Abstract
In order to solve the problems of high energy consumption, complex process and low nitrogen removal efficiency in the currently available low carbon source wastewater treatment processes, a novel coagulation sedimentation/post-solid-phase denitrification biofilter process (CS-BAF-SPDB) was proposed. The effect of temperature on the nitrogen removal performance of BAF-SPDB was intensively studied, and the mechanism of the effect of temperature on nitrogen removal performance was analyzed from the perspective of microbial community structure by using the polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that, to realize favorable nitrifying and denitrifying performance simultaneously in the BAF-SPDB unit, the operation temperature should be set above 18 °C. In addition, the influence of the macro operation parameters on the performance of the BAF and SPDB has a direct relationship with the dynamic changes of the micro microbial community. The influence of temperature on nitrification performance in BAF was mainly embodied in the change of composition, amount and activity of ammonia oxidizing bacteria Candidatus Nitrospira defluvii and nitrite oxidizing bacteria Nitrosomonas sp. Nm47, while that on denitrification performance in SPDB is mainly embodied in the change of composition and amount of solid carbon substrate degrading denitrifying bacteria Pseudomonas sp., Myxobacterium AT3-03 and heterotrophic denitrifying bacteria Dechloromonas agitate, Thauera aminoaromatica, Comamonas granuli and Rubrivivax gelatinosus.
Highlights
In recent years, municipal domestic wastewater has become characterized by a low carbon to nitrogen (C/N) ratio [1]
This paper focuses on the influence of temperature on process’ nitrogen removal efficiency, and the changes of microbial community structure with temperature are studied by using a polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-Denaturing Gradient Gel Electrophoresis (DGGE)) technique to provide the microbiological basis for the research of process optimization and nitrogen removal mechanism
In order to study the influence of changing temperature on the nitrogen removal performance of coagulation sedimentation unit (CS)-biological aeration filter (BAF)-solid-phase denitrification biofilter (SPDB) process under low carbon conditions, influent ammonia concentration was kept at 30 mg/L, C/N ratio in the effluent of CS units was maintained at 3:1, gas/water ratio of BAF was set at 4:1, the hydraulic retention time (HRT) of BAF and SPDB
Summary
Municipal domestic wastewater has become characterized by a low carbon to nitrogen (C/N) ratio [1]. Temperature is one of the key factors affecting nitrogen removal efficiency of BAF-SPDB process [14,15] It affects the activity, specific growth rate and community structure of nitrifying and denitrifying microorganisms, and has a certain impact on the concentration of dissolved oxygen, and thereby has an impact on nitrification and denitrification. The start-up of this novel process and the effect of influent carbon/nitrogen ratio, gas/water ratio, hydraulic detention time and influent ammonia loading on the nitrogen removal efficiency of BAF-SPDB was investigated in the preliminary study in our laboratory [16]. This paper focuses on the influence of temperature on process’ nitrogen removal efficiency, and the changes of microbial community structure with temperature are studied by using a polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique to provide the microbiological basis for the research of process optimization and nitrogen removal mechanism
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