The quality of the resulting bread was evaluated by conventional methods including the determination of the content of protein, dietary fiber, fat and its fatty acid composition. To achieve this goal, we used approaches that combined both traditional and non-traditional food raw materials, namely gluten-free flour of chia seeds (a source of PUFA and dietary fiber), lupin flour (a source of protein), as well as enzyme transesterificated milk fat substitute containing ω-3 PUFAs. A protein-polysaccharide mixture was used as a thickener and structure-forming agent. The developed formulations allowed to obtain finished products characterized by the content of ω-3 fatty acids - 0.4-0.6 g/100 g, while maintaining a balance between essential ω-6 and ω-3 fatty acids at the level (2.5-3.5):1, recommended for therapeutic nutrition, dietary fiber - 11.1-12.4 g/100 g, and protein content - 5.2-6.7 g/100 g, while maintaining good organoleptic properties. The achieved level of enrichment in accordance with the current legislation allows labeling the resulting products as «high in ω-3 fatty acids and dietary fibers». According to the available data on the biological effect of the components of the developed product, it can affect lipid metabolism, contributing to the reduction of atherogenic lipids in the blood. The implementation of the results of this work in the industry will expand the range of gluten-free products through the production of specialized bakery products with the strengthening of their dietary therapeutic and preventive orientation due to the high content of ω-3 fatty PUFAs and dietary fibers.