The main objective of this study was to make out the decontamination effects of bacillus atrophaeus in different locations by Vaporous Hydrogen Peroxide decontamination equipment which was designed independently. Bacillus atrophaeus were in different locations of the room, the room volume is 67m3. Then the Vaporous Hydrogen Peroxide (VHP) decontamination equipment was opened to sterilize the room. After the sterilization, based on the Technical Standard For disinfection, the biological indicators including bacillus atrophaeus were incubated at37 °C for 48 hr,resultant colonies were enumerated. The BI results showed that along with the increase of distance between VHP decontamination equipment and the BI, decontamination time gradually extended. When the distance were 2m, 4m and 6m, the decontamination time were 15min, 30min and 30min. When the BI was put in the room corner, the decontamination time was 60min. From this paper,it can be determined that the VHP technology is a valid approach to the sterilization of interior space.