Ratiometric Fluorescent Probe Research Articles

Dual excitation channel ratiometric fluorescent probes for visual and fluorescent detection of anthrax spore biomarker and tetracycline hydrochloride

Long-term and excessive use of tetracycline hydrochloride (TC) can lead to its accumulation in the environment, which can cause water contamination, bacterial resistance, and food safety problems. 2,6-Pyridine dicarboxylic acid (DPA) is a major biomarker of Bacillus anthracis spores, and its rapid and sensitive detection is of great significance for disease prevention and counter-terrorism. A bifunctional ratiometric fluorescent nanoprobe has been fabricated to detect DPA and TC. 3,5-dicarboxyphenylboronic acid (BOP) was intercalated into layered europium hydroxide (LEuH) by the ion-exchange method and exfoliated into nanosheets as a fluorescent nanoprobe (PNP). DPA and TC could significantly enhance the red fluorescence of Eu3+ through the antenna effect under different excitation wavelengths, while the fluorescence of BOP can be used as a reference based on the constant emission intensity, realizing ratiometric detection. A low limit of detection (LOD) for the target (DPA: 9.7 nM, TC: 21.9 nM) can be achieved. In addition, visual detection of DPA and TC was realized using color recognition software based on the obvious color changes. This is the first ratiometric fluorescent nanoprobe based on layered rare-earth hydroxide (LRH) for the detection of DPA and TC simultaneously, which opens new ideas in the design of multifunctional probes.

An effective turn-on/off rodamine-encapsulated UiO-67-NH2 fluorescent probe for simultaneous As5+/Fe3+ detection

The development of metal ion detection probes with high sensitivity and selectivity is of utmost importance for the promotion of public health and environmental sustainability. In this work, a series of ratiometric fluorescent probes (RhB@UiO-67-NH2) were successfully prepared using a one-pot method for the detection of metal ions, particularly As5+ and Fe3+. Notably, the ratiometric fluorescent probe RhB@UiO-67-NH2 (1:4) demonstrates dual functionality as a Fe3+ turn-off probe and an As5+ turn-on probe. The limits of detection (LODs) for As5+ and Fe3+ using RhB@UiO-67-NH2 (1:4) were determined to be 0.521 μM (39.03 ppb) and 0.107 μM (5.97 ppb), respectively, which were the lowest records of reported LMOFs so far. The fluorescence quenching of Fe3+ can be attributed to various mechanisms such as fluorescence resonance energy transfer (FRET), photoinduced electron transfer (PET), and competitive absorption (CA). Additionally, the fluorescence enhancement of As5+ is primarily due to absorbance-caused enhancement (ACE) and PET. Moreover, the composite material RhB@UiO-67-NH2 (1:4) exhibited excellent anti-interference capability and reproducibility for detecting Fe3+ and As5+. The removal efficiency of As5+ by RhB@UiO-67-NH2 (1:4) exceeded 50.9 % when the initial concentration of As5+ was below 20 mg/L. This work presents a valuable reference for future investigations and utilization of As5+ and Fe3+ sensing.

Real time precisely tracing the fluctuations of mitochondrial SO2 in cells during ferroptosis and tissues using a mitochondrial-immobilized ratiometric fluorescent probe

Mitochondrial sulfur dioxide (SO2) plays important roles in physiological and pathological activities. Unfortunately, it is lack of a reliable tool to precisely visualize the mitochondrial SO2 and elaborate its complicated functions in various cytoactivities. Here we report a mitochondrial-immobilized fluorescent probe PM-Cl consisting of coumarin and benzyl chloride modified benzothiazole, which enables selective visualization of mitochondrial SO2via chemical immobilization. The spectral results demonstrated that probe PM-Cl could respond to SO2 with high selectivity and sensitivity. Co-localization and the fluorescence of cytolysis extraction verified the excellent mitochondrial targeting and anchoring abilities. Due to the chemical immobilization, probe PM-Cl could firmly retain into mitochondria after stimulation of carbonyl cyanide m-chlorophenyl hydrazone (CCCP) and H2O2. Significantly, a series of fluorescence images are indicative of capability for detecting the fluctuations of SO2 in mitochondria during ferroptosis. Furthermore, PM-Cl also could visualize SO2 in myocardium and muscle tissues after the stimulation of CCCP. Taken together, probe PM-Cl is a very potential molecular tool for precisely detecting mitochondrial SO2 to explore its complex functions in physiological and pathological activities.

Construction of a multifunctional paper-based fluorescent material for the detection and adsorption of formaldehyde

Indoor air pollution is one of the significant inducing factors of some diseases, such as leukemia, tumors, respiratory disease, and kidney failure. Among these contaminants, formaldehyde has a long release span and is a great threat to our health. However, methods for formaldehyde elimination and detection are limited. Herein, a new paper-based multifunctional fluorescent material was developed for the simultaneous high-efficiency removal and detection of formaldehyde. The paper-based material was fabricated using polyethyleneimine (PEI) and a highly sensitive ratiometric fluorescent probe (FP). The paper-based material can detect formaldehyde with a significant change in fluorescence: the fluorescence at 450 nm decreased by over 75 %, and the fluorescence of FP in the ratiometric channel increased by over 100-fold. Moreover, the formaldehyde elimination rate is up to 99 %. By combining the porous network structures of paper, the abundant amino groups in PEI, and the highly sensitive fluorescent response of FP, the multifunctional fluorescent paper material can be effectively utilized for formaldehyde removal and detection.

A ratiometric fluorescent probe for selective detection of hypochlorite (ClO−) and gallium (III) (Ga3+) ions in environmental and food samples

Hypochlorite (ClO−) and gallium (Ⅲ) ions (Ga3+) have extensive applications in various human industries and daily activities. However, their inherent toxicity poses significant risks to environmental preservation and human well-being. Hence, the development of reliable and handy detection tools for ClO− and Ga3+ in the environment and food is crucial. In this study, a ratiometric fluorescent probe was prepared based on benzothiazolaldehyde and pyridine-2-carboxylic acid hydrazide, which exhibited exceptional performance characteristics for the selective detection of ClO− and Ga3+. These features include high specificity, low detection limits (0.28 μM for ClO−, 0.13 μM for Ga3+), mild pH conditions (pH 4–11 for ClO−, pH 6–11 for Ga3+), fast response time (within 30 s), as well as versatile applicability across different matrices such as water, soil, food, and plant samples. Additionally, this probe can be used with a smartphone color recognition app. The probe offers a convenient and effective tool for the detection of ClO− and Ga3+, demonstrating its potential application value in environmental monitoring and food safety.

A ratiometric fluorescent probe for the determination of quinolone antibiotics in milk based on N and S co-doped carbon quantum dots

Quinolone antibiotics are widely used to prevent and treat diseases caused by bacterial infection. However, overuse of antibiotics may lead to their residue in the environment and food, posing potential threats to human health. In this study, a ratiometric fluorescent probe based on nitrogen and sulfur co-doped carbon quantum dots (N, S-CQDs) was synthesized, which can realize the detection of moxifloxacin (MXF), gatifloxacin (GAT) and ofloxacin (OFX) in milk. After addition of these antibiotics, the fluorescence at 440 nm originating from N, S-CQDs was significantly quenched due to the fluorescence resonance energy transfer (FRET), while the fluorescence at 525 nm (MXF), 500 nm (GAT) and 515 nm (OFX) were enhanced due to aggregation induced emission (AIE). The fluorescence changes were completed within 30 s and stabilized for at least 14 days. Good linear relationships between the fluorescence intensity ratios (F525/F440 for MXF, F500/F440 for GAT and F515/F440 for OFX) and the antibiotic concentrations ranging from 0.1 to 100 mg L−1 were obtained, with R2 above 0.99. The limits of quantitation were in the range of 1.67–6.20 μg L−1, and MXF, GAT and OFX can be well identified by linear discriminant analysis. The probe enabled quantitative analysis of MXF, GAT and OFX in milk samples, with recoveries ranging from 82% to 107%. A test strip based on the ratiometric fluorescence probe was successfully applied for the detection of MXF and GAT in milk in combination with a smartphone. This method exerted high specificity and sensitivity, which has the potential to achieve in-site detection of MXF, GAT and OFX in milk.

Ratiometric fluorescent probe with AIE characteristics for hypochlorite detection and biological imaging

It is very important and highly valuable to detect ClO− in samples and living cells with accuracy and speed. In this work, a novel fluorescent probe NA was prepared from 4-bromo-1,8-naphthalic anhydride by acylation reaction and Suzuki coupling reaction and used for the detection of ClO−. Thiomethyl serves as the recognition group for probe NA, while naphthalimide serves as fluorescent chromophore. The probe exhibited an extremely pronounced blue shift from yellow to blue fluorescence within 1 min after the addition of hypochlorite (ClO−). The probe demonstrates high sensitivity to ClO− with a limit of detection (LOD) of 1.22 µM. Also, probe NA demonstrates excellent selectivity and immunity to interference. Additionally, simple fluorescent test strips containing probe NA were prepared in this study, enabling rapid detection of ClO− in water samples. And NA had been effectively used to image endogenous and exogenous ClO−fluorescence in living cells. The results suggest that probe NA has significant potential for portable detection and biological applications.

Facile construction of a bifunctional Eu-MOF for selective fluorescence sensing and visible light photocatalysis of tetracycline

Contamination detection and removal are important steps in environmental monitoring and cleanup. In this study, europium metal-organic framework structures (Eu-MOF) were designed and prepared that can act as fluorescent probes and photocatalysts for the detection and removal of tetracycline (TC) antibiotics. Eu-MOF was generated by a one-step solvothermal method using europium ion as the metal ion and terephthalic acid as the organic ligand source. The red fluorescence of Eu-MOF is selectively enhanced by TC due to the antenna effect. Eu-MOF, which has a linear range of 0.5–60 μM, can be utilized as a fluorescent probe for tetracycline detection based on this phenomena. In particular, Eu-MOF was found to be able to effectively photocatalyze the degradation of TC, with tetracycline removal rates of about 70 % and 55 % after 180 min of irradiation under ultraviolet light (UV) light and simulated visible light, respectively. The photocatalytic ability of Eu-MOF originated from the generation of hydroxyl radicals (•OH), superoxide radicals (•O2−) and electron holes (h+) under light irradiation. This study innovatively proposed a bifunctional Eu-MOF for TC fluorescence detection and photocatalytic degradation, which provides a novel method for the quick and easy identification and elimination of pharmaceutical contaminants. It can also provide some reference value for the construction of ratiometric fluorescent probes and heterojunction photocatalysts and their application in the treatment of water pollution.

Development of a Pyrone-Fused Tricyclic Scaffold-based Ratiometric Fluorescent Probe for Al3+ Detection.

Aluminum (Al3+) is environmentally abundant and can harm living organisms in various ways, such as by inhibiting root growth, damaging faunal nervous systems, and promoting tumor cell proliferation. However, the dynamics of Al3+ in living organisms are largely unknown; thus, detecting Al3+ in the environment and organisms is crucial. Fluorescent probes are useful tools for the selective detection of metal ions. In particular, ratiometric fluorescent probes exhibit a detection response at two different maximum fluorescence emission wavelengths; which is advantageous for avoiding the influence of background fluorescence. A novel pyrone-fused tricyclic scaffold-based ratiometric fluorescent probe for detecting Al3+, ethyl 11-imino-1-oxo-3-phenyl-1H,11H-pyrano[4,3-b] quinolizine-5-carboxylate (PQ), was developed in this study. The PQ fluorescence blue shifted from 505 to 457nm upon the addition of Al3+. The blue shift was accompanied by a change in the fluorescence color of the PQ solution from green to blue. Fluorescence titration experiments demonstrated that the fluorescence intensity ratio at the two peaks of interest (457/505nm) increased in a concentration-dependent manner upon the addition of Al3+. Moreover, this study demonstrated that a PQ-soaked paper displays a visible color change under ultraviolet light upon exposure to Al3+. The above results suggest that PQ is an effective ratiometric probe for the detection of Al3+ in the environment. Future studies will be conducted to introduce various substituents and develop fluorescent probes by leveraging the fluorescence property of a pyrone-fused tricyclic scaffolds.

Enhancing Kinase Activity Detection with a Programmable Lanthanide Metal-Organic Framework via ATP-to-ADP Conversion.

Precise modulation of host-guest interactions between programmable Ln-MOFs (lanthanide metal-organic frameworks) and phosphate analytes holds immense promise for enabling novel functionalities in biosensing. However, the intricate relationship between these functionalities and structures remains largely elusive. Understanding this correlation is crucial for advancing the rational design of fluorescent biosensor technology. Presently, there exists a large research gap concerning the utilization of Ln-MOFsto monitor the conversion of ATP to ADP, which poses a limitation for kinase detection. In this work, we delve into the potential of Ln-MOFs to amplify the fluorescence response during the kinase-mediated ATP-to-ADP conversion. Six Eu-MOFs were synthesized and Eu-TPTC ([1,1':4',1″]-terphenyl-3,3'',5,5''-tetracarboxylic acid) was selected as a ratiometric fluorescent probe, which is most suitable for high-precision detection of creatine kinase activity through the differential response from ATP to ADP. The molecular -level mechanism was confirmed by density functional theory. Furthermore, a simple paper chip-based platform was constructed to realize the fast (20 min) and sensitive (limit of detection is 0.34 U/L) creatine kinase activity detection in biological samples. Ln-MOF-phosphate interactions offer promising avenues for kinase activity assays and hold the potential for precise customization of analytical chemistry.

A coumarin-naphthalimide-based ratiometric fluorescent probe for nitroxyl (HNO) based on an ICT-FRET mechanism

Nitroxyl (HNO) is an important reactive nitrogen that is associated with various states in physiology and pathology and plays a unique function in living systems. So, it is important to exploit fluorescent probes with high sensitivity and selectivity for sensing HNO. In this paper, a novel ratiometric fluorescent probe for HNO was developed utilizing intramolecular charge transfer (ICT) and fluorescence resonance energy transfer (FRET) mechanisms. The probe selected coumarin as energy donor, naphthalimide as energy receptor and 2-(diphenylphosphino)benzoate as the sensing site for detecting HNO. When HNO was not present, the 2-(diphenylphosphino)benzoate unit of the probe restricted electron transfer and the ICT process could not occur, leading to the inhibition of FRET process as well. Thus, in the absence of HNO the probe displayed the intrinsic blue fluorescence of coumarin. When HNO was added, the HNO reacted with the 2-(diphenylphosphino)benzoate unit of the probe to yield a hydroxyl group which resulting in the opening of ICT process and the occurring of FRET process. Thus, after providing HNO the probe displayed yellow fluorescence. In addition, the probe showed good linearity in the ratio of fluorescence intensity at 545 nm and 472 nm (I545 nm/I472 nm) with a concentration of HNO (0.1–20 μM). The probe processed a detection limit of 0.014 μM and a response time of 4 min. The probe also specifically identified HNO over a wide pH scope (pH = 4.00–10.00), including physiological conditions. Cellular experiments had shown that this fluorescent probe was virtually non-cytotoxic and could be applied for ratiometric sensing of HNO in A549 cells.

Ratiometric detection and monitoring of cyanide in biological, environmental and food samples by a novel triphenylamine-xhantane based fluorescent probe

BackgroundAs cyanide (CN−) is a significant hazard to the environment and human health, it is essential to monitor cyanide levels in water and food samples. Moreover, real-time visualization of CN−could provide an additional understanding of its critical physiological and toxicological roles in living cells. The fluorescence approach based on small organic probes is an effective way for the detection of CN−. In this approach, a triphenylamine-xhantane conjugate was applied to detect in many samples such as sewage water, soil, sprouted potato, apricot seed, and living cells. ResultsWe report a new ratiometric near-infrared fluorescent probe based on a triphenylamine-xhantane derivative for CN−sensing in many samples. The probe displays high selectivity for only CN− ions among a series of analytes. The addition of cyanide to the dicyanovinyl moiety of the probe disrupts π-conjugation followed by the interruption of internal charge transfer. Consequently, the emission peak of the probe shifts hypsochromically from 655 to 495 nm. There is a linear correlation between the emission intensity (I495) and cyanide level, with a detection limit of 0.036 μM. The probe has many advantages over many probes, such as NIR fluorescence, ratiometric response, low cytotoxicity (85.0 % cell viability up to 50.0 μM of the probe), good membrane permeability, fast response time (4.0 min), high selectivity, good photostability, and anti-interference capability. SignificanceAlthough various probes have been reported in the literature, the use of triphenylamine-xhantane unit as CN− probe has yet to be explored. The probe can detect trace levels of cyanide in many samples such as sewage water, soil, sprouted potatoes, and apricot seeds. Furthermore, it is successfully utilized for the ratiometric fluorescent bioimaging of cyanide in living cells.

Lateral flow assay with dual-emission nitrogen-doped carbon dots for ratiometric fluorescent determination of nitrite in food

Nitrite ion (NO2–), renowned for its potent antiseptic properties and color-preserving abilities, potentially leads to carcinogenesis and hypertension if consumed excessively. Herein, a ratiometric fluorescent probe exhibits specific response to NO2– using nitrogen-doped carbon dots (N-CDs) with a low detection limit (LOD) as low as 52 nM and a low quantification limit (LOQ) of 173 nM. Initially, the emission peaks at 495 nm and 415 nm decrease as result of the internal filtration effect (IFE). With further increasing NO2– concentration, the reaction between NO2– and N-CDs forms an azoic compound (N-CDs@NO2–), which promotes the instant quenching of the emission peak at 415 nm through fluorescence resonance energy transfer (FRET). Moreover, portable N-CDs/LFASs devices with smartphone are successfully applied to visually identify NO2– with the exceptional LOD, achieving excellent recoveries and high precision in real sample. Therefore, this newly N-CDs/LFASs offers a reliable means for the NO2– detection, showcasing potential applications.

Visual fluorescent detection of enrofloxacin based on hollow EH-SiO2@ covalent organic framework nanospheres

Covalent organic frameworks (COFs) are porous crystalline polymers. Due to aggregation caused quenching (ACQ) effect caused by the strong π-π interactions in stacked layers, weak fluorescence or no luminescence is caused. Here, a series of core–shell structure of SiO2@COFTFP-AMPD (2,4,6-triformylphloroglucinol: TFP, 2,2′-azobis(2-methylpropionamidine) dihydrochloride: AMPD) with different morphologies was prepared by changing temperature and time using –NH2-SiO2 nanospheres (NPs) as templates. Without the need to be prepared by adding HF or strong alkali, the hollow spherical EH-SiO2@COFTFP-AMPD not only inhibited ACQ of COFTFP-AMPD, but also achieved dual emission at 438 and 550 nm. The structure served as ratiometric fluorescent probe for visual detection of Enrofloxacin (ENR) with a detection range of 2.41 nM-0.48 μM and a detection limit as low as 0.80 nM. The concentration of ENR in unknown samples can be detected not only by stripe and gel. Moreover, with the RGB numerical analysis function of smartphone App, real-time visual detection of ENR can be realized, which possesses potential in food health and safety.

A novel biomass carbon dots ratiometric fluorescence probe coupled with molecularly imprinted polymer for selective detection of aflatoxin B1

The accumulation of Aflatoxin B1 (AFB1) in food and crops can pose significant hazards to human health. Consequently, there has been extensive attention paid to the development of a rapid and reliable method for detecting AFB1 in food. In this context, a ratiometric fluorescence probe (DE-MIPs) was prepared using dual-emission fluorescent carbon dots (CDs) coupled with the molecular imprinting technique for visual detection of AFB1. The dual-emission CDs consisted of red fluorescence biomass CDs (R-CDs) wrapped by molecularly imprinted cavities (R-CDs@MIPs), which served as a response signal, and pure blue emission biomass CDs (B-CDs) as a reference signal. Due to the specific quenching effect of AFB1 on red fluorescence while leaving blue fluorescence unaffected, there was a gradual decrease in the fluorescence intensity ratio (I670/I437) and a distinct shift in fluorescence color from red to blue as the concentration of AFB1 increased. The ratiometric probe demonstrated excellent linearity in detecting AFB1 within the concentration range of 0.1–200 ng/mL, with a detection limit (LOD) of 0.05 ng/mL. Furthermore, the practicality of DE-MIPs was validated by successfully detecting AFB1 in vegetable oil, with desirable recoveries from 89.0% to 107.4%. Furthermore, the integration of a smartphone as colorimetric reader enabled rapid AFB1 detection by capturing changes in fluorescence color. Overall, the development of this ratiometric probe offers a novel perspective for the facile and efficient identification of AFB1 in complex food samples.

A Ratiometric Fast-Response Fluorescent Probe Based on Dicyanoisophorone for Monitoring HClO in Paper Test Strips and Living Mice.

A new dicyanoisophorone-based ratiometric fluorescent probe NOSA was synthesized and characterized. It showed a fast fluorescence response to HClO with the emission color change from dark green to bright red. NMR, IR, and HRMS suggested that the detection of NOSA to HClO may originate from the hydroxyl deprotection reaction by HClO on the molecule NOSA, which caused a red-shift of fluorescence. The probe NOSA displayed high selectivity and excellent anti-interference performance with a limit of detection at 3.835 × 10-7M. The convenient paper test strips were successfully obtained and applied to the detection of HClO based on fluorescence color change with the varied NaClO concentration. Moreover, spiked recovery experiments in real water samples indicated that the probe NSOA could quantitatively detect HClO, and the fluorescence bio-imagings in vivo were carried out, and HClO detection in biosystems using NOSA was realized.

Ratiometric fluorescent determination of sulfadimethoxine in foods based on a dual-emission metal-organic framework.

Ratiometric fluorescence detection is endowed with higher accuracy than single fluorescence signal assay. In this work, we construct a ratiometric fluorescence probe for the facile quantification of sulfadimethoxine (SDM) in foods. By wrapping N-doped carbon dots (N-CDs) and gold nanoclusters (AuNCs) into zeolitic imidazolate framework-8 (ZIF-8), the nanocomposite of N-CDs/AuNCs@ZIF-8 is facilely prepared and emits two fluorescence including 475nm from N-CDs and 650nm from AuNCs. Since bovine serum albumin (BSA) is the stabilizer of AuNCs, SDM can form a complex with BSA, resulting in the fluorescence quenching of AuNCs at 650nm by a static quenching mechanism. In contrast, SDM has a rare influence on the fluorescence of N-CDs (475nm). As a result, the use of the probe of N-CDs/AuNCs@ZIF-8 for SDM detection enables simultaneous measurement of response signal and reference signal. Under the optimal condition, the SDM assay based on the probe has a good linear relationship within 10 to 2 × 106ng/mL and the limit of detection (LOD) is low to 1.064ng/mL. In addition, the fluorescent probe shows good reliability for the detection of SDM in practical food samples.

A Ratiometric Benzimidazole-Based Fluorescent Probe for The Recognition of Phosgene in Solution and Gaseous Phases.

Considering the high toxicity and widespread application of phosgene, there is an urgent need to develop a simple and sensitive method for detecting phosgene. In this work, we designed and synthesized a novel ratiometric fluorescent probe 1 containing fluorophores of benzimidazole and benzothiazole. Probe 1 showed excellent sensitivity (< 30s) and selectivity (LOD = 3.82 nM) for phosgene and significant ratiometric fluorescence changes. In addition, 1-loaded polystyrene membrane test strips were used to conveniently and efficiently detect phosgene gas (0.5 ppm) via the naked eye and the RGB APP of the smartphone, indicating that this probe has great potential for phosgene detection in the gaseous phase.

Ratiometric fluorescent platform for on-site monitoring of sodium pyrosulfite in preserved fruits

The rapid detection of pyrosulfites in food chemistry is crucial to food safety and health. Here, a coumarin-type ratiometric fluorescent probe was developed based on the Michael addition reaction to detect sodium pyrosulfite (Na2S2O5). The probe exhibited high selectivity and fast response (t1/2 = 6 s) to Na2S2O5 and a low detection limit (26 nM). Because of its excellent ratiometric response performance, the probe was successfully applied to measure the amount of Na2S2O5 in preserved fruits. Colour information analysis and formula calculations were performed to quickly determine the sodium pyrosulfite amount in an actual sample by using a smartphone. Therefore, the intelligent strategy of combining the sensing process and smartphone provides a convenient and efficient method for the fast monitoring of sodium metabisulfite in actual food.

A novel fluorescent probe with tailored properties for ozone: Applications in on-site environmental monitoring and real-time bioimaging in Parkinson’s disease models

Ozone (O3) is not only a widespread environmental contaminant at the troposphere or ground level, but also a significant reactive oxygen species (ROS) in vivo that participates in numerous physiological processes. Overexposure of O3 can lead to worsening of neurological diseases, including Parkinson’s disease (PD). Therefore, it is highly desirable to track O3 both in the environment and in vivo. In this work, we rationally designed and synthesized a novel coumarin-based ratiometric and mitochondria-targeting fluorescent probe, namely DPC-O3, for the recognition of O3 with high sensitivity and selectivity. In the environmental system, DPC-O3-doped fluorescent test strips were successfully prepared that allowed on-site detection of O3 in air samples by portable smartphone platform. In the biological systems, probe DPC-O3 can firstly accumulate in mitochondria and then migrate to lipid droplets after treatment of O3. Tracking of O3 was successfully achieved in the models of cellular inflammation and mitochondrial dysfunction. In addition, DPC-O3 was also successfully used to monitor O3 levels in the PD zebrafish model, firstly demonstrating a positive correlation between the fluctuation of endogenous O3 and PD. Hopefully, probe DPC-O3 could be a beneficial tool that may further implicate the potential for further exploration on O3-releated environmental and pathological processes.