The optimization of floating bifacial solar panels (FBS PV) in tropical freshwater systems is explored by employing response surface methodology (RSM) and central composite design (CCD). Previous studies have yet to explore the long-term durability, environmental impact, economic viability, and performance of FBS PV systems under various climatic conditions. This study addresses this gap by focusing on panel height, water depth, and tilt angle to improve performance. The quadratic model reveals significant non-linear relationships impacting FBS PV power generation with freshwater cooling. Our models demonstrate high explanatory power, with R-squared values of 0.9831 for output power and 0.9900 for Bi-Facial gain. Experimental validation using conventional white surface (CWS) and proposed freshwater surface (PFS) indicates notable improvements in power generation, achieving a 4.34 to 4.86% gain in bifacial efficiency across various irradiation levels. Under 950 W/m2 irradiation, freshwater cooling achieves a 3.19% higher bifacial gain compared to CWS cooling. Panel temperature analysis shows consistent reductions with freshwater cooling, ranging from 1.43 to 2.72°C, enhancing overall efficiency and longevity. This research highlights the potential of freshwater cooling in optimizing bifacial solar systems, offering actionable insights for sustainable energy solutions in tropical regions.