Developing low-dimensional spintronic materials with room temperature magnetic ordering and large spin polarization is the key for the fabrication of practical spintronic devices with a high circuit integration density and speed. Here, first-principles calculations were performed to systematically investigate a two-dimensional hexagonal MnN monolayer with room temperature magnetic ordering and 100% spin polarization. The MnN monolayer is thermally, dynamically, and mechanically stable, and intrinsically half-metallic with a very wide band gap. The Curie temperature of the MnN monolayer is estimated to be ∼368 K, which is higher than room temperature and insensitive to strain. The MnN monolayer shows half-metallic and excellent magnetic stability upon the external strain from −10 to 10%. Our calculations indicate that the MnN monolayer could be a promising material for room temperature spintronic devices.