Effect of non-surgical periodontal therapy on saliva levels of paraoxonase-1 in healthy and periodontitis subjects: a clinico-biochemical study

Abstract

Background: Salivary antioxidants play main roles in the defensive mechanism of saliva against free radicals. Prevalence of inflammatory conditions like periodontitis associated with several pathogeneses, knowing the defensive mechanism of salivary antioxidant against free radicals can provide helpful strategies in diagnosis and evaluation of these conditions. The aim of this study was evaluation of the paraoxonase (PON1) levels of saliva in healthy and periodontitis subjects. Methods: In this clinico-biochemical study 30 patients diagnosed with periodontitis and 30 healthy controls aged between 18 to 60 years, 30 patients were recalled after non-surgical periodontal therapy were selected. Three millilitres (3 ml) of whole unstimulated saliva sample were collected. PON-1 level were determined by a commercially available enzyme-linked immunosorbent assay (ELISA) kit. Results: The results showed a statistically significant difference in the salivary PON1 levels in pre (66.6600%) and post (89.3200%) treatment samples (p value <0.001). The saliva level of PON1 was improved compared to post treatment. There was an improvement in the clinical parameters, and seen a statistically significant difference with p=0.001. Conclusions: The reduction in the saliva levels of paraoxonase in periodontitis patients has led to a variation leading to increased ROS and therefore periodontal breakdown. The variation in salivary paraoxonase levels before and after treatment can be taken as an indicator of success of periodontal therapy.