Saliva Secretion Research Articles

Inclusion of Red Macroalgae (Asparagopsis taxiformis) in Dairy Cow Diets Modulates Feed Intake, Chewing Activity and Estimated Saliva Secretion.

The current study assessed the effects of red macroalgae Asparagopsis taxiformis (AT)-included as an enteric methane inhibitor-in dairy cow diets on feed intake and eating-rumination behaviour. Fifteen early lactating Norwegian Red dairy cows were offered ad libitum access to drinking water and a total mixed ration (TMR) composed of 35% concentrate feed and 65% grass silage on a dry matter (DM) basis. The experiment lasted for 74 days with the first 22 days on a common diet used as the covariate period. At the end of the covariate period, the cows were randomly allocated into one of three dietary treatments: namely, 0% AT (control), 0.125% AT and 0.25% AT in the TMR. The TMR was offered in individual feed troughs with AT blended in a 400 g (w/w) water-molasses mixture. Eating-rumination behaviour was recorded for 11 days using RumiWatchSystem after feeding the experimental diets for 30 days. The 0.25% AT inclusion significantly reduced the DM intake (DMI). Time (min/d) spent on eating and eating in a head-down position increased with the increasing AT level in the diet, whereas rumination time was not affected. The greater time spent on eating head-down with the 0.25% AT group resulted in a significantly higher chewing index (min/kg DMI). Estimated saliva production per unit DMI (L/kg DMI, SE) increased from 10.9 (0.4) in the control to 11.3 (0.3) and 13.0 (0.3) in the 0.125% and 0.25% AT groups, respectively. This aligned with the measured ruminal fluid pH (6.09, 6.14, and 6.37 in the control, 0.125% AT and 0.25% AT groups, respectively). In conclusion, either the level of the water-molasses mixture used was not sufficient to mask the taste of AT, or the cows used it as a cue to sort out the AT. Studies with relatively larger numbers of animals and longer adaptation periods than what we used here, with varied modes of delivery of the seaweed may provide novel strategies for administering the additive in ruminant diets.

The Physiology of Salivary Secretion and Its Role in Tooth Decay

The salivary fluid is produced by the major salivary glands like parotid, submandibular and sublingual glands along with minor salivary glands present in the oral cavity. Almost 0.5 to 1.5 liters of salivary secretion occurs in 24 hours. The salivary gland is under autonomic control but various other factors influence the rate and quality of the secretions. The mechanism of secretion of saliva involves a number of pumps and carrier proteins present in the acinar and ductal region of the gland that forms primary secretions which undergoes changes as it moves through the ducts and converts into secondary secretions. These salivary secretions have an important digestive, protective, anti-microbial, buffering function that protects teeth from dental caries.

Evaluation of Aedes aegypti Penetration, Probing, and Feeding Times on Mice.

In mosquitoes, the intradermal search for vertebrate blood (probing time) corresponds to the time taken from initial insertion of the mouthparts in the skin until visualization of the initial engorgement of blood in the midgut. Probing time evaluation provides useful information on the ability of a mosquito to initiate successful blood feeding. In this protocol, we describe how to determine feeding parameters in Aedes aegypti, a widely distributed mosquito that transmits several deadly pathogens, including yellow fever, dengue, Zika, and Chikungunya viruses. We focus on the different steps of a blood feeding event, including penetration, probing, interprobing, and feeding time. Penetration time corresponds to the insertion of the stylets into the host skin and usually lasts <10 sec. Probing time or intradermal search for blood involves saliva secretion into the skin. Some researchers group penetration and probing time as the exploratory phase for blood. Feeding time is an active phase of blood ingestion and engorgement. Feeding parameters depend on mosquito behaviors and these measurements are visually taken by the investigator. We include a video that provides a close look at a mosquito feeding event in which penetration, probing, and feeding times can be observed. To record these experimental times, one must closely watch the mosquito feeding behavior including stylet penetration in the host skin, visualization of the first traces of blood in the midgut, engorgement of the midgut, and removal of stylets from the skin.

The Parasympathetic Root of the Submandibular Ganglion: A Review.

The submandibular ganglion is a small fusiform-shaped cluster of cell bodies of the parasympathetic nervous system. Parasympathetic innervation of the submandibular gland is not only responsible for the secretion of saliva, but it also plays a main role in the development and regeneration of the gland. The parasympathetic root of the submandibular ganglion or the posterior branch of the lingual nerve to the submandibular ganglion is one of three roots of the submandibular ganglion. Using standard search engines (PubMed, Google), papers in English discussing the anatomy, embryology, variations, and clinical significance of the parasympathetic root of the submandibular ganglion were reviewed.

What is the food like that people choke on? A study on food bolus physical properties under different in vitro oral capacities

People with oral impairments, such as poor denture status, poor muscle strength, and poor salivary secretion, have more difficulties performing oral processes, which results in the risk of choking. In this study, we aimed to understand, in vitro, how different oral impairments can affect the oral processing of food reported as a choking hazard. Six foods that frequently cause choking were selected and studied, varying three in vitro factors at two levels—saliva incorporation amount, cutting activity, and compression action. The median particle size (a50) and the particle size heterogeneity (a75/25) of the food fragmentation, the hardness, and adhesiveness of the bolus formation, and the final cohesiveness of the bolus were studied. The results showed that all the parameters studied varied depending on the food product. High compression reduced a50 (except in mochi that increased) and a75/25 (except in eggs and fish) but increased bolus adhesion and particle aggregation (except for mochi). Regarding cutting activity, when performing a greater number of strokes, the particle size for sausage and egg, and the hardness of the bolus for mochi and sausage were lower. In contrast, for some food products, the bolus adhesiveness (bread) and particle aggregation (pineapple) were higher at a high number of strokes. The amount of saliva also played an important role in the creation of the bolus. When high amounts of saliva were added, the a50 values (mochi) and hardness (mochi, egg, and fish) decreased; and increased the adhesiveness (mochi) and particle aggregation (bread, pineapple, and sausage). When all oral factors are compromised (lack of muscle strength, denture status, and saliva secretion), some food products create a choking hazard as individuals cannot achieve the right particle size, bolus cohesiveness, and mechanical properties of the bolus to be safe to swallow, there is still a need to elaborate a guide, considering all the safety parameters.

Aquaporin 5 in Alzheimer's disease: a link between oral and brain pathology?

Aquaporin 5 in Alzheimer's disease: a link between oral and brain pathology?

Effects of polyphenols in non-centrifugal cane sugar on saliva secretion: in vitro and in vivo experiments and a randomized controlled trial.

This study examined the bioactivities and mechanisms of the non-centrifugal cane sugar polyphenols saponarin, schaftoside, and isoschaftoside in the salivary gland and their effects on salivation. In acute isolated C57BL/6N mouse submandibular gland cells, these polyphenols led to a higher increase in intracellular calcium after stimulation with the muscarinic agonist carbachol. Stimulation of these cells with polyphenols enhanced ATP production, aquaporin-5 translocation to the plasma membrane and eliminated intracellular reactive oxygen species generated by H2O2. In addition, phosphorylation of endothelial nitric oxide synthase and increased nitric oxide production in vascular endothelial cells were observed. In vivo administration of these polyphenols to C57BL/6N male mice resulted in significantly increased blood flow (saponarin, p = 0.040; isoschaftoside, p = 0.010) and salivation (saponarin, p = 0.031). A randomized controlled trial showed that intake of non-centrifugal cane sugar significantly increased saliva secretion compared with placebo (p = 0.003). These data suggest that non-centrifugal cane sugar polyphenols affect several pathways that support salivation and increase saliva secretion by enhancing vasodilation. Hence, non-centrifugal cane sugar polyphenols can be expected to maintain saliva secretion and improve reduced saliva flow.

History of xerostomia

Xerostomia means a decrease in saliva secretion and dryness of the oral mucosa. If the quality or quantity of saliva deviates from normal, it may be due to local or systemic disturbance with local and or systemic effects. This is a part of the Encyclopedia of the History of Dentistry.

Oral Health and Wellbeing of Patients with Idiopathic Pulmonary Fibrosis

Abstract Background Idiopathic pulmonary fibrosis (IPF) is a chronic progressive lung disease that significantly impacts everyday life. To date, little is known about oral cavity health in patients with IPF. Aim The study aimed to evaluate the condition of the oral cavity in patients with IPF and to assess their quality of life concerning oral cavity health. Methods The study was a cross-sectional one, collecting clinical data of patients with IPF and control subjects without pulmonary disorders. Data collection included baseline characteristics, oral cavity inspection results, and 12 answers provided in the GOHAI questionnaire. Periodontal status was investigated, and PD, CAL, BoP, API, CPITN and OHI were analyzed. Saliva secretion, halitosis, and oral lesions were also assessed. Results A total of 85 subjects – 40 patients with IPF and 45 controls – were enrolled in the study. In patients with IPF, both lower PD [0.54 (0.36–0.96) vs. 1.1 (0.6–1.44); p=0.001] and CAL [0.54 (0.3–1.26) vs. 1.43 (1.04–1.91); p=0.006] were found, and those patients also had lower saliva secretion [1.08 (0.33) vs. 1.28 (0.39); p=0.01]. Patients with IPF received periodontal treatment less frequently [2 (2.24%) vs. 15 (17.6%); p=0.001], and dental instruction was shown to them less often compared to the controls [13 (32.5) vs. 26 (57.78); p=0.02]. Moreover, IPF patients had significantly higher GOHAI scores [50.68 (5.24) vs. 44.37 (5.94); p&lt;0.001] compared to the control subjects. Conclusions Our study findings prompt the need for regular dental consultations and careful observation of dental prophylaxis in patients with IPF. The differences noted in the quality of life assessed using a GOHAI questionnaire need a further validation in larger cohort studies.

Aquaporins in Digestive System.

In this chapter, we mainly discuss the expression and function of aquaporins (AQPs) expressed in digestive system. AQPs are highly conserved transmembrane protein responsible for water transport across cell membranes. AQPs in gastrointestinal tract include four members of aquaporin subfamily: AQP1, AQP4, AQP5, and AQP8, and three members of aquaglyceroporin subfamily: AQP3, AQP7, and AQP10. In the digestive glands, especially the liver, we discuss four members of aquaporin subfamily: AQP1, AQP4, AQP5, and AQP8, three members of aquaglyceroporin subfamily: AQP7, AQP9, and AQP12. In digestive system, the abnormal expression of AQPs is closely related to the occurrence and development of a variety of diseases. AQP1 is involved in saliva secretion and fat digestion and is closely related to gastric cancer and chronic liver disease; AQP3 is involved in the diarrhea and inflammatory bowel disease; AQP4 regulates gastric acid secretion and is associated with the development of gastric cancer; AQP5 is relevant to gastric carcinoma cell proliferation and migration; AQP7 is the major aquaglyceroporin in pancreatic β cells; AQP8 plays a role in pancreatic juice secretion and may be a potential target for the treatment of diarrhea; AQP9 plays considerable role in glycerol metabolism and hepatocellular carcinoma; Studies on the function of AQP10 and AQP12 are still limited. Further studies are necessary for specific locations and functions of AQPs in digestive system.

PS-BPR02-8: THE EFFECTS OF AN ORAL FUNCTION IMPROVEMENT PROGRAM INVOLVING CHEWY KELP TEXTURE FOR LOCAL ELDERLY JAPANESE PEOPLE: A PILOT STUDY

Objective: The purpose of this study is to verify the efficacy and sustainability of an oral function improvement program involving chewy kelp texture in nutrition designed specifically for an elderly Japanese population. Study Design: A small pilot study Method: We developed a chewy kelp nutritional formula through the collaboration of geriatric nursing specialists and the professional staff of a seaweed processing company. The program to improve oral function consisted of a) salivary gland massage and swallowing exercises three times daily before each meal, followed by b) training to chew and eat chewy kelp. The program was conducted for two weeks, and such vital signs as blood pressure (BP), pulse, saliva secretion (relative value of average oral water content), tongue pressure, and occlusal force (glucose elution amount) were measured before and after the intervention. Results: 10 participants (4 male / 6 female: mean age 74.7 ± 5.4 years) enrolled. The program implementation rate of the participants was 93.6%. Measurements before and after the intervention included mean systolic BP (before, after, p) (138.0 ± 20.0, 131.0 ± 16.4 mmHg, p = .177), mean diastolic BP (79.6 ± 12.6, 73.2 ± 6.6 mmHg, p = .075), mean pulse (75.7 ± 11.0, 74.1 ± 10.8 times / min., p = .577), average oral mucosal moisture relative value (28.2 ± 4.9, 25.9 ± 3.3, p = .160), mean tongue pressure (29.6 ± 6.7, 32.3 ± 7.1 kPa, p = .213), and occlusal force (199.7 ± 78.9, 183.2 ± 42.7 mg / dL, p = .254). Among the participants, in one case in which occlusal function had significantly reduced, both mean systolic and diastolic BP decreased, and both tongue pressure and occlusal force increased. Conclusion: The high implementation rate indicated the potential sustainability of this program. Nevertheless, this study had certain limitations. First, the number of participants in the pilot study was small, falling short of the customary statistical sample size. In addition, because the implementation period was limited, any long-term effects of the program were not yet possible to show. Among preliminary findings of the study, for participants with impaired occlusal function, oral function may be improved and BP stabilized. Since periodontal disease is highly associated with cardiovascular disease and such side effects of hypertension drugs as increased thirst, it can be expected that longer trials of this program may see increased saliva production and improved oral function in participants.

Effect of increased saliva secretion on chewing and swallowing behaviors

Effect of increased saliva secretion on chewing and swallowing behaviors

Effects of Heat-Not-Burn Cigarette Smoking on the Secretion of Saliva and Its Innate Immune System Components.

Saliva and salivary antimicrobial proteins play important roles in the innate immunity, which prevents infections of orally invading bacteria and viruses. In this study, we compared the secretion rates of salivary lactoferrin (Lac) and lysozyme (Lys) in heat-not-burn (HNB) cigarette smokers and non-smokers. The analysis population for this study included 212 members of the fire department, including 32 HNB cigarette smokers, 17 paper cigarette smokers, 14 combined HNB and paper cigarette smokers, and 149 non-smokers. Salivary Lac and Lys concentrations were assessed using enzyme immunoassay. Saliva secretion was significantly lower among HNB cigarette smokers (p < 0.01) than among non-smokers. Accompanying this result, salivary Lac and Lys secretion rates were significantly lower among smokers, particularly HNB cigarette smokers, than among non-smokers (all p < 0.01). Our findings suggest a possible adverse effect of HNB cigarette on the amount of Lac and Lys released into the oral cavity.

The Effect of Administering Vitamin C Lozenges Towards the Increasing of Saliva Secretion of Hemodialysis Patient

Hemodialysis is a dialysis method to secrete bodily fluid when the kidneys are progressively or acutely unable to do it. This research aims to know the effect of administering vitamin C lozenges on the increasing saliva secretion of hemodialysis patients. The research used an experimental design with one group pre-test and post-test design. The T-test result was mostly dependent (paired sample test). A purposive sampling technique was used to acquire 30 hemodialysis patients. The result shows that 26 patients (86.7%) have an increase in saliva secretion while four patients (13.3%) have not. The p-value was 0.000 (p&lt;0.05). It can be concluded that administering vitamin C lozenges to hemodialysis patients increases saliva secretion. This research suggests that Royal Prima Medan Public Hospital administer vitamin C lozenges as an alternative medicine to increase the saliva secretion of hemodialysis patients.

Proton Pump Inhibitor Treatment Has Little Effects on Secretion of Saliva in Patients with Proton Pump Inhibitor-Responsive Mild Reflux Esophagitis and Non-Erosive Reflux Disease

Introduction: The secretion of saliva, which is triggered by acid reflux into the esophagus via the esophagosalivary reflex, plays a crucial role in the defensive mechanisms of the esophagus. The volume of saliva secreted in patients with gastroesophageal reflux disease (GERD) is reduced. However, the effects of proton pump inhibitors (PPI) on the secretion of saliva have rarely been reported. Therefore, the present study investigated changes in the volume and pH of saliva after the cessation of PPI. Materials and Methods: We retrospectively reviewed the records of consecutive patients previously diagnosed with mild reflux esophagitis (RE) or non-erosive reflux disease (NERD) controlled with PPI (including vonoprazan) who performed the salivary secretion test before and after a 2-week cessation of PPI. The volume, pH, and pH after acid loading (buffering capacity) of saliva were compared before and after the cessation of PPI. Results: Thirty-two patients (25 NERD, 7 mild RE) were included. The second saliva test was performed a median interval of 14 months [12.0–15.3] after the first test. No significant differences were observed in the volume of saliva secreted before and after the cessation of PPI (before 4.0 mL [2.7–6.0] vs. after 4.0 mL [2.3–5.9], p = 0.894). No significant differences were noted in pH or changes in pH after acid loading before and after the cessation of PPI (pH: before 7.1 ± 0.24 vs. after 7.0 ± 0.24, p = 0.1. Delta pH after acid loading: before 1.0 [0.8–1.2] vs. after 1.0 [0.8–1.2], p = 0.844). Conclusion: The cessation of PPI did not appear to affect the volume, pH, or buffering capacity of saliva in patients with PPI-responsive mild RE and NERD.

THE INFLUENCE OF NASAL BREATHING DISORDERS ON THE ORAL FLUID PARAMETERS IN PRESCHOOL CHILDREN

Dental caries is the most common dental disease in preschool children. Negative changes in the quantitative and qualitative parameters of oral fluid create optimal conditions for the development of cariogenic situation. One of the most important factors of oral cavity homeostasis changes in children of 3-6 years old is a persistent disorder in nasal breathing.&#x0D; The aim of the research is to determine the rate of saliva secretion and mixed saliva viscosity for their possible implementation as the biomarkers of risk of tooth caries appearence and justification of therapeutic and preventive measures prescription for elimination of the cariogenic situation in preschool children with persistent nasal breathing disorders. The rate of salivation and the viscosity of oral fluid were determined according to the method of Redynova T. L., Pozdeev A. R. (1994).&#x0D; The analysis of the obtained data on the rate of salivation of the examined children of the main and control groups revealed that the average value is within the normal range or at the lower margin of the parameter. A 1.5-fold decrease in the rate of salivation was found in children with persistent nasal breathing disorders compared to practically healthy children (р≤0.05). A more pronounced parameter decrease is observed in case of deciduous teeth presense. The average value of the viscosity of oral fluid is 2 times higher in children with persistent nasal breathing disorders than in the control group (р≤0.05). Higher viscosity of mixed saliva was found in children with caries than in children without it, but in the main group the numerical values were significantly higher (р≤0.05).&#x0D; The obtained data confirms the possibility of implementation of the determination of saliva secretion and viscosity rate as biomarkers of the tooth caries development in preschool children and provide an opportunity to control the application of therapeutic and preventive measures, considering the presence of nasal breathing disorders.

Application of Duplex Droplet Digital PCR Detection of miR-888 and miR-891a in Semen Identification.

To establish a system for simultaneous detection of miR-888 and miR-891a by droplet digital PCR (ddPCR), and to evaluate its application value in semen identification. The hydrolysis probes with different fluorescence modified reporter groups were designed to realize the detection of miR-888 and miR-891a by duplex ddPCR. A total of 75 samples of 5 body fluids (including peripheral blood, menstrual blood, semen, saliva and vaginal secretion) were detected. The difference analysis was conducted by Mann-Whitney U test. The semen differentiation ability of miR-888 and miR-891a was evaluated by ROC curve analysis and the optimal cut-off value was obtained. There was no significant difference between the dual-plex assay and the single assay in this system. The detection sensitivity was up to 0.1 ng total RNA, and the intra- and inter-batch coefficients of variation were less than 15%. The expression levels of miR-888 and miR-891a detected by duplex ddPCR in semen were both higher than those in other body fluids. ROC curve analysis showed that the AUC of miR-888 was 0.976, the optimal cut-off value was 2.250 copies/μL, and the discrimination accuracy was 97.33%; the AUC of miR-891a was 1.000, the optimal cut-off value was 1.100 copies/μL, and the discrimination accuracy was 100%. In this study, a method for detection of miR-888 and miR-891a by duplex ddPCR was successfully established. The system has good stability and repeatability and can be used for semen identification. Both miR-888 and miR-891a have high ability to identify semen, and the discrimination accuracy of miR-891a is higher.

Pharmacokinetic Basis for Using Saliva Matrine Concentrations as a Clinical Compliance Monitoring in Antitumor B Chemoprevention Trials in Humans

This study reports the first clinical evidence of significantly high secretion of matrine in a multi-component botanical (Antitumor B, ATB) into human saliva from the systemic circulation. This is of high clinical significance as matrine can be used as a monitoring tool during longitudinal clinical studies to overcome the key limitation of poor patient compliance often reported in cancer chemoprevention trials. Both matrine and dictamine were detected in the saliva and plasma samples but only matrine was quantifiable after the oral administration of ATB tablets (2400 mg) in 8 healthy volunteers. A significantly high saliva/plasma ratios for Cmax (6.5 ± 2.0) and AUC0-24 (4.8 ± 2.0) of matrine suggested an active secretion in saliva probably due to entero-salivary recycling as evident from the long half-lives (t1/2 plasma = 10.0 ± 2.8 h, t1/2 saliva = 13.4 ± 6.9 h). The correlation between saliva and plasma levels of matrine was established using a population compartmental pharmacokinetic co-model. Moreover, a species-relevant PBPK model was developed to adequately describe the pharmacokinetic profiles of matrine in mouse, rat, and human. In conclusion, matrine saliva concentrations can be used as an excellent marker compound for mechanistic studies of active secretion of drugs from plasma to saliva as well as monitor the patient's compliance to the treatment regimen in upcoming clinical trials of ATB.

Long-term functional regeneration of radiation-damaged salivary glands through delivery of a neurogenic hydrogel.

Salivary gland acinar cells are severely depleted after radiotherapy for head and neck cancer, leading to loss of saliva and extensive oro-digestive complications. With no regenerative therapies available, organ dysfunction is irreversible. Here, using the adult murine system, we demonstrate that radiation-damaged salivary glands can be functionally regenerated via sustained delivery of the neurogenic muscarinic receptor agonist cevimeline. We show that endogenous gland repair coincides with increased nerve activity and acinar cell division that is limited to the first week after radiation, with extensive acinar cell degeneration, dysfunction, and cholinergic denervation occurring thereafter. However, we found that mimicking cholinergic muscarinic input via sustained local delivery of a cevimeline-alginate hydrogel was sufficient to regenerate innervated acini and retain physiological saliva secretion at nonirradiated levels over the long term (>3 months). Thus, we reveal a previously unknown regenerative approach for restoring epithelial organ structure and function that has extensive implications for human patients.

Gland-sparing neck dissection: oncological and functional outcomes in oral cancer patients

This study was performed to evaluate the subjective and objective functional outcomes of patients who had undergone submandibular gland-sparing neck dissection. All data were obtained from patients treated in a single hospital. Seventy-seven patients who had undergone complete submandibular gland sparing (CSGS) were included in the study. Cancer prognosis items were recorded. The subjective outcomes included patient self-evaluation of mouth dryness and the evaluation of the presence of saliva secretion following the application of digital pressure. Saliva scintigraphy served as the objective test. Self-reported xerostomia was compared between the CSGS patients and a control group of patients who had undergone unilateral submandibular gland removal (USGR; n = 74). In the CSGS group, local recurrence occurred in 3.8% of the 80 cancer sites, and neck recurrence occurred in 5.9% of neck dissection sites. Regarding the subjective measurements, 7.0% of the CSGS patients reported xerostomia and 91.9% demonstrated saliva secretion by digital pressure. Scintigraphy revealed actively secreting glands, with 42.9% of them showing normal gland function; none of the patients had severe xerostomia. The relative risk of dry mouth was significantly higher in the USGR patients than in the CSGS patients (P < 0.001). Submandibular gland sparing during neck dissection was found to result in satisfactory saliva secretion, with a relatively small risk of local or neck recurrence.