The aim of this research is to determine whether palaeoparasitological analysis of mineralized concretions found within a stone vessel from the Roman city of Viminacium can shed light on its past use. The ruins of Viminacium, the capital of the Roman province of Moesia Superior, are situated 90 km southeast of the Belgrade in Serbia, and about 12 km from the town of Kostolac in the vicinity of villages Stari Kostolac and Drmno. It was established as a military camp in the first century CE with a legion permanently stationed there. The stone vessel was discovered during systematic archaeological excavations of the public bath complex with a public toilet near the amphitheater in Viminacium. The finding is dated by relative chronology in the second half of the third century. The vessel is topologically defined as a mortarium according to shape and size. Mineralized deposits were preserved inside the vessel rim, sides, and base. Microscopic analysis of the mineralized deposit revealed eggs of intestinal nematode roundworms (most likely Ascaris sp., but possibly Pterygodermatites sp.) from the sides and base of the vessel. This is the first time that parasite eggs have been identified from concretions inside a Roman stone vessel, and have strong parallels with a previous finding of parasite eggs in mineralized deposits from a ceramic chamber pot from late antique Sicily. The finding suggests the possible use, or secondary use, and function of the stone vessel as a sanitary item (such as storing wiping implements) for personal hygiene in the public bath of Viminacium. Further paleoparasitological research requires a systematic analysis of calcified deposits from Roman period stone vessels for more clear insights into their function, use and reuse.