Routine Processing Research Articles

Development of a novel gene expression panel for the characterization of MSCs for increased biological safety.

Mesenchymal stromal cells (MSCs) have a wide range of therapeutic applications due to their multipotency, immunomodulatory, and anti-inflammatory properties. Their ability to migrate and recolonize damaged tissues is also remarkable. However, the controversial occurrence of spontaneous tumorigenesis or malignant transformation of MSCs raises concerns about proposed cell-based therapies for patients that researchers must address. There are several in vitro and in vivo strategies for MSC safety approval, but there is still no described coherent scheme that allows the assessment of MSC oncogenic potential in a simple, robust, and reproducible manner. Here, we have developed a diagnostic panel of molecular markers that allows for the accurate verification of the quality and safety of MSCs. Moreover, presented in this article diagnostic panel that can define the origin and tumorigenicity of MSCs can be easily introduced into the routine quality control processes of MSC-based product manufacturing which will improve further clinical applications of MSCs.

Effectiveness of a Novel Web-Based Intervention to Enhance Therapeutic Relationships and Treatment Outcomes in Adult Individual Psychotherapy: Randomized Controlled Trial and Analysis of Predictors of Dropouts.

Routine process and outcome monitoring interventions added to psychotherapy are known to improve treatment outcomes, although they vary in format and effectiveness. This study aimed to evaluate whether a therapist-independent, internet-based routine process monitoring and feedback system could significantly reduce psychological distress and enhance the quality of the therapeutic relationship compared with a treatment-as-usual control group among individuals already engaged in individual psychotherapy. We randomized 475 participants into either the intervention group, which received access to an internet-based routine process monitoring and feedback system in addition to psychotherapy, or the control group, which received only psychotherapy. The trial lasted for 10 weeks. Follow-up assessments at 5 weeks and 10 weeks used the Clinical Outcomes in Routine Evaluation-Outcome Measure as the primary outcome, with the Working Alliance Inventory-Short Revised and the Real Relationship Inventory-Client form as secondary outcomes. Per-protocol analyses (n=166) showed that psychological distress decreased in both groups, but there was no significant advantage for the intervention group. The intervention group experienced a decline in the genuineness dimension score of the real relationship, with an effect size of d=-0.27, compared with d=0.01 in the control group. In the intervention group (but not in the control group), dropouts showed significantly lower real relationship levels (P=.002), working alliance quality (P=.051), and emotional disclosure (P=.01) compared with those who completed the study. Additionally, logistic regression revealed distinct predictors of dropout within the control group and intervention group. The findings do not provide conclusive evidence for the efficacy of the new internet-based intervention in enhancing self-monitoring and prompting reflection on patients' emotional responses to their therapists. However, the intervention appears to influence patients' perceptions of the genuineness dimension in the therapeutic relationship, warranting further investigation. We hypothesize that this alteration in the genuineness dimension could be attributed to the intervention facilitating a more realistic and accurate perception of the therapeutic relationship among participants. ClinicalTrials.gov NCT06038747; https://clinicaltrials.gov/study/NCT06038747. RR2-10.2196/55369.

New Trends in Fashion Design: Digital Fashion Leads the Change

With the rapid evolution of information technology, the field of digital fashion has become a major driving force leading the innovation of clothing design. In doing so, this article will examine how digital technology can restructure the routine process of fabric design, enhance innovation effectiveness, deepen customer engagement, and promote sustainable development. First of all, this article traces the general process of classical tailoring, weighing the current status of digital technology in the field of fashion design, and the accompanying potential and dilemma. This article further elaborates on the unique use of cutting-edge technologies such as computerized design assistance systems (CAD), 3D printing, virtual reality (VR) and augmented reality (AR), and artificial intelligence (AI) in today's apparel innovation, showing how they are fundamentally disrupting the logical framework of design approaches, production models, and marketing. In addition, this study also focuses on the transformation of the consumer experience of digital fashion, including the online simulated fitting experience, personalized customized services, and the evolution of brand communication within the social media environment. On the topic of environmental protection and ethics, the paper explains how digital means can help to reduce the negative impact on the ecological environment, while also distinguishing the ethical boundary between virtual and physical dress, and the core value of information security and safeguarding the privacy of buyers. Finally, through the case study of the world's famous brands and new designers, the extensive role of digital fashion on the entire fashion design industry is summarized, and the prospect trend is prospected, providing practical guidance for experts and scholars in the industry. This research not only provides a theoretical basis for understanding the concept of clothing design in the digital era, but also contributes practical enlightenment to the practical level.

An Optimized Protocol for Multiple Immunohistochemical Staining of Fragile Tissue Samples.

Owing to the high occurrence of tissue detachment during the sample preparation process, the application of multiplex immunohistochemistry (mIHC) technology is limited in the field of fragile tissue samples, such as tendons, ligaments, and bones. To optimize a method for preparing sections for mIHC on fragile tissue samples, taking the human anterior cruciate ligament as an example, paraffin-embedded continuous sections with a thickness of 4 μm were divided into two groups: baking groups underwent routine section processing, and after being mounted on glass slides, they were baked at 65°C for 4 h, 8 h, or 24 h; ultraviolet (UV) photosensitive cross-linking groups used adhesive-coated slides for mounting and were directly subjected to UV light-induced cross-linking, with the cross-linking time set at 0 s, 20 s, 40 s, 1 min, 2 min, 3 min, 4 min, and 5 min, respectively. After deparaffinization and rehydration, we simulated the microwave step, which was most likely to cause tissue detachment during the mIHC experimental procedure, and then, the sections were stained with eosin. Finally, using the optimal cross-linking time selected from the UV cross-linking groups, mIHC staining of tendon and bone tissues was performed. After deparaffinization and rehydration, both groups were able to maintain the integrity of the tissue structure, except for the slides from the UV-sensitive cross-linking 0 s group, which showed complete tissue detachment. Following the seventh microwave treatment, the baking groups presented significant tissue detachment. The UV cross-linking groups were affected by the cross-linking time, and severe tissue detachment occurred with cross-linking times of 20 s, 40 s, and 5 min, whereas the tissues cross-linked for 1 min, 2 min, 3 min, and 4 min all maintained complete tissue morphology and structure. Finally, after 2 min of cross-linking, the results of spectral imaging revealed that the tissue morphology and structure were intact. During the process of mIHC staining, photocrosslinking with UV irradiation for 1-4 min effectively preserves the integrity of the tissue morphological structure.

SOCIO-CULTURAL DEVELOPMENT OF CHILDREN AS A BASIS FOR PREVENTING RISKS OF DIGITAL ADDICTION

The issue of digital addiction among children of different ages is becoming increasingly relevant. Digitalization is realized in the emergence of a new human habitat: virtual space, networked personality and psyche, internet thinking, a new type of communication landscape, etc. The assessment of the consequences of rapid immersion into this new reality for human culture is highly ambiguous. On the one hand, digitalization indeed signifi cantly streamlines and optimizes routine processes in various spheres of life, but on the other hand, digitalization rapidly alters the very way of life, subsequently affecting the psyche and personality of individuals (deontologization of space and changing the meanings of human life). Digitalization is not just a fundamentally new technology for organizing human life, but it entails such civilizational processes that can result in profound socio-cultural changes in the ontology of humankind. The aim of this review was to substantiate the role of socio-cultural development of children as the foundation for preventing the negative consequences of digitalization. Research on the attitudes towards digital devices among children of different ages and their parents indicates that children, teenagers, and young adults actively engaged with these devices exhibit personality traits that can be viewed as socio-cultural (intellectualization of the emotional sphere, specifi city of values and communications, more pronounced egocentricity, learned helplessness, etc.). Applied studies of attitude towards digital devices by children at different ages and their parents indicate that children, adolescents and young people involved in active interaction with digital devices are distinguished by personal characteristics that can be considered as socio-cultural (intellectualization of the emotional sphere, the specifi city of values and types of communication, more pronounced egocentricity, learned helplessness, etc.). At the same time, our research aimed at identifying the signifi cance of socio-cultural upbringing indicates the possibility of mitigating undesirable changes in the development of «digital» children. Socio-cultural education should be organized with a view to updating spiritual and moral meanings and values and taking into account the specifi cs of children’s subculture. The review considers variants of organization of socio-cultural development of children at different ages, as well as tools for the development of resistance to digital pressure.

Clinicians’ experiences of obtaining informed consent for research and treatment: a nested qualitative study from Pakistan

BackgroundInformed consent is considered to be the standard method for respecting the autonomy of individual participants in research and practices and is thought to be based on several conditions: (1) providing information on the purpose of the research or a specific treatment, what it will entail, (2) the participants being mentally competent to understand the information and weigh it in the balance, and (3) the participants to be free from coercion. While there are studies of informed consent in other countries, especially Low and Middle Income Countries (LMICs), this study explored the experiences of clinicians regarding the process of obtaining informed consent to participate in a Randomised Controlled Trial (RCT) in particular and treatment in general in healthcare settings, both general and mental health, specifically focusing on the tension between individualistic concept of autonomy and collectivist values in cultures such as Pakistan.MethodsQualitative interviews with 20 clinicians from healthcare settings in Pakistan who also served as recruiters in a suicide prevention RCT in Pakistan. The interviews were guided by semi-structured topic guide. All interviews were audio-recorded and transcribed verbatim.ResultsThe interviews revealed that shared decision making was more morally important than individual autonomy, the role of the family played a dominant part in the consent-taking procedure, the decision of the elder and/or family patriarch took prominence, and that clinician-researchers encountered significant challenges in consent process in Pakistan, while recruiting patients into the trial as well as during routine treatment processes in healthcare settings. Four distinct themes emerged which were (1) Family deciding for patients, (2) Benefits of involving family in consent process, (3) Gender disparity in consent process, (4) Challenges experienced by clinician-researchers during consent process in Pakistan.ConclusionsThe concept of consent is generally considered important in many cultures, however, there are two strands of understanding. There seems to be consensus that participant agreement is necessary to protect the participant but with regards to autonomy there are significant cultural differences whether it is the right for autonomy of the individual (individualistic concept) or family, community, or expert authority in other cultures. In Pakistan clinician-researchers sometimes preferred one approach and sometimes the other as they appreciated the interests of the patient to be.

Exploring legitimacy in a municipal budget decision in Switzerland: empirical insights into citizens' perceptions.

This study examines legitimacy in municipal budgeting decisions, focusing on input, throughput and output dimensions. Using data from four Swiss studies, we explore how citizens assess these dimensions across traditional and innovative decision-making processes and investigate the impact of different voting methods on legitimacy perceptions. Our findings reveal that in routine processes using traditional voting, legitimacy dimensions are considered collectively. Conversely, in innovative participatory budgeting, dimensions are judged separately, involving more active evaluation. Throughput legitimacy (perceived fairness) emerges as crucial in both contexts, while input and output legitimacy's importance varies by process type. The Method of Equal Shares voting system shifts focus towards procedural fairness, increases representation and is perceived as fairer than the traditional Greedy method. However, even fair processes cannot fully compensate for outcome dissatisfaction, highlighting the complex interplay of legitimacy dimensions. This research contributes to understanding legitimacy construction in municipal decision-making, offering insights into the relationship between voting methods and legitimacy perceptions. The findings have implications for policy-makers seeking to enhance the effectiveness and acceptance of budgeting processes. This article is part of the theme issue 'Co-creating the future: participatory cities and digital governance'.

DIGITAL TOOL FOR CALCULATING PROJECTED INITIAL RECOVERABLE OIL RESERVES AND OIL RECOVERY FACTOR

In today's realities, digitalization of production activities of companies and enterprises, including those involved in hydrocarbon production, is becoming increasingly important, allowing to create a single digital space for all employees, ensure transparency of the results obtained and provide prompt access to relevant information stored in a single database. The formation of a unified digital space implies the creation of an environment for employees, within which it is possible to use unified tools in solving production tasks. Data transparency eliminates the variability of results, demonstrating each step performed to achieve the result recorded in the database system. A unified database allows you to quickly familiarize yourself with all available relevant information, saving your employees time and effort. All these points are ultimately aimed at improving the efficiency of production processes, as well as optimizing the activities of employees working in different geographical locations, excluding from the work activities such routine processes as: manual recalculation of multiple indicators, search and review of information stored in different sources. Digitalization of production processes implies the use of digital technologies and tools formed on a certain technological basis. Based on the above, the authors have identified the criteria for the effectiveness of digital tools to perform production tasks: reducing the share of routine processes, providing digital transparency and storing results in a single database. The authors of the paper consider an example of digitalization of the oil recovery factor calculation process thanks to the implemented «Target Oil Recovery Factor» tool as part of the implementation of the RN-KIN Express corporate software.The purpose of the work under consideration is to confirm the performance of the implemented digital tool «Targeted EOR Express» (correct calculation of oil recovery factor and initial recoverable oil reserves) by means of retrospective analysis at fields in Western Siberia and Bashkortostan, and compliance with the stated criteria of an effective digital tool.Thanks to the retrospective analysis, the results confirming the accuracy of calculations of forecast values of residual recoverable oil reserves and oil recovery factor were obtained.Based on the results of the work, the implementation of «Targeted EORExpress» tool complies with the general trend of digitalization of production processes and includes all the criteria of an effective digital tool for production tasks.

A rapid response process for evaluating causes of extreme temperature events in the United States: The 2023 Texas/Louisiana heat wave as a prototype

Abstract As climate attribution studies have become more common, routine processes are now being established for attribution analysis following extreme events. This study describes the prototype process being developed through a collaboration across National Oceanic and Atmospheric Administration (NOAA), including monitoring tools as well as observational and model-based analysis of causal factors. The prolonged period of extreme heat in summer 2023 over Texas, Louisiana and adjacent areas provided a proving ground for this emerging capability. This event posed unique challenges to the initial process. The extreme heat lasted for most of the summer while most heat wave metrics have been designed for 3–7 d events. The eastern portion of the affected area also occurred within the so-called summer-time daytime warming hole where the warming trend in maximum temperatures has been mitigated wholly or in part by increased precipitation. The extreme temperature coincided with a strong—but not record—precipitation deficit over the region. Both observations and climate model simulations illustrate that the temperatures for a given precipitation deficit have warmed in recent decades. In other words, meteorological droughts today are hotter than their historical analogs providing a stronger attribution to anthropogenic forcing than for temperature alone. These findings were summarized in a prototype plain language report that was distributed to key stakeholders. Based on their feedback, the monitoring and assessment tools will continue to be refined, and the project is exploring other climate model large ensembles to increase the robustness of attribution for future events.

Role of Innate Lymphoid Cells on Laboratory Mice Routine Handling Process and their Effect on the Other Immunological Parameters

Introduction: Lab mice typically belong to the Mus musculus species. They are the prevailing choice for mammalian research and are utilized for studies in genetics, physiology, psychology, medicine, and various scientific fields. Aim of the study: The current research aimed to examine how frequently handling laboratory mice impacts their innate immune response through innate lymphoid cells, mucus secretion, as well as various physiological and biochemical blood parameters, and its potential implications on research outcomes. Study design: Sixty female mice were used in this study all animals were hosted under the same condition and treated with the same rotational animal handling process in different periods including animal control oral gavage and intraperitoneal, sub courteous, and intramuscular injection. Blood samples were gathered at four different time points to analyze various hematological and biochemical parameters, including (RBC, Hb, PCV, WBC, ALT, AST enzymes, and urea levels, etc.) while colon tissue were collected and the immunohistochemistry and PAS staining were preformed to evaluate the innate lymphoid cells and mucin secretion at three level through the experimental period. The results: Based on the findings, there were no notable variations in the levels of Hb and RBC. However, there was a noticeable increase in the WBC count for all animals after 4 minutes of handling, and the levels began to normalize after 10 and 30 minutes. As for the blood's biochemical parameters, including ALT, AST, creatinine, and urea, no details were provided. The results showed that there were no noteworthy alterations in these parameters throughout the entire experimental period. Conclusion: The handling of the animals is one of the most important factors that can influence the experimental results in lab mice. The WBCs and samples should be collected at least 30 minutes after the handling procedure.

Risk Assessment of Employing Digital Robots in Process Automation

Using digital technologies is essential to gain a competitive advantage in the global market by adapting to new business models. While digital technologies make business processes efficient, they enable companies to make faster and more accurate decisions by automating daily and routine process tasks. Robotic process automation (RPA) automates routine and repetitive business processes, allowing many jobs performed by humans to be performed faster. This way, advantages such as reduced error rates, reduced costs, increased production speed, and labor productivity are provided. For the successful implementation of RPA, potential risks need to be considered. In this study, failure mode and effect analysis (FMEA) based on decomposed fuzzy sets (DFSs), a new extension of intuitionistic fuzzy sets, has been used to evaluate subjectiveness in expert judgments. Differing from the other extensions of fuzzy set theory, the advantage of DFSs is to simultaneously consider decision-makers’ optimistic and pessimistic answers. Thus, the answer given by the decision-maker to the positive and negative questions on the same subject defines the indeterminacy of the decision-maker, and the method takes this indeterminacy into account in the evaluation. This study assesses and evaluates the potential risks of six digital robots in process automation. Thirteen risks were individually assessed for each automated process. This study found “Sustainability challenge” critical in three processes, “Absence of governance management” in two, and “Security“ in one. Variability in risk importance arose from process vulnerabilities.

Critical care resources in surge response: towards real-time situation awareness

ABSTRACT In large-scale emergencies and disasters, real-time situation awareness around health resources can be lifesaving. Understanding the situation at hand, the pressing needs and the actions needed often using information systems that require manual data entry. Relying on humans to enter data manually during surge response is both error-prone and an impediment to the critical requirement of real-time situation awareness. This paper explores the potential of Ultra-High Frequency Radio Frequency Identification (UHF RFID) technology to automate the process of manual data entry followed in providing situation awareness related to critical care resources during surge response. Using design science, an RFID-based prototype was developed to identify and track intensive care resources in real-time. The system was tested in a simulated hospital ward environment to understand deployment challenges and a dashboard was developed to reflect real-time data from multiple sources simultaneously. When properly deployed, UHF RFID can be a viable approach to automating critical care reporting during surge response. Automating routine processes can enhance data quality and minimise workload on health professionals leading to better patient care and evidence based decision-making.

“Too Much Trouble”: Transgender and Nonbinary People's Experiences of Stigmatization and Stigma Avoidance in the Workplace

Transgender and nonbinary (TNB) people in the U.S. navigate significant employment and economic inequities. Gaps in knowledge about their workplace experiences limit our broader understanding of how social inequality is interactionally constructed through employment contexts. We conducted and analyzed interviews with 26 TNB young adults. Routine hiring processes and structural constraints made participants vulnerable to interactional stigmatization and subsequent discrimination, with deleterious consequences for employment as well as mental health. Participants deployed a variety of strategies to avoid or resist anticipated stigma, including exiting the workforce or changing careers. One's ability to avoid stigmatization at work was partially shaped by structural and managerial support, organizational form, and one's gender and gender conformity. Beyond contributing to economic inequality by limiting job and career options, our findings suggest that these social processes comprise minority stressors that diminish the well-being of TNB participants and exacerbate their economic marginalization. In contributing empirical insight into the experiences of TNB people, we demonstrate the salience of stigmatization and stigma avoidance strategies for social closure within organizations and thereby advance sociological understanding of the relational generation of inequality at work.

Genome-Wide Profiling of the ACTIN Gene Family and Its Implications for Agronomic Traits in Brassica napus: A Bioinformatics Study

ACTINs are key structural proteins in plants, which form the actin cytoskeleton and are engaged in numerous routine cellular processes. Meanwhile, ACTIN, recognized as a housekeeping gene, has not yet been thoroughly investigated in Brassica napus. The current research has led to the detection of 69 actin genes in B. napus, which were organized into six distinct subfamilies on the basis of phylogenetic relationships. Functional enrichment analysis, along with the construction of protein interaction networks, suggested that BnACTINs play roles in Preserving cell morphology and facilitating cytoplasmic movement, plant development, and adaptive responses to environmental stress. Moreover, the BnACTIN genes presented a wide range of expression levels among different tissues, whereas the majority experienced a substantial increase in expression when subjected to various abiotic stresses, demonstrating a pronounced sensitivity to abiotic factors. Furthermore, association mapping analysis indicated that some BnACTINs potentially affected certain key agronomic traits. Overall, our research deepens the knowledge of BnACTIN genes, promotes the cultivation of improved B. napus strains, and lays the groundwork for subsequent functional research.

Standardized maps – an emerging approach to leverage quantitative information in knee imaging

ObjectiveProperty maps, which capture spatial variations across the entire joint, are emerging as a powerful means for extracting and analyzing quantitative information from knee 3D imaging datasets, particularly from CT and MRI data. This perspective paper aims to discuss the processing pipelines used so far, as well as the results they have enabled with respect to osteoarthritis. DesignThe key methodological steps for obtaining property maps, including segmentation, property calculation, and standardization are presented and analysis methods are discussed. Representative studies are also examined to illustrate the state-of-the-art in this field. ResultsThree main processing pipelines have been used, with the segmentation, property calculation, and standardization steps occurring in different orders. Many methods have been successfully considered for ordering these steps, without any looking generally preferable to the others. Thanks to recent advances in segmentation and standardization techniques, routine processing of property maps appears conceivable in the near future. Maps have been analyzed for multiple purposes, including group comparisons, pattern recognition, and cross-property modelling. Mostly maps of cartilage thickness and composition, as well as maps of bone shape and mineral density have been reported. They revealed distinct patterns associated with osteoarthritis severity, achieved high diagnostic accuracy, and identified relationships among tissue properties. ConclusionsProperty maps represent a promising approach for leveraging the extensive information in imaging data. They are particularly interesting for standardizing complex spatial variations in tissue properties, enabling global analysis and modelling. Once challenging to obtain and interpret, current mapping methods are being improved to the point that property maps may well be in routine use in the near future.

Implementation and improvement of a routine suicidal ideation screening process for an ambulatory, academic NCCN-designated center.

294 Background: From May – August 2021, outpatient suicidal ideation-related safety events nearly doubled from the three years prior at an academic NCCN center. This rise in events challenged staff and revealed improvement opportunities for suicide prevention, such as routine screening for suicidal ideation (SI) in the ambulatory setting. In addition, the COVID pandemic introduced an added layer of vulnerability to cancer patients, who are at an increased SI risk compared to the general population. Methods: From August 2021 - 2023, a multidisciplinary team developed and implemented a routine SI screening, intervention and escalation process that included a staff education program, a process-aligned EHR build, a metric dashboard, and a quality improvement-control plan. The vision was to identify all patients experiencing SI and support them with timely and compassionate care. A step-up metric target approach was used to set quarterly goals. The overarching goal was to screen at least 70% of eligible patients by the end of fiscal year 2024. The new process went live organization-wide on July 31, 2023. Following implementation, various improvement opportunities were identified with indicators including performance metrics, process audits, patient case reviews, and stakeholder feedback. Initial improvement efforts focused on clinics and sites that were screening < 25% between FY24 Q1 and Q2. Continued collaboration between Quality Improvement and clinic leaders revealed improvement opportunities in identifying eligible patients and staff screening practices. Impactful interventions addressed patient identification and staff coaching. Results: As a new process, the overall screening rate started at 0% and increased to 48% over FY24*. Conclusions: Overall, a new SI screening process was successfully implemented, and improved with diligent PDSA work, yet metric goals were not achieved. Many factors may have contributed to underperformance. Ongoing evaluation and collaboration with stakeholders are crucial for continued improvement. Beyond these efforts exists the social and cultural stigma of suicide, which may have limited the impact of improvement endeavors. Results may demonstrate further need for open and supportive discussion with staff, patients, and the larger healthcare community. Data time range: 7/31/23-5/21/24.* FY24 + Quarter Metric Goal Screening Rate Q1 40% 35.7% Q2 50% 34.5% Q3 60% 41.2% Q4 70% 47.8%

Use of Carrot Oil, Olive Oil, Pine Oil, Rose Oil in Comparison to Xylene as Clearing Agent During Tissue Processing: an in Vitro Study

ABSTRACT: Background: Clearing plays a vital role in histological section preparation. Xylene has long been valued for its ability to efficiently deparaffinize and clear tissue samples. However, its hazardous properties have raised health and environmental concerns, leading researchers to seek alternative solvents for tissue processing. This study utilized safer substitutes to address these concerns. Aim: To assess the clearing ability of four different oils i.e., Carrot oil, Olive oil, Pine oil, and Rose oil in comparison with that of xylene. Materials and Methods: Thirty different formalin-fixed tissue samples were cut into five parts and cleared using Carrot oil, Olive oil, Pine oil, Rose oil, and xylene followed by routine processing, sectioning, and staining steps. Presence or absence of shrinkage, ease of cutting, nucleus, cytoplasm details and staining quality were assesed and comparison was done between each of them cleared with the 4 different oils with that cleared using xylene. Results: The results showed that all four oils cleared tissues as effectively as xylene, with Pine oil superior in nucleus and cytoplasm details, as well as staining quality insignificant number of samples. Conclusion: These oils not only more affordable and eco-friendly than xylene but can also serve as effective clearing agents.

HIPI: Spatially resolved multiplexed protein expression inferred from H&E WSIs.

Solid tumors are characterized by complex interactions between the tumor, the immune system and the microenvironment. These interactions and intra-tumor variations have both diagnostic and prognostic significance and implications. However, quantifying the underlying processes in patient samples requires expensive and complicated molecular experiments. In contrast, H&E staining is typically performed as part of the routine standard process, and is very cheap. Here we present HIPI (H&E Image Interpretation and Protein Expression Inference) for predicting cell marker expression from tumor H&E images. We process paired H&E and CyCIF images taken from serial sections of colorectal cancers to train our model. We show that our model accurately predicts the spatial distribution of several important cell markers, on both held-out tumor regions as well as new tumor samples taken from different patients. Moreover, using only the tissue image morphology, HIPI is able to colocalize the interactions between different cell types, further demonstrating its potential clinical significance.

ЖАПОНИЯДАҒЫ САЛЫҚТЫҚ ӘКІМШІЛЕНДІРУДІ ЦИФРЛАНДЫРУ ТӘЖІРИБЕСІН ЗЕРТТЕУ

The tax policy of the state in conjunction with the methods of tax administration is a determining factor influencing the amount of tax revenues of the budget. Tax discipline, forms and close interaction between taxpayers and regulatory authorities also play a significant role. The development of information and communication technologies (ICT) in recent decades has provided the tax authorities with a wide range of opportunities to increase government revenue, improve the efficiency and quality of services provided to taxpayers, including electronic filing of returns and payment of taxes, while reducing the administrative burden on taxpayers and reducing public administration costs. The first phase of digitalization was the computerization of routine tax administration processes (such as taxpayer registration and income records), and in the second half of last decade and early this decade, many tax authorities adapted their websites to simplify operations by introducing online forms and calculators for taxpayers. In June 2017, Japan National Tax Agency published a strategic plan for the Japanese tax administration called "Looking to the Future." This includes the pursuit of sound taxation and good governance to ensure that taxes are paid based on the understanding and trust of taxpayers. The National Tax Agency of Japan is well aware of the importance of continually moving toward the future it seeks, emphasizing the goal of transparent tax administration. Keywords: tax and customs administration, OECD countries, tax and customs, administrative costs, Tax Code, tax authorities, taxpayers, tax relations, digitalization.

Diagnostic yield of exome sequencing-based copy number variation analysis in Mendelian disorders: a clinical application

Next-generation sequencing (NGS) coupled with bioinformatic tools has revolutionized the detection of copy number variations (CNVs), which are implicated in the emergence of Mendelian disorders. In this study, we evaluated the diagnostic yield of exome sequencing-based CNV analysis in 449 patients with suspected Mendelian disorders. We aimed to assess the diagnostic yield of this recently utilized method and expand the clinical spectrum of intragenic CNVs. The cohort underwent whole exome sequencing (WES) and clinical exome sequencing (CES). Using GATK-gCNV, we identified 12 pathogenic CNVs that correlated with their clinical findings and resulting in a diagnostic yield of 2.67%. Importantly, the study emphasizes the role of CNVs in the etiology of Mendelian disorders and highlights the value of exome sequencing-based CNV analysis in routine diagnostic processes.