Root Parts Research Articles

Effects of Sulfamethazine in Soil on Rice Growth

For the sake of investigating the effects of residual antibiotics in soil on plant growth, sulfamethazine, which is commonly detected in soil, was selected in this project. In general, the growth index of rice at the seedling and mature stages, physiological/biochemical characteristics of roots and leaves, antibiotic residues, enrichment factors, and transport coefficients in various rice organs were respectively tested and analyzed to evaluate the ecological effects of sulfamethazine residues on rice. The results revealed that the inhibitory effect of sulfamethazine on plant height and biomass was maintained during the whole growth cycle. Moreover, the effect at the seedling stage was greater than that at the growth maturity stage, and the root part was more easily influenced than the seedling section. The root activity, nitrate reductase activity, and leaf chlorophyll content at the seedling stage were hindered by the increase in antibiotic content. By contrast, the antioxidant enzyme change showed a different tendency, in which the superoxide was activated, and the catalase and peroxidase were firstly activated and then inhibited. The sulfamethazine accumulation in various rice organs was in the order of root>leaf>sti>grain. The results of antibiotic risk assessment of rice grains exhibited that EDI/ADI was less than 0.1, indicating no health risk. The effect of sulfamethazine on enrichment factors and transport coefficients at the growth maturity stage was more obvious than that at the seedling stage. Considering the adverse effects of sulfamethazine on rice, we need to take the ecological effects of sulfamethazine on plants into consideration when applying livestock manure as organic fertilizer or using aquaculture water for irrigation, to ensure crop production safety.

HPLC Analysis of Polyphenolic Compounds in Lysimachia nummularia L. and Comparative Determination of Antioxidant Capacity

Lysimachia nummularia L. is a perennial herbaceous plant rich in bioactive compounds, which can be utilized for medicinal purposes. The present work aims to analyze the phenolic compounds from different parts of the Lysimachia nummularia L. plant using the HPLC technique: Lysimachiae radix (the root part), Lysimachiae herba (the aerial part), Lysimachiae flores (flowers). In order to determine the phenolic compounds, extraction from the three categories of vegetable products was performed with ethanol 70% (v/v) using three extraction methods: (i) Soxhlet extraction, (ii) maceration and (iii) ultrasonic-assisted extraction. The content of polyphenols was determined by the Folin–Ciocalteu method, and the antioxidant activity was evaluated by the DPPH, ABTS, FRAP and CUPRAC methods. The antioxidant activity was correlated with the content of phenolic compounds in the analyzed extracts. The following phenolic compounds were separated, identified and quantified: 3-O-methylgallic, gallic, ferulic, caffeic, chlorogenic, p-coumaric acids and trans-resveratrol. According to the experimental data, the highest content of total polyphenols was observed in the hydroethanolic extract from Lysimachiae flores (22.10 ± 1.48 mg gallic acid/g), which also presented remarkable antioxidant activity.

Potential of roots and shoots of Napier grass for arresting soil erosion and runoff of mollisols soils of Himalayas

In this study, a soil filled Hydraulic Tilting Flume (HTF) was used as a test plot under simulated rainfall conditions. This flume was filled with mollisols soils (sandy loam in texture) collected from tarai region of Himalayas. The effects of root and shoot characteristics of Napier grass in terms of leaf area index (LAI), shoot length (SL), number of leaves (NL), number of tillers (NT), shoot biomass (SB), root density (RD), root length (RL), root biomass (RB), and total biomass (TB) were investigated on runoff and sediment outflow at 90, 120 and 150 days after planting (DAP). Four simulated rainfall intensities namely 4.0, 6.5, 8.3 and 9.4 cm/h over three land slopes of 1, 2 and 3% were selected. Runoff samples collected from whole plant plot and only root plot were analyzed for runoff and sediment outflow. Findings revealed that Napier grasses were very effective to reduce runoff and sediment outflow and its efficacy increased with the extended growth stages. The reduction in runoff and sediment outflow at 90, 120 and 150 DAP was obtained as 56% and 85%, 68% and 90%, and 74% and 96%, respectively, as compared to bare plot conditions. It was observed that the comparative contribution of shoots in runoff rate reduction was higher than the roots. On the contrary, the root part of the plant showed more contribution in sediment rate reduction as compared to the shoot part. Step wise regression was attempted for the selection of effective input parameters to establish authentic runoff and sediment outflow models. Power form of multiple non-linear regression (MNLR) showed very satisfactory results for predicting runoff and sediment outflow with coefficient of determination (R2) as 97.4% and 99.0%, respectively, root mean square error (RMSE) as 38.8 cc/m2/min and 0.126 g/m2/min, respectively, and coefficient of efficiency (CE) as 93.9% and 96.7%, respectively, during testing period.

Evaluation of Shear Bond Strengths of Three Adhesive Materials Used to Retain Fiber Post in the Severely Damaged Primary Anterior Teeth

Background and Objective: Tooth decay and periodontal problems are among the most common human diseases which are considered unsolved health problems in most countries of the world. This study aimed to investigate and compare the shear bond strength of the fiber post with three different types of cement, including Glass ionomer, as well as Universal and Flowable composites. Materials and Methods: This in-vitro study was conducted on 30 extracted anterior deciduous teeth with at least two-thirds of healthy roots. The teeth were randomly divided into three groups of 10. The teeth were mounted from the root part inside the self-curing acryl. Group 1 includes G-Premio Bond and Universal Composite, group 2 includes G-Premio Bond and flowable composite, and group 3 includes Glass ionomer without bond. Immediately, the fiber post was placed in the cemented material in the canal and was light-cured according to the manufacturer's instructions. Shear bond strength was performed by an electromechanical universal testing machine at a speed of 1 mm/min. Results: The shear bond strength mean values in the Universal composite, Glass ionomer, and Flowable composite groups were 15.29±4.82, 9.74±2.82, and 13.71±4.91 MPa, respectively, and the difference among them was significant (P=0.022). The shear bond strength mean value of the Universal composite was significantly higher than that of the Glass ionomer (P=0.020); however, this difference was not significant for comparing other groups (P<0.05). Conclusion: The highest and lowest amount of shear bond strength values for cementing fiber post in the repair of anterior deciduous teeth were related to the Universal composite and Glass ionomer cement groups, respectively, which showed a significant difference but did not differ significantly from the Flowable composite group

Tinospora sinensis (Lour.) Merr alkaloid rich extract induces colon cancer cell death via ROS mediated, mTOR dependent apoptosis pathway: “an in-vitro study”

BackgroundColorectal cancer (CRC) is the second most mortality rate causing disease after lung cancer. Though there is a significant improvement in the treatment schedule offered to CRC. However, there is no notable decrease in terms of cases as well as death rate. Hence, there is an urgent need to discover novel cancer therapeutics to treat CRC. Since ancient times, the use of phytochemicals has drawn huge attention as chemo-preventive and chemotherapeutic agents. Earlier studies on Tinospora sinensis (TS) revealed the cytotoxic effect on human colorectal carcinoma (HCT-116) cells, yet the mechanism is to be uncovered. Therefore, the present study was designed to study the cell death mechanism of TS in HCT-116 cells.MethodDifferent extracts such as n-hexane, ethyl acetate, and ethanol extracts from the root part of TS were prepared using a cold maceration process. The extracts were screened against cancer cell lines by methyl thiazoldiphenyltetrazolium bromide (MTT) assay. From the result, the most active extract was subjected to gas chromatography-mass spectrometry (GC-MS) and Fourier-Transform infrared spectroscopy (FTIR) analyses to identify the major constituents. Finally, the mechanism of cytotoxicity to cancer cells for the most active extract was evaluated using various experiments such as cell cycle analysis, Annexin-V assay, and Western blot.ResultsThe results from the MTT assay indicated that the n-hexane extract of TS inhibits the growth of HCT-116 cells more effectively than other cancer cells like Henrietta Lacks cervical cancer cells (Hela), and Michigan cancer foundation-breast cancer (MCF-7). The GC-MS and FT-IR analyses revealed the presence of alkaloids in the n-hexane extract and were responsible for the apoptosis activity in HCT-cells via reactive oxygen species (ROS) generation, and phosphoinositide 3-kinase (PI3K)/ protein Kinase B (Akt)/ mammalian target of rapamycin (mTOR) down-regulation.ConclusionThis study concludes that this finding is unique of its kind, and for the first time. The anticancer effect of TS root is specific to colon cancer cells (HCT-116). This distinctive finding helps the researchers to investigate further, and to identify a novel source for anti-colon cancer drug candidates in near future.

Impact of Irrigation with Contaminated Water on Heavy Metal Bioaccumulation in Water Chestnut (Trapa natans L.)

This study investigated the monitoring of six heavy metals (Cd, Cr, Cu, Fe, Mn, and Zn) in pond water, sludge, and cultivated water chestnut (Trapa natans L.) crops in Saharanpur district of Uttar Pradesh, India. For this purpose, samples of pond water, sludge, and T. natans plant (nut, shoot, and root) were collected in November of 2021 and 2022 from three zones (Zone 1: agricultural area irrigated with borewell water, Zone 2: urban area irrigated with municipal wastewater, and Zone 3: rural area irrigated with mixed type of wastewater) and subsequently analyzed for heavy metal concentration using atomic absorption spectroscopy. The results showed that the physicochemical and heavy metal characteristics of pond water and sludge were significantly (p < 0.05) higher in Zone 2 and 3 than to those in Zone 1. The concentration of heavy metals in T. natans root was comparatively high followed by shoot and nut parts. The bioaccumulation factor (BAF) was maximum while using pond water as a reference medium compared to pond sludge. Overall, the increasing order of heavy metals in T. natans was observed as Cd < Cr < Cu < Zn < Mn < Fe. In the edible part (nut) of T. natans, the average contents of Cd (0.005 ± 0.002 mg/kg), Cr (0.134 ± 0.009 mg/kg), Cu (1.043 ± 0.104 mg/kg), Fe (9.589 ± 0.957 mg/kg), Mn (4.326 ± 0.753 mg/kg), and Zn (1.540 ± 0.537 mg/kg) were comparatively less than shoot and root parts. Overall, the results revealed that T. natans irrigated with contaminated irrigation supplies at Zone 2 and 3 showed the highest BAF of heavy metals than Zone 1. Because the heavy metal concentrations in edible parts of T. natans did not exceed the threshold limits, the contaminated water sources in the Saharanpur region of India should be used in a safe and controlled manner.

Evaluation of Measurement Performance of Routine Chemistry Analytes by Application of Sigma Metrics, Method Evaluation Decision (MEDx) Charts and Quality Goal Index (QGI)

Objective: To evaluate performance of routine chemistry analytes in a tertiary care hospital laboratory by the application of sigma metrics. Introduction: Six sigma (6σ) is a popular Quality Management System (QMS) tool. Laboratories are increasingly using the six sigma method for the objective assessment and comparison of the analytical methods and instrument performance. Six sigma is about measuring or counting the number of defects. It quantifies the performance of a process as a rate of Defects-Per-Million-Opportunities (DPMO or DPM). The aim is to assess the performance and to eliminate or reduce the variation in a process. Materials and Methods: This prospective study was conducted over a period of six months duration. Sigma metrics were calculated using coefficient of variation (%CV), %Bias and total allowable error (%TEa). For %CV we used Internal Quality Control (IQC) samples, at two levels L1 and L2. Daily IQC results of L1 and L2 for 25 routine chemistry analytes were recorded in an excel sheet and %CV was calculated for each analyte for the period of study. For each analyte %Bias was calculated based on values obtained from monthly RIQAS-EQA program data. The total allowable error (%TEa) values for each analyte were extracted from various sources like Clinical Laboratories Improvement Amendment act (CLIA), Canadian Fixed Limits from the College of Physicians and Surgeons of Saskatchewan (CFX) and Spanish Society of Clinical Chemistry and Molecular Pathology (SEQC) table of Desirable Quality Specifications based on Biological Variation (BV) criteria for acceptable performance. Sigma values were calculated. The minimal acceptable performance criteria was considered as 3 sigma. Normalized MEDx charts were used to plot sigma metrics to visually present the performance. Quality Goal Index (QGI) analysis was carried out as a part of Root Cause Analysis (RCA). Results: Highest sigma value of 16.7 was noted for HDL-C and the lowest of 2.08 for chloride at level L1. Many analytes like ALP, Amylase, AST, CK, GGT, HDL-C, Magnesium and Uric Acid attained world class quality performance at both levels L1 and L2 with sigma levels of >6. Many other analytes showed satisfactory performance with sigma levels of >3. Sodium, potassium, chloride and urea did not show a satisfactory performance. Conclusion: Sigma metrics evaluation of analytical performance of our laboratory showed an acceptable performance for a wide range of analytes in patient samples.

Comparative assessment of the heavy metal phytoextraction potential of vegetables from agricultural soils: A field experiment

A field study was established to determine the phytoextraction potential of six vegetable species, namely Amaranthus viridis L., Basella alba L., Brassica chinensis var. Parachinensis, Brassica rapa L., Capsicum frutescens L., and Ocimum tenuiflorum L.. These edible plants were selected for their short growth cycles and high biomass production, which are some traits for efficient phytoremediation. Following acid digestion of the soil and vegetable samples using the USEPA 3050B acid digestion method, the extracts were analyzed for Al, Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, and Zn using ICP-OES. Results in soil samples showed that the concentrations of both beneficial and essential heavy metals, and non-essential heavy metals are below the WHO, USEPA, and CCME soil quality guidelines. Al is one of the highest concentrations found in the soil samples but it tends to accumulate in the root part of all vegetable species compared to the aboveground parts. In general, B. rapa L. accumulated the highest level of Cd (0.4 mg/kg) and Pb (5.71 mg/kg), while B. alba L. accumulated the highest Cr (2.62 mg/kg) in all plant parts. The findings in this study indicated that Co, Cu, Fe, Mn and Zn were mostly accumulated in leaves of A. viridis L. (Co, Cu, Fe, Mn and Zn), B. alba L. (Co, Fe and Mn), B. chinensis (Mn and Zn) and O. tenuiflorum L. (Mn), and roots of C. frutescens L. (Co, Cu, Fe and Mn), B. alba L. (Co, Cu and Zn), A. viridis L. and B. chinensis (Cu and Fe) and B. rapa L. (Fe). Cr, Pb and Ni were significantly greater in B. alba L. (Cr) And B. rapa L. (Ni and Pb) roots. MTF >1 was observed in the roots of all species for Co, Cd, Zn, and Ni. BTC values varied between the different vegetable species with A. viridis L. having the greatest heavy metal mobility between its plant parts and the best heavy metal phytoextraction potential among other species. The PCA biplots showed that heavy metals were partitioned differently between various plant parts of the vegetable species and can be explained by the first two components (PC1 and PC2) which were associated with the root and/or leaf parts for most vegetable species.

Biosynthesis of Silver Nanoparticles Using Salvia pratensis L. Aerial Part and Root Extracts: Bioactivity, Biocompatibility, and Catalytic Potential.

The aim of this research was the synthesis of silver nanoparticles (SPA- and SPR-AgNPs) using the aqueous extracts of the aerial (SPA) and the root (SPR) parts of the plant Salvia pratensis L., their characterization, reaction condition optimization, and evaluation of their biological and catalytic activity. UV-Vis spectroscopy, X-ray powder diffraction (XRPD), scanning electron microscopy with EDS analysis (SEM/EDS), and dynamic light scattering (DLS) analysis were utilized to characterize the nanoparticles, while Fourier transform infrared (FTIR) spectroscopy was used to detect some functional groups of compounds present in the plant extracts and nanoparticles. The phenolic and flavonoid contents, as well as the antioxidant activity of the extracts, were determined spectrophotometrically. The synthesized nanoparticles showed twice-higher activity in neutralizing 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) compared with the respective extracts. SPR-AgNPs exhibited strong antimicrobial activity against almost all of the tested bacteria (<0.0039 mg/mL) and fungal strains, especially against the genus Penicillium (<0.0391 mg/mL). Moreover, they were fully biocompatible on all the tested eukaryotic cells, while the hemolysis of erythrocytes was not observed at the highest tested concentration of 150 µg/mL. The catalytic activity of nanoparticles toward Congo Red and 4-nitrophenol was also demonstrated. The obtained results confirm the possibility of the safe application of the synthesized nanoparticles in medicine and as a catalyst in various processes.

Determination and Comparison of Bioactive Compounds in Different Parts of Glycyrrhiza Species

Glycyrrhiza spp., one of the most widely used herbal medicine for centuries in the world, contain a large number of bioactive such as triterpene saponins and flavonoids which are the main constituents and show broad biological activity. The present study aimed to evaluate the phytochemical profile of extracts from different parts (roots, stems and leaves) of all wild Glycyrrhiza spp. grown in Turkey and to reveal that other parts besides the roots are a rich source of bioactive compounds with potential use in the pharmaceutical and food industries. For this purpose, extracted bioactive compounds from different parts of five Glycyrrhiza spp. collected in different provinces of Turkey were determined and compared. The microwave-assisted extraction method, which is mentioned as a green technique and requires low solvent and extraction time, was applied. Determination of bioactives was performed using liquid chromatography- electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS). Among the collected Glycyrrhiza spp.; in leaf parts, the highest glycyrrhizin (GL) (2.05±0.07 mg g-1) and glycyrrhetinic acid (GA) (0.107±0.005 mg g-1) contents were found in Glycyrrhiza flavescens ssp. flavescens; the highest carbenoxolone (CBX) (0.133±0.006 mg g-1) and liquiritin (LQ) (1.644±0.014mg g-1) contents were in Glycyrrhiza glabra grown in Polatlı. In stem parts, the highest GL (2.735±0.04 mg g-1), CBX (0.069±0.004 mg g-1) and LQ (0.602±0.010 mg g-1) contents were determined in G. glabra plant growing in Ankara. In root parts, the highest GL (14.68±0.09 mg g-1) and LQ (9.735±0.046 mg g-1) contents were detected in G. glabra plant growing in Gaziantep while the highest GA (0.136±0.005 mg g-1) and CBX (0.188±0.067 mg g-1) contents in Glycyrrhiza flavescens ssp. antalyensis. Thus, it was determined which location in Turkey and which parts of Glycyrrhiza spp. that grow wild in Turkey can be used as a priority for the food and pharmaceutical industry with this study.

Impact of Rhamnolipids (RLs), Natural Defense Elicitors, on Shoot and Root Proteomes of Brassica napus by a Tandem Mass Tags (TMTs) Labeling Approach.

The rapeseed crop is susceptible to many pathogens such as parasitic plants or fungi attacking aerial or root parts. Conventional plant protection products, used intensively in agriculture, have a negative impact on the environment as well as on human health. There is therefore a growing demand for the development of more planet-friendly alternative protection methods such as biocontrol compounds. Natural rhamnolipids (RLs) can be used as elicitors of plant defense mechanisms. These glycolipids, from bacteria secretome, are biodegradable, non-toxic and are known for their stimulating and protective effects, in particular on rapeseed against filamentous fungi. Characterizing the organ responsiveness to defense-stimulating compounds such as RLs is missing. This analysis is crucial in the frame of optimizing the effectiveness of RLs against various diseases. A Tandem Mass Tags (TMT) labeling of the proteins extracted from the shoots and roots of rapeseed has been performed and showed a differential pattern of protein abundance between them. Quantitative proteomic analysis highlighted the differential accumulation of parietal and cytoplasmic defense or stress proteins in response to RL treatments with a clear effect of the type of application (foliar spraying or root absorption). These results must be considered for further use of RLs to fight specific rapeseed pathogens.

Insight into the phytochemical, biological, and in silico studies of Erythrina suberosa roxb.: A source of novel therapeutic bioactive products from a medicinal plant

BackgroundErythrina suberosa (E. suberosa) Roxb. also known as corky coral tree is used as a folklore medicine to treat ulcers and piles. This study explored the phytochemical profiles of methanol and dichloromethane (DCM) extracts from the aerial, root, and flower parts of E. suberosa. MethodTotal bioactive contents, high-performance liquid chromatography array (HPLC-PDA) polyphenolic quantification, and ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) analysis were used to evaluate the phytochemical profile. Biological potential was evaluated by determining their antioxidant (DPPH, ABTS, FRAP, CUPRAC, Phosphomolybdenum, and metal chelating) and enzyme inhibitory (cholinesterase, amylase, glucosidase, and tyrosinase) effects. ResultsThe phenolic (46.26 mg GAE/g extract) and flavonoid (32.46 mg QE/g extract) contents were found to be highest in the methanolic extract of roots (EsR-M). HPLC-PDA analysis determined the presence of numerous bioactive compounds, including catechin, syringic acid, p-hydroxy benzoic acid, gallic acid, vanillic acid, epicatechin, rutin, chlorogenic acid, carvacrol, sinapinic acid, and naringenin in considerable amounts. A total of 143 phytochemicals, belonging to phenolic, flavonoid, coumarin, terpenoid, chalcone, saponin, and glycoside classes, were tentatively identified by UHPLC-MS analysis. Aerial methanolic extract (EsA-M) exhibited highest radical scavenging (DPPH 86.81 mgTE/g extract, ABTS 245.17 mgTE/g extract) as well as reducing potential (FRAP 177.12 mgTE/g extract, CUPRAC 177.12 mgTE/g extract). Likewise, it was also noted to be most active for BChE (5.70 mg GALAE/g extract) and tyrosinase inhibition (148.35 mg KAE/g extract). While, roots-DCM extract (EsR-D) presented considerable inhibition for AChE (4.69 mg GALAE/g extract) and amylase (0.81 mmol ACAE/g extract) enzymes. Coreopsin, davallioside A, and isovitexin were docked to tyrosinase to depict possible interaction. ConclusionThis study has established baseline data on the biological and phytochemical profile of E.suberosa which was found to possess antioxidant and enzyme inhibition properties which tend to validate its traditional uses. However, further studies to identify the molecular targets responsible for these activities are warranted which could be explored as a functional food.

Method for solving inverse spectral problems on quantum star graphs

Abstract A new method for solving inverse spectral problems on quantum star graphs is proposed. The method is based on Neumann series of Bessel function representations for solutions of Sturm–Liouville equations. The representations admit estimates for the series remainders which are independent of the real part of the square root of the spectral parameter. This feature makes them especially useful for solving direct and inverse spectral problems requiring calculation of solutions on large intervals in the spectral parameter. Moreover, the first coefficient of the representation is sufficient for the recovery of the potential. The method for solving the inverse spectral problem on the graph consists in reducing the problem to a two-spectra inverse Sturm–Liouville problem on each edge. Then a system of linear algebraic equations is derived for computing the first coefficient of the series representation for the solution on each edge and hence for recovering the potential. The proposed method leads to an efficient numerical algorithm that is illustrated by a number of numerical tests.

In situ analysis of volatile oil in Angelica sinensis roots by fluorescence imaging combined with mass spectrometry imaging

In this study, the spatial distribution and accumulation dynamics of volatile oil in Angelica sinensis roots was realized by fluorescence imaging combined with mass spectrometry imaging. The laser scanning confocal microscopy was used to determine the optimal excitation wavelength and the fluorescent stability of volatile oil in the sections of Angelica sinensis roots. The results demonstrated that 488 nm was the most suitable excitation wavelength for the identification and quantitative analysis of volatile oil. It was observed that volatile oil accumulated in the oil chamber of the phelloderm and secondary phloem, and the oil canal of the secondary xylem. The results also indicated that there were differences in content during different periods. Furthermore, the MALDI-TOF-MSI technology was used to study the spatial distribution and compare the chemical compositions of different parts of Angelica sinensis roots during the harvest period. A total of 55, 49, 50 and 30 compounds were identified from the head, body, tail of the root and root bark, respectively. The spatial distribution of phthalides, organic acids and other compounds were revealed in Angelica sinensis roots. The method developed in this study could be used for the in situ analysis of volatile oil in Angelica sinensis roots.

Root Yield and Starch Synthase Type IV Gene Activity under Different Micro Nutrient Fertilizer and Harvest Ages on Cassava (Manihot esculenta Crantz)

Abstract The important part of cassava root is starch which is probably controlled by starch synthase type IV (SSIV) gene. The information of micro nutrient and harvest age related to the activity starch synthase type IV (SSIV) gene is still very rare. The objectives of this study were to evaluate root fresh weight of cassava, to compare yield of storage root, and to evaluate the activity of starch synthase type IV (SSIV) gene by real-time quantitative polymerase chain reaction (RT-PCR) applied by micro nutrient fertilizer. Treatments were arranged by factorial (3 × 3) in randomized complete block design (RCBD) with three replications used as block. The first factor was three different dosages of micro nutrient fertilizer as 0, 20, and 40 kg/ha. The second factor was harvest ages as 7, 8, and 10 months after planting (MAP). The micro nutrient fertilizer mainly contents of 5,888 ppm Fe and 1,368 ppm Zn. Variables were leaf number (LN), leaf fresh weight (LFW), leaf dry weight (LDW), stem fresh weight (SFW), stem dry weight (SDW), root fresh weight (RFW), root dry weight (RDW), skin root fresh weight (SRFW), skin root dry weight (SRDW), starch content, and activity of SSIV gene. The result showed that RDW of cassava applied by 40 kg micro nutrient/ha was significantly increased at 10 MAP. The increase in RDW was due to mainly high SSIV gene activity. Additionally, the SSIV gene activity caused by 20 kg micro nutrient/ha treatment showed almost as twice as those by 40 kg micro nutrient/ha. Keywords: Real-time PCR, Root dry weight, Root yield, Starch, Stem dry weight

Effect of different sowing densities and substrates on the growth of arugula seedlings

Arugula is known for the spicy flavour of its leaves, and it is an important crop due to its high productivity and multiple harvests per year. However, seedling quality may limit its production. This study examined arugula seedling emergence and production at different sowing densities and substrate compositions. In a greenhouse, the broadleaf cultivar was randomized with a 5×3 factorial design (substrate compositions: 100% medium textured sand (SD), 100% Carolina Soil® commercial substrates (CS), 50%SD+50%CS, 75%SD+25%CS, and 25%SD+75%CS, and sowing densities: 5; 10 and 15 seeds/alveolus); with five replications. Analyzes were carried out on emergence speed index and mean emergence time, as well as on relative frequency, lengths of aerial and root parts, number of leaves, and rating scales according quality for tuft formation (or bouquets) and clod stability. All seeding densities and substrate compositions showed good seedling formation, except for the 100% SD composition, which showed low adherence in the substrate-plant-container-water system for tuft formation and clod stability. Except for 100% SD composition, an average of 70% of seedling emergence was at 9.7 d. For the production of commercial seedlings, it is indicated the use of 10 or more seeds per alveolus, and all substrate compositions tested in this study, except 100% SD.

Determination of the optimal arrangement of engines along the wingspan

A modern gas turbine engine, in the layout of the designed aircraft, allows us to consider fundamentally different placement options. The contradictory influence of the type, number and location of engines on the safety and efficiency of flight leads to the need to study these tasks at the early stages of design. This article discusses an approach to determining the optimal position of engines by wingspan at the stage of preliminary design. The calculation features consist in taking into account various factors: strength, for different design cases, mass-inertial, operational-technological and others. In the course of the study, the aircraft wing power set was simulated with its subsequent calculation in the NX system. As a result of the study, the optimal arrangement of engines for a four-engine aircraft with known geometric characteristics of the wing and its shape was obtained. An approach is shown, as a result of which it is possible to determine the optimal position of the engines, both in terms of the magnitude of bending moments in the root part of the wing and moments of inertia. The same method can be used to prove or correct the already accepted position of the engines on the designed aircraft.

Инновационные методы лечения пульпитов постоянных зубов с несформированными корнями у детей

When treating pulpitis of permanent teeth with unformed roots in children, the dentist primarily relies on the knowledge of the morphological structure of their root systems. Therefore, currently, when choosing the tactics of endodontic management of such children preference is given to classical techniques, in particular - vital amputation. The latter involves necrectomy of the infected crown part of the pulp, while the root part remains intact and is covered with a therapeutic agent. This condition is necessary for the successful completion of apexification, i.e. the closure of the root apex during its development stage. A wide range of drugs that stimulate apexification is available when performing a vital amputation. The most effective of these are preparations based on calcium hydroxide, mineral trioxide aggregate (MTA) and paraformaldehyde. However, clinical experience shows that these preparations are not without significant drawbacks, the main of which is dehydration of root dentin, which contributes to the fragility of the root and therefore increases the risk of root fracture of the tooth. Furthermore, treatment with calcium-based formulations is lengthy and requires a large number of visits. To address these shortcomings, the American Association of Endodontics has proposed an alternative technique, revascularization (regenerative endodontic treatment), based on irritation of periapical tissues by intensive instrumentation of root canals to stimulate bleeding and form a blood clot containing induced stem cells and growth factors that promote apex closure. In this regard, this literature review describes clinical studies of recent years containing a comparative analysis of the most common drugs used in the treatment of pulpitis in teeth with unformed roots by the method of vital amputation, as well as the latest prospective studies containing the results of clinical application of the revascularization technique.

Two and a Half Years Follow of the Pontic Site Development with Root Submergence Technique: A Case Report

A soft tissue drape that is formed around the tooth, as it emerges from the bone, has both aesthetic and functional significance. Post-extraction soft and hard tissue changes are inevitable and it leads to a buccal concavity. The pontics for tooth or implant supported fixed partial denture in such cases appear longer. Preservation of a part of root preserves the periodontal apparatus and hence facilitates the optimal region for the pontic site development. This case report describes root submergence technique for pontic site development in maxillary anterior tooth-supported FPD with a follow up of two and a half years.

Evaluation of Anticholinergic, Antidiabetic and Antioxidant Activity of Astragalus dumanii, an Endemic Plant

The research was conducted to separately evaluate and detect the possible in vitro antioxidant, antimicrobial activity of ethanol extracts prepared from aerial parts and roots of Astragalus dumanii and anti-cholinesterase and α-glucosidase inhibitory activity from only aerial parts of its The antioxidant capacity was tested by scavenging of DPPH and ABTS free radicals. Compared with the standard antioxidant compound gallic acid; Root and aerial part extract showed lower DPPH radical scavenging activity, however aerial part extract demonstrated higher ABTS radical scavenging activity. The phenolic contents were detected as 5.31 ± 0.03 and 13.23 ± 0.05 mg gallic acid equivalent g-1 extract, flavonoid contents were found as 8.26 ± 0.004 and 7.93 ± 0.005 mg Qercetin equivalent g-1 extract. In addition, the effects of the extracts obtained from aerial parts of the plant on acetylcholinesterase, butyrylcholinesterase and a-glycosidase enzymes were investigated in vitro and IC50 values were obtained as 1.47, 0.83 and 0.48 µg mL-1, respectively. When these values were compared with standard substances, it was seen that Astragalus dumanii could be a good enzyme inhibitory agent. Antimicrobial activity of the plant extracts were determined using the microdilution method and the extracts was not observed to have any antimicrobial activities..