Jet-in-crossflow (JICF) is a potential and reliable technique that can promote flame acceleration and deflagration-to-detonation transition (DDT). Most of the previous researches utilized combustible mixture or oxygen as the medium of JICF, therefore the detonation enhancement effect may be partly attributed to the chemical properties of the jet, and the purely turbulent effect induced by JICF on DDT has not been fully understood. In our previous work, the non-reactive gas jet affected flame propagation indirectly by separating the injection and ignition in the control sequence. A competing mechanism between the positive combustion-enhancement effect caused by jet-induced turbulence and the negative non-reactive gas-inhibition effect on combustion has not been found, the non-reactive gas jet with high pressure and short duration time was also verified to be beneficial for precursor shock wave formation and flame acceleration. However, no detonation was observed in the work. In this paper, the non-reactive jet interacts with the flame propagation directly by overlapping the working time of the jet and ignitor partly, and the effect of jet parameters including injection pressure and time delay on DDT is investigated systematically. The results show that the flame affected by the non-reactive gas jet with a high-pressure ratio and a short time delay can transit to detonation in a short run-up distance successfully. The schlieren images of the flame evolution process illustrate that the flame interacted by the jet experiences four states: layered flame, wrinkled flame, cellular flamelets, and turbulent flame. Kelvin–Helmholtz (K-H) instability and Rayleigh-Taylor (R-T) instability dominate in the flame distortion. The characteristic timescales of the flow and reaction are computed in the four flame states, turning out that as the flame evolving, the turbulent intensity of the flame is enhanced. Damköhler number (Da) on the flame front eventually converges to [1,2] demonstrates the characteristic timescales of the turbulence and reaction are on the same order, and the turbulence dominates the flame evolution.