Retinal Eccentricity Research Articles

Impact of Reference Center Choice on Adaptive Optics Imaging Cone Mosaic Analysis.

PurposeFoveal center marking is a key step in retinal image analysis. We investigated the discordance between the adaptive optics (AO) montage center (AMC) and the foveal pit center (FPC) and its implications for cone mosaic analysis using a commercial flood-illumination AO camera.MethodsThirty eyes of 30 individuals (including 15 healthy and 15 patients with rod–cone dystrophy) were included. Spectral-domain optical coherence tomography was used to determine the FPC, and flood-illumination AO imaging was performed with overlapping image frames to create an AO montage. The AMC was determined by averaging the (0,0) coordinates in the four paracentral overlapping AO image frames. Cone mosaic measurements at various retinal eccentricities were compared between corresponding retinal loci relative to the AMC or FPC.ResultsAMCs were located temporally to the FPCs in 14 of 15 eyes in both groups. The average AMC–FPC discordance was 0.85° among healthy controls and 0.33° among patients with rod-cone dystrophy (P < 0.05). The distance of the AMC from the FPC was a significant determinant of the cone density (β estimate = 218 cells/deg2/deg; 95% confidence interval [CI], 107–330; P < 0.001) and inter-cone distance (β estimate = 0.28 arcmin/deg; 95% CI, 0.15–0.40; P < 0.001), after adjustment for age, sex, axial length, spherical equivalent, eccentricity, and disease status.ConclusionsThere is a marked mismatch between the AMC and FPC in healthy eyes that may be modified by disease process such as rod–cone dystrophy. We recommend users of AO imaging systems carefully align the AO montage with a foveal anatomical landmark, such as the FPC, to ensure precise and reproducible localization of the eccentricities and regions of interest for cone mosaic analysis.

Effect of target contrast and divided attention on the useful field of view

Previous research has shown that there is a cost of dividing attention between the central and peripheral visual fields in a complex environment. However, it is not clear how stimulus factors, such as the contrast of the scene, affect the cost. The current study reports the results of two studies that address this question. In Experiment 1, temporal thresholds of the Useful Field of View (UFOV) tests were measured as a function of contrast and retinal eccentricity. The results showed that central-focused attention thresholds increased (i.e., performance decreased) as contrast decreased. Peripheral and divided attention task performance decreased as eccentricity increased. Surprisingly, peripheral and divided attention task performance were the best for medium rather than high contrast targets. The unexpected poorer performance under the high contrast condition might possibly be explained by the crowding effect. To test this possible explanation, in Experiment 2 the peripheral stimuli were simplified to minimize the potential crowding effect on peripheral target detection. The results showed that the unexpected effect of contrast on the cost of dividing attention could be accounted for by the crowding effect. When combined, the results from the two experiments suggest that the cost of dividing attention between central and peripheral targets is more pronounced for objects at greater eccentricity under lower contrast conditions, consistent with a tunnel effect. The implications of this finding are discussed in the paper.

Asymmetric Peripheral Refraction Profile in Myopes along the Horizontal Meridian.

The investigation of peripheral refraction profiles in Indian myopes showed relative peripheral hyperopic refraction in temporal retina and possible dominant role of hyperopic defocus signals from temporal retina in the development of myopia. Considering that the peripheral refraction profiles were extensively reported to be associated with the central refractive error and vary among different ethnicities, we investigated the peripheral refraction profiles in Indians. A total of 161 participants aged between 18 and 33 years were included in the study. All of the eligible participants underwent a comprehensive eye examination. Central and peripheral refractions were determined using an open-field autorefractor in 10° intervals up to ±30° in the horizontal meridian, and in 5° intervals up to ±15° in the vertical meridian. Axial length and central corneal radius were measured using a non-contact optical biometer. Peripheral refraction was compared between the different refractive error groups and myopic subgroups. Myopes showed a significant asymmetrical peripheral refraction profile along horizontal meridian with relative peripheral myopia at nasal 30° and relative peripheral hyperopia at temporal 30° (mean ± standard error at N30°: -0.37 ± 0.13 D vs. T30°: +0.56 ± 0.11 D, P < .05). Emmetropes and hyperopes showed relative peripheral myopia both in nasal and temporal eccentricities. Relative peripheral refraction was significantly different between the refractive groups and myopic subgroups along the temporal retinal eccentricities only (P < .05). Along the vertical meridian, relative peripheral myopia was seen among the three refractive error groups (P < .05). J0 and J45 significantly changed with retinal eccentricity along both the meridians in all the refractive error groups (P < .05). Myopes showed an asymmetric type of peripheral refraction with relative hyperopic defocus in temporal retina and myopic defocus in the nasal retina. Possible role of retinal hyperopic defocus along temporal retina in myopiogenesis needs to be explored.

On the cortical mapping function – Visual space, cortical space, and crowding

The retino-cortical visual pathway is retinotopically organized: Neighbourhood relationships on the retina are preserved in the mapping. Size relationships in that mapping are also highly regular: The size of a patch in the visual field that maps onto a cortical patch of fixed size follows, along any radius and over a wide range, simply a linear function with retinal eccentricity. As a consequence, the mapping of retinal to cortical locations follows a logarithmic function along that radius. While this has already been shown by Fischer (1973, Vision Research, 13, 2113–2120), the link between the linear function – which describes the local behaviour by the cortical magnification factor M – and the logarithmic location function for the global behaviour, has never been made explicit. The present paper provides such a link as a set of ready-to-use equations using Levi and Klein’s E2 nomenclature, and examples for their validity and applicability in the mapping literature are discussed. The equations allow estimating M in the retinotopic centre; values thus derived from the literature show enormous, hitherto unnoticed, variability. A new structural parameter, d2, is proposed to characterize the cortical map, as a counterpart to E2; it shows much more stability. One pitfall is discussed and spelt out, namely the common myth that a pure logarithmic function, without constant term, will give an adequate map. The correct equations are finally extended to describe the cortical map of Bouma’s law on visual crowding. The result contradicts recent suggestions that critical crowding distance corresponds to constant cortical distance.

Binocularly Asymmetric Crowding in Glaucoma and a Lack of Binocular Summation in Crowding.

PurposeGlaucoma is associated with progressive loss of retinal ganglion cells. Here we investigated the impact of glaucomatous damage on monocular and binocular crowding in parafoveal vision. We also examined the binocular summation of crowding to see if crowding is alleviated under binocular viewing.MethodsThe study design included 40 individuals with glaucoma and 24 age-similar normal cohorts. For each subject, the magnitude of crowding was determined by the extent of crowding zone. Crowding zone measurements were made binocularly in parafoveal vision (i.e., at 2° and 4° retinal eccentricities) visual field. For a subgroup of glaucoma subjects (n = 17), crowding zone was also measured monocularly for each eye.ResultsOur results showed that, compared with normal cohorts, individuals with glaucoma exhibited significantly larger crowding—enlargement of crowding zone (an increase by 21%; P < 0.01). Moreover, we also observed a lack of binocular summation (i.e., a binocular ratio of 1): binocular crowding was determined by the better eye. Hence, our results did not provide evidence supporting binocular summation of crowding in glaucomatous vision.ConclusionsOur findings show that crowding is exacerbated in parafoveal vision in glaucoma and binocularly asymmetric glaucoma seems to induce binocularly asymmetric crowding. Furthermore, the lack of binocular summation for crowding observed in glaucomatous vision combined with the lack of binocular summation reported in a previous study on normal healthy vision support the view that crowding may start in the early stages of visual processing, at least before the process of binocular integration takes place.

Relationship Between Peripheral Refraction in Different Retinal Regions and Myopia Development of Young Chinese People.

ObjectiveTo observe the associations between regional peripheral refraction and myopia development in young Chinese people.MethodsTwo hundred and forty-one young adult subjects (21 emmetropes, 88 low myopes, 94 moderate myopes, and 38 high myopes) aged 18–28 years were included, and only the right eyes were tested. Eye biometrics were measured before pupil dilation using the Lenstar. Relative peripheral refractive errors (RPRE) were measured after pupil dilation using multispectral refractive topography (MRT), at nine retinal eccentricities: 0–5, 5–10, 10–15, 15–20, 20–25, 25–30, 30–35, 35–40, and 40–45 degrees.ResultsIn this study, RPRE increased with eccentricity, and it shows a growing trend with the increase of the degree of myopia among emmetropia, low myopia and moderate myopia groups, and RPRE varied with myopia severity at eccentricities between 20 and 35 degrees only. In addition, axial length (AL) and RPRE were positively correlated between 20 and 45 degrees, and AL was an independent risk factor for RPRE between 20 and 35 degrees.ConclusionThese findings indicate that the eccentricities between 20 and 35 degrees RPRE may be closely related to refractive development and eye growth in young Chinese people.

A BCI-Based Study on the Relationship Between the SSVEP and Retinal Eccentricity in Overt and Covert Attention.

Covert attention aids us in monitoring the environment and optimizing performance in visual tasks. Past behavioral studies have shown that covert attention can enhance spatial resolution. However, electroencephalography (EEG) activity related to neural processing between central and peripheral vision has not been systematically investigated. Here, we conducted an EEG study with 25 subjects who performed covert attentional tasks at different retinal eccentricities ranging from 0.75° to 13.90°, as well as tasks involving overt attention and no attention. EEG signals were recorded with a single stimulus frequency to evoke steady-state visual evoked potentials (SSVEPs) for attention evaluation. We found that the SSVEP response in fixating at the attended location was generally negatively correlated with stimulus eccentricity as characterized by Euclidean distance or horizontal and vertical distance. Moreover, more pronounced characteristics of SSVEP analysis were also acquired in overt attention than in covert attention. Furthermore, offline classification of overt attention, covert attention, and no attention yielded an average accuracy of 91.42%. This work contributes to our understanding of the SSVEP representation of attention in humans and may also lead to brain-computer interfaces (BCIs) that allow people to communicate with choices simply by shifting their attention to them.

Goji Berry Intake Increases Macular Pigment Optical Density in Healthy Adults: A Randomized Pilot Trial.

Age-related macular degeneration (AMD) is the third leading cause of blindness worldwide. Macular pigment optical density (MPOD), a biomarker for AMD, is a non-invasive measure to assess risk. The macula xanthophyll pigments lutein (L) and zeaxanthin (Z) protect against blue light and provide oxidant defense, which can be indexed by MPOD. This study examined the effects of Z-rich goji berry intake on MPOD and skin carotenoids in healthy individuals. A randomized, unmasked, parallel-arm study was conducted with 27 participants, aged 45–65, who consumed either 28 g of goji berries or a supplement containing 6 mg L and 4 mg Z (LZ), five times weekly for 90 days. After 90 days, MPOD was significantly increased in the goji berry group at 0.25 and 1.75 retinal eccentricities (p = 0.029 and p = 0.044, respectively), while no changes were noted in the LZ group. Skin carotenoids were significantly increased in the goji berry group at day 45 (p = 0.025) and day 90 (p = 0.006), but not in the LZ group. Regular intake of goji berries in a healthy middle-aged population increases MPOD may help prevent or delay the development of AMD.

Implementation of the Frequency Scatter Index in Clinical Commercially Available Double-pass Systems

ABSTRACTA previous work has reported a methodology to quantify intraocular scattering using a high sensitivity double-pass instrument with a robust index, the frequency scatter index. The purpose of our study was to evaluate an adaptation of the frequency scatter index for use in clinical double-pass systems. A prospective observational study was carried out in a group of patients with nuclear cataracts (n = 52) and in a control group (n = 11) using conventional double-pass systems. The frequency scatter index and the objective scatter index were used to assess the scattering. The Spearman coefficient was calculated to assess the correlation between both indexes, obtained from the double-pass images. Simultaneous measurements were performed with a double-pass and with a Hartmann-Shack wavefront sensor in the control group. The root-mean-square wavefront error and the full width at half maximum of the double-pass image were used to quantify the residual aberrations introduced by the variations in pupil size and retinal eccentricity. Measurement in eyes with different grades of cataracts shows a strong correlation (ρ = 0.929, p < .0001) between the frequency scatter index and the objective scatter index. A certain degree of correlation was observed between the objective scatter index and the root-mean-square and between the objective scatter index and the full width at half maximum, both for measurements with a different pupillary diameter and with a different retinal eccentricity (p < .05). No relationship was observed between the frequency scatter index and the root-mean-square or between the frequency scatter index and the full width at half maximum (p > .05). We have introduced and evaluated an adaptation of a methodology proposed recently for the measurement of intraocular scattering using the double-pass technique with a robust index, which is less affected by ocular aberrations. The frequency scatter index can be applied to conventional double-pass instruments available in clinical environments.

Local Progression Kinetics of Geographic Atrophy Depends Upon the Border Location.

PurposeTo assess the influence of lesion morphology and location on geographic atrophy (GA) growth rate.MethodsWe manually delineated GA on color fundus photographs of 237 eyes in the Age-Related Eye Disease Study. We calculated local border expansion rate (BER) as the linear distance that a point on the GA border traveled over 1 year based on a Euclidean distance map. Eye-specific BER was defined as the mean local BER of all points on the GA border in an eye. The percentage area affected by GA was defined as the GA area divided by the total retinal area in the region.ResultsGA enlarged 1.51 ± 1.96 mm2 in area and 0.13 ± 0.11 mm in distance over 1 year. The GA area growth rate (mm2/y) was associated with the baseline GA area (P < 0.001), perimeter (P < 0.001), lesion number (P < 0.001), and circularity index (P < 0.001); in contrast, eye-specific BER (mm/y) was not significantly associated with any of these factors. As the retinal eccentricity increased from 0 to 3.5 mm, the local BER increased from 0.10 to 0.24 mm/y (P < 0.001); in contrast, the percentage of area affected by GA decreased from 49.3% to 2.3%.ConclusionsUsing distance-based measurements allows GA progression evaluation without significant confounding effects from baseline GA morphology. Local GA progression rates increased as a function of retinal eccentricity within the macula which is opposite of the trend for GA distribution, suggesting that GA initiation and enlargement may be mediated by different biological processes.

The Relationship Between Perifoveal L-Cone Isolating Visual Acuity and Cone Photoreceptor Spacing-Understanding the Transition Between Healthy Aging and Early AMD.

Background: Age-related macular degeneration (AMD) is a multifactorial degenerative disorder that can lead to irreversible loss of visual function, with aging being the prime risk factor. However, knowledge about the transition between healthy aging and early AMD is limited. We aimed to examine the relationship between psychophysical measures of perifoveal L-cone acuity and cone photoreceptor structure in healthy aging and early AMD.Methods and Results: Thirty-nine healthy participants, 10 with early AMD and 29 healthy controls were included in the study. Multimodal high-resolution retinal images were obtained with adaptive-optics scanning-light ophthalmoscopy (AOSLO), optical-coherence tomography (OCT), and color fundus photographs. At 5 degrees retinal eccentricity, perifoveal L-cone isolating letter acuity was measured with psychophysics, cone inner segment and outer segment lengths were measured using OCT, while cone density, spacing, and mosaic regularity were measured using AOSLO. The Nyquist sampling limit of cone mosaic (Nc) was calculated for each participant. Both L-cone acuity and photoreceptor inner segment length declined with age, but there was no association between cone density nor outer segment length and age. A multiple regression showed that 56% of the variation in log L-cone acuity was accounted for by Nc when age was taken into account. Six AMD participants with low risk of progression were well within confidence limits, while two with medium-to-severe risk of progression were outliers. The observable difference in cone structure between healthy aging and early AMD was a significant shortening of cone outer segments.Conclusion: The results underscore the resilience of cone structure with age, with perifoveal functional changes preceding detectable changes in the cone photoreceptor mosaic. L-cone acuity is a sensitive measure for assessing age-related decline in this region. The transition between healthy aging of cone structures and changes in cone structures secondary to early AMD relates to outer segment shortening.

Medical image quality metrics for foveated model observers.

Purpose: A recently proposed model observer mimics the foveated nature of the human visual system by processing the entire image with varying spatial detail, executing eye movements, and scrolling through slices. The model can predict how human search performance changes with signal type and modality (2D versus 3D), yet its implementation is computationally expensive and time-consuming. Here, we evaluate various image quality metrics using extensions of the classic index of detectability expression and assess foveated model observers for search tasks. Approach: We evaluated foveated extensions of a channelized Hotelling and nonprewhitening matched filter model with an eye filter. The proposed methods involve calculating a model index of detectability ( ) for each retinal eccentricity and combining these with a weighting function into a single detectability metric. We assessed different versions of the weighting function that varied in the required measurements of the human observers' search (no measurements, eye movement patterns, size of the image, and median search times). Results: We show that the index of detectability across eccentricities weighted using the eye movement patterns of observers best predicted human performance in 2D versus 3D search performance for a small microcalcification-like signal and a larger mass-like. The metric with a weighting function based on median search times was the second best predicting human results. Conclusions: The findings provide a set of model observer tools to evaluate image quality in the early stages of imaging system evaluation or design without implementing the more computationally complex foveated search model.

Retinal Responses to Simulated Optical Blur Using a Novel Dead Leaves ERG Stimulus.

PurposeThe purpose of this study was to evaluate retinal responses to different types and magnitudes of simulated optical blur presented at specific retinal eccentricities using naturalistic images.MethodsElectroretinograms (ERGs) were recorded from 27 adults using 30-degree dead leaves naturalistic images, digitally blurred with one of three types of optical blur (defocus, astigmatism, and spherical aberrations), and one of three magnitudes (0.1, 0.3, or 0.5 µm) of blur. Digitally computed blur was applied to the entire image, or on an area outside the central 6 degrees or 12 degrees of retinal eccentricity.ResultsERGs were significantly affected by blur type, magnitude, and retinal eccentricity. ERGs were differentially affected by defocus and spherical aberrations; however, astigmatism had no effect on the ERGs. When blur was applied only beyond the central 12 degrees eccentricity, the ERGs were unaffected. However, when blur was applied outside the central 6 degrees, the ERG responses were significantly reduced and were no different from the ERGs recorded with entirely blurred images.ConclusionsBlur type, magnitude, and location all affect the retinal responses. Our data indicate that the retinal area between 6 and 12 degrees eccentricity has the largest effect on the retinal responses to blur. In addition, certain optical blur types appear to have a more detrimental effect on the ERGs than others. These results cannot be solely explained by changes to image contrast and spatial frequency content, suggesting that retinal neurons might be sensitive to spatial cues in order to differentiate between different blur types.

Morphological properties of the axon initial segment-like process of AII amacrine cells in the rat retina.

Signal processing within the retina is generally mediated by graded potentials, whereas output is conveyed by action potentials transmitted along optic nerve axons. Among retinal neurons, amacrine cells seem to be an exception to this general rule, as several types generate voltage-gated Na+ (Nav ) channel-dependent action potentials. The AII, a narrow-field, bistratified axon-less amacrine cell found in mammalian retinas, displays a unique process that resembles an axon initial segment (AIS), with expression of Nav channels colocalized with the cytoskeletal protein ankyrin-G, and generates action potentials. As the role of spiking in AIIs is uncertain, we hypothesized that the morphological properties of the AIS-like process could provide information relevant for its functional importance, including potential pre- and/or postsynaptic connectivity. For morphological analysis, we injected AII amacrine cells in slices with fluorescent dye and immunolabeled the slices for ankyrin-G. Subsequently, this enabled us to reliably identify AII-type processes among ankyrin-G-labeled processes in wholemount retina. We systematically analyzed the laminar localization, spatial orientation, and distribution of the AIS-like processes as a function of retinal eccentricity. In the horizontal plane, the processes displayed no preferred orientation and terminal endings were randomly distributed. In the vertical plane, the processes displayed a horizontal preference, but also ascended and descended into the inner nuclear layer and proximal inner plexiform layer, respectively. These results suggest that the AII amacrine AIS-like process is unlikely to take part in conventional synaptic connections, but may instead be adapted to respond to volume neurotransmission by means of extrasynaptic receptors.

Macular Pigment Response to Lutein, Zeaxanthin, and Meso-zeaxanthin Supplementation in Open-Angle Glaucoma: A Randomized Controlled Trial

PurposeTo evaluate macular pigment response to carotenoid supplementation in glaucomatous eyes.DesignDouble-masked, randomized, placebo-controlled clinical trial, the European Nutrition in Glaucoma Management Study (ClinicalTrials.gov identifier, NCT04460365).ParticipantsSixty-two participants (38 men, 24 women) with a diagnosis of open-angle glaucoma were enrolled. Forty-two were randomized to receive the active supplement, 20 participants were allocated to placebo.MethodsMacular pigment optical density (MPOD) was measured by autofluorescence using the Heidelberg Spectralis scanning laser ophthalmoscope. Macular pigment optical density volume within the central 6° of retinal eccentricity as well as MPOD at 0.23°, 0.51°, 0.74°, and 1.02° were recorded at baseline and at 6-month intervals over 18 months. Visual function was assessed using visual acuity, mesopic and photopic contrast sensitivity under glare conditions, photo stress recovery time, microperimetry, and Glaucoma Activities Limitation 9 questionnaire. Advanced glaucoma module scans of retinal nerve fiber layer thickness and ganglion cell complex thickness over the central 6° of retinal eccentricity also were completed at each study visit.Main Outcome MeasuresChange in MPOD after supplementation with 10 mg lutein, 2 mg zeaxanthin, and 10 mg meso-zeaxanthin or placebo over 18 months.ResultsA mixed-model repeated measures analysis of variance revealed a statistically significant increase in MPOD volume (significant time effect: F(3,111) = 89.31, mean square error (MSE) = 1656.9; P < 0.01). Post hoc t tests revealed a significant difference in MPOD volume at each study visit for the treatment group (P < 0.01 for all), but no change in the placebo group (P > 0.05 for all). A statistically significant increase in mesopic contrast sensitivity under glare conditions was noted at 18 months in the treatment group, but not placebo. No other structural or functional changes were observed. No serious adverse events were noted during the trial.ConclusionsMacular pigment can be augmented in glaucomatous eyes by supplementation with a formulation containing the carotenoids lutein, zeaxanthin, and meso-zeaxanthin. The greatest relative benefit was observed in those with the lowest baseline levels, but increases were noted across all participants and each retinal eccentricity. The potential benefits of MP augmentation for macular health in glaucoma merit further long-term evaluation.

Retinal Differential Light Sensitivity Variation Across the Macula in Healthy Subjects: Importance of Cone Separation and Loci Eccentricity.

PurposeMicroperimetry measures differential light sensitivity (DLS) at specific retinal locations. The aim of this study is to examine the variation in DLS across the macula and the contribution to this variation of cone distribution metrics and retinal eccentricity.MethodsForty healthy eyes of 40 subjects were examined by microperimetry (MAIA) and adaptive optics imaging (rtx1). Retinal DLS was measured using the grid patterns: foveal (2°–3°), macular (3°–7°), and meridional (2°–8° on horizontal and vertical meridians). Cone density (CD), distribution regularity, and intercone distance (ICD) were calculated at the respective test loci coordinates. Linear mixed-effects regression was used to examine the association between cone distribution metrics and loci eccentricity, and retinal DLS.ResultsAn eccentricity-dependent reduction in DLS was observed on all MAIA grids, which was greatest at the foveal-parafoveal junction (2°–3°) (−0.58 dB per degree, 95% confidence interval [CI]; −0.91 to −0.24 dB, P < 0.01). Retinal DLS across the meridional grid changed significantly with each 1000 cells/deg2 change in CD (0.85 dB, 95% CI; 0.10 to 1.61 dB, P = 0.03), but not with each arcmin change in ICD (1.36 dB, 95% CI; −2.93 to 0.20 dB, P = 0.09).ConclusionsWe demonstrate significant variation in DLS across the macula. Topographical change in cone separation is an important determinant of the variation in DLS at the foveal-parafoveal junction. We caution the extrapolation of changes in DLS measurements to cone distribution because the relationship between these variables is complex.Translational RelevanceCone density is an independent determinant of DLS in the foveal-parafoveal junction in healthy eyes.

Do myopes have deficits in peripheral flicker sensitivity?

PurposeSignals from the peripheral retina are important for myopia development. Unlike temporal vision, deficits in peripheral spatial visual functions of myopes have been investigated previously. This study investigated temporal contrast thresholds in emmetropes and myopes at different retinal eccentricities. MethodsForty-four young adults (mean age 23 ± 3 years) including 21 emmetropes (Spherical Equivalent (SE): +0.01 ± 0.30D) and 23 myopes (SE: -3.98 ± 2.41D) participated in this prospective study. Flicker modulation thresholds (FMT) were determined monocularly (right eye) for 15 Hz flicker stimulus at 0°, nasal (23°, 10°) and temporal (-23°, -10°) retinal eccentricities along the horizontal meridian. FMTs were measured psychophysically using 5-adaptive interleaved staircases and threshold was taken as the average of the last 6 reversals. ResultsIn both the groups (emmetropes and myopes), there was a naso-temporal asymmetry in FMTs with higher thresholds in the far temporal retina (Median; Interquartile range: 40.97%; 17.06) than the nasal retina (28.07%; 9.36) (p < 0.001). Flicker modulation thresholds were significantly higher in myopes (30.58%; 12.15) compared to emmetropes (26.77%; 7.74; p = 0.04) at far nasal retina (23°), while at other eccentricities there was no effect (p > 0.05). Further sub-analysis revealed only high myopes (34.48 %, 21.9) showed significantly higher FMT compared to emmetropes (26.77%; 7.74; p = 0.04). ConclusionGreater FMTs were seen in high myopes than that of emmetropes in the nasal retina. Further studies exploring the structural aspects of the myopic eye with FMT would provide a better understanding of role of flicker sensitivity in myopiogenesis.

Evaluation of photoreceptor function in inherited retinal diseases using rod- and cone-enhanced flicker stimuli.

Clinical assessment of rod and cone photoreceptor sensitivity often involves the use of extended dark adaptation times to minimise cone involvement or the use of bright adapting backgrounds to saturate rods. In this study we examine a new rod/cone sensitivity test, which requires minimal dark adaptation. The aim was to establish whether rod/cone sensitivity losses could be measured reliably in patients with retinal diseases that selectively affect rods or cones when compared to age-matched subjects with normal vision. Flicker modulation thresholds (FMTs) were measured psychophysically, using cone- and rod-enhanced stimuli located centrally, and in four quadrants, at 5° retinal eccentricity in 20 patients (age range: 10-41years) with cone-dominated (Stargardt's disease or macular dystrophy; n=13) and rod-dominated (retinitis pigmentosa; n=7) disease. These data were compared against age-matched normals tested with identical stimuli. Across all retinal locations, cone FMTs in cone-dominated diseases (Median±IQR: 32.32±28.15% for central location) were greater than a majority (83%; 49/59) of corresponding rod FMTs (18.7±3.29%; p=0.05) and cone FMTs of controls (4.24±2.00%). Similarly, rod FMTs in rod-dominant disease (14.99±22.58%) were greater than a majority (88%; 29/39) of the corresponding cone FMTs (9.09±10.33%) (p=0.13) and rod FMT of controls (6.80±2.60 %). Cone-specific deficits were larger than rod-specific deficits in cone-dominated diseases, and vice versa in rod-dominated disease. These results suggest that the new method of assessing photoreceptor sensitivity has potential application in detecting specific rod/cone losses without the need for dark adaptation.

Investigating Visual Crowding of Objects in Complex Real-World Scenes.

Visual crowding, the impairment of object recognition in peripheral vision due to flanking objects, has generally been studied using simple stimuli on blank backgrounds. While crowding is widely assumed to occur in natural scenes, it has not been shown rigorously yet. Given that scene contexts can facilitate object recognition, crowding effects may be dampened in real-world scenes. Therefore, this study investigated crowding using objects in computer-generated real-world scenes. In two experiments, target objects were presented with four flanker objects placed uniformly around the target. Previous research indicates that crowding occurs when the distance between the target and flanker is approximately less than half the retinal eccentricity of the target. In each image, the spacing between the target and flanker objects was varied considerably above or below the standard (0.5) threshold to either suppress or facilitate the crowding effect. Experiment 1 cued the target location and then briefly flashed the scene image before participants could move their eyes. Participants then selected the target object’s category from a 15-alternative forced choice response set (including all objects shown in the scene). Experiment 2 used eye tracking to ensure participants were centrally fixating at the beginning of each trial and showed the image for the duration of the participant’s fixation. Both experiments found object recognition accuracy decreased with smaller spacing between targets and flanker objects. Thus, this study rigorously shows crowding of objects in semantically consistent real-world scenes.

What is visible across the visual field?

It is sometimes claimed that because the resolution and sensitivity of visual perception are better in the fovea than in the periphery, peripheral vision cannot support the same kinds of colour and sharpness percepts as foveal vision. The fact that a scene nevertheless seems colourful and sharp throughout the visual field then poses a puzzle. In this study, I use a detailed model of human spatial vision to estimate the visibility of certain properties of natural scenes, including aspects of colourfulness, sharpness, and blurriness, across the visual field. The model is constructed to reproduce basic aspects of human contrast and colour sensitivity over a range of retinal eccentricities. I apply the model to colourful, complex natural scene images, and estimate the degree to which colour and edge information are present in the model’s representation of the scenes. I find that, aside from the intrinsic drift in the spatial scale of the representation, there are not large qualitative differences between foveal and peripheral representations of ‘colourfulness’ and ‘sharpness’.