Routine Processing Research Articles

Design and Development of Eco-Friendly Road Sweeper Machine

Abstract: This project is related to design and development of most effective machine that is manually operated mechanical pollution free road cleaner. The Road cleaner is used to keep our mother earth clean. So that we feel fresh while walking on streets. Generally, in era of modern technology, different devices such as electric motors, diesel engines and robots are being used to clean floor, road. These methods make much pollution, maintenance and very tough to carry out. The main objective of this paper is to spread this idea of our prototype road cleaner to each one which aims to. Hence, the present work is aimed to design and develop a manually operated road cleaning machine which is eco-friendly, cost effective, portable and less maintenance. Cleaning has become a basic need for all human beings and it is unavoidable in our daily routine process. The conventional floor/road cleaning machine is most widely used in railway stations, airports, hospitals, Bus stands, colleges etc. also this machine needs electrical energy for its operation. It is not user friendly as well as ecofriendly. In summer time there is power crisis and most of the floor/roads cleaning machines are not used effectively due to this problem particularly. In our project we are using easily available materials with low cost. It is the better alternative for conventional machine. Hence this project is very useful in our day to day life

Bodies in Motion: Self-care, Effort, and Discipline in an Ethnography with Weightlifting Practitioners

This article is part of a survey of young bodybuilders at a gym in Campo Grande, Mato Grosso do Sul, Brazil. It focuses on the discussion around exercises’ routine processes, motivations, perceptions, and representations of the corporeality of the practicing subjects, as revealed in observations and in-person conversations. Using ethnography as a method, the approach to the subjects took place through participant observation, in which the researchers took part in the daily routine of the gym. The results discussed here show that the gym is a space for social interaction, learning, and valuing discipline to build and maintain carved bodies.

Crack mitigation strategies for a high-strength Al alloy Al92Ti2Fe2Co2Ni2 fabricated by additive manufacturing

Laser powder bed fusion is capable of fabricating aluminum (Al) alloy parts with great geometrical flexibility and rapid prototyping for various industries. However, high strength Al alloys generally suffer from solidification cracks due to the steep thermal gradient associated with the laser fusion process. Here, we report several strategies to mitigate the hot crack susceptibility of a high strength Al92Ti2Fe2Co2Ni2 alloy. Routine processing parameter optimization based on varying laser power and scanning speed has to trade off porosity for producing crack-free parts, making it not suitable for load-bearing structural applications. Furthermore, secondary printing parameters, including laser strip length, laser defocus, scanning strategies, etc., improved printability but were insufficient to eliminate all the cracks. Crack morphology and residual stress measurements indicate that the cracks are generated in the solid state driven by large tensile residual stress, instead of solidification cracking or liquation cracking. Thus, an attempt was made to alleviate the residual stress in a controlled manner. By properly introducing a compliant, sacrificial, scaffold support structure to regulate crack propagation, near fully dense, crack-free parts could be successfully printed. The results were further verified by micro computed tomography, showing that cracking could be arrested in the support before propagating through the parts. This method can be readily applied to other alloy systems without modifying the chemistry.

Histopathological changes in the placentas of pregnant women with Gestational Diabetes Mellitus

Objectives: Placental damage might be the primary underlying cause of the high rates of fetal adverse outcomes in cases with gestational diabetes mellitus (GDM). Histopathological changes were considered to be associated with GDM in the literature. However, there are also studies showing no changes in the placenta in some gestational diabetic women. The pathophysiology of these placental alterations remains elusive. Therefore, this study aimed to examine the histopathological changes in the placentas of patients with GDM and compare them with the placentas of the healthy control group.
 Material and methods: This experimental non-randomized case-control study was conducted with 40 GDM patients and 40 healthy pregnant women at Dicle University Faculty of Medicine between March 2023 and December 2023. Placental tissues obtained following cesarean delivery were subjected to routine tissue processing. Placental samples were stained with hematoxylin-eosin and examined histopathologically using a light microscope.
 Results: When histopathological findings were compared between the GDM and control groups, villous degeneration, congestion in blood vessels, fibrinoid accumulation, inflammation and intervillous hemorrhage were higher in the GDM group than in the control group (p<0.001).
 Conclusion: The intricate relationship between GDM and placental pathology underscores the profound impact of metabolic changes on placental structure and function throughout pregnancy. Understanding these pathological changes is crucial for elucidating the progression of GDM and its implications for maternal and fetal health, highlighting the importance of comprehensive placental analysis in managing pregnancies complicated by GDM.
 Keywords: Gestational diabetes, hematoxylin-eosin staining, histopathological examination

High positive predictive value 22q11.2 microdeletion screening by prenatal cell-free DNA testing that incorporates fetal fraction amplification.

22q11.2 deletion syndrome (DS) is a serious condition with a range of features. The small microdeletion causing 22q11.2DS makes it technically challenging to detect using standard prenatal cfDNA screening. Here, we assess 22q11.2 microdeletion clinical performance by a prenatal cfDNA screen that incorporates fetal fraction (FF) amplification. The study cohort consisted of patients who received Prequel (Myriad Genetics, Inc.), a prenatal cfDNA screening that incorporates FF amplification, and met additional eligibility criteria. Pregnancy outcomes were obtained via a routine process for continuous quality improvement. Samples with diagnostic testing results were used to calculate positive predictive value (PPV). 379,428 patients met study eligibility criteria, 76 of whom were screen-positive for a de novo 22q11.2 microdeletion. 22 (29.7%) had diagnostic testing results available, and all 22 cases were confirmed as true positives, for a PPV of 100% (95% CI 84.6%-100%). This performance was based on cases that ranged broadly across FF (5.9%-41.1%, mean 23.0%), body mass index (22.3-44.8, mean 29.9), and gestational age at testing (10.0w-34.6w, median 12.7w). Ultrasound findings in screen-positive pregnancies were consistent with those known to be associated with 22q11.2DS. 22q11.2 microdeletion screening that incorporates FF amplification demonstrated high PPV across both general and high-risk population cohorts.

Digital marketing automation

Increased competition in an uncertain digital environment requires new approaches to improving marketing and business efficiency. Automation of business processes and marketing ensures management decisions aimed at improving customer experience by collecting, processing, and analyzing a huge amount of objective customer data from various sources and software services. For the company, it saves time and resources by automating routine processes, and, given the improved customer experience and corresponding loyalty of the target audience, it helps to increase the efficiency of marketing investments and improve the effectiveness of digital marketing (DM) and business. The article is aimed at substantiating the structure of a single user profile based on the integration of ESP and other DM software services with a CRM system. The hypothesis is formulated: creating a single user profile based on the integration of ESP and other DM software services with a CRM system by speeding up communication and improving customer experi­ence will increase the efficiency of DM and business in general. The research was conducted using general scientific and special methods: analysis and synthesis, system-structural analy­sis, comparison, systematic, logical generali­zation of results. Considerable attention is paid to email marketing, which remains one of the most popular DM automation tools, as it allows you to smoothly guide the consumer through the sales funnel with a high return on marketing investment. The effectiveness of integrating a CRM system and an ESP is proved, due to the specialization and functional features of the latter in interacting with consumers via email. The technologies, stages and tools of DM automation are systematized. It is proposed to collect all information about a particular consumer in a single user profile. This requires finding a technical solution for each business process and connecting software services into a single digital ecosystem. Collecting information about the company’s customers and their journey at all points of interaction in one place by integrating the services used into a single user profile allows you to make more effective decisions in marketing and business and achieve business goals

Anderson–Fabry Disease Homozygosity: Rare Case of Late-Onset Variant

Anderson–Fabry Disease (AFD) is a rare, X-linked lysosomal storage disorder caused by a mutation in the α-Galactosidase A gene resulting in α-Galactosidase A enzyme (α-Gal A) deficiency. The metabolic defect leads to the progressive accumulation of glycosphingolipids and the structural and functional impairment of affected organs. Due to the inheritance pattern, male patients are hemizygous with more severe manifestations of the disease as compared to females who, in most cases, are heterozygous with delayed and variable clinical presentation caused by uneven X-chromosome inactivation. Fabry disease cases are often identified by targeted screening programs in high-risk groups, such as in patients with end-stage renal disease, premature stroke, or unexplained cardiomyopathy. Here, we describe a unique case of a homozygous female patient identified by a nationwide screening program in hypertrophic cardiomyopathy patients. Before the systematic screening, the patient had a diagnosis of hypertrophic obstructive cardiomyopathy and was treated accordingly, including with alcohol septal ablation to reduce the obstructive gradient. The confirmation of Fabry disease led to the discovery of the same variant in several members of her family. The identified variant was c.644A>G, p.Asn215Ser (p.N215S), which is known to cause predominant cardiac involvement with late onset of the disease. This variant is amenable to oral therapy with the small-molecule chaperone migalastat, which was started and then interrupted due to the recurrence of the patient’s migraine and then re-initiated again after two years. During this period, the patient received enzyme replacement therapy with agalsidase beta but developed progressively worsening venous access. Our case illustrates the importance of the systematic screening of patients with clinical evidence of hypertrophic cardiomyopathy in whom the routine diagnostic process fails to discover Fabry disease, in particular variants with late-onset cardiac manifestations. Many of the late-onset variants are amenable to orally active therapy with migalastat, which significantly improves the comfort of the treatment. Its long-term results are being analyzed by a large international “Follow-me” registry, which was designed to verify the validity of pivotal trials with migalastat in Fabry disease.

Enriching the GEOFON seismic catalog with automatic energy magnitude estimations

Abstract. We present a seismic catalog (Bindi et al., 2024, https://doi.org/10.5880/GFZ.2.6.2023.010) including energy magnitude Me estimated from P waves recorded at teleseismic distances in the range 20°≤Δ≤98° and for depths shorter than 80 km. The catalog is built starting from the event catalog disseminated by GEOFON (GEOFOrschungsNetz), considering 6349 earthquakes with moment magnitude Mw≥5 occurring between 2011 and 2023. Magnitudes are computed using 1 031 396 freely available waveforms archived in EIDA (European Integrated Data Archive) and IRIS (Incorporated Research Institutions for Seismology) repositories, retrieved through the standard International Federation of Digital Seismograph Networks (FDSN) web services (https://www.fdsn.org/webservices/, last access: March 2024). A reduced, high-quality catalog for events with Mw≥5.8 and from which stations and events with only few recordings were removed forms the basis of a detailed analysis of the residuals of individual station measurements, which are decomposed into station- and event-specific terms and a term accounting for remaining variability. The derived Me values are compared to Mw computed by GEOFON and with the Me values calculated by IRIS. Software and tools developed for downloading and processing waveforms for bulk analysis and an add-on for SeisComP for real-time assessment of Me in a monitoring context are also provided alongside the catalog. The SeisComP add-on has been part of the GEOFON routine processing since December 2021 to compute and disseminate Me for major events via the existing services.

696 Optimal Management Strategy for Carotid Cavernous Aneurysm From Cost-effectiveness Perspective

INTRODUCTION: Most cavernous carotid aneurysms (CCAs) are asymptomatic benign lesions; therefore, they are frequently detected during routine clinical diagnostic processes. Most providers recommend routine surveillance instead of immediate management for CCAs given a natural history with a low risk of life-threatening complications. However, the cost-effectiveness of such care and the optimal follow-up interval remains unknown. METHODS: A decision analysis study was performed utilizing a Markov model with Monte Carlo simulations to simulate patients undergoing treatment or routine surveillance at different time intervals (half, 1-,2-,3-, 5-and 7-year intervals) for different size of CCAs (=<12 mm, 13- 24 mm, >=25 mm ). Input data for the model was extracted from the current literature. Probabilistic and deterministic sensitivity analyses were performed to evaluate the robustness of the Model. RESULTS: On base case analysis, following-up every 2 years with noninvasive imaging is the most cost-effective strategy for CCAs =< 12 mm, while annual follow up is cost-effective for large/giant (13-24 mm, >=25 mm) CCAs. The conclusions remain robust in probabilistic sensitivity and deterministic sensitivity analyses. As the risk of thrombo-embolic event gets higher and annual growth risk and annual rupture risk of CCAs increases, immediate treatment for large/giant (13- 24 mm, >=25 mm ) CCAs and annual follow up even prompt treatment for CCAs =< 12 mm is optimal. CONCLUSIONS: The most cost-effective management strategy for CCAs=< 12 mm and large/giant CCAs are following up every 2 years and following up every year, respectively. More frequent follow-up strategies or prompt preventive treatment for CCAs =< 12 mm and immediate treatment for large/giant CCAs would be more appropriate in patients with higher risk factors for cerebral ischemia, aneurysm growth and aneurysm rupture.

Vascular Endothelial Growth Factor Expression by Immunohistochemistry as a Possible Indicator of Prognosis in Invasive Breast Carcinoma of No Special Type.

Angiogenesis, the formation of new blood vessels from preexisting vascular network, is essential for tumor growth and spread. Vascular endothelial growth factor (VEGF) is a potent angiogenic growth factor. To assess the expression of VEGF in invasive carcinoma of no special type and its correlation with all the known prognostic factors of breast carcinoma. Descriptive. Mastectomy specimens were studied noting the clinical details. The formalin-fixed tissues were subjected to routine processing and hematoxylin and eosin sections and studied extensively for all the histological prognostic factors. Representative sections from each case with the tumor were subjected to immunohistochemistry (IHC) staining with VEGF, estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2/neu) antibodies. Descriptive statistics, Chi-square tests, contingency table analysis using SPSS for Windows. One hundred and twelve cases of invasive carcinoma of special type were studied to evaluate various clinicopathological parameters. The association of VEGF with clinicopathological parameters and all the known prognostic factors was studied to note its significance. VEGF overexpression was observed in 69% of the cases. It was noted that larger tumor size, higher histological grade, lymphovascular invasion, nodal involvement, tumor necrosis, high microvessel density, ER negativity, PR negativity, and HER2/neu positivity had a significant statistical association with VEGF overexpression. We conclude that incorporating VEGF as a biomarker along with the known factors into a prognostic index will not only help predict clinical outcome more accurately, but also determines the patient who can be benefited with combinational therapy including anti-VEGF factors.

Histopathological investigation and risk factors of Coccidiosis in camels (Camelus dromedarius), Algeria

Coccidiosis in dromedaries is an intestinal protozoan infection caused by apicomplexan parasites belonging to the genus Eimeria. A prospective examination was conducted at two slaughterhouses in southern Algeria. The primary objective of this investigation was to ascertain the prevalence of intestinal parasites in camels, elucidate the associated microscopic lesions, and identify the risk factors contributing to this infestation. In pursuit of this, we procured four segments of the intestines from 31 dromedarieas that appeared healthy and displayed no symptoms. Subsequently, these samples were collected, preserved, subjected to routine processing, and subsequently stained with haematoxylin and eosin (H&E). Intestinal parasitic infection showed an incidence rate of 45.16% (14 out of 31). Specifically, two types of parasites were discerned in the intestinal specimens through microscopic examination, namely Eimeria (41.93%; 13 out of 31) (p-value = 0.046) and Taenia (3.22%; 1 out of 31) (p = 0.001). Eimeria cameli was observed in the ceacum (41.93%; 13 out of 31), jejunum (12.90%; 4 out of 31), and in one instance in the duodenum (3.22%; 1 out of 31). Furthermore, numerous development stages of coccidia were identified, including gamonts, schizonts and oocysts. Deep microscopic lesions attributed to Eimeria cameli were detected, such as enteritis, eosinophilic infiltration and inflammation. In addition, associated risk factors were identified. This study has furnished valuable insights into parasitic infestations affecting dromedaries, particularly Eimeria cameli. The molecular studies are needed to delineate the diverse variations within Eimeria strains. Effective parasite control strategies specific to dromedary camels need to be developed.

Prospects for the use of artificial intelligence in civil justice

The article discusses the general prospects for the use of artificial intelligence technology in civil proceedings as a main and auxiliary tool at the current stage of the development of this technology. The main approaches to defining the concept of artificial intelligence were studied and its classification into weak, strong and superintelligence was characterized. It is emphasized that justice is usually administered by professional judges, as high demands are placed on their legal knowledge and life experience. It is argued that equal replacement of a judge in civil proceedings with artificial intelligence technology is currently impossible. The reasons for the impossibility of such a replacement are analyzed, namely the complexity and multifacetedness of controversial private-law relations, the impossibility of taking into account moral and ethical aspects, the need for flexibility in decision-making, the problem of the nature of the initial algorithms of artificial intelligence, the transparency (motivation) of decisions, the problem of determining the guilty party in court cases errors, data protection issue. It is noted that these reasons have a technological and legal basis. A critical assessment of the state’s conceptual approaches to the development of artificial intelligence in the field of justice is provided. The directions of the auxiliary application of artificial intelligence technologies in civil proceedings, which are reduced to the automation of routine tasks, processing and analysis of large volumes of data and evidence, and increasing the accessibility of justice, are considered. It has been proven that the use of artificial intelligence technology provides better opportunities for the participants in the judicial process to realize their rights and obligations, including the processing of large amounts of information. The importance of ensuring a high level of information protection when using artificial intelligence during the administration of justice in civil cases is emphasized. The need for regulatory regulation of the use of artificial intelligence technology in civil proceedings in combination with other spheres of public administration and branches of law is substantiated. It is indicated that the wider application of artificial intelligence in civil proceedings will require new technological solutions and revision of the legal foundations of the judicial branch of government.

Readiness of Local Educational Institution for ISO 9001:2015 Quality Management System Certification: A Comprehensive Analysis

Business certifications and accreditations are increasingly crucial for organizations, especially in educational institutions, to demonstrate service quality and gain a competitive edge. Quality management systems (QMS) will support these objectives and enhance product and service quality. This study specifically examines the readiness of local educational institution for ISO 9001 Quality Management System certification. It also evaluated the degree of impact among the respondents in terms of compliance with the QMS standard clauses. Additionally, the study identified the challenges faced by local educational institutions in obtaining ISO certification. The research design employed a descriptive approach and a survey questionnaire. Stratified random sampling was used to select a sample of 215 administration and academic personnel. The findings indicated that the assessment level for ISO 9001 is ready. However, among independent variables, leadership is the most important factor in demonstrating a high level of readiness for ISO certification. It emphasizes the importance of adhering to quality management requirements. Furthermore, the assessment differed when respondents were grouped according to their profiles. In addition, the respondents identified moderate issues related to resistance to change and a lack of proper organizational structure. Therefore, local educational institution must view certification as an ongoing commitment rather than a one-time project. They should establish routine processes to maintain the ISO standard, improve the institution’s image, and raise employees’ awareness of the QMS in their day-to-day operations.

Discipline, caregiving, and identity work of frontline professionals: Talking about the acts of compliance and resistance in the everyday practices of social workers

Abstract This article investigates how the identities of frontline professionals are (re)constructed in their talk about their everyday work activities. Based on a study of a mental health and addiction counselling service organization in Ontario, we illustrate that when talking about acting in accordance with their organizational policies, the social workers’ identities are disciplined by and appropriated from addressing the practices of documentation and regular meetings with their supervisors that constitute the routine processes of organizing. However, when discussing instances where they override the organizationally sanctioned rules, their identities are disciplined by the aspiration of fabricating a client-centred caregiver identity adopted from the dominant discourse in their profession. We, therefore, counterbalance the understanding that professionals’ identity work related to their deliberate micro-emancipation acts are merely an expression of agency and argue that their preferred resistant identities pertaining to their self-declared apparent deviation from the organizational order are also made within frameworks of disciplinary power. By delineating that both discursive conformity and resistance cut across the boundaries between acting in alignment with and against organizational guidelines, we unveil an underexplored complexity of conducting professional identity work associated with the interrelationships between practices of talk and action that has largely been overlooked in prior scholarship. We, therefore, offer an action-related analysis of discursive identity work that extends beyond the context of this study and informs future research.

The Effect of Breast Milk Odor on the Pain and Stress Levels of the Newborn During the Endotracheal Suction Procedure.

Objective: The study aimed to determine the effect of the breast milk odor on the pain and stress levels of the newborn during the endotracheal suction procedure. Method: The study was conducted in the randomized-controlled experimental design at the neonatal intensive care unit of the hospital in eastern Turkey between March 2022 and December 2023. The study population included newborns at the 37th to 41st gestational weeks who were receiving mechanical ventilation treatment in the neonatal intensive care unit of a hospital in eastern Turkey. All newborns who met the inclusion criteria during the data collection phase were included in the study. The study was completed with 88 newborns (44 in the intervention group and 44 in the control group). The Newborn Introductory Information Form, ALPS-Neo Newborn Pain and Stress Assessment Scale, and follow-up form were used to collect the study data. The breast milk odor of the mothers of the newborns was used as the intervention group in the study. The mothers of the newborns were contacted to obtain breast milk, and the information was obtained from the mother on the day of the procedure. One milliliter of breast milk was taken and dropped into a sterile sponge, and it was held 10 cm away from the baby's nose from 5 minutes before to 5 minutes after endotracheal suction. A routine endotracheal suction process was performed in the control group. Ethical principles were followed in the study. Results: We found that the intervention group's pain and stress score averages were lower than the control group during and after the endotracheal suction procedure (p < 0.05). Conclusion: We found that the breast milk odor reduced the pain, stress levels, and crying duration of newborns during the endotracheal suction process.

Expert consensus on clinical practice of EGFR exon20 insertion detection in non-small cell lung cancer in China (2024 edition)

About 82% of the Chinese lung cancer population is non-small cell lung cancer(NSCLC), and the epidermal growth factor receptor (EGFR) gene is the most common driver mutation in the Chinese lung cancer population (51.7%-54.4%). In recent years, the rapid development and wide application of targeted therapies, especially EGFR tyrosine kinase inhibitors, have significantly improved the survival time and quality of life of Chinese lung cancer patients. EGFR exon20 insertion (EGFR ex20ins) accounts for 0.3%-2.9% in NSCLC patients in China. Recently, several tyrosine kinase inhibitors have been approved for the therapy of EGFR ex20ins NSCLCs. The variety variants and high molecular heterogeneity of EGFR ex20ins NSCLCs asks for more accurate detection methods, and clinical routine detection process needs to be further standardized. On account to the practical questions of EGFR ex20ins detection, this consensus was finally reached based on the combination of literature, expert experience and internal discussion among committee members, in the hope of providing the guide for standardizing the EGFR ex20ins detection.

CONSTANS, a HUB for all seasons: How photoperiod pervades plant physiology regulatory circuits.

How does a plant detect the changing seasons and make important developmental decisions accordingly? How do they incorporate daylength information into their routine physiological processes? Photoperiodism, or the capacity to measure the daylength, is a crucial aspect of plant development that helps plants determine the best time of the year to make vital decisions, such as flowering. The protein CONSTANS (CO) constitutes the central regulator of this sensing mechanism, not only activating florigen production in the leaves but also participating in many physiological aspects in which seasonality is important. Recent discoveries place CO in the center of a gene network that can determine the length of the day and confer seasonal input to aspects of plant development and physiology as important as senescence, seed size, or circadian rhythms. In this review, we discuss the importance of CO protein structure, function, and evolutionary mechanisms that embryophytes have developed to incorporate annual information into their physiology.

Novel processing and staining methodology of bottlenose dolphin (Tursiops truncatus) teeth for age determination

ABSTRACT Age determination of bottlenose dolphins (Tursiops truncatus) is a critical tool in understanding both individual and population health. There are many methods of aging bottlenose dolphins including analysis of teeth, pectoral flipper radiographs, and epigenetics. The most common and oldest method for aging toothed cetaceans is the counting of growth layer groups (GLGs) in the teeth. Current techniques have technical and repeatability challenges. Therefore, a processing technique that results in better resolution of GLGs is needed. This study compares different decalcifications and different histochemical staining techniques. Decalcification was done using 10% EDTA, Kristensen’s decalcification, and Rapid Decalcification Solution (RDO). Following decalcification and routine processing, GLGs were assessed using Hematoxylin and Eosin (H&E), hematoxylin, Giemsa, Wright-Giemsa, Toluidine Blue (T-Blue), Masson’s Trichrome, and Congo Red staining techniques. Decalcification with Kristensen’s and staining with Masson’s Trichrome and Congo Red were determined to best highlight GLGs. This processing and staining was then applied to a sample population of 102 bottlenose dolphins that were evaluated independently and blindly by two observers. Of the 102 dolphin samples, 13 (12.7%) were unable to age due to no clear distinction or distortion between GLGs.

Morphological analysis of the digestive tract of Antillean manatee (Trichechus manatus manatus, Linnaeus, 1758) in northeast Brazil

Morphological studies of the digestive tract are relevant to know the biology and understand the eating habits to conserve species. Our objective was to analyze macroscopically and microscopically the digestive tract of Antillean manatee (Trichechus manatus manatus). Samples were collected from 20 animals found dead stranded on the coast of northeastern Brazil or that died during the rehabilitation process in captivity between July 1995 and March 2017. The macroscopic analysis of the gastrointestinal tract was carried out from the in loco monitoring of the two necropsies. For microscopic evaluation, tissue fragments were collected during necropsy, fixed with 10% buffered formaldehyde and subjected to routine histological processing, with 5 μm thick slices, and subsequently submitted to the Hematoxylin-Eosin staining method, the Verhoeff method, and the periodic acid-Schiff method. Macroscopically, neonates and adults have the same digestive tract structures, namely esophagus, stomach, duodenum, jejunum, ileum, cecum, colon, and rectum. The microscopic analysis demonstrated that the digestive tract is formed by four histological layers (mucosa, submucosa, muscularis, and serosa or adventitia). In the esophagus, esophageal glands are absent and there are elastic fibers in the submucosal layer and between the external muscle and adventitial layers. The stomach has a structure called the cardiac gland, which microscopically presents ontogenetic differences regarding the depth of the lining epithelium and the orderly distribution of cells along the gastric gland. In the duodenal ampulla and in the diverticula, duodenal glands were identified in the submucosa. The luminal portion of the mucous layer of the cecal ampulla, colon, and rectum is lined by keratinized stratified squamous epithelium. The digestive tract of the species Antillean manatee is similar to what has been reported for the order Sirenia.

Protocol: Prospective evaluation of feasibility, added value and satisfaction of remote digital self-assessment for mild cognitive impairment in routine care with the neotivCare app

IntroductionTimely diagnosis of mild cognitive impairment (MCI) in Alzheimer’s disease is crucial for early interventions, but its implementation is often challenging due to the complexity and time burden of required...