Improving the quality of restoration of the destroyed coronal part of the teeth by orthopaedic methods has led to significant scientific and clinical interest and has been discussed in numerous reports, but the issues on reparative regeneration and restoration of the gingival margin relief in the area of the anterior group of teeth after reconstructive orthodontic measures in the case of restoration of destroyed tooth crowns and deep subgingival carious destruction have not been sufficiently studied yet. The purpose of this study was to investigate the microbiological peculiarities of applying a clinical diagnostic complex of manipulations designed to restore anterior teeth with cast posts to provide the grounds for long-term favourable outcomes of further orthopaedic treatment in order to obtain aesthetic appearance, taking into account the state of the surrounding soft tissues. To carry out high-quality preparation of periodontal tissues around the abutment tooth, patients underwent gingivectomy using a scalpel with a disposable blade, diode laser electrocoagulation and coagulation. The effectiveness of each method of the preparation of the marginal gingiva was evaluated by the findings of clinical and microbiological studies. We have found out all three tested methods of forming a new gum relief in the root area of a preserved tooth can significantly improve the qualitative and quantitative composition of the microflora in the stump area. A significant decrease in the frequency of biotope colonization by pathogenic and transient microorganisms and the improvement of the species composition in the local oral microbiocenosis can be achieved by cutting the hypertrophied epithelial edge of the cervical area with a microsurgical scalpel and using laser coagulation. Clinical and microbiological studies have confirmed the effectiveness of the clinical diagnostic complex of techniques for preparing the marginal gum around the root of the tooth, which is planned for orthodontic treatment with a post and an inlay. The use of a diode laser has been found to show the highest efficiency compared to other presented methods. All of the above techniques can be used in dental practice as methods of choice.