Red Betel Leaf Research Articles

Effect of Microencapsulation Techniques on Physical and Chemical Characteristics of Functional Beverage Based on Red Betel Leaf Extract (Piper crocatum)

Functional drinks based on red betel leaf extract have antioxidant activity, but they still have a bitter taste. This study aims to determine the effect of microencapsulation on phenol content, antioxidant activity, and sensory quality of functional drinks based on betel leaf extract. Microencapsulation of functional drinks was made using maltodextrin coatings with concentrations of 10% and 20%. Antioxidant activity was tested by the CUPRAC method. The ready to drink (RTD) functional drink has a total phenolic content and antioxidant activity of 782.30 ± 2.54 mg GAE/g and 1660.19 ± 31.67 µmol Tr/g, respectively. These values are higher than microencapsulated functional drinks with maltodextrin (MM). The microencapsulated functional drink with 10% maltodextrin coating (MM10) is the chosen formulation since it has the smallest particle size (1.283 µm), total phenolic content of 12.90 ± 0.01 mg GAE/g and antioxidant activity of 189.41 ± 1.88 µmol Tr/g. Microencapsulated functional drinks provide sensory quality that is not significantly different (p <0.05) from ready to drink (RTD) drinks.

Derajat Keasaman (pH) Saliva Meningkat Setelah Berkumur Rebusan Daun Sirih Merah (Paper Crocatum) Konsentrasi 10% pada Lansia Penderita Diabetes Melitus Tipe 2

Dental caries is the major dental and oral health problems in Indonesia and world wide. Alteration in the saliva acidity level (pH) in the oral environment plays an important role in dental caries. Red betel leaf (piper crocatum) is a herbal plant that commonly found in Indonesia. Red betel leaf contains alkaloid, flavonoid, tannin and essential oil which have antibackterial activities. Objectives : The aim of this study was to determine the acidity change (pH) of saliva in elderly people with diabetes mellitus type 2 after gargling of 10% boiled red betel leaves.Methods : the subjects of this study were 30 persons who divided randomly into two groups i.e treatment group and control group. Each group consisted of 15 persons. In the treatment group, the subjects gargled using 10% boiled red betel leaves, while in the control group the subject gargled using aquades. The saliva was collected on each time treated and followed by pH measuring. The data analysis was conducted by Independent t-test statistic (p≤0,05).Results : the results showed that there was an effect of increasing salivary acidity (pH) of saliva after gargling boiled of red betel leaf (Piper crocatum) in elderly people with diabetes mellitus type 2. Conclusion : The change of acidity level (pH) of saliva was increased significantly at 5th to 15th minute from 5.33 to 6.67 after gargling boiled red betel leaves concentration of 10%. The acidity level range stated above indicates a normal. Key words : pH of saliva, red betel leaves, elderly, diabetes mellitus type 2

Pemanfaatan Herbal Dalam Penyembuhan Luka Perineum

Periode masa nifas adalah masa kembalinya organ reproduksi seperti keadaan sebelum hamil dalam waktu enam minggu setelah melahirkan. Infeksi nifas merupakan masalah yang sering terjadi pada masa nifas, salah satunya disebabkan oleh luka jalan lahir. Penyembuhan luka dapat dilakukan secara medis konvensional atau dengan terapi komplementer. Terapi komplementer juga dikenal dengan obat tradisional atau obat herbal untuk kesehatan dan keefektifannya diakui melalui beberapa jenis penelitian. Studi ini merupakan suatu tinjauan literatur yang mencoba menggali pemanfaatan herbal dalam penyembuhan luka perineum. Metode literatur dalam artikel ini yaitu dengan mengumpulkan jurnal terkait tanaman yang digunakan untuk menyembuhkan luka perineum. Hasil review dari 5 penelitian menunjukkan beberapa tanaman seperti lidah buaya, kayu manis, daun sirih merah, daun pegagan, dan daun teh hijau dapat digunakan sebagai alternatif penyembuhan luka perineum.

Effect of Red Betel Leaf Extract (Piper crocatum) on Digestive Organ and Ileal Villi Developments in Broiler

Effect of Red Betel Leaf Extract (Piper crocatum) on Digestive Organ and Ileal Villi Developments in Broiler

KOMPRES KUBIS DAN SIRIH MERAH EFEKTIF MENURUNKAN DERAJAT PEMBENGKAKAN PAYUDARA IBU POSTPARTUM

As a mother will certainly give all the best, including exclusive breastfeeding for her beloved baby. One of the failures in breastfeeding is due to breast swelling. Cabbage leaves contain sinigrin (allylisothiocyanate) rapine, mustardoil, magnesium, oxylate, sulfur. A red betel leaf contains flavonoids, polevenolad, tannins, and essential oils. Empirically these substances have the effect of disappearing pain and swelling. The purpose of this study was to determine the effectiveness of cabbage and red betel leaf compresses on the degree of postpartum breast swelling.The research method is quasy experimental study with pretest-posttest with control group design. The study population was mothers who gave birth in March to April 2019 in the working area of Mungkid Magelang Public Health Center with a total of 45 respondents. Respondents were divided into 3 groups: 15 postpartum mothers with cabbage compress intervention group, 15 postpartum mothers with red betel leaf compress intervention group and 15 postpartum mothers with control group. The sampling technique is total sampling. Analyze using the Wilcoxon statistical test.Theresults showed that there were any differences in the scale of swelling before and after the intervention in the experiment and control groups. Cabbage compress with <p = 0.001, Red Betel Leaf Compress with p = 0.014 and Control Group with p = 0.025. Cabbage compress is the most effective in reducing the degree of postpartum breast swelling. It is recommended that postpartum mothers with breast swelling can apply cabbage compress as one of the technique to reduce the degree of breast swelling. For health workers, especially midwives are expected to teach cabbage compress techniques to postpartum mothers as one of the non-pharmacological therapies to reduce the degree of postpartum breast swelling.

Efektivitas Rebusan Daun Sirih Merah (Piper Crocatum) Terhadap Penurunan Kadar Gula Darah Penderita Diabetes Mellitus

Diabetes mellitus is a metabolic disease that arises in a person due to an increase in blood glucose levels above normal values. WHO Data (2016) showed the case of diabetes in Indonesia has an increased tendency of 5.7% in 2007 to 6.9% in 2016. This Data also showed that diabetes with complications is the third highest cause of death in Indonesia. Referring to national prevalency, the Province of West Sumatra has total Diabetes Mellitus as much as 1.3% which is in the order of 14 of the 33 provinces in Indonesia. Incidence of diabetes mellitus disease in Padang City is in the sixth order with the amount of 22,523 cases (DKK Padang, 2017). This research aims to determine the effectiveness of the decoction of red betel leaf to decrease blood sugar levels of people with diabetes mellitus. The research design used was Quasy Experimentwith pretests and posttest group. This research conducted in the area of Puskesmas Padang Pasir from April to August 2019. Sampling techniques was purposive sampling with 10 research subjects, 5 people with 100 ml decoction of betel leaf and 5 people are given a 125 ml decoction of betel leaf for three times daily. The results of the average value before and after the intervention of blood sugar levels can be seen a decrease in blood sugar levels of respondents at a dose of 125 ml. In addition, the time of intervention should be more than 7 days for the effectiveness.

The Effectiveness of Ethanol Extract of Red Betel Leaf (Piper crocatum) Againts Mortality of Boophilus microplus Larvae in Vitro

The aims of this study is to know the effectiveness of ethanol extract of red betel leaf (Piper crocatum) against mortality of Boophilus microplus Larvae in Vitro, as well as knowing lethal concentration 50 (LC50) and lethal concentration 90 (LC90). Method that used in the research was completely randomized design. There were five treatments and each treatment was done in five replications. This research used ten Boophilus microplus larvae in each treatment for all replication. The observation and recording of mortality was done at 1, 2, 3, 4, and 5 hours. Boophilus microplus larvae were declared dead if there was no movement. The obtained data was analyzed using ANOVA factorial. The result of this research show that ethanol extract of red betel leaf (Piper crocatum) has effectiveness against mortality of Boophlius microplus larvae in Vitro. In the extract 10% concentration, there is effectiveness that almost the same as Noticks®. The higher concentration of the extract, the higher the effectiveness against mortality lavae.In probit analysis show that LC50 achieved by concentration 5,4% or 2.700mg/50ml and LC90 achieved by concentration 11,3% or 5.650mg/50ml.

The Effectiveness of Red Bread Leaves on the Healing of Perineum Wound

Perineal wound is a tear in the birth canal or due to an incision made in the perineum during delivery of the fetus. Perineal wound treatment can be done with traditional treatments, namely by using herbal medicines, for example making red betel leaf boiled water as washing water or personal hygiene that can be used. used in healing perineal wounds because in boiled water red betel leaf has many properties and contains many active compounds. The purpose of this study was to determine the effectiveness of red betel leaf decoction in healing perineal wounds. This research method is quantitative analytic observational with this type of research design using a quasi experimental method with a pretest posttest one group design. The study was conducted on 10 August to 25 August 2020 with a sample of 30 people using the total sampling technique. Aspects of data measurement using observation sheets with data analysis. The results showed that all respondents (100%) experienced healing of perineal wounds. The results of data analysis obtained Z value = -4460b and p-value = 0.000 with a significance level of p <0.05

The Period of Perineal Wound Healing in Postpartum Mothers Between The Decoction Water Treatments of Bihanong Leaves with Red Betel Leaves

Background: The global prevalence of the second degree of the perineal wound of postpartum mothers is 73.4%. Pharmacological treatments have a cytotoxic effect. Other treatments is non-pharmacological treatments are such as using decoction waters of binahong leaves and red betel leaves. Both have compounds that accelerate epithelialization of wounds. This study aims to analyze the differences of the perineal wound recovery period of postpartum mothers between the decoction water treatments of binahong leaves compared to red betel leaves. Methods: This study is a quasi-experiment post-test only without control group design. There are 32 respondents meeting the inclusion criteria who are taken by accidental sampling. The sample is divided equally into two groups, namely binahong group and the red betel group. Binahong or red betel leaf decoction waters are used for genital washing after urinating at the last rinse at 7 am and 3 pm on 1-3 postpartum days. Perineal wound recovery is assessed by using the REEDA score from the second postpartum day un􀦞l the wound fully recovered. Statistic analysis with chi-square, time series analysis, Mann Whitney and Kruskal Wallis test. Results: The average of perineal wound recovery period of binahong group is 6 days, while the red betel group is 4.69 days. There are significant differences of perineal wound recovery period between the binahong group and the red betel group with a p-value of 0.0001 < α (0.05). Respondents' characteristics are age, BMI, frequency of changing pads, and educational background between binahong and red betel groups have no significant differences. Conclusion: Red betel leaf decoction water is recommended for postpartum mothers suffering perineal wounds. Further research is needed with a larger number of respondents and true experiments are needed to analyze the effect of red betel leaves on perineal pain, platelets, and leukocyte level. Keywords: binahong leaves, red betel leaves, perineal wound's treatment Received: 25 April 2019 Reviewed: 28 May 2019 Revised: 20 June 2019 Accepted: 5 July 2019 DOI: 10.35898/ghmj-33583

The Period of Perineal Wound Healing in Postpartum Mothers Between The Decoction Water Treatments of Bihanong Leaves with Red Betel Leaves

Background: The global prevalence of the second degree of the perineal wound of postpartum mothers is 73.4%. Pharmacological treatments have a cytotoxic effect. Other treatments is non-pharmacological treatments are such as using decoc on waters of binahong leaves and red betel leaves. Both have compounds that accelerate epithelializa on of wounds. This study aims to analyze the differences of the perineal wound recovery period of postpartum mothers between the decoc on water treatments of binahong leaves compared to red betel leaves. Methods: This study is a quasi-experiment post-test only without control group design. There are 32 respon- dents mee ng the inclusion criteria who are taken by accidental sampling. The sample is divided equally into two groups, namely binahong group and the red betel group. Binahong or red betel leaf decoc on waters are used for genital washing a er urina ng at the last rinse at 7 am and 3 pm on 1-3 postpartum days. Perineal wound recovery is assessed by using the REEDA score from the second postpartum day un l the wound fully recovered. Sta s c analysis with chi-square, me series analysis, Mann Whitney and Kruskal Wallis test. Results: The average of perineal wound recovery period of binahong group is 6 days, while the red betel group is 4.69 days. There are significant differences of perineal wound recovery period between the binahong group and the red betel group with a p-value of 0.0001 < α (0.05). Respondents’ characteris cs are age, BMI, frequency of changing pads, and educa onal background between binahong and red betel groups have no significant differ- ences. Conclusion: Red betel leaf decoc on water is recommended for postpartum mothers suffering perineal wounds. Further research is needed with a larger number of respondents and true experiments are needed to analyze the effect of red betel leaves on perineal pain, platelets, and leukocyte level.

Efektivitas Air Rebusan Daun Sirih Merah terhadap Penurunan Kadar Gula Darah pada Pasien Diabetes Melitus di Wilayah Kerja Puskesmas Saling 2018

Diabetes mellitus is a chronic disease that occurs due to the pancreas not being able to produce enough insulin or when the body cannot effectively use the insulin. The purpose of this study was to study the effectiveness of the provision of red betel leaf boiled water on reducing blood sugar levels of Diabetes Mellitus patients in the Saling Puskesmas District of Four Lawang in 2018. The design of this study was an experimental Quasy using The One Group Pretest - Postest Design, technique data collection using primary and secondary data. The population of this study were all Diabetes Mellitus patients in the Saling Community Health Center in July-August 2018, amounting to 16 people, sampling was done by accidental sampling. The results of the study, from 16 patients with diabetes mellitus there are 9 people (56.2%) with blood sugar levels less than 200 mg / dl. Wilcoxon Sign Rank test results obtained value Z = -3.517 with p = 0.000 0.05 means significant. So the two variables have different medians, there are differences in the GDS levels of Diabetes Mellitus patients before and after administration of red betel leaf decoction water. Conclusion, Red betel leaf decoction water is significantly effective in reducing blood sugar levels in patients with diabetes mellitus. It is expected that Puskesmas staff can increase public knowledge by counseling about red betel leaves as a Non-Pharmacological drug for patients with Diabetes Mellitus who do not experience complications. Keywords: Red Betel Leaf Decoction Water, Diabetes Mellitus, Blood Sugar Levels

PENGARUH PEMBERIAN REBUSAN DAUN SIRIH MERAH TERHADAP KEPUTIHAN PATOLOGIS PADA REMAJA PUTRI DI SMA NEGERI 1 MERBAU

Pathological leucorrhoea is a vaginal discharge that occurs due to a bacterial infection, a fungus in which the fluid that comes out a lot and continuously from the vagina and the color of the liquid is not clear or white or yellow or greenish. This study aims to determine the effect of giving red betel leaf decoction to pathological vaginal discharge in young women in Merbau 1 High School. This study used a quasy experimental design, purposive sampling technique, and a sample of 46 people in which 23 were in the intervention group and 23 were in the control group. The sheet pre test and post test is an instrument in this study which contains signs and symptoms of vaginal discharge. The intervention group used red betel leaf boiled water by soaking the female organs for 10-15 minutes, while the control group used ordinary water. The results of the pre-test in the intervention group were 14 students with moderate vaginal discharge and 9 mild vaginal discharge, while 22 post-test students who had recovered vaginal discharge and 1 vaginal discharge were mild. The results of the pre test in the control group were 21 students who had moderate vaginal discharge and as many as 2 people with mild vaginal discharge while the results of the post test, students who had moderate vaginal discharge were as many as 23 people. The increase in the incidence of leucorrhoea from 2 female students who experienced mild vaginal discharge to moderate vaginal discharge in the control group showed that red betel leaf decoction water was able to overcome pathological vaginal discharge compared to normal water. Based on the results of the Wilcoxon test p value = 0.000, which means that there is an effect of giving red betel leaf stew to pathological vaginal discharge in young women in Merbau 1 High School. Researchers suggested that health workers, especially nurses, be able to introduce more about the benefits of red betel leaf decoction to the community, especially women, so that people were more confident in using non-pharmacological treatments.

The Effects of Red Betel (Piper crocatum) Leaf Extract to Decreasing Profile Population of CD4+ and CD8+ Cells in Rheumatoid Arthritis-Induced Mice

Rheumatoid Arthritis (RA) is one of the autoimmune diseases resulted by oxidative stress. Red betel leaves (Piper crocatum) with a high content of flavonoid are expected to function as antioxidant and to reduce inflammation. This research aimed to identify the effects of red betel leaf extract on the amount of the CD4+ and CD8+ cells. 24 male Swiss mice, aged eight 8 weeks, with the weight of 27±3 g. 20 mice induced-RA were made by injecting 0.01 mL Complete Freund’s Adjuvant (CFA) by intraperitoneal; after seven days, they were then boostered by injecting 0.03 mL of Incomplete Freund’s Adjuvant (IFA) into the forelimbs. The animals were divided into six groups: normal, RA (C-), RA+aspirin (C+), RA+red betel leaf extract 100 mg/kg BW (T1), RA+red betel leaf extract 200 mg/kg BW (T2), and RA+leaf extract red betel nut 400 mg/kg BW (T3). The animals were given treatment for 21 days. At the end of the treatment, the mice were sacrificed, and the removed lymphocyte cells were isolated to measure the number of the CD4+ and CD8+ using Flow-cytometry. The results of this research showed that red betel leaf extract was significantly different to decreasing the amount of the CD4+ cells, but it was not significantly different to reducing the amount of CD8+ cells. The effective dosage in this research was 200 mg/kg BW.

Effect of Forms of Pharmaceutical Preparations on Antibacterial Activity of Red Betel Oil (Piper crocatum Ruiz & Pav.)

The Red Sirih Essential Oil (RSEO) which be obtained from red betel leaf (Piper crocatum Ruiz & Pav.) by water and vapour distillation has potential as antibacterial activity. Formulation of an active pharmaceutical ingredient to become a pharmaceutical dosage form can generally modify their activity. The aim of the study is to proof the influence of dosage forms to the activity of RSEO. The study was done by formulating the RSEO to become emulsion, microemulsion and solubilisation dosage forms, and then, their antibacterial activity againt Escherichia coli ATCC 25922 by microdilution method of 1.25% using ELISA reader at 570 nm. The results showed that formulation reduced antibacterial activity. The raw RSEO had a Bacterial Growth Inhibition (BGI) of 66.59%, whereas microemulsion had 49.58%, emulsion had 17.45%, and solubilisation had 0% of BGI, that mean lost its antibacterial activity.

PENGARUH PEMBERIAN REBUSAN DAUN SIRIH MERAH TERHADAP KEPUTIHAN PATOLOGIS PADA REMAJA PUTRI

Pathological leucorrhoea is a vaginal discharge that occurs due to a bacterial infection, a fungus in which the fluid that comes out a lot and continuously from the vagina and the color of the liquid is not clear or white or yellow or greenish. This study aims to determine the effect of giving red betel leaf decoction to pathological vaginal discharge in young women in Merbau 1 High School. This study used a quasy experimental design, purposive sampling technique, and a sample of 46 people in which 23 were in the intervention group and 23 were in the control group. The sheet pre test and post test is an instrument in this study which contains signs and symptoms of vaginal discharge. The increase in the incidence of leucorrhoea from 2 female students who experienced mild vaginal discharge to moderate vaginal discharge in the control group showed that red betel leaf decoction water was able to overcome pathological vaginal discharge compared to normal water. Based on the results of the Wilcoxon test means that there is an effect of giving red betel leaf stew to pathological vaginal discharge in young women in Merbau 1 High School. Researchers suggested that health workers, especially nurses, be able to introduce more about the benefits of red betel leaf decoction to the community, especially women, so that people were more confident in using non-pharmacological treatments.

Antibacterial activity of fractions (chloroform, ethyl acetate and methanol) red betel leaf ethanol extract (Piper crocatum) against Streptococcus pyogenes bacteria

Antibacterial activity of fractions (chloroform, ethyl acetate and methanol) red betel leaf ethanol extract (Piper crocatum) against Streptococcus pyogenes bacteria

Aktivitas Antibakteri Minyak Sirih Merah (Piper Crocatum Ruiz & Pav.) Dalam Bentuk Sediaan Emulsi dan Mikroemulsi

Minyak Sirih Merah (MSM) diketahui mempunyai aktivitas sebagai antibakteri dan dapat dibuat dengan cara distilasi air dan uap dari daun sirih merah (Piper crocatum Ruiz & Pav.). Tujuan dari studi ini adalah untuk membandingkan aktivitas antibakteri dari MSM apabila diformulasi dalam bentuk sediaan yang berbeda (yaitu emulsi dan mikroemulsi). MSM dalam bentuk emulsi dibuat dengan cara mencampur 0,125 g MSM, 0,875 g paraffin cair, 0,125 g tween-80, 0,375 g span-80 (HLB 7), dan 3,500 g air. MSM dalam bentuk mikroemulsi dibuat dengan mencampur 0,125 g MSM, 0,125 g paraffin cair, 0,625 g tween-80, 0,625 g PEG-400, dan 3,500 g air. Aktivitas antibakteri terhadap Escherichia coli ATCC 25922 dilakukan dengan menggunakan metoda mikrodilusi dengan 1% w/w konsentrasi menggunakan reader ELISA pada 570 nm. Data kemudian dianalisis untuk membandingkan persentase penghambatan pertumbuhan mikroba. Hasil penelitian menunjukkan bahwa hambatan bakteri dari MSM dalam bentuk emulsi adalah 17,45±5,9% sedangkan dalam bentuk mikroemulsi 49,58±3,27%. Kesimpulannya adalah aktivitas antibakteri MSM dalam bentuk mikroemulsi lebih baik dari bentuk emulsi.

The effectiveness of red betel leaf (Piper crocatum) extract against periodontal pathogens

Introduction: Red betel (Piper crocatum) has long been known as one of herbs that has antibacterial properties as it contains several useful compounds such as essential oils, flavonoid, alkaloids and tannins.  The objective of this study is to learn more about the effectiveness of red betel leaf extract based on in vitro test against periodontal pathogen bacteria such as Aggregatibacter actinomycetemcommitans (Aa) and Porphyromonas gingivalis (Pg).Method: This study was conducted using diffusion method that has Kirby-Bauer test sensitivity. The experiment was performed eight times on four treatments consisting of 10% DMSO as negative control and 2.5%, 5% and 10% of red betel leaf extract. The sterile paper disc was soaked into sterile water and red betel leaf extract at each concentration before evaporation inside the incubator.  After that, the dry paper disc was placed in the Nutrient Agar (NA) that had previously been inoculated and incubated for 24 hours at 37° Celsius. ANOVA test was used to compare inhibition zone in four group intervention.Result:  The result of the study shows that the 2.5%, 5% and 10% concentration of red betel leaf extract respectively could inhibit the growth of bacteria Aa and Pg. The result of the study also confirms that the 10% red betel leaf extract concentration created the largest inhibition zone (10.5786 mm for bacteria Aa and 10.7638 mm for bacteria Pg).Conclusion: Red betel leaf extract, particularly at 10% concentration is significantly effective in inhibiting the growth of Aa and Pg bacteria.Â

PERBEDAAN DAYA HAMBAT ANTIBAKTERI ANTARA EKSTRAK DAUN SIRIH MERAH (Piper crocatum) DAN EKSTRAK DAUN SIRIH HIJAU (Piper betle L.) TERHADAP BAKTERI Streptococcus mutans

Background: Red Betel ( Piper crocatum ) and green betel ( Piper betle L.) is believed to have efficacy preventing dental caries disease caused by the bacterium Streptococcus mutans . Purpose: Research purpose is to review describes different inhibitory extract of red betel leaf and leaf green betel Against bacteria Streptococcus mutans . Materials and Methods: Operations Research is an experimental by design post test only group design. Sample Research : is a pure culture of Streptococcus mutans bacteria in the laboratory of Microbiology, Faculty of Pharmacy, University Muslim Indonesia. Analitical statistics to be done using One Way ANOVA. Furthermore, the Post Hoc multiple comparison test for review red betel Differences And green betel inhibit bacteria. Results: Based on the statistical test showed that the zone of inhibition power of 4 Different the concentration (0.1%, 1%, 10%, 100%) As well as having a significant difference between the two TYPE betel leaf against bacteria Streptococcus mutans . Conclusion: The findings of the data analysis can be concluded that the green betel leaf showed inhibitory better than red betel leaf against bacteria Streptococcus mutans .

The comparison of two neolignans isolated from red betel leaf and its extract against macrophage phagocytic activity, the level of AST, and histopathological features of the liver in mice

A single active compound isolated from a plant extract may show a lower, equal, or greater activity compared to its extract. The two neolignans (Pc-1 and Pc-2) has been isolated from red betel (Piper crocatum Ruiz & Pav.) leaf. The aim of this study was to compare the phagocytic activity of macrophage and histopathological feature in mice treated with Pc-1, Pc-2 and its Pc-extract. Listeria monocytogenes was used to induce Balb/c mice immune response. Peroral administration of Pc-1 and Pc-2 and red betel leaf methanolic Pc-extract were carried out. The phagocytic effect was determined by macrophage phagocytosis and nitric oxide (NO) assays. The morphological feature of liver and kidney were observed using light microscope. The level of apartate transaminase (AST serum) and alanine transaminase (ALT serum) were also measured from the blood serum before and after L. monocytogenes infection. The results shows that the macrophage phagocytic activity of given 450 mg/kg body weight Pc-extract was equal to 5 mg/kg body weight of Pc-1 and Pc-2. The activity pattern of percentage phagocytosis, index phagocytosis, and efficiency phagocytosis of Pc-extract, Pc-1, and Pc-2 were similar, as well as the NO production. A certain dose of Pc-extract and both isolated compound reduced the level of AST while there were no effect on ALT level. There were no histopathological features differences in the liver after treated with Pc-extract and Pc-1. However, Pc-2 treatment caused hydropic degeneration on liver. Therefore it can be concluded that there were equal activity between Pc-extract and the isolated compound in certain dose. The Pc-extract and isolated compound reduced the level of AST while there were no effect on ALT. The only Pc-2 affected the histopathological features of the liver.