Sagittaria Trifolia Research Articles

Resistance profile study of SU‐resistant Sagittaria trifolia in a comparison of seedlings and tuber‐derived plants

Resistance profile study of SU‐resistant <i>Sagittaria trifolia</i> in a comparison of seedlings and tuber‐derived plants

Plants at population edges enjoy longer mating distances: Implications for conservation practice

Habitat fragmentation and population declines have led to increased population edges, exposing higher proportions of plants to ecotones. However, it remains a challenging question how edge plants perform compared with interior ones, as they usually experience dissimilar abiotic and biotic interactions. To address this question, we performed experiments on a monoecious herb (Sagittaria trifolia) focusing on the reproductive fitness and mating patterns of individuals at the edge of two common gardens established under varied light conditions (open vs. understory) with natural pollination. Plant female fitness was determined by seed production, whereas male fitness and mating patterns were resolved by paternity analysis employing eight microsatellite markers. Results showed that edge plants had lower pollinator visitation frequency than interior ones in both gardens. However, there was no significant reduction in female or male reproductive success of edge plants. Instead, edge plants enjoyed longer distances of effective pollen dispersal when reproduced as males, as well as longer distances of sires when reproduced as females. This pattern was consistent across habitats and years. Together, the results indicate that edge plants not only maintained a comparable level of reproduction as interior ones but also enjoyed greater mating distances, which would likely bring about higher genetic diversity in their offspring. This further implies that edge effects may play an important role in promoting gene exchange and encouraging population recovery.

The Roles of Hormone Signals Involved in Rhizosphere Pressure Response Induce Corm Expansion in Sagittaria trifolia.

Soil is the base for conventional plant growth. The rhizosphere pressure generated from soil compaction shows a dual effect on plant growth in agricultural production. Compacted soil leads to root growth stagnation and causes bending or thickening, thus affecting the growth of aboveground parts of plants. In arrowhead (Sagittaria trifolia L.), the corms derived from the expanded tips of underground stolons are its storage organ. We found that the formation of corms was significantly delayed under hydroponic conditions without rhizosphere pressure originating from soil/sand. In the initial stage of corm expansion, the anatomic structure of arrowhead corm-forming parts harvested from hydroponics and sand culture was observed, and we found that the corm expansion was derived from cell enlargement and starch accumulation. Comparative transcriptome analysis indicated that the corm expansion was closely related to the change in endogenous hormone levels. Endogenous abscisic acid and salicylic acid concentrations were significantly increased in sand-cultured corms. Higher ethylene and jasmonic acid contents were also detected in all arrowhead samples, demonstrating that these hormones may play potential roles in the rhizosphere pressure response and corm expansion. The expression of genes participating in hormone signaling could explain the rising accumulation of certain hormones. Our current results draw an extensive model to reveal the potential regulation mechanism of arrowhead corm expansion promoted by rhizosphere pressure, which will provide important references for further studying the molecular mechanism of rhizosphere pressure modulating the development of underground storage organs in other plants.

Physiological and Biochemical Responses of Sagittaria trifolia L. to Phytotoxic Ethyl Acetate Fungal Extract from Curvularia lunata Strain CLST-01.

Curvularia lunata (No. CLST-01), a fungal pathogen isolated from the threeleaf arrowhead (Sagittaria trifolia L.), has been proposed as a potential mycoherbicide for grass weeds. This paper investigated the physiological and biochemical effects of CLST-01 phytotoxic ethyl acetate fungi extract on the leaves of the threeleaf arrowhead. The results showed that the ethyl acetate fungi extract from CLST-01 can accelerate damage to the cell membrane, increase the production of malondialdehyde, and damage the cellular structure, which could decrease the number of chloroplasts after 96 h treatments. In addition, the content of chlorophyll was reduced by 49.5%, and the net photosynthetic rate, stomatal conductance, and transpiration rate were inhibited. The rates of inhibition were 90.13%, 83.74%, and 79.31%, respectively, and the intercellular CO2 concentration increased by 51.87% on Day 9 after treatment with a concentration of 200 μg/mL. In summary, the phytotoxic ethyl acetate fungal extract from C. lunata CLST-01 can inhibit the photosynthesis of the threeleaf arrowhead leaves, destroy the ultrastructure of leaves, and affect the growth of this invasive weed. Therefore, it has the potential to be developed into a mycoherbicide for weed control in crops as a natural photosynthetic inhibitor.

Mutation at the 197 site and P450-mediated metabolic resistance are involved in bensulfuron-methyl resistance in Sagittaria trifolia

Sagittaria trifolia control is threatened by the emergence of resistance to acetolactate synthase (ALS)-inhibiting herbicides. Hence, we systematically uncovered the molecular mechanism of resistance to the main herbicide (bensulfuron-methyl) in Liaoning Province from target-site and non-target-site resistance perspectives. The suspected resistant population (TR-1) exhibited high-level resistance. A new amino acid substitution (Pro-197-Ala) in resistant Sagittaria trifolia for ALS was detected, and the molecular docking results showed that the spatial structure of ALS changed significantly after the substitution, manifested by an increase in the number of contacted amino acid residues and the disappearance of hydrogen bonds. Dose-response test of transgenic Arabidopsis thaliana further demonstrated that the Pro-197-Ala substitution conferred bensulfuron-methyl resistance. The assays found that the sensitivity of the ALS enzyme in TR-1 to this herbicide was decreased in vitro; and this population had developed resistance to other types of ALS-inhibiting herbicides. Furthermore, the resistance of TR-1 to bensulfuron-methyl was significantly alleviated after co-treatment with a P450-inhibitor (malathion). TR-1 metabolized bensulfuron-methyl significantly faster than sensitive population (TS-1) did, but this gap was narrowed after malathion treatment. Overall, the resistance of Sagittaria trifolia to bensulfuron-methyl was derived from the mutation of the target-site gene and the enhancement of the P450s-mediated detoxification metabolism.

Microwave-Assisted Enzymatic Extraction, Partial Characterization, and Antioxidant Potential of Polysaccharides from Sagittaria trifolia Tuber.

Sagittaria trifolia tuber is an aquatic vegetable. In this work, microwave-assisted enzymatic extraction (MEE) was used to extract S. trifolia tuber polysaccharides (STTPs). Optimum conditions were complex enzyme of 2 %, liquid-to-solid ratio of 43 : 1 mL g-1 , microwave power of 506 W, and time of 8 min, under which STTPs yield was 36.22±0.69 %, higher than those of other methods. STTPs were sulfated polysaccharides with sulfur valence of S6+ . STTPs comprised mannose, glucose, galactose, and arabinose at a mole ratio of 3.69 : 19.33 : 6.21 : 1.00, molecular weights of 3606 kDa and 149.6 kDa, particle size of 220 nm, and zeta potential of -5.02 mV. The surface of STTPs was full of bumps and holes, and abundant in O1s and non-functionalized C1s. STTPs would scavenge reactive oxygen species with advantage. It would provide an efficient MEE method to obtain antioxidant STTPs, also a clue for extracting polysaccharides from starch-rich crops.

Mutation at the 197 Site and P450-Mediated Metabolic Resistance are Involved in Bensulfuron-Methyl Resistance in Sagittaria Trifolia

Mutation at the 197 Site and P450-Mediated Metabolic Resistance are Involved in Bensulfuron-Methyl Resistance in Sagittaria Trifolia

КОНСПЕКТ ВЫСШИХ ВОДНЫХ РАСТЕНИЙ ОМСКОЙ ОБЛАСТИ

The article presents the results of generalizing the factual material on the diversity of hydromacrophytes from the divisions Marchantiophyta, Bryophyta and Magnoliophyta in the territory of the Omsk region. For each species the geographical frequency of occurrence in natural zones, the group of halobility and trophicity are indicated. For the region 176 species of higher hydromacrophytes have been identified. The division Magnoliophyta is distinguished by the greatest species diversity, which includes 116 species (65.9% of all higher hydromacrophytes), the division Bryophyta accounts for 49 species (27.9%), Marchantiophyta – 7 (4.0%), the divisions Equisetophyta and Polypodiophyta include only 2 species each (1.1%). Currently in the region 24 species of higher hydromacrophytes are included in the number of protected species with an established category of protection or as species that need special attention to their state and natural environment. In total, the following moss species are known from several habitats: Riccia fluitans, R. frostii, R. rhenana, Ricciocarpos natans, Fissidens adianthoides, Paludella squarrosa and vascular plants: Ceratophyllum platyacanthum subsp. oryzetorum, Elatine hydropiper, Veronica beccabunga, Najas marina, Sagittaria trifolia, Potamogeton rutilus, including brackish Ruppia maritima, Althenia orientalis, Zannichellia palustris var. repens.

Uptake Kinetics of \({\text{NH}_{4}^{+} }\), \({\text{NO}_{3}^{-} }\) and \({\text{H}_{2}\text{PO}_{4}^{-} }\) by Typha orientalis, Acorus calamus L., Lythrum salicaria L., Sagittaria trifolia L. and Alisma plantago-aquatica Linn

The emergent plants may differ in their capacity to assimilate nutrients from eutrophic water bodies, so the utilization of suitable emergent plants is the key part for successful restoration of shallow eutrophic lakes and rivers. This research applied the depletion method to study the kinetics of uptake of nutrient (H2PO4−, NH4+, NO3−) in different nutrient stresses by the five emergent aquatic plants (Acorus calamus L., Typha orientalis, Lythrum salicaria L., Sagittaria trifolia L., Alisma plantago-aquatica Linn) in the riverine zones of Dashi River (39°30′–39°40′ N, 115°59′–116°5′ E), a shallow eutrophic river located in Fangshan District, Beijing. The results showed that at the three phosphorus levels, A. calamus and A. plantago-aquatica had the highest maximum uptake rate values for NH4+ under low to moderate phosphorus conditions, and high phosphorus, respectively. T. orientalis had the highest maximum uptake rate values for NO3− at all phosphorus concentrations, while the Michaelis-Menten constant values of L. salicaria and A. plantago-aquatica were smaller. At the three nitrogen levels, the maximum uptake rate values for H2PO4− were the greatest for A. plantago-aquatica at the low to moderate nitrogen levels and L. salicaria at high levels. Meanwhile, T. orientalis and L. salicaria had the smallest Michaelis-Menten constant values. In this study, nitrogen microbial transformations, such as nitrification, denitrification and their coupling were not measured and their role in measuring kinetics was not assessed. Thus, achieved results shall be considered as a synthesis of several processes mediated by plants, a theoretical guidance to the selection of plant species for phytoremediation of polluted water bodies with different nutrient stresses for quality improvement around the diverse rivers in Haihe River basin.

野慈姑(Sagittaria trifolia)人工授粉的有效性:授粉工具与花粉保存

人工授粉作为一项关键性技术在生态研究与农业生产领域广泛应用，但是授粉过程中的工具有效性以及花粉保存尚未受到研究者的关注。以植物野慈姑为试验材料，研究了人工授粉方式和花粉保存条件对繁殖的影响。两年的结果表明，相较于直接接触式授粉，借助毛刷工具授粉显著降低了野慈姑的座果概率和种子数。花粉经过半小时野外高温保存后，其授粉的座果概率显著高于对照组，但单果种子数和种子大小保持稳定。综上，在野慈姑的人工授粉中应避免使用毛刷等工具，离体花粉可置于野外环境适当保存。在相关应用中，研究人员应当警惕工具授粉造成的负面影响，注意比较工具与其他授粉方式的等效性，保证实验的科学性。;Artificial pollination is a key technology with a wide range of applications in the fields of ecological research and agricultural practice. However, the effectiveness of pollination tools and pollen preservation has not received much attention. In this study, we investigated the effects of different artificial pollination methods and pollen storage conditions on the reproductive performance of Sagittaria trifolia. Results of two years showed that compared with direct contact pollination, pollination with brush tools significantly reduced fruiting probability and seed number in S. trifolia. The conditions of pollen handling also had significant effect on fruiting probability, in which half-hour field temperature storage had an advantage over control, whereas seed number per fruit and seed size remained stable. In summary, to aid artificial pollination in S. trifolia, pollination tools such as brushes should be avoided due to their negative effects on seed reproduction, and pollen in vitro can be preserved under field condition for an appropriate time. In related applications, researchers should be cautious of pollination tools and pay attention to the equivalence of different pollination methods prior to comparative experiments, in order to ensure scientific validity.

Sagittaria trifolia tuber: bioconstituents, processing, products, and health benefits.

Sagittaria trifolia is an aquatic plant that is distributed worldwide. The edible tuber part of S. trifolia is a very common and popular vegetable in China. The aim of the present review is to discuss the discovery of nutraceuticals from S. trifolia tuber by reviewing its major constituents, food processing, food products, and health-promoting benefits. Sagittaria trifolia tuber comprises a series of nutritional and bioactive constituents, including dietary fibers, amino acids, minerals, starches, non-starch polysaccharides, diterpenoids, colchicine, phenols, and organic acids. Food processing affects its flavor, biocomponents, and bioactivity. Numerous S. trifolia tuber-based food products and nutraceuticals have been developed, but new categories of products and the anticipated functions still need to be explored. The non-starch polysaccharides could be the central ingredients that contribute to the plant's antioxidant, hepatoprotective, hypoglycemic, lipid-regulating, and immunostimulatory properties. Of these, antioxidant and hepatoprotective effects have been thoroughly investigated. Procedures for the extraction and purification of polysaccharides influence their health-promoting actions. Overall, S. trifolia tuber is an underutilized aquatic vegetable species that is an emerging subject for nutraceutical research. © 2020 Society of Chemical Industry.

Leaf Transcriptome Analysis and Development of EST-SSR Markers in Arrowhead (Sagittaria trifolia L. Var. Sinensis)

As a native in Southeast Asia, Sagittaria trifolia L. var. sinensis (arrowhead) is an aquatic perennial herb of Alismataceae family. For the aim of discovering genes and development molecular markers, high-throughput transcriptome sequencing is applied to generate enormous transcript sequences from arrowhead leaf generate. Through trimmed and assembled, 51, 836 contigs were obtained with mean length of 680 bp. Sequence similarity analyses against four public databases (NR, GO, KEGG, KOG) found 23, 595 contigs that could be annotated with gene descriptions, conserved protein domains, or gene ontology terms. In this study, 3, 861 simple sequence repeat (SSR) markers of arrowhead were identified in 3, 174 contigs of transcriptome data,and 2, 476 primer pairs were designed for marker development. Of these, 100 primers were randomly selected, synthesized and used for validation of the amplification and assessment of polymorphisms in 79 arrowhead accessions. 78 loci reliably amplified a clear single band of expexted size, and 68 loci were scoreable and polymorphic with 2 to 11 alleles per locus. The polymorphism information content valued ranged from 0.01 to 0.84. The SSR markers developed from arrowhead, could be successfully applied to Sagittaria pygmaea, with a transferability rate of 91.14% (72/79). The phylogenetic tree was also constructed to analyse the genetic diversity in arrowhead accessions. The large number of SSR markers developed in this study will be useful in the researches of population genetics, genetic diversity, and germplasm characterization in arrowhead.

Soil seed banks and restoration potential of tussock sedge meadows after farming in Changbai Mountain, China

Vast tracts of the wettest agricultural fields have been abandoned worldwide, hence knowledge of their restoration potential is becoming important. Soil seed banks can be important components of ecological restoration, particularly if the seeds of key structural dominants can survive periods of cultivation. In Changbai Mountain, China, we compared the seed banks and standing vegetation between natural sedge meadows and adjacent paddy fields under drained and flooded conditions. The tussock-forming sedge Carex schmidtii dominated plant communities in the natural sedge meadows. However, this key structural dominant species was not found in the paddy fields. Other important wetland species survived cultivation as seeds (e.g. Cyperus fuscus, Lythrum salicaria and Sagittaria trifolia). Species of various life history types require either drawdown (emergent; e.g. C. schmidtii) or flooding (e.g. Potamogeton pectinatus) for successful germination. This study indicates that the seed banks of farmed sedge meadows could contribute towards the establishment of new wetland vegetation assemblages. Active revegetation of sedge meadow, particularly the native tussock-forming Carex, and changes in environmental conditions are necessary to support the region’s biodiversity.

Anti-proliferative effects of diterpenoids from Sagittaria trifolia L. tubers on colon cancer cells by targeting the NF-κB pathway.

A new labdane-type diterpenoid, ent-19-ol-13-epi-manoyl oxide,19-undecane ester, together with ten known diterpenes, were isolated from the ethanolic crude extract of the fresh tubers of Sagittaria trifolia L. The chemical structures of these compounds were determined by extensive 2-D NMR experiments and by comparison with the data reported in the literature. These compounds showed different inhibitory effects on various human cancer cells. Among these, compound 11 exhibited potential inhibition effects against human colon cancer cells. Moreover, flow cytometry demonstrated that compound 11 arrested the cell cycle at the G1 phase and induced cellular apoptosis, accompanied by mitochondrial membrane potential reduction. Mechanistic studies revealed that treatment with compound 11 inhibited IKKα/β phosphorylation and IκBα phosphorylation, which subsequently caused the blockage of NF-κB p65 phosphorylation and nuclear translocation. Compound 11 also inhibited the expression of c-Myc, Cyclin D1, and Bcl-2, the downstream targets of NF-κB. Therefore, our findings provided insight into the anticancer components of Sagittaria trifolia L. tubers, which could facilitate their utilization as functional food ingredients.

Floral finds in the city of Abakan (Republic of Khakassia, Russian Federation)

Abakan is a city district, the capital of a constituent entity of the Russian Federation of the Republic of Khakassia, with a population of 171.2 thousand people. On botanical and geographical zoning of L M Cherepnin Abakan is located in the Priabakanskaya valley steppe (S1) in the central part of the Khakass-Minusinsk depression at a height of 250 m above the sea level at the mouth of the Abakan river. Despite the fact that the territory of the Republic of Khakassia is studied well from the botanical point of view, there is not enough information about the current state, structure and species composition of the flora of the city of Abakan, which constituted the basis for in-depth studies of its vegetation cover. The aim of the work was to identify new and rare plant species in the flora of the city of Abakan. Floristic collections in the city were carried out from 2016 to 2018 by the method of model section of the urbanized landscape. As a result of the expeditionary research of 11 model sections, new plant species for the Republic of Khakassia (Centaurea ruthenica, Galium coriaceum, Myosotis sparsiflora, Oxytropis candicans, Peucedanum morisonii, Sagittaria trifolia, Silene turgida) and the city of Abakan were found and identified for the first time in the administrative boundaries of Abakan (Aquilegia grandulosa, Batrachium peltatum, Potamogeton crispus, Scirpus radicans, Viola canina). Habitats and model sections with the coordinates of the collection are specified, the boundaries of the ranges of species in the regions are marked.

The first complete chloroplast genomes of two Alismataceae species, and the phylogenetic relationship under order Alismatales

Alismatales play an important role in the systematics of angiosperm, yet different phylogenetic topologies of Alismatales based on different species have been generated from several studies. Here we reported and characterized the first complete chloroplast (cp) genomes of two Alismataceae species, Alisma plantago-aquatica and Sagittaria trifolia, and analyzed the phylogenomic relationship of Alismatales based on the complete chloroplast sequences of 21 Alismatales species. The two chloroplast genomes have the typical quadripartite structure with 113 genes in total, including 79 protein-encoding genes, 30 transfer RNA genes and four ribosomal RNA genes. Both IR regeions of plastid genomes contain 8 protein-encoding genes, 7 tRNA genes and all the four rRNA genes. The genome of A. plantago-aquatica is 177,417 bp in length, with an overall G + C content of 35.7%, and the genome of Sagittaria trifolia is 167,642 bp in length, with an overall G + C content of 37.0%. Phylogenetic study showed that the three Alismataceae species comprise a monophyletic group with a high support rate, which was a sister to the Hydrocharitaceae clade. The core Alismatids, consisting of Potamogetonaceae, Zosteraceae, Hydrocharitaceae and Alismataceae, is sister to Tofieldiaceae. Alocasia macrorrhizos of Araceae is the basal taxon of Alismatales, and the other twelve Araceae species formed a sister group to the clade Tofieldaceae and core Alismatids.

Sequential decline in fruit resource allocation within inflorescences of Sagittaria trifolia: a test of non‐uniform pollination hypothesis

AbstractFlowering plants often exhibit declining resource investment to floral organs, fruits and seeds temporally or spatially in an inflorescence. To account for such variances, non‐uniform pollination hypothesis, which highlights various mating environments each flower experiences, provides adaptive significance for allocation patterns but with controversial supports. Sagittaria trifolia (Alismataceae) was used to examine differences in seed number, seed weight and germination rate among sequential fruits within inflorescences. Ovule number was also investigated to evaluate allocation patterns in the floral stage. To test the non‐uniform pollination hypothesis, we used three polymorphic microsatellite loci of S. trifolia to estimate the seed outcrossing rate in proximal and distal fruits. The results showed that the seed number, average seed weight and seed germination rate of S. trifolia gradually decreased from proximal to distal fruits within inflorescences. The percent of decrease in seed number between two contiguous fruits was 14.68 ± 3.22%, which was much stronger than the percent of decrease in ovule number at 6.95 ± 1.60%. Both proximal and distal fruits within inflorescences had high outcrossing rates (81.5 ± 5.0%, proximal; 82.3 ± 6.9%, distal) and they did not differ significantly. Overall, there was an acropetal decline of resource allocation to fruits within inflorescences of S. trifolia. Allocation pattern to ovules was not a limiting factor for seed production. The lack of difference in outcrossing rate between proximal and distal fruits indicated that the allocation strategy was probably not caused by non‐uniform pollination, but more likely position effects.

Resource reallocation patterns within Sagittaria trifolia inflorescences following differential pollination

Understanding resource allocation to reproduction, a key factor in life history tradeoffs, has long intrigued plant ecologists. Despite the recognized importance of understanding the movement of resources among flowers following variable pollination, the patterns of resource reallocation to plant reproductive organs have not been thoroughly addressed. In this study, we aimed to empirically explore how resources redistribute within inflorescences in response to differential pollination intensities. Using a common herb, Sagittaria trifolia, we conducted supplemental and controlled pollination for single, some, or all flowers in simple and complex inflorescences, and compared their resulting fruiting probabilities, seed production, and average seed masses. Pollen supplementation of a single flower significantly increased its fruiting probability; however, the same manipulation of an inflorescence did not increase its overall reproduction. Single pollen-supplemented flowers had a higher percentage fruit set than inflorescences receiving supplemental pollination. In complex inflorescences, supplemental pollination had no effect on the reproductive success of flowers on the lateral or main branches. We provided evidence of resource reallocation from controlled to pollen-supplemented flowers in simple inflorescences; however, resources were unlikely to be reallocated between the main and lateral branches in the complex inflorescences, suggesting that flowering branches represent integrated physiological units in S. trifolia. The results also demonstrated that single-flower supplemental pollination would exaggerate pollen limitation and lead to a biased understanding of a plant's reproductive status.

The physicochemical characterization, equilibrium, and kinetics of heavy metal ions adsorption from aqueous solution by arrowhead plant (Sagittaria trifoliaL.) stalk

The arrowhead plant stalk (APS) has been investigated as a novel biosorbent for removal of Cd(II), Pb(II), and Cr(III) ions from aqueous solution. The surface physicochemical properties favorable for metals adsorption were systematically characterized. The Langmuir isotherm fitted well with Cd(II) and Pb(II) adsorption process onto APS while Dubinin–Radushkevich model best described Cr(III) sorption. The maximal adsorption capacities of APS for Cd(II), Pb(II), and Cr(III) were up to 38.2, 97.1, and 23.5 mg·g−1, respectively. The adsorption kinetic data of individual metal fitted the pseudo-second order model best. The adsorption of Cd(II) was exothermic, whereas the Pb(II) and Cr(III) underwent endothermic reaction. Overall, this investigation indicated, for the first time, APS is a potentially efficient biosorbent applied in Cd(II), Pb(II), and Cr(III) adsorption. It is also helpful for further utilizing the abundant quantity of APS which were abandoned in dietary arrowhead processing. Practical applications The growing food industry around world generates large quantity of by-products. The high value-added utilization of food processing by-products is one of the most important area in food industry. An attempt was made in present study to use the food by-product, arrowhead plant stalk (APS), as a novel biosorbent for removing Cd(II), Pb(II), and Cr(III) ions. In present study, it was evidenced that the adsorption capacities of APS for Cd(II), Pb(II), and Cr(III) were obviously higher than those of most reported agricultural by-products. This finding is significant for sustainable utilization of food crop arrowhead plant and elimination of environmental issues arising from the abandoned arrowhead plant stalk.

山形県に発生した除草剤抵抗性オモダカの遺伝子変異と各種除草剤成分に対する反応

山形県に発生した除草剤抵抗性オモダカの遺伝子変異と各種除草剤成分に対する反応