PT. X is a textile industrial company founded in 1975, starting commercial production in 1976 with a cotton spinning factory in Purwakarta. However, even though it has been established for a long time in Indonesia, there are still defects that occur in the results of production activities at Spinning Division 6, namely the division that produces 100% cotton yarn. These defective products will reduce the company's reputation where consumer loyalty to the company is at stake, some even do not want to cooperate and negotiate with companies that have a poor reputation. The aim of this research is to find out the factors that cause defective products to be able to evaluate and improve the scope of production activities so as to minimize the occurrence of defects in production results again and improve zero defect quality control. The analysis method in this research can be carried out using the 5 Wyhs analysis through the Root cause analysis approach which is used to find out the root of a problem that causes defects in production results. There are errors that occur along with the factors that are carried out. One of them is that stains on the rolls occur on MCR machines, this is caused by a lack of supervision and checking of the pallet layers to be used. Rolls going off track occur on the winding machine because the glider is damaged so that during the thread cutting process the glider stops spinning for a long time resulting in the thread being thrown to the side so that when the operator starts the machine again, the rolls come out of the cone track. Tangles occur due to the suction mouth touching the surface of the reel, resulting in the thread being stuck when the reel rotates.