitis (SSc group) and 20 systemically healthy individuals with periodontitis (P group) were enrolled in the study. Clinical periodontal parameters (clinical attachment level (CAL), gingival recession (GR), periodontal probing depth (PPD), and gingival index (GI)) and concentration of uric acid (UA), superoxide dismutase (SOD), and glutathione peroxidase (GPX) in unstimulated saliva samples were assessed. There were significantly higher mean values of CAL (4.8 ± 0.21 mm versus 3.18 ± 0.17 mm; p ≤ 0.001) and GR (1.66 ± 0.90 mm versus 0.46 ± 0.54 mm; p ≤ 0.001) in the SSc group when compared to the P group. Significantly higher level of GPX (p ≤ 0.001) and SOD (p ≤ 0.001) in unstimulated saliva was detected in the SSc group in comparison with the P group. The specific activity of UA did not significantly differ between the two groups (p = 0.083). The results may indicate higher periodontal destruction and antioxidant perturbations in unstimulated saliva of SSc patients with periodontitis compared to systemically healthy periodontitis patients.