Dietary Rice Bran Research Articles

Use of defatted fermented rice bran in the diet of juvenile mullets Mugil liza

The study aimed to evaluate the use of increasing levels of defatted rice bran fermented by Rhizopus oryzae in the diets of juveniles of mullet fish Mugil liza, assessing growth parameters, proximal carcass composition, and lipid peroxidation (TBARS) in the liver and intestine. Six experimental diets were formulated: a control treatment without rice bran, a second treatment with the inclusion of 200 g kg−1 of defatted rice bran without fermentation (DRB200), and the remaining treatments received diets with the inclusion of 100, 200, 300, or 400 g kg−1 of fermented bran. The experimental design used 216 mullet juveniles (2.10 ± 0.04 g) randomly distributed in a recirculation system composed of 18 tanks, divided into six treatments and three replications, with 12 fish per experimental unit. During the experiment, fish were fed ad libitum three times a day, for 50 days. No mortalities were recorded during the experimentation. The performance variables showed that an inclusion equal or higher than 200 g kg−1 of fermented rice bran resulted in decreased growth. Furthermore, in carcass proximal composition, the lipid content decreased with higher inclusion levels of fermented rice bran and the control treatment showed the highest lipid content, while the ash content of fish carcasses increased with the higher inclusion of the tested ingredient. No differences were recorded for TBARS in the liver and intestine. According to the results, we recommend a maximum inclusion of 100 g kg−1 fermented rice bran in diets for M. liza juveniles.

Comparative digestibility of energy, dry matter, and nutrients by gestating and lactating sows fed corn–soybean meal diets without or with full-fat or defatted rice bran

Twenty-four gestating sows and 24 lactating sows were randomly allotted to three diets with eight replicate sows per treatment in a 3 × 2 factorial arrangement. Different sows were used in gestation and lactation periods. The hypothesis was that digestibility of gross energy (GE), dry matter (DM), and nutrients in lactating sows is not different from that in gestating sows. A corn–soybean-meal diet and two full-fat-rice-bran or defatted rice bran diets were used. Results indicated that regardless of diet, lactating sows had greater (P < 0.01) apparent total tract digestibility of GE, DM, neutral detergent fiber, organic matter, and phosphorus than gestating sows.

Abstract 2343: Fecal metabolic changes following rice bran supplementation in the murine model for colitis associated cancer of the colon

Abstract Dietary rice bran mediated inhibition of colon carcinogenesis has been described via multiple mechanisms including, but not limited to, reduced cell proliferation, induction of apoptosis and modulation of arachidonic acid metabolism. Human clinical research further supports rice bran modulation of gut microbiome metabolism for enhancing colonic health. Little is known regarding the time series of fecal metabolite changes involved in the progression of colon cancer in Azoxymethane (AOM)/Dextran Sodium Sulfate (DSS) treated mice and modulation of metabolic pathways by dietary rice bran supplementation. This study investigated the metabolic changes associated with rice bran supplementation during colitis-associated cancer over 14 weeks. Conventional adult male BALB/c mice were fed control diet (AIN93) or 10% w/w rice bran diet during AOM-DSS treatment. Global, non-targeted metabolomics was applied to murine fecal samples following consumption of rice bran after 2 days, 2, 6, 10 and 14 weeks and compared to mice fed control diet. Raw data was extracted, peak-identified and processed using Metabolon's hardware and software. A total of 702 biochemicals, 592 compounds of known identity and 110 compounds of unknown structural identity were reported, and ANOVA contrasts used to identify biochemicals that differed significantly between experimental groups. Random forest analysis based on an ensemble of decision trees revealed dietary distinctions in 30 metabolites at 10wk and 14wk with a predictive accuracy of 92% and 75%, respectively. These included vitamins, thiamine (vitamin B1), retinol (vitamin A) pyridoxine (vitamin B6), pyridoxal and pyridoxate) and cofactors, quinolinate, 1-methylnicotinamide and trigonelline, with therapeutic, antioxidant and anti-inflammatory properties. There was also fold-increases in rice bran-derived phenolics, namely 2-hydroxyhippuarte, 3-(3-hydroxyphenyl)propionate and 3-phenylpropionate at both 10 and 14 weeks when compared to control. The bioavailability of these bioactive phenolic compounds was further supported by colonic tissue metabolite profiling. Shifts in cellular energy metabolism, shown by citrate, aconitate, and malate; and in lipid metabolites namely (glycerol 3-phosphate, monoacylglycerols (1-oleoylglycerol (18:1) and 2-linoleoylglycerol (18:1)) and diacylglycerols (palmitoyl-linoleoyl-glycerol (16:0/18:2) and oleoyl-linoleoyl-glycerol (18:1/18:2) were detected as novel mechanisms for rice bran mediated-inhibition of colon cancer. TCA cycle metabolites may reflect the favorably altered intestinal microenvironment for depletion of cancer cells. This study supports a time series of fecal metabolic profiles associated with dietary rice bran alongside identification of novel mechanism(s) by which rice bran protects against or prevents progression of colon cancer. Citation Format: Petronella R. Hove, Annika Weber, Bridget Baxter, Hend Ibrahim, Kristopher Parker, Komal Raina, Elizabeth P. Ryan. Fecal metabolic changes following rice bran supplementation in the murine model for colitis associated cancer of the colon [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2343.

Effect of Rice Bran on Productivity, Hatchability and Yolk and Blood Lipid Profile of Laying Japanese Quail

A total number of 216 female and 108 male Japanese quail at 14 wks of age were used in a 7-week-trial to investigate the effect of rice bran on the quality and quantity of egg production as well as blood lipid profile. There were three dietary treatment groups: a control group fed on a corn-soybean based diet, 12.5 and 25.0 % rice bran diets. Results showed that egg laying rate, egg weight, egg mass and feed conversion ratio were insignificantly affected by treatments. Egg quality insignificantly affected by different treatments. The results showed significant decrease in egg yolk total lipids and cholesterol due to inclusion of rice bran in the diets in comparison with the control group. Hatchability and fertility was not influenced by different treatments. Blood serum total lipids, triglycerides, low density lipoprotein were significantly decreased, however, high density lipoprotein increased with increasing level of rice bran in the diet. Rice bran treatments showed significant (P ≤ 0.01) increase in concentration of TAC than control group reached to 32.6 and 66.3 % for birds fed 12.5 and 25 % rice bran containing diets, respectively. Numerical increase in glutathione peroxidase in the group given 12.5 and 25 % rice bran containing diet as compared to the control group. In conclusion, egg laying rate and egg quality insignificantly affected by rice bran, however, the results showed significant improvements in blood and egg lipid profile, beside the antioxidative status as a result of inclusion fresh rice bran in the diet.

Evaluation of the population growth and fatty acid composition of Copepoda, Oithona nana, fed on different diets

The marine Copepoda species Oithona nana , is considered as one of the most Copepoda species that successfully mass cultured in marine hatcheries. This study investigated the effects of four feed diets (soybean, yeast, rice bran, and corn starch) on the population growth, growth rate, population composition, fecundity, and fatty acid composition of Copepoda, O. nana . The experiment was continued for 15 days and the copepods were fed on four feed diets with concentration of 1 g/10 6 individual/day. The results found that O. nana fed on corn starch showed the highest significant population growth (9067 Individual/L) and population growth rate (0.735). For nutritional value, copepods fed on rice bran were detected to have the highest content of monounsaturated fatty acid (MUFA), polyunsaturated fatty acids (PUFA), the lowest saturated fatty acids/unsaturated fatty acids ratio (SFA/UFA ratio) and the lowest SFA. More importantly, the rice bran diet was the only diet that showed eicosapentaenoic acid (EPA; C20:5ω3). Moreover, copepods fed on rice bran showed the highest significant female fecundity (8.33 egg/female), copepodite and nauplii percentages (33.27 and 32.65%, respectively). Finally, regarding to the quantity, corn starch is the most suitable diet for mass culturing O. nana, while, regarding to the quality, rice bran enhances the nutritional value and fecundity of the Calanoida Copepoda O. nana.

Population Dynamics, Fecundity and Fatty Acid Composition of Oithona nana (Cyclopoida, Copepoda), Fed on Different Diets.

Simple SummaryMarine larval production is the most critical stage in the life of the marine aquacultured species, which depends on the use of different zooplanktonic organisms as live feed. Copepods are high-quality live prey that could be efficiently used to overcome the transition period from live food to weaning with an artificial diet in the post-larval stages. The main culture systems of copepods use microalgae as uni-food, nevertheless for the more sustainable and cost-efficient production of copepods, the development of artificial diets is the core of its production techniques. The present study was conducted to improve the production and nutritional quality of copepod, Oithona nana, using different diets (soybean, yeast, rice bran, and corn starch). Among all diets, corn starch revealed the highest population growth. Meanwhile, animals nourished by rice bran showed the highest percent of copepodite, nauplii, and fecundity. The nutritional quality of copepods referred to fatty acids profile showed a high percentage of unsaturated fatty acids in copepods fed on rice bran. In conclusion, the dry feeds are very applicable, more economic, and simply alternative diets to substitute microalgae and maximize the fecundity and population of O. nana in fish hatcheries.The marine copepod species Oithona nana is considered as one of the most successfully mass cultured Cyclopoida species in marine hatcheries. This study investigated the effects of four feed diets (soybean, yeast, rice bran, and corn starch) on the population growth, growth rate, population composition, fecundity, and fatty acid composition of native isolated Cyclopoida copepod species O. nana. The experiment was continued for 15 days and the copepods were fed on one of the four diets with a concentration of 1 g 10−6 individual day−1. The results revealed that corn starch was found to be the most supportive diet for population growth and population growth rate. For nutritional value, copepods fed on rice bran were detected to have the highest content of MUFA, PUFA, and the lowest SFA and SFA/UFA ratio; more importantly, the rice bran diet was the only treatment that showed C20:5ω3. Moreover, copepods fed on rice bran showed the highest significant female fecundity, copepodite, and nauplii percent. Finally, the protocols described in the current study concluded that the dry feeds, especially corn starch, are very useful and applicable in hatcheries for maximizing the fecundity and density of Cyclopoida copepod species, O. nana.

Effect of rhizopus stolonifer fermented cocoa pod husk meal supplemented with enzyme on growth performance, heamato-biochemical indices and sexual maturity of pullet chicken

Effect of rhizopus stolonifer fermented cocoa pod husk meal supplemented with enzyme on growth performance, heamato-biochemical indices and sexual maturity of pullet chicken

Dietary Rice Bran-Modified Human Gut Microbial Consortia Confers Protection against Colon Carcinogenesis Following Fecal Transfaunation.

Rice bran, removed from whole grain rice for white rice milling, has demonstrated efficacy for the control and suppression of colitis and colon cancer in multiple animal models. Dietary rice bran intake was shown to modify human stool metabolites as a result of modifications to metabolism by gut microbiota. In this study, human stool microbiota from colorectal cancer (CRC) survivors that consumed rice bran daily was examined by fecal microbiota transplantation (FMT) for protection from azoxymethane and dextran sodium sulfate (AOM/DSS) induced colon carcinogenesis in germ-free mice. Mice transfaunated with rice bran-modified microbiota communities (RMC) harbored fewer neoplastic lesions in the colon and displayed distinct enrichment of Flavonifractor and Oscillibacter associated with colon health, and the depletion of Parabacteroides distasonis correlated with increased tumor burden. Two anti-cancer metabolites, myristoylcarnitine and palmitoylcarnitine were increased in the colon of RMC transplanted mice. Trimethylamine-N-oxide (TMAO) and tartarate that are implicated in CRC development were reduced in murine colon tissue after FMT with rice bran-modified human microbiota. Findings from this study show that rice bran modified gut microbiota from humans confers protection from colon carcinogenesis in mice and suggests integrated dietary-FMT intervention strategies should be tested for colorectal cancer control, treatment, and prevention.

Comparative value of kola testa, corn-bran and rice- bran with maize for mature african giant land snail (Archachatina marginata)

This study was designed to compare the effect of kola testa, which is rich in fibre with maize the conventional energy source and other sources of fibre such as corn bran and rice bran. A total of two hundred and forty African giant land snails (Archachatina marginata) with an average live weight of 177.69 ± 10.87g were assigned to four dietary treatments: a maize-based diet (Diet 1) served as control, in a completely randomized design. The other diets were kola testa - based (Diet 2), corn bran-based (Diet 3) and rice bran-based (Diet 4) diets. The percentage of each test ingredient and that of maize was 31.30g/100g Each treatment consisted of sixty growing snails, which were subdivided into 3 replicates of 20 snails each. The snails received the diets over a period of 20 weeks. Weight gain was significantly higher (P<0.05) for the kola testa-based diet in comparison with the corn and rice bran-based diets. Depression of magnesium and iron in the shell, flesh and fluid of snails fed corn and rice bran diets was observed. The trial revealed that kola testa-based diet compared favorably well with the corn and rice bran-based diets.

The effect of fibre sources on the performance of young rabbits

A feeding trial lasting 84 days was conducted using 45 weaned rabbits (with weught range 643-652g) to investigate the effect of using millet husk (MH), Rice bran (RB) and Sorghum panicle (SP) as fibre sources for growing rabbits in a completely randomized design. Forty-five young rabbits were randomly assigned to 3 different groups. Fifteen rabbits per treatment. MH, RB, and SP were randomly incorporated at the rate of 150g/kg of diets. The diets were isonitrogenous (16% CP). The effect of these fibre sources on average daily feed intake (ADFI), average daily gain (ADG), feed efficiency nutrient digestion was determined. Results of the study showed that rabbits on MH based diets had a significantly higher (P>0.05) ADFI, feed efficacy and final weight. Though the results of the ADG and average daily crude fibre intake was highest in the MH diet, it was not significantly (P>0.05) different from the results obtained for RB. The RB diet had a better digestibility of all nutrients. This was however not reflected in the performance of the rabbit. It was concluded that different sources of fibre affect the performance of rabbits differently. In this study, MH was superior to MB and SP as a fibre source for growing rabbits. It is therefore necessary to study the effect of the different fibre sources before incorporating them in concentrate diets.

Response of starter broilers to diets containing graded levels of rice bran with or without palm oil

One hundred and forty four(144) day old broiler chicks of hubbard breed from a commercial hatchery were used to investigate the response of broiler chick to 0, 10 and 20% dietary rice bran with or without palm oil in a 5 week feeding trial. Significant interaction between dietary levels of rice bran and palm oil were observed on feed intake, weight gain and feedgain ratio (P>0.05). Increase in dietary levels of rice bran deduced feed transit time in the GIT (P>0.05) while supplementary palm oil had the opposite effect (P>0.05). There was a tendency for dietary supplementation of palm oil to increase protein or fat retention (P>0.05) but had no significant effect on crude fibre retention (P>0.05) but had no significant effect of crude fibre retention (P>0.05). It was concluded that broiler chicks could tolerate up to 10% dietary levels of rice bran without detrimental effects on performance and nutrients retention. With supplementary 5% palm oil added to such a diet, performance was comparable to that of birds fed a standard diet with money saved in the process

181 Effects of Aspergillus oryzae prebiotic on energy and nutrient digestibility of growing pigs

Abstract The objective of this study was to determine the effects of an Aspergillus oryzae prebiotic (AOP, Amaferm®) on nutrient digestibility in growing pigs fed high fiber diets. Eighteen growing barrows (initial BW = 50.60 ± 4.90 kg) were surgically equipped with a T-cannula at the distal ileum. Three diets were formulated by including 29.65% corn-distillers dried grains with solubles (DDGS), 36.65% rice bran (RB) or 24.59% wheat middlings (WM) in corn and soybean meal-based diets to meet nutrient requirements for 50 to 75 kg growing pigs. Three additional diets were formulated by supplementing 0.05% AOP at the expense of corn in DDGS (DDGS + AOP), RB (RB + AOP), and wheat middlings (WM + AOP) diets. Pigs were allotted randomly to a triplicated 6 × 2 Youden square design with 6 diets and 2 successive periods. Feces and ileal digesta were collected for 2 d after a 21 d adaptation period, and nutrient content was analyzed to calculate apparent total tract digestibility (ATTD) and apparent ileal digestibility (AID). Standardized ileal digestibility (SID) of amino acids was calculated by correcting AID with basal endogenous amino acid losses determined from the same set of pigs. Supplementation of 0.05% AOP increased (P < 0.05) ATTD of DM, GE, CP, NDF, and ash in DDGS, RB, and WM diets. Diet DE was 35 kcal/kg greater (P < 0.05) in pigs fed AOP supplemented diets compared with those fed diets without AOP. Pigs fed DDGS+AOP diet had greater (P < 0.05) AID of ether extract compared to those fed DDGS diet. However, supplementation of AOP did not (P > 0.05) affect AID of GE, DM, CP, NDF, ash or SID of amino acids. In conclusion, supplementation of AOP in high fiber diets containing DDGS, RB, or WM increased total tract energy value and nutrient digestibility.

Feeding Arsenic-Containing Rice Bran to Growing Pigs: Growth Performance, Arsenic Tissue Distribution, and Arsenic Excretion.

This research was conducted to study the growth performance, arsenic (As) tissue distribution, and As excretion of pigs fed As-containing rice bran. Twenty gilts (26.3 kg) were randomly assigned to 3 dietary treatments (n = 6 or 7) with Diets I, II, and III containing 0, 36.7, and 73.5% rice bran and 0, 306, and 612 ppb As, respectively. Pigs were fed for 6 weeks, and their growth performance and daily activities were examined. Fecal, blood, and hair samples were collected immediately before and after the 6-weeks. At the end of the 6-weeks, pigs were slaughtered; the liver, kidney, muscle, and urine samples were collected. No pig showed any unhealthy signs throughout the trial. The average daily feed intake, average daily gain, and final body weight of Diet III pigs were lower (p ≤ 0.001) than Diet I pigs. The gain to feed ratios were not different among the treatments. The fecal, hair, kidney, and urinary As concentrations of both Diets II and III pigs were higher than Diet I pigs. The hair As concentration of Diet III pigs was higher than Diet II pigs, but no difference was found in the fecal, urinary, kidney, or muscle As concentrations between Diets II and III pigs. The blood and muscle As concentrations were below 10 ppb. These results suggest that 73.5% dietary rice bran inclusion compromised growth performance, whereas the 36.7% inclusion did not. The fecal As data imply that dietary As was poorly absorbed by the gastrointestinal tract. The tissue As data indicate that the absorbed As was rapidly cleared from the blood with some retained in various organs and others eliminated via urine. The hair As concentration was much higher than that of liver and kidney. The muscle As data suggest that the pork produced from the pigs fed a typical As-containing rice bran as used in this study is safe for human consumption.

Relationship between Dietary Fiber Fermentation and Volatile Fatty Acids' Concentration in Growing Pigs.

Simple SummaryThe study suggests differences in fermentable capacity of fibrous feed ingredients are associated with fiber composition in pig. Results demonstrate that the fiber digestibility of oat bran, sugar beet pulp and soybean hulls is greater than for corn bran, wheat bran and rice bran in the pig intestine. Furthermore, results indicate that volatile fatty acids’ concentration (VFA) is positively correlated primarily with insoluble dietary fiber (IDF) fermentation, and the digestibility of IDF is the best single variable to predict fecal VFA concentrations. The contribution of this study is to provide instructions on how to implement fiber-rich ingredients effectively in the feed formulation for swine.This study was conducted to determine whether differences in fiber fermentation in fiber-rich feed ingredients exist and to assess relationship between fiber fermentation and concentration of volatile fatty acids (VFA) in pig. Castrated males (barrows) were allotted randomly to six diets formulated with different amounts of wheat bran (WB), corn bran (CB), sugar beet pulp (SBP), oat bran (OB), soybean hulls (SH) or rice bran (RB). The apparent ileal digestibility (AID) of soluble dietary fiber (SDF) for OB and SH diets was greater (P < 0.05) than for the other diets. The fermentation of total dietary fiber (TDF) and insoluble dietary fiber (IDF) in the hindgut were greater (P < 0.05) for SBP and SH diets than for WB, CB, OB and RB diets. The apparent total tract digestibility (ATTD) values of all fiber components in SBP, SH and OB diets were greater (P < 0.05) than for WB, CB and RB diets. The concentration of VFA in feces was positively correlated with the ATTD of IDF and cellulose, and ATTD of IDF is the best factor for predicting fecal VFA concentration. Overall, dietary fiber source affected fermentable characteristics of fiber components in the different digestive segments of pig intestine.

Effect of Dietary Supplement, Base Mix, and/or Soybean Meal on Growing Pig Performance

A total of 60 crossbred pigs (Yorkshire × Duroc, initially 56.5 kg) were used in a 42-day trial to evaluate the effect of replacing a commercial high protein supplement with soybean meal and/or a base mix in rice bran-based diets on pig growth performance. Pigs were allotted to pens by body weight and pens were randomly assigned to dietary treatments in completely randomized design. There were 4 dietary treatments with 3 or 4 replicate pens per treatment and 4 pigs per pen. Dietary treatments were: 1) 80% rice bran with 20% high protein supplement (RBS20), 2) 95% rice bran with 5% high protein supplement (RBS5), 3) 97.5% rice bran with 2.5% base mix (RBB2.5), and 4) 92.5% rice bran with 5% soybean meal and 2.5% base mix (RBSBB). The base mix included vitamins, macro and trace minerals, L-Lysine, L-Threonine, and DL-Methionine. Overall (d 0 to 42) average daily gain was greater (P < 0.001) for pigs fed RBSBB and RBS20 diets than pigs fed the RBS5 or RBB2.5 diets. Pigs fed the RBS5 diet had reduced (P < 0.004) average daily feed intake compared to all other treatments. Similarly, feed efficiency (feed/gain) was improved (P < 0.004) for pigs fed RBSBB and RBS20 diets compared with pigs fed RBS5 or RBB2.5. For economics, income over feed cost was the greatest (P < 0.01) for the diet containing soybean meal and base mix compared to pigs fed the RBS20 diet, SBS5, and RBB2.5. In conclusion, feeding pigs a rice bran diet with base mix and soybean meal had similar growth performance and increased economic return compared to feeding a rice bran diet with high protein supplement. Use of soybean meal and base mix provides an opportunity for increased economic return for pig farmers.

Bifidobacterium longum-fermented rice bran and rice bran supplementation affects the gut microbiome and metabolome.

This study investigated gut microbiota composition along with food, host, and microbial derived metabolites in the colon and systemic circulation of healthy mice following dietary rice bran and fermented rice bran intake. Adult male BALB/c mice were fed a control diet or one of two experimental diets containing 10% w/w rice bran fermented by Bifidobacterium longum or 10% w/w non-fermented rice bran for 15 weeks. Metabolomics was performed on the study diets (food), the murine colon and whole blood. These were analysed in concert with 16S rRNA amplicon sequencing of faeces, caecum, and colon microbiomes. Principal components analysis of murine microbiota composition displayed marked separation between control and experimental diets, and between faecal and tissue (caecum and colon) microbiomes. Colon and caecal microbiomes in both experimental diet groups showed enrichment of Roseburia, Lachnospiraceae, and Clostridiales related amplicon sequence variants compared to control. Bacterial composition was largely similar between experimental diets. Metabolite profiling revealed 530 small molecules comprising of 39% amino acids and 21% lipids that had differential abundances across food, colon, and blood matrices, and statistically significant between the control, rice bran, and fermented rice bran groups. The amino acid metabolite, N-delta-acetylornithine, was notably increased by B. longum rice bran fermentation when compared to non-fermented rice bran in food, colon, and blood. These findings support that dietary intake of rice bran fermented with B. longum modulates multiple metabolic pathways important to the gut and overall health.

The overexpression of rice ACYL-CoA-BINDING PROTEIN2 increases grain size and bran oil content in transgenic rice.

As Oryza sativa (rice) seeds represent food for over three billion people worldwide, the identification of genes that enhance grain size and composition is much desired. Past reports have indicated that Arabidopsis thaliana acyl-CoA-binding proteins (ACBPs) are important in seed development but did not affect seed size. Herein, rice OsACBP2 was demonstrated not only to play a role in seed development and germination, but also to influence grain size. OsACBP2 mRNA accumulated in embryos and endosperm of germinating seeds in qRT-PCR analysis, while β-glucuronidase (GUS) assays on OsACBP2pro::GUS rice transformants showed GUS expression in embryos, as well as the scutellum and aleurone layer of germinating seeds. Deletion analysis of the OsACBP2 5'-flanking region revealed five copies of the seed cis-element, Skn-I-like motif (-1486/-1482, -956/-952, -939/-935, -826/-822, and -766/-762), and the removal of any adversely affected expression in seeds, thereby providing a molecular basis for OsACBP2 expression in seeds. When OsACBP2 function was investigated using osacbp2 mutants and transgenic rice overexpressing OsACBP2 (OsACBP2-OE), osacbp2 was retarded in germination, while OsACBP2-OEs performed better than the wild-type and vector-transformed controls, in germination, seedling growth, grain size and grain weight. Transmission electron microscopy of OsACBP2-OE mature seeds revealed an accumulation of oil bodies in the scutellum cells, while confocal laser scanning microscopy indicated oil accumulation in OsACBP2-OE aleurone tissues. Correspondingly, OsACBP2-OE seeds showed gain in triacylglycerols and long-chain fatty acids over the vector-transformed control. As dietary rice bran contains beneficial bioactive components, OsACBP2 appears to be a promising candidate for enriching seed nutritional value.

Effect of rice bran fermented with Saccharomyces cerevisiae and Lactobacillus plantarum on gut microbiome of mice fed high-sucrose diet.

To clarify the effect of rice bran (RB) and fermented RB (FRB) in a high-sucrose and low-dietary fibre diet on the gut microbiome, the in vitro bile acid-lowering capacity and caecal microbiota of ICR mice fed with 20% RB or FRB diets for two weeks were determined. The caecal microbiome was analysed by 16S rRNA gene amplicon sequencing. The in vitro bile acid-lowering capacity was high for FRB. In mouse experiments, triacylglycerol and total cholesterol were generally lower with FRB, although the faecal frequency was highest in mice fed with RB. The Shannon-Wiener and Simpson's indices for alpha-diversity in the microbiome of mice fed with RB and FRB, were higher than mice fed the control diet. At the phylum level in the caecal microbiome, Firmicutes and Bacteroidetes were high with FRB and RB, respectively. At the operational taxonomic unit level, some bacterial groups related to diabetes and gut toxicity, such as Lachnospiraceae and Enterorhabdus mucosicola, were high for RB but not for FRB diets. These results suggest that FRB, rather than RB, intake improve the intestinal environment and blood lipid condition.

Immunostimulatory and antifertility effects of neem (Azadirachta indica) leaf extract on common carp (Cyprinus carpio Linnaeu)

180-day feeding trial was conducted on the growth performance, feeding efficacy, immunological parameters and gonadal reproductive parameters of common carp, Cyprinus carpio fed with neem (Azadirachta indica) leaf extract (NLE) incorporated at different levels (0, 0.25, 0.5, 1.0, 1.5 and 2.0 g kg-1 feed) in rice bran and mustard meal (1:1 ratio) basal diet. The study revealed highest Net Weight Gain (NWG) in fish fed on NE 1.0 g kg. Results showed that the good growth response was obtained in fish fed with the NLE 1.0 g kg-1 feed and least in fish fed only on basal diet (without NLE). Whereas, higher gonado-somatic index (GSI), relative fecundity and ova diameter values were obtained from fish fed on basal diet while the least were observed from fish fed only on basal diet, while the least value was fish fed with NLE 1.0 g kg-1 feed. Fish fed with NLE 1.0 g kg-1 feed also showed significantly (p<0.05) enhanced phagocytic activity ratio, Phagocytic index, NBT activity, CAT, SOD, GPx and GR responses in comparison with fish fed only on basal diet, where as LPO is significantly decreased on being fed with NLE up to 1g kg-1 feed. The study indicated that incorporation of NLE in feed improved growth, delayed female gonadal maturation and could be used as a potential immunostimulant and to control the prolificbreeding of C. carpio in aquaculture.

Dietary Rice Bran Plays A Significant Role in the Hepatoprotective Effect in Hypercholesterolemic Rats

Cholesterol is obtained through biosynthesis and diet. When a level of cholesterol is above the normal level, this condition caused hypercholesterolemia. Long-term administration of synthetic chemical drugs can cause liver damage. Therefore, alternative natural medication is needed. One of the alternatives that can be used is the rice bran (RB), which contains antioxidant and crude fiber. This study is aimed at finding out the potential utilization of RB on total cholesterol level, liver enzyme as aspartate aminotransferase (AST) and alanine aminotransferase (ALT), malondialdehyde (MDA), and the changes in liver tissue. This study uses five groups of rats: the negative control group, positive control group, and three therapy groups with the dosage of 270 mg kg -1 , 540 mg kg -1 , and 810 mg kg -1 of body weight. This study shows that therapy using RB can significantly decrease the cholesterol level, AST, ALT, and MDA (p<0.01). The total cholesterol level is 21%, AST and ALT activities can be reduced to 54% and 64%, the level of MDA reduced to 79% and can repair the liver tissue. This study shows that RB can be effectively used as hepatoprotective in rats with hypercholesterolemia. Keywords: AST and ALT, Hypercholesterolemia, Rice Bran, Total Cholesterol Level.