Ability Of Roots Research Articles

Reciprocation and rotation nickel-titanium file systems’ cyclic fatigue and centering ability in premolars accessed by ultraconservative and traditional access cavities: An in vitro study

Introduction: With the importance of restricted access cavities, this study aimed to compare the cyclic fatigue and the centering ability of TruNatomy rotary file versus R-motion reciprocating file systems in premolars accessed by traditional versus ultraconservative access cavity preparations. Materials and Methods: Forty extracted intact maxillary first premolars were divided into two groups (n = 20/group) according to the access cavity design (traditional or ultraconservative). Each group was then subdivided according to the type of file used for instrumentation (TruNatomy [26/0.04] or R-motion [30/0.04] [n = 10 teeth/subgroup]). Cone-beam computed tomography (CBCT) scans were obtained for all the teeth before and after instrumentation. The teeth were accessed and instrumented by three in-and-out pecking motions repeated two times until the instrument reached the working length. Every instrument was only used to prepare one canal. For cyclic fatigue assessment, the used files, in addition to 10 brand-new TruNatomy and R-motion files that were used as control groups, were tested in stainless-steel artificial canals, and the time to fracture was used to determine the number of cycles to fracture (NCF). After obtaining and analyzing the before and after instrumentation CBCT images, the centering ability of the buccal roots was calculated at 3, 5, and 7 mm from the apex. Data were statistically analyzed. Results: There were no differences in NCF and centering ability of each file in both access cavities (P > 0.05). There were higher NCF values of the R-motion file compared to the TruNatomy file (P < 0.05). The R-motion file showed significantly higher centering than the TruNatomy file in the traditional access cavity at the 3 and 5 mm levels and the 3 mm level in the ultraconservative access cavity (P < 0.05). Conclusion: The R-motion file had significantly higher cyclic fatigue resistance and centering ability than the TruNatomy file. The ultraconservative access cavity had no unfavorable influence on the cyclic fatigue resistance and the centering ability of R-motion and TruNatomy files.

ВЛИЯНИЕ ТЕХНОЛОГИИ ПРОИЗВОДСТВА МАТОЧНЫХ РАСТЕНИЙ ВИНОГРАДА НА ИХ СПОСОБНОСТЬ К ВЕГЕТАТИВНОМУ РАЗМНОЖЕНИЮ

For many years, grapes were considered to be an unpromising crop in the conditions of the Central Non-Black Earth Zone but their wider spread has been encouraged by the appear- ance of new varieties whose berries ripen in a relatively short time. The assortment of modern grape varieties for amateur cultivation in the non-Black Earth Zone consists mainly of interspecific hybrids based on Vitis amurensis Rupr., Vitis riparia Michx., Vitis labrusca L., Vitis berlandieri Planch., which causes problems with their vegetative propagation. The article is devoted to the study of the effect of the method of obtaining planting material from vine mother plants on the increase of the regenerative capacity of the singletree cuttings obtained from them. During the threeyear research on the grape varieties Kishmish No. 342 and Moskovskiy Beliy, experiments were carried out on the additional growth of mother plants propagated by green and singletree cuttings and by the method of clonal micropropagation. As a result of the research, the advantage of using the technology of clonal micropropagation in the production of seedlings for the establishment of vine mother plantations was revealed. At the same time, the variety Kishmish No. 342 showed reliable differences from the control in terms of development indicators from the 2nd year of cultivation, and the variety Moskovskiy Beliy – from the 3rd year. Both varieties received 1.8–2.1 times more standard seedlings from mother plants propagated in vitro for three years on the basis of high rooting ability of singletree cuttings and the proportion of seedlings according to GOST 31783–2012.

Own-rooted Walnut Propagule of Four Walnut (Juglans) Rootstocks and Main Cultivated Cultivar Liaoning 1 Acquirement through Layering under Field Conditions

Walnut, a woody plant, is regarded as having difficulty rooting when propagated by vegetative methods, such as cutting and layering. A layering experiment was conducted in 2018 and 2022. In 2018, some Juglans species, including J. regia L. seedling (JR), J. regia cv. Liaoning 1 (JR LN1), J. hopeinesis Hu seedling (JH), J. mandshurica Maxim seedling (JM), and J. nigra L. seedling (JN), were the mother plants. The specific research hypotheses were that own-rooted walnut propagule could be obtained through layering. the rooting capacity of different Juglans species would be different, and the rooting ability of JN would be the highest among the samplings. The results indicated that all of these species in the experiment could be rooted by etiolation and indole-3-butyric acid (IBA) treatment and that root occurrence was found 6 to 7 weeks after IBA treatment. The layers (shoots from the mother plant) on the seedlings of JR, JH, and JM obtained rooting percentages (RP) of 75.55%, 84.45%, and 86.67%, respectively, and root numbers (RNs) of 21.8, 42.8, and 38.8, respectively, after 20 days of etiolation and 1% IBA treatment. JR LN1 had difficulty rooting in equal conditions and had a RP of 31.11%. In 2022, JR LN1 was the only mother plant and the IBA concentration was increased to obtain satisfactory RP and RN. With the 4% and 8% IBA treatments, RPs of 88.9% and 93.3% and RNs of 40.3 and 27.7, respectively, were achieved. During the experiment, the RP, RN, root length (RL), and root diameter (RD), as well as the layer height (LH) and layer diameter (LD), were investigated and evaluated. Layers with low vigor were more likely to root, as shown by a nonparametric test conducted for the height and diameter of the layers of the rooting and nonrooting groups. A significantly negative correlation (r = −0.548) was observed between RN and LH. Moreover, the quality of the best results of JR LN1 layering propagule and that with ‘liaoning 1’ 1-year-old seedling were compared. Our results provide more support for the possibility of vegetative propagation of walnut by layering and more information regarding the clonal cultivation of walnut trees and the own-rooted seedling establishment of walnut cultivars.

Влияние стимулятора роста растений (янтарной кислоты), сроков черенкования на укореняемость зеленых черенков груши и айвы

The article highlights the research results on the rooting of green pear and quince cuttings in the first and second decades of June. Green cuttings were taken from mother shrubs and treated with the plant growth stimulator of succinic acid. As the pear and quince cuttings took roots, we determined the rooting ability of rootstocks, number of standard rootstocks, height of the rooted rootstocks, diameter of the conditional root neck, number of roots, length of the root system. The rootstocks of pear PG 12 (k), PG 2, PG 17-16, PG 333 and quince VA 29, Severnaya demonstrated the best indicators of rooting ability, number of standard rootstocks, diameter of the conditional root neck, number and length of roots.

MWCNTs Alleviated saline-alkali stress by optimizing photosynthesis and sucrose metabolism in rice seedling

ABSTRACTSaline and alkali stress affects the growth and development, survival rate, and final yield of rice, while new nano materials can have a positive effect on rice growth. In order to investing the effects of carboxymethyl multi walled carbon nanotubes (MWCNTs) on the growth and development of rice seedlings under salt alkali stress, rice seedlings were cultured using rice variety “Songjing 3” using nutrient solution water culture method. The effects of MWCNTs on water absorption capacity, leaf photosynthesis, and sucrose metabolism of rice seedlings under 50 mmol/L saline-alkali stress (1NaCl: 9Na2SO4: 9NaHCO3: 1Na2CO3) conditions were investigated. The results showed that MWCNTs can improve the water use ability of roots and leaves, especially the water absorption ability of roots, which provides a guarantee for the improvement of rice biomass and the enhancement of leaf photosynthetic capacity under adverse conditions. After treatment with MWCNTs, the photosynthetic rate (Pn), stomatal conductance (gs), and transpiration rate (Tr) of leaves increased significantly, and the photochemical quenching value (qP), photochemical quantum efficiency value (Fv/Fm), and electron transfer rate value (ETR) of chlorophyll fluorescence parameters increased significantly, which is beneficial to the improvement of the PSII photosynthetic system. MWCNTs treatment promoted the increase of photosynthetic pigment content in leaves under salt and alkali stress, improved the ratio of Chla and Chlb parameters, increased the activities of key photosynthetic enzymes (RUBPCase and PEPCase) in leaves, increased the value of total lutein cycle pool (VAZ), and significantly enhanced the deepoxidation effect of lutein cycle (DEPS), which can effectively alleviate the stomatal and non stomatal constraints on leaf photosynthesis caused by salt and alkali stress. MWCNTs treatment significantly enhanced the activities of sucrose phosphate synthase (SPS) and sucrose synthase (SS) under salt and alkali stress, and decreased the activities of soluble acid invertase (SAInv) and alkaline/neutral invertase (A/N-Inv), indicating that MWCNTs promoted sucrose synthesis while inhibiting sucrose decomposition, thereby promoting sucrose accumulation in rice leaves. This study can provide theoretical and experimental basis for the application of MWCNTs to the production of rice under salt and alkali stress, and can find a new way for rice production in saline and alkaline lands.

Identification of marine debris at Avicennia mangrove root in Pangandaran Beach, West Java

This research was carried out in Pangandaran Regency, specifically in the Batukaras, Nusawiru, and Bojongsalawe areas, in March–April 2022. This research to determine the characteristics of Avicennia type mangroves, habitat characteristics, and the ability of Avicennia type mangrove roots to trap debris. The method used is the Line Transect (LT) method. The results obtained from research on the characteristics of this type of Avicennia mangrove include that the circumference of mangrove roots ranges from 2–24 cm, the height of mangrove roots ranges from 3-83 cm, and the number of mangrove roots ranges from 925–2988 roots. The wider the area, the more debris you will find. Based on the results of the calculation of the characteristics of the Avicennia-type mangrove habitat in Pangandaran, it is in a good range. The calculations include salinity in the range of 15–22 ppt, a temperature of 32–34 °C, a pH value of 7,6–8, and DO (dissolved oxygen) of 4–4,5 mg/L. The trapping of waste in the Avicennia root area is categorized as strong. So the denser the mangrove roots are, the density of debris will increase. This is proven by the large amount of debris found in Batukaras.Keywords: Avicennia mangrove; Characteristics, Category, Density, Marine debris; Type.

The Impact of Dates and IBA Treatment on the Root Ability of Semi-Hard Wood Cuttings of Three Olive (Olea europaea L.) Cultivars

The University of Mosul in Iraq conducted the experiment in a plastic greenhouse run by the Department of Horticulture and Landscape Design at the College of Agriculture and Forestry. For 1/2/2022 – 1/6/2022. To find out what happens to semi-hardwood cuttings of three olive varieties Hojiblanca, Picual, and Arbequina—when they are taken five times a month from February to June and then soaked for 5 seconds in three concentrations of IBA solution (0, 3000, and 5000 mg l-1) to see how well they root and grow. The cuts that were taken on March 1 had the most leaves, the most leaf area, and the longest roots per cutting. Cuttings that were soaked in 3000 mg l-1 and 5000 mg l-1 took root faster and had longer roots. Soaking in 5000 mg L-1 increased the leaf area of rooted cutting. Picual and Arbequina cuttings rooted less often and had shorter roots than Hojiblanca cuttings. In terms of longevity and leaf count, Hojiblanca cuttings did better than Arbequina cuttings. Cuttings of Hojiblanca and Picual had more leaves, cuttings that had grown roots, and roots than cuttings of Arbequina. However, Hojiblanca cultivar collected on March 1st treated with 5000 mg.l-1 gave 60.00%, 27.80 leaf cutting-1, 1.32 cm2, 36.51 cm2 cutting-1, 14.27cm. Hojiblanca cultivar cuttings treated with 3000 mg.l-1 had 11.61 root cutting-1. The Picual olive control treatment had the highest survival rate (63.33%) on March 1.

Research on Ecological Effects of Microplastics and Removal Methods

In recent years, with the continuous accumulation of microplastics (MPs), the environmental pollution caused by MPs has become increasingly serious. In this paper, whether the accumulation of MPs will cause serious damage to the ecological environment and organisms and if harm is caused, how to eliminate it are analyzed. The results show that MPs are mainly produced by human activities, which have caused pollution to the water environment, soil environment and atmospheric environment. MPs destroying the water cycle, enriching heavy metals in the soil and aggravating the greenhouse effect indirectly threaten the organisms living in the environment. Due to the large amount of MPs enrichment, the survival rate of soil animals and aquatic animals is decreasing year by year, their growth and development are seriously inhibited, and the living conditions are becoming difficult. Because of the blockage of MPs, the ability of plant roots to absorb water is greatly reducing, the activity of various enzymes in the plant body is decreasing, and the efficiency of photosynthesis is affected. As the highest trophic level, humans are naturally not immune to the harm of MPs. The higher the trophic level, the more enriched the content of MPs, which poses a great threat to health. In the future, the elimination of MPs will become a focus of attention. The existing physical treatment method, chemical treatment method and biological treatment method should be improved and reasonably combined to achieve the optimal treatment effect, hoping to reduce the harm of MPs as much as possible.

Comparative analysis of anatomical characteristics and phenolic compounds of two highbush blueberry (Vaccinium corymbosum L.) cultivars with different rooting ability of semi-hardwood cuttings

There is no information on morphological, anatomical or phenolic concentration differences between blueberry cultivars that could be related to the differential rooting responses. In the present work, such differences were evaluated comparing the rooting zones of cuttings from two blueberry cultivars (‘Jewel’ and ‘Violeta’ with 4n and 2n ploidy level, respectively). The parameters were measured during 150 days of rooting (rooting day after cutting insertion; RAI) at 30-day interval after treatment with 3 g/L of IBA. Stem length, stem diameter and number of leaves and buds were measured and tissue sections from cuttings were stained to measure tissue thickness. Also, the total phenolic content (TPC) by Folin-Ciocalteu method and the phenolic profile by liquid chromatography coupled to a diode array detection were also evaluated in rooting zones. ‘Jewel’ rooted earlier and in higher percentage than 'Violeta'. ANOVA showed that phenolic compounds, in a higher extent, and some morphological and anatomical parameters, in a lower extent, had a positive effect on rooting capacity. In both blueberry cultivars, the cuttings with the highest phenolic content presented an early rooting capacity. The multivariate analyses allowed us to corroborate these results. FiPLS selected common variables (coumaric acid and stem diameter) and specific variables depending on the cultivar (gallic acid, stem length and number of leaves for ‘Jewel’; and, vanillic acid, rutin, and cortex thickness for ‘Violeta’). PCA allowed to group the ‘Violeta’ samples (unrooted and rooted at 60, 90, 120 and 150 RAI) in compact homogeneous clusters with an adequate explanation rate (44.7 %). From all parameters, stem diameter seems to be inversely related, and cortex thickness and p-coumaric acid and rutin seems to be positively related to the earliest rooting day after insertion (RAI 60), while the largest the stem, the earlier the rooting. In future works, more cultivars and other parameters and phenolic compounds that can induce the rooting in cuttings, including their evolution over time, should be investigated.

The Impact of Vegetation Roots on Shallow Stability of Expansive Soil Slope under Rainfall Conditions

The impact of reinforcing vegetation roots on the stability of expansive soil slopes with moisture absorption and expansion was investigated. Then, poinsettia is selected as the slope protection plant, and ABAQUS software (version 2022) with secondary development is used to simulate the moisture absorption and expansion of the expansive soil slope. After that, the strength reduction method is employed to study the effects on the displacement and plastic zone, and on the shallow layer of the expansive soil slope at different rainfall conditions. The following points are revealed: (1) The roots of the poinsettia can reduce the displacement of the slope. But, when the rainfall intensity exceeds the soil permeability coefficient, the soil reinforcement effect decreases. (2) The poinsettia root system can alleviate the concentration of plastic strain, disperse the plastic zone, and increase slope stability along the distribution of the roots. (3) The poinsettia roots can improve the shallow stability of the slope. But when the rainfall intensity exceeds the surface permeability coefficient, the magnitude of the reinforcement decreases. The results demonstrate that the poinsettia roots can enhance shallow slope stability. However, with increasing rainfall intensity, the ability of the poinsettia roots to enhance shallow slope stability gradually weakens.

Silicon improves root system and canopy physiology in wheat under drought stress

AimsRoot system is an important regulator for unevenly distributed below-ground resource acquisition. In a rainfed cropping environment, drought stress (DS) significantly restricts root growth and moisture uptake capacity. The fact that silicon (Si) alleviates DS in wheat is widely reported, but its effects on the wheat root system remain unclear.MethodsThe present study investigated the effect of pre-sowing Si treatment on two contrasting wheat cultivars (RAC875, drought-tolerant; Kukri, drought-susceptible) at early growth stages. The cultivars were grown in a glasshouse in a complete randomized design with four replications and two watering treatments. Various root traits and physiological data, including non-destructive infrared thermal imaging for water stress indices, were recorded.ResultsUnder DS and Si (DSSi), Kukri had a significant increase in primary root length (PRL,44%) and lateral root length (LRL,28.1%) compared with RAC875 having a substantial increase in PRL (35.2%), but non-significant in LRL. The Si-induced improvement in the root system positively impacted canopy physiology and significantly enhanced photosynthesis, stomatal conductance and transpiration in Kukri and RAC875 under DSSi. Canopy temperature was reduced significantly in Kukri (4.24%) and RAC875 (6.15%) under DSSi, while canopy temperature depression was enhanced significantly in both the cultivars (Kukri,78.6%; RAC875, 58.6%) under DSSi.ConclusionThese results showed that Si has the potential to influence below-ground traits, which regulate the moisture uptake ability of roots for cooler canopy and improved photosynthesis under DS. It also suggests a future direction to investigate the underlying mechanisms involved in wheat’s Si-induced root growth and moisture uptake ability.

Improving rice nitrogen-use efficiency by modulating a novel monouniquitination machinery for optimal root plasticity response to nitrogen.

Plant nitrogen (N)-use efficiency (NUE) is largely determined by the ability of root to take up external N sources, whose availability and distribution in turn trigger the modification of root system architecture (RSA) for N foraging. Therefore, improving N-responsive reshaping of RSA for optimal N absorption is a major target for developing crops with high NUE. In this study, we identified RNR10 (REGULATOR OF N-RESPONSIVE RSA ON CHROMOSOME 10) as the causal gene that underlies the significantly different root developmental plasticity in response to changes in N level exhibited by the indica (Xian) and japonica (Geng) subspecies of rice. RNR10 encodes an F-box protein that interacts with a negative regulator of auxin biosynthesis, DNR1 (DULL NITROGEN RESPONSE1). Interestingly, RNR10 monoubiquitinates DNR1 and inhibits its degradation, thus antagonizing auxin accumulation, which results in reduced root responsivity to N and nitrate (NO3-) uptake. Therefore, modulating the RNR10-DNR1-auxin module provides a novel strategy for coordinating a desirable RSA and enhanced N acquisition for future sustainable agriculture.

Delayed rooting hormone application and stem cutting collection time improved Flacourtia indica rooting ability and root growth

Flacourtia indica (Burm.f.) Merr. is an important fruit and medicinal tree in Southern Africa. Efforts to achieve early fruiting have failed with juvenile propagules (planting materials) such as seed. Assessing rooting ability of mature (fruit bearing) F. indica stem cuttings is vital to attaining early fruiting, or precocious fruiting, which reduces the long juvenile phase experienced by many woody fruit trees when propagated from seeds (juvenile planting materials). The objectives of this study were to assess responses of mature stem cuttings to different application times of rooting hormone (Seradix® No. 2) and collection times of stem cuttings, and also to identify important pheno-phases in the yearly tree growth cycle of F. indica. A significantly higher rooting percentage (p < 0.001) was obtained with delayed Seradix® No. 2 application than with immediate application. Stem cuttings collected at flowering and leaf fall seasons were responsive to Seradix® No. 2 application, but all stem cuttings collected at fruiting time failed to produce roots. For stem cuttings collected during flowering time, immediate Seradix® application resulted in significantly (p = 0.0482) more roots than in stem cuttings subjected to delayed Seradix® application. The failure to produce roots of cuttings collected at fruiting time could be due to low sugars (carbohydrates) in the stem cuttings. The study concluded that the best time to collect stem cuttings from mature F. indica trees for rooting is either at flowering or leaf fall stages and that delayed Seradix® No. 2 application improves rooting of stem cuttings from mature F. indica trees.

Antigravitropic PIN polarization maintains non-vertical growth in lateral roots

Lateral roots are typically maintained at non-vertical angles with respect to gravity. These gravitropic setpoint angles are intriguing because their maintenance requires that roots are able to effect growth response both with and against the gravity vector, a phenomenon previously attributed to gravitropism acting against an antigravitropic offset mechanism. Here we show how the components mediating gravitropism in the vertical primary root—PINs and phosphatases acting upon them—are reconfigured in their regulation such that lateral root growth at a range of angles can be maintained. We show that the ability of Arabidopsis lateral roots to bend both downward and upward requires the generation of auxin asymmetries and is driven by angle-dependent variation in downward gravitropic auxin flux acting against angle-independent upward, antigravitropic flux. Further, we demonstrate a symmetry in auxin distribution in lateral roots at gravitropic setpoint angle that can be traced back to a net, balanced polarization of PIN3 and PIN7 auxin transporters in the columella. These auxin fluxes are shifted by altering PIN protein phosphoregulation in the columella, either by introducing PIN3 phosphovariant versions or via manipulation of levels of the phosphatase subunit PP2A/RCN1. Finally, we show that auxin, in addition to driving lateral root directional growth, acts within the lateral root columella to induce more vertical growth by increasing RCN1 levels, causing a downward shift in PIN3 localization, thereby diminishing the magnitude of the upward, antigravitropic auxin flux.

Elevated CO2 Influences the Growth, Root Morphology, and Leaf Photosynthesis of Cacao (Theobroma cacao L.) Seedlings

Growing quality seedlings is a challenge for sustainable cacao production as the survival rate of young seedlings is strongly influenced by environmental factors that affect the productivity of cacao farmers. In this study, cacao (Theobroma cacao L.) seedlings were cultivated in a nursery, and the effects of elevated CO2 concentrations (approximately 800 ppm) applied to cacao seedlings during daytime (6:00–17:59) on the root growth, morphology, and leaf photosynthetic capacity were examined. Treatment with elevated CO2 significantly improved root growth, dry matter weight, and root/shoot ratio. Three-dimensional imaging of roots showed that lateral roots grew longer horizontally, lateral roots and fine roots were distributed over a larger area, and root surface and root volume increased significantly under elevated CO2 treatment. Accurate quantification of root morphology using X-ray CT indicated that the treatment with elevated CO2 concentrations may significantly affect root quality during the seedling stage by expanding the distribution range of lateral and fine roots, which increases the ability of lateral roots to elongate and absorb water and nutrients from the superficial layers. The photosynthetic characteristics of the aboveground leaves of cacao seedlings exposed to elevated CO2 concentrations showed a tendency to adapt to elevated CO2 concentrations by increasing light-use efficiency and CO2-use efficiency. Therefore, the treatment of cacao seedlings with elevated CO2 concentrations improved the growth quality of seedlings due to the characteristics of the roots as large sinks.

Genetic Assessment, Propagation and Chemical Analysis of Flowers of Rosa damascena Mill. Genotypes Cultivated in Greece

Rosa damascena Mill. is commercially the most important rose species used to produce essential oils. The plants of this species, cultivated in the district of Western Macedonia (Greece) for rose oil production, originated from indigenous genotypes but also nurseries abroad, mainly from Bulgaria. The present study investigated the genetic relationship between nine genotypes of R. damascena from Greece, one genotype from Turkey, three genotypes from Bulgaria and three genotypes from France using the molecular markers ISSR and SCoT. Also, the rooting ability of shoot cuttings from these nine genotypes was investigated by applying 2 g/L of the rooting regulator K-IBA. In addition, petals were chemically analyzed using GC-MS and LC-MS to identify the compounds that are the main components of the rose oil. The nine rose genotypes of R. damascena, cultivated in Greece, one from Turkey and one of the three genotypes from Bulgaria were clustered in one clade in the dendrogram. The other two genotypes from Bulgaria were clustered in a separate clade that demonstrated the existence of genetic diversity among the three Bulgarian genotypes, while the French genotypes were clustered in a third clade. The shoot cuttings rooted relatively easily (55–70%) with the application of K-IBA, without any significant differences among the nine genotypes. Large variation was observed among the nine genotypes in the main volatile compounds of the flower petal extracts, which are related to rose oil components. For these compounds, the concentrations in μg/g of the fresh petal weight were 2-phenylethylalcohol (1148.35–2777.19), nerol (27.45–64.93), citronellol (88.45–206.59), geraniol (69.12–170.99) and nonadecane (209.27–533.15). Of the non-volatile compounds, gallic acid was the most abundant phenolic acid in the petal extracts of the nine genotypes (0.28–0.82 μg/g), while for the flavonoids, quercetin and kaempferol variations of 0.35–1.17 μg/g and 0.26–2.13 μg/g were recorded, respectively.

GWAS of adventitious root formation in roses identifies a putative phosphoinositide phosphatase (SAC9) for marker-assisted selection.

Rose propagation by cuttings is limited by substantial genotypic differences in adventitious root formation. To identify possible genetic factors causing these differences and to develop a marker for marker-assisted selection for high rooting ability, we phenotyped 95 cut and 95 garden rose genotypes in a hydroponic rooting system over 6 weeks. Data on rooting percentage after 3 to 6 weeks, root number, and root fresh mass were highly variable among genotypes and used in association mappings performed on genotypic information from the WagRhSNP 68 K Axiom SNP array for roses. GWAS analyses revealed only one significantly associated SNP for rooting percentage after 3 weeks. Nevertheless, prominent genomic regions/peaks were observed and further analysed for rooting percentage after 6 weeks, root number and root fresh mass. Some of the SNPs in these peak regions were associated with large effects on adventitious root formation traits. Very prominent were ten SNPs, which were all located in a putative phosphoinositide phosphatase SAC9 on chromosome 2 and showed very high effects on rooting percentage after 6 weeks of more than 40% difference between nulliplex and quadruplex genotypes. SAC9 was reported to be involved in the regulation of endocytosis and in combination with other members of the SAC gene family to regulate the translocation of auxin-efflux PIN proteins via the dephosphorylation of phosphoinositides. For one SNP within SAC9, a KASP marker was successfully derived and used to select genotypes with a homozygous allele configuration. Phenotyping these homozygous genotypes for adventitious root formation verified the SNP allele dosage effect on rooting. Hence, the presented KASP derived from a SNP located in SAC9 can be used for marker-assisted selection in breeding programs for high rooting ability in the future.

Investigation of the Role of Brassica Roots &amp; Fusarium Mycelium in Accumulation of Gold Particles

Abstract In the last decade, the production and use of metal particles in the nanoscale spread are due to the latter efficiency in solving many obstacles in various fields of science, engineering, agriculture and medicine. Gold particles in the small scale show catalytic, magnetic, electrical, mechanical, biological and chemical properties due to the high surface-to-volume ratio where appearance of these particles interact and move higher in properties and strength. Several studies have obtained nano-particles of several types of metals such as silver, copper and gold from plants and microorganisms. The current study, thus, aimed at finding out the ability of the mustard plant root to collect gold particles(Au), as well as the mycelium of the fungus Fusarium oxysporum, where the examination technique using the scanning electron microscope (SEM) was used to confirm the presence and accumulation of gold particles. It is vital using the biological system of the organism. This method is environmentally and friendlydoes not result in toxins or high use of energy. It is characterized by its low cost and high efficiency in obtaining results. Three different concentrations of colloidal gold solution were used in the current study 2, 4, 6 ml, which produced a concentration exceeding 6 ml. In deposition of the largest percentage of gold in each of the plant root and fungal hyphae.

The transcription factor PpTGA9 improves salt tolerance by interacting with PpATP1 in peach

Salt stress hinders plant growth and development by affecting the ability of plant roots to absorb water, in turn affecting photosynthetic capacity and other metabolic processes, and ultimately reducing yield and quality. Peach (Prunus persica) is a salt-sensitive species, but the mechanism of peach adaptation to salt stress is not clear. We previously screened 50 basic leucine zipper (bZIP) transcription factors (TFs) in peach and found that the PpbZIP30 TF has several conserved functions. The gene encoding this TF was found to be highly homologous (75.19 % similarity) to Arabidopsis thaliana AtTGA9, so it was named PpTGA9. Expression of PpTGA9 was induced by exogenous application of NaCl after different treatments of exogenous signals in peach seedlings, and overexpressed PpTGA9 in Arabidopsis plants enhanced resistance to salt stress. To clarify the role of PpbZIP30/PpTGA9 in salt resistance, PpATP1 was screened using a yeast two-hybrid cross and further validated by bimolecular fluorescence complementation (BiFC), pull down, and subcellular localization to show that the two interacted with each other in vitro and in the nucleus. To investigate the mechanism of interaction between PpTGA9 and PpATP1, we divided PpTGA9 into five segments and cotransformed them with PpATP1. We found that two glutamic acid (Gln)-rich structural domains (Q1 and Q2) were essential in the interaction between PpTGA9 and PpATP1, however, the N and bZIP regions did not have significant roles in this interaction. Further study revealed that PpATP1 protein interacts with itself to form homodimers and PpATP1 expression specificity analysis showed that it can rapidly respond to salt stress signals. These findings suggest that PpATP1 interacts with two Gln-rich structural domains (Q1 and Q2) of PpTGA9 and simultaneously forms homodimers. The two also form a PpTGA9/PpATP1 complex that has an active role in improving salt tolerance in peach.

Growth and yield of rice from mycorrhizal enrichment seedlings on different soil water content

The growth and yield of rice in limited soil water are expected to increase through the application of mycorrhizae due to the increasing root ability to absorb water and nutrients from the soil. The study aimed to determine the role of mycorrhizae on the growth and yield of rice plants in different soil water content. The study was conducted in Mempawah District, West Kalimantan, in February-June 2022. The experiment was arranged in a split-plot randomized complete block design with main plots of mycorrhizae treatment on seedlings (without and with mycorrhizae application), and subplots of soil water content (100%, 80%, 60%). The results showed that mycorrhizae could colonize 60-80% of roots and increase phosphorus absorption at up to 60% of soil water content. Application of mycorrhizae improved plant growth up to 80% of soil water content for the maximum number of a tiller, and 60% of soil water content for the leaf area. Mycorrhizae performed better to increase yield at up to 80% soil water content on the number of productive tillers and grain index of 1,000 seeds, and it performed better at a soil water content of 100% for grain weight per hill.&#x0D; Keywords: microorganisms, plant resistance, rice production, root colonization