Rubrivivax Gelatinosus Research Articles

Effects of Temperature on the Characteristics of Nitrogen Removal and Microbial Community in Post Solid-Phase Denitrification Biofilter Process.

In order to solve the problems of high energy consumption, complex process and low nitrogen removal efficiency in the currently available low carbon source wastewater treatment processes, a novel coagulation sedimentation/post-solid-phase denitrification biofilter process (CS-BAF-SPDB) was proposed. The effect of temperature on the nitrogen removal performance of BAF-SPDB was intensively studied, and the mechanism of the effect of temperature on nitrogen removal performance was analyzed from the perspective of microbial community structure by using the polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that, to realize favorable nitrifying and denitrifying performance simultaneously in the BAF-SPDB unit, the operation temperature should be set above 18 °C. In addition, the influence of the macro operation parameters on the performance of the BAF and SPDB has a direct relationship with the dynamic changes of the micro microbial community. The influence of temperature on nitrification performance in BAF was mainly embodied in the change of composition, amount and activity of ammonia oxidizing bacteria Candidatus Nitrospira defluvii and nitrite oxidizing bacteria Nitrosomonas sp. Nm47, while that on denitrification performance in SPDB is mainly embodied in the change of composition and amount of solid carbon substrate degrading denitrifying bacteria Pseudomonas sp., Myxobacterium AT3-03 and heterotrophic denitrifying bacteria Dechloromonas agitate, Thauera aminoaromatica, Comamonas granuli and Rubrivivax gelatinosus.

Estimation of Dominant Bacterial Species in a Bench-Scale Shipboard Sewage Treatment Plant

Recently, an innovative method for wastewater treatment and nutrient removal was developed by combining the sequence batch reactor and membrane bioreactor to overcome pollution caused by shipboard sewage. This system is a modified form of the activated sludge process and involves repeated cycles of mixing and aeration. In the present study, the bacterial diversity and dominant microbial community in this wastewater treatment system were studied using the MACROGEN next generation sequencing technique. A high diversity of bacteria was observed in anaerobic and aerobic bioreactors, with approximately 486 species. Microbial diversity and the presence of beneficial species are crucial for an effective biological shipboard wastewater treatment system. The Arcobacter genus was dominant in the anaerobic tank, which mainly contained Arcobacter lanthieri (8.24%), followed by Acinetobacter jahnsonii (5.81%). However, the dominant bacterial species in the aerobic bioreactor were Terrimonas lutea (7.24%) and Rubrivivax gelatinosus (4.95%).

RETRACTED: Practicality of effluent containing Rubrivivax gelatinosus culturing the crucian carp

Abstract Application of traditional bait in freshwater aquaculture caused environment pollution and disease frequent occurrence. Effluent after Rubrivivax gelatinosus ATCC 49846 (R. gelatinosus) wastewater treatment could be re-utilized to culture Crucian carp as microbial feeds and aquaculture water. Therefore, a novel integrated system of wastewater treatment, the improvement of yield, digestion system, intestinal microbiota and aquaculture water quality of Crucian carp by effluent containing R. gelatinosus was proposed and investigated. Crucian carp could grow well in the effluent containing immobilized cells of R. gelatinosus ATCC 49846 in sodium alginate (ER) group. Survival rate and yield under ER group were almost same with control group (CK). The whole fish body composition under ER group were increased compared with CK. Biochemicals and remainder substances in effluent and R. gelatinosus enhanced protease, amylase, lipase activities. Theoretical analysis showed biochemical substances regulated these enzyme activities as component, active center. Moreover, R. gelatinosus and biochemical substances improved intestinal microbiota, the growth of intestinal probiotics and ATP production. Meanwhile, R. gelatinosus in effluent also purified aquaculture water quality. This technology would saved the water of aquaculture, reduced the water pollution and wastewater discharge, and improved the output, digestion system and intestinal microbiota of Crucian carp simultaneously.

RETRACTED: Effluent containing Rubrivivax gelatinosus promoting the yield, digestion system, disease resistance, mTOR and NF-kB signaling pathway, intestinal microbiota and aquaculture water quality of crucian carp

This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). This article has been retracted at the request of Editors-in-Chief and first Author. The article duplicates significant parts of a paper that had already appeared in Fish & Shellfish Immunology, Volume 93 (2019) 726-731, https://doi.org/10.1016/j.fsi.2019.06.052. One of the conditions of submission of a paper for publication is that authors declare explicitly that the paper has not been previously published and is not under consideration for publication elsewhere. As such this article represents a misuse of the scientific publishing system. The scientific community takes a very strong view on this matter and apologies are offered to readers of the journal that this was not detected during the submission process. The first author informed the journal that the article was published without the knowledge of the co-authors.

Production and use of microbial biomass helping sustainability in tilapia production chain.

In this study, the cultivation of Rubrivivax gelatinosus in fish industry effluent was carried out to cause the depollution of the by-product and generate a protein-carotenoid-rich biomass suitable to be used in tilapia feed. The bacterium was grown in the effluent (inoculum 1% v/v, 30 ± 5°C, 2000 ± 500lx, 7days) and recovered as a biomass (microfiltration + centrifugation + lyophilization), resulting in a decrease of ca. 80% in the chemical oxygen demand of the industry by-product and so putting it within the required limits for wastewater discard in Brazil. The biomass was characterized as a nontoxic product (behavior and physiology data) provided of nutritional and technological properties due to its composition-46% protein, 17% lipid, 5% minerals and 0.3% red oxycarotenoids. When cultured tilapias were fed the biomass (0, 175, 350, 700 or 1400mg/kg, 80days), their fillets got redder and had increased protein and carotenoid contents. Feeding the tilapias with the biomass also delayed fillet rancidity up to 80days of storage under freezing. Therefore, the biotechnological application of R.gelatinosus was demonstrated, providing a useful product and a service for the environment.

Inactivation of the uptake hydrogenase in the purple non-sulfur photosynthetic bacterium Rubrivivax gelatinosus CBS enables a biological water-gas shift platform for H2 production.

Biological H2 production has potential to address energy security and environmental concerns if produced from renewable or waste sources. The purple non-sulfur photosynthetic bacterium Rubrivivax gelatinosus CBS produces H2 while oxidizing CO, a component of synthesis gas (Syngas). CO-linked H2 production is facilitated by an energy-converting hydrogenase (Ech), while a subsequent H2 oxidation reaction is catalyzed by a membrane-bound hydrogenase (MBH). Both hydrogenases contain [NiFe] active sites requiring 6 maturation factors (HypA-F) for assembly, but it is unclear which of the two annotated sets of hyp genes are required for each in R. gelatinosus CBS. Herein, we report correlated expression of hyp1 genes with Ech genes and hyp2 expression with MBH genes. Moreover, we find that while Ech H2 evolving activity is only delayed when hyp1 is deleted, hyp2 deletion completely disrupts MBH H2 uptake, providing a platform for a biologically driven water-gas shift reaction to produce H2 from CO.

Dietary intake of Rubrivivax gelatinosus biomass enhances phagocytic cells in tropical fish Piaractus mesopotamicus infected with Aeromonas hydrophila

This study investigated the effects of prolonged administration of immunostimulant biomass produced by the bacteria Rubrivivax gelatinosus on the innate immune system of pacu Piaractus mesopotamicus infected with Aeromonas hydrophila. Fish were supplemented for 2 months with diets containing 0 g kg−1 biomass (TR1), 0.5 g kg−1 biomass (TR2) and 1.5 g kg−1 biomass (TR3). Haematological, biochemical and immunological analyses were carried out before biomass supplementation and 24 h after the triggering of its immune system with sub-lethal doses of A. hydrophila. As planned, no mortality was recorded in any group. After the bacterial challenge, fish from all groups showed a decrease (p < 0.05) in thrombocytes and lymphocytes and increase (p < 0.05) in monocytes, neutrophils and positive granular leucocyte (PAS-GL). Also, infected fish showed a decrease (p < 0.05) in glucose, protein, albumin and globulin and an increase (p < 0.05) in ALT and AST levels. Burst was higher (p < 0.05) after infection. The most relevant results were the increase (p < 0.05) of about 100% in monocytes and 50% in neutrophils in the group with the highest biomass supplementation compared with the control. These results suggest that incorporation of R. gelatinosus biomass into the diet for 60 days before exposure to a stress factor increases important cells involved in defence in the innate immune system.

Elucidating biochemical transformations of Fe and S in an innovative Fe(II)-dosed anaerobic wastewater treatment process using spectroscopic and phylogenetic analyses

An innovative process consisting of Fe(II)-dosed anaerobic bioreactors and an oxidizing basin was used to continuously treat a synthetic wastewater (COD/sulfate mass ratio 2:1 and Fe/S molar ratio 1:1). Sludge recycling effects were evaluated on ten occasions, in which anaerobic sludge was collected, biochemically oxidized with mechanical aeration in the oxidizing basin before being mixed with the wastewater influent. The sludge recycling resulted in better effluent quality compared to the baseline operation without recycling. More Fe and S were retained as sludge in the bioreactors with sludge recycling (Fe 94%, S 91%) than those when the bioreactors were operated without sludge recycling (Fe 76%, S 86%). Scanning electron microscopic analysis showed that bacterial cells and iron sulfide intermixed in the anaerobic sludge and the presence of microbial exopolymeric substances. X-ray spectroscopic analyses showed amorphous FeS formed from the dosed ferrous iron and biogenic bisulfide, and suggested long-term conversion of the amorphous FeS to more stable crystalline FeS and FeS2 in the anaerobic bioreactors. In the oxidizing basin, oxidation of iron sulfides was of both chemical and biological nature, and their oxidized forms including amorphous FeO/Fe2O3 mixture and partially/fully oxidized sulfurs. Experimental results also indicated amorphous FeS was more readily oxidized than FeS2. Phylogenetic analysis revealed microorganisms related to Desulfomonile tiedjei (sulfur reducing) and Alkaliphilus metalliredigens (iron reducing) in the anaerobic bioreactors, and Thiobacter subterraneus (sulfur oxidizing) and Rubrivivax gelatinosus (iron oxidizing) related microorganisms in the oxidizing basin.

Effects of Gas/Water Ratio on the Characteristics of Nitrogen Removal and the Microbial Community in Post Solid-Phase Denitrification Biofilter Process

To solve the problems of high-energy consumption, complex processes, and low nitrogen removal efficiency in the currently available low carbon source wastewater treatment processes, a novel coagulation sedimentation/post solid-phase denitrification biofilter process (CS-BAF-SPDB) was proposed. The effect of gas/water ratio on nitrogen removal efficiency of the CS-BAF-SPDB was studied. The changes in the microbial community structure along the varying gas/water ratio were investigated, and the functional bacteria were identified using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that, to realize favorable nitrifying and denitrifying performance simultaneously in the BAF-SPDB unit, the gas/water ratio of the BAF should be set at 4∶1. In addition, the influence of the macro operational parameters on the performance of the BAF and SPDB had a direct relationship with the dynamic changes in the micro microbial community. The influence of gas/water ratio on nitrification performance in the BAF was mainly embodied in the changes of composition, amount, and activity of ammonia-oxidizing bacteria Candidatus and Nitrospira defluvii and nitrite-oxidizing bacteria Nitrosomonas sp. Nm47, whereas that on denitrification performance in the SPDB was mainly embodied in the changes of composition and amount of solid carbon substrate degrading denitrifying bacteria Pseudomonas sp. and Myxobacterium AT3-03 and heterotrophic denitrifying bacteria Dechloromonas agitate, Comamonas granuli, and Rubrivivax gelatinosus.

Microbial biomass as an antioxidant for tilapia feed

Microbial biomass (MB) produced by different industries is thought to be a beneficial supplement in fish feed due to high contents of antioxidants and pigments. However, little is known about their impact on fish health. In this experiment, 960 tilapia (26.84 1.03 g) were fed one of eight experimental diets—a control diet with no MB (C), a control diet with vitamin E (VE) and six diets with three types of MB at two concentrations (0.25% and 0.5%): Rubrivivax gelatinosus (RG25 and RG50), Spirulina platensis (SP25 and SP50) and Saccharomyces cerevisiae (SC25 and SC50). Adding MB to diets decreased plasma total oxidant status, malonaldehyde and leucocyte respiratory burst; increased the total antioxidant status; and did not affect the blood biochemical parameters. In flesh, the use of the MB lowered the thiobarbituric acid-reactive substances and increased redness (except for SC) and carotenoid deposition (except SC25). So, it was concluded that the use of the MB provided an antioxidant effect in tilapia blood plasma, decreased lipid oxidation and increased pigmentation and carotenoid deposition in the fish flesh, without imparting a negative impact on the animals’ health.

Biogenesis of the bacterial cbb3 cytochrome c oxidase: Active subcomplexes support a sequential assembly model

The cbb3 oxidase has a high affinity for oxygen and is required for growth of bacteria, including pathogens, in oxygen-limited environments. However, the assembly of this oxidase is poorly understood. Most cbb3 are composed of four subunits: the catalytic CcoN subunit, the two cytochrome c subunits (CcoO and CcoP) involved in electron transfer, and the small CcoQ subunit with an unclear function. Here, we address the role of these four subunits in cbb3 biogenesis in the purple bacterium Rubrivivax gelatinosus Analyses of membrane proteins from different mutants revealed the presence of active CcoNQO and CcoNO subcomplexes and also showed that the CcoP subunit is not essential for their assembly. However, CcoP was required for the oxygen reduction activity in the absence of CcoQ. We also found that CcoQ is dispensable for forming an active CcoNOP subcomplex in membranes. CcoNOP exhibited oxygen reductase activity, indicating that the cofactors (hemes b and copper for CcoN and cytochromes c for CcoO and CcoP) were present within the subunits. Finally, we discovered the presence of a CcoNQ subcomplex and showed that CcoN is the required anchor for the assembly of the full CcoNQOP complex. On the basis of these findings, we propose a sequential assembly model in which the CcoQ subunit is required for the early maturation step: CcoQ first associates with CcoN before the CcoNQ-CcoO interaction. CcoP associates to CcoNQO subcomplex in the late maturation step, and once the CcoNQOP complex is fully formed, CcoQ is released for degradation by the FtsH protease. This model could be conserved in other bacteria, including the pathogenic bacteria lacking the assembly factor CcoH as in R. gelatinosus.

The biophysics of a critical phenomenon: colonization and sedimentation of the photosynthetic bacteria Rubrivivax gelatinosus.

In response to environmental changes, the photosynthetic bacterium Rubrivivax gelatinosus (Rvx.) can switch from a planktonic lifestyle to a phototrophic biofilm. Like in critical phenomena, the colonization and sedimentation of the cells is abrupt and hard to predict causally, and the underlying biophysics of the mechanisms involved is not known. Herein, we report basic experimental observations and quantitative explanations as keys to understanding microbial turnover of aggregates. (1) The moment of sedimentation can be controlled by the height of the tube of cultivation, by the concentrations of externally added Ficoll (a highly branched polymer) and/or of internally produced polysaccharides (constituents of the biofilm). (2) The observed translational diffusion coefficient of the planktonic bacteria is the sum of diffusion coefficients coming from random Brownian and twitching movements of the bacteria and amounts to 14 (μm)2/s. (3) This value drops hyperbolically with the association number of the cell aggregates and with the concentration of the exopolysaccharides in the biofilm. In the experiments described herein, their effects could be separated. (4) The critical conditions of colonization and sinking of the cells will be achieved if the height of the tube meets the scale height that is proportional to the ratio of the diffusion coefficient and the net mass of the bacterium. The decisive role of the web-like structure of a biofilm, the organization of bacteria from loose cooperativity to solid aggregation, and the possible importance of similar controls in other phototrophic microorganisms are discussed.

Rubrivivax gelatinosusbiomass as an immunostimulant for pacuPiaractus mesopotamicus

Rubrivivax gelatinosus is a bacterium present in the environment successfully used for treatment of fish industry effluent. Besides cleaning the effluent, this bacterium provides a biomass rich in proteins and carotenoid as a metabolic product. This study describes for the first time the use of this biomass as an immunostimulant feed. Haematological, immunological, biochemical and growth parameters in pacu Piaractus mesopotamicus fed control diet or diets supplemented with 0.5 or 1.5 g kg−1 of the R. gelatinosus biomass during 60 days were assessed. Fish were sampled at 0, 30 and 60 days. The inclusion of increasing levels of biomass in feed increased thrombocytes and neutrophils (P < 0.05) and decreased lymphocytes (P < 0.05) as well as activated the complement system (P < 0.05). Glucose levels, hepatosomatic index and weight were positively affected by the inclusion of R. gelatinosus biomass (P < 0.05). The use of R. gelatinosus biomass in feed improved the immune response of fish through the enhancement of some phagocytic cells, reduced time to activation of the complement system and increased growth parameters. This study reveals a promising use for a by-product resulted in the wastewater treating in aquaculture.

Effect of growth conditions on advantages of hup− strain for H2 photoproduction by Rubrivivax gelatinosus

H2 photoproduction by growing cultures of hup− mutant and parental strain RL2 of Rubrivivax gelatinosus was compared. We checked the influence of different substrates, presence of air and N2, culture shaking, inoculum concentrations. At low inoculum concentration, hup− strain demonstrated significant advantage over the parental strain in microaerobic conditions, while under N2–Ar atmosphere it was lower and vanished in anaerobic conditions (Ar only). This advantage was evident when using substrates with low degree of reduction (malate and succinate). Culture shaking under microaerobic conditions and in presence of N2 completely prevented H2 production by both strains. The high inoculum concentration inhibited H2 production under microaerobic conditions and in presence of N2, unlike to anaerobic conditions. With inoculum concentration increase, H2 production decreased not gradually but stepwise which means some metabolic shift. H2 production by hup− strain seems to be more tolerant to air traces than by parental strain.

Effects of the deletion of hup genes encoding the uptake hydrogenase on the activity of hydrogen production in the purple photosynthetic bacterium Rubrivivax gelatinosus IL144.

The efficiency of hydrogen gas production by nitrogenase in bacteria has been improved by the inhibition of antagonistic activity by the uptake hydrogenase. In this study, a mutant lacking the gene coding for the uptake hydrogenase was generated from the photosynthetic beta-proteobacterium Rubrivivax gelatinosus IL144 to explore new ways of hydrogen gas production driven by light energy. The mutant cells produced 25-30% higher amounts of molecular hydrogen than the wild-type cells under nitrogen-deficient conditions under light. Furthermore, by the addition of 5 mM glutamate, the photosynthetic growth rate was greatly enhanced, and the hydrogen gas production activity reached 41.1 (mmol/l) in the mutant.

Characterizations of purple non-sulfur bacteria isolated from paddy fields, and identification of strains with potential for plant growth-promotion, greenhouse gas mitigation and heavy metal bioremediation

This study was aimed at selecting purple non-sulfur bacteria (PNSB) isolated from various paddy fields, including Cd- and Zn-contaminated paddy fields, based on their biofertilizer properties. Among 235 PNSB isolates, strain TN110 was most effective in plant growth-promoting substance (PGPS) production, releasing 3.2 mg/L of NH4+, 4.11 mg/L of 5-aminolevulinic acid (ALA) and 3.62 mg/L of indole-3-acetic acid (IAA), and reducing methane emission up to 80%. This strain had nifH, vnfG and anfG, which are the Mo, V and Fe nitrogenase genes encoded for key enzymes in nitrogen fixation under different conditions. This strain provided 84% and 55% removal of Cd and Zn, respectively. Another isolate, TN414, not only produced PGPS (1.30 mg/L of NH4+, 0.94 mg/L of ALA and 0.65 mg/L of IAA), but was also efficient in removing both Cd and Zn at 72% and 74%, respectively. Based on 16S rDNA sequencing, strain TN110 was identified as Rhodopseudomonas palustris, while strain TN414 was Rubrivivax gelatinosus. A combination of TN110 and TN414 could potentially provide a biofertilizer, which is a greener alternative to commercial/chemical fertilizers and an agent for bioremediation of heavy metals and greenhouse gas mitigation in paddy fields.

Control of the lipid oxidation in Nile tilapia feed

ABSTRACT: The purpose of this research was to investigate the progress of the rancidity in Nile tilapia diets containing bacterial biomass of Rubrivivax gelatinosus . Six experimental treatments comprised basal diet (negative control), diet with asthaxanthin (positive control) and 4 diets with different concentrations of the bacterial biomass. The thiobarbituric acid assay for rancidity analyses were accomplished after 6 and 12 months of diets storage. It was concluded that Rubrivivax gelatinosus biomass minimized the racidity in Nile tilapia diets in 32.52 to 44.72% at 6 months and in 37.85 to 52.37% at 12 months of storage.

Composition of broilers meat

SUMMARY Brazil stands out in the ranking of broiler chicken production, holding the positions of the world's largest exporter and the third largest producer, scores that demand continuous attention to market requirements with regard to product quality, including the search for alternative sources of feed ingredients. This research's goal was to examine the effects of using Rubrivivax gelatinosus biomass in the feed of broilers on proximate composition, cholesterol content, and fatty acid profile of the meat cuts. Two hundred Cobb 500 male chicks were randomly assigned to one of the 4 dietary treatments containing zero, one, 2 or 3 g of dried R. gelatinosus biomass per kg of the finisher ration. At the end of the experiment, 20 birds from each treatment were slaughtered and their carcasses were cut into breast and thigh for the analyses of proximate composition, cholesterol, and fatty acids, all performed by standard methodologies. Data were analyzed by ANOVA and t test for the multiple comparisons of means using 5% of significance. Proximate composition and cholesterol concentration in both breast and thigh meats did not differ among treatments (P > 0.05). The biomass at 3 g/kg in the diet increased EPA in breast but decreased it in thigh. Nevertheless, no concentration of the biomass altered the ratio n-6/n-3 in either cut. Thus, the use of R. gelatinosus biomass at 3 g/kg in broilers’ rations was shown to be harmless or even beneficial to broiler meat composition.

Desempenho, sanidade animal e qualidade de filés de tilápias alimentadas com ração suplementada com biomassa bacteriana

A aquicultura moderna é um dos setores de produção de alimentos que mais cresce no mundo. A tilápia, além de possuir grandes vantagens produtivas, origina produtos com grande aceitação pelo mercado. Em sua nutrição, podem ser utilizados aditivos com finalidades zootécnicas, pigmentantes ou antioxidantes. Este estudo objetivou avaliar o efeito da suplementação da dieta de tilápias com biomassa de Rubrivivax gelatinosus sobre o desempenho zootécnico e a saúde dos animais (histologia e hematologia) e sobre as características de qualidade dos filés (pH, composição químico-bromatológica, cor e rancidez). O experimento contou com seis tratamentos, compostos de um grupo controle, sem aditivos, um grupo contendo pigmentante comercial e quatro grupos com a biomassa nas concentrações de 175, 350, 700 e 1400mg/kg. Peixes pesando 21,42±5,65g foram criados por 74 dias em sistema com recirculação de água e, posteriormente, foram abatidos para a realização das análises. Não foram encontradas diferenças para os resultados das análises de desempenho, histológicas e hematológicas. Os filés dos grupos alimentados com os aditivos apresentaram menor umidade que o grupo controle, e os filés dos grupos alimentados com biomassa apresentaram as maiores teores proteicos. Não houve diferenças entre os tratamentos para os valores de pH, lipídeos e cinzas. Quanto à cor dos filés, todos os tratamentos com aditivos aumentaram a intensidade de vermelho. Em todos os tratamentos, a rancidez dos filés foi crescente durante o armazenamento, embora em menores valores nos filés dos grupos tratados com as maiores concentrações de biomassa. A biomassa de R. gelatinosus não promoveu alterações no desempenho nem na saúde animal e mostrou-se capaz de melhorar os aspectos de qualidade e conservação dos filés.

Bacterial pigment for Nile tilapia feeding

The aim of this study was to evaluate the effects of different carotenoids sources in tilapia fish diets on the animals’ performance and fillets characteristics. Nine hundred and sixty tilapias, Oreochromis niloticus, averaging 15 g were distributed into 24 tanks to receive one of the six treatments (four repetitions) for 80 days: basal diet with no pigment (control group), basal diet with 350 mg/kg astaxanthin 10 % and basal diets added of four different concentrations of Rubrivivax gelatinosus biomass (175, 350, 700 and 1400 mg/kg). Variables analyzed included feed consumption, weight gain, feed conversion and specific growth rate for the animals and pH, proximate composition, carotenoids, polyunsaturated fatty acids and color for the fillets. Productive parameters did not differ statistically. Moisture content was lower on the fillets of treatments with pigments. The protein contents on the fillets of diets supplemented with the bacterial biomass were higher than in control group, while pH, minerals and lipids did not vary among treatments. Lightness and yellowness did not differ among the treatments, but redness and carotenoids contents were higher for all the groups that received the pigments than for the control group. The ratio of polyunsaturated fatty acids n-6/n-3 was improved with the dietary biomass. So, it was concluded that the use of the pigmenting ingredients did not alter productive parameters but increased redness and carotenoids contents in the fillets. Moreover, the use of R. gelatinosus biomass also increased the protein contents and improved the fatty acids profile in the fillets.