Method For Rapid Mapping Research Articles

The reliability and validity of rapid transcranial magnetic stimulation mapping for muscles under active contraction

Rapid mapping is a transcranial magnetic stimulation (TMS) mapping method which can significantly reduce data collection time compared to traditional approaches. However, its validity and reliability has only been established for upper-limb muscles during resting-state activity. Here, we determined the validity and reliability of rapid mapping for non-upper limb muscles that require active contraction during TMS: the masseter and quadriceps muscles. Eleven healthy participants attended two sessions, spaced two hours apart, each involving rapid and ‘traditional’ mapping of the masseter muscle and three quadriceps muscles (rectus femoris, vastus medialis, vastus lateralis). Map parameters included map volume, map area and centre of gravity (CoG) in the medial-lateral and anterior-posterior directions. Low to moderate measurement errors (%SEMeas = 10–32) were observed across muscles. Relative reliability varied from good-to-excellent (ICC = 0.63–0.99) for map volume, poor-to-excellent (ICC = 0.11–0.86) for map area, and fair-to-excellent for CoG (ICC = 0.25–0.8) across muscles. There was Bayesian evidence of equivalence (BF’s > 3) in most map outcomes between rapid and traditional maps across all muscles, supporting the validity of the rapid mapping method. Overall, rapid TMS mapping produced similar estimates of map parameters to the traditional method, however the reliability results were mixed. As mapping of non-upper limb muscles is relatively challenging, rapid mapping is a promising substitute for traditional mapping, however further work is required to refine this method.

Mammalian cell display with automated oligo design and library assembly allows for rapid residue level conformational epitope mapping

Precise epitope determination of therapeutic antibodies is of great value as it allows for further comprehension of mechanism of action, therapeutic responsiveness prediction, avoidance of unwanted cross reactivity, and vaccine design. The golden standard for discontinuous epitope determination is the laborious X-ray crystallography method. Here, we present a combinatorial method for rapid mapping of discontinuous epitopes by mammalian antigen display, eliminating the need for protein expression and purification. The method is facilitated by automated workflows and tailored software for antigen analysis and oligonucleotide design. These oligos are used in automated mutagenesis to generate an antigen receptor library displayed on mammalian cells for direct binding analysis by flow cytometry. Through automated analysis of 33930 primers an optimized single condition cloning reaction was defined allowing for mutation of all surface-exposed residues of the receptor binding domain of SARS-CoV-2. All variants were functionally expressed, and two reference binders validated the method. Furthermore, epitopes of three novel therapeutic antibodies were successfully determined followed by evaluation of binding also towards SARS-CoV-2 Omicron BA.2. We find the method to be highly relevant for rapid construction of antigen libraries and determination of antibody epitopes, especially for the development of therapeutic interventions against novel pathogens.

KEY TECHNOLOGIES FOR 1:10,000 RAPID MAPPING BASED ON GF-7 SATELLITE

Abstract. In order to solve the problems of low efficiency, high cost and long cycle of the traditional 1:10000 basic geographic information data production method (aerial photography combined with field mapping), this paper proposes a 1:10000 rapid mapping method based on GF-7 satellite data. It solves the key technical difficulties of automatic information recognition, extraction, integration and mapping of water body, building, road, vegetation, contour, and other elements. And an automatic software system of “image -> patch -> data -> map” is developed, which realizes the rapid production of 1:10000 basic geographic information data products and improves the ability of high-precision basic geographic information resource construction.

Maximum velocity projections within 30 seconds: a combination of real-time three-directional flow MRI and cross-sectional volume coverage.

This work is a proof-of-concept realization of a novel technique for rapid volumetric acquisition, reconstruction, and visualization of three-directional (3dir) flow velocities. The technique combines real-time 3dir phase-contrast (PC) flow magnetic resonance imaging (MRI) with real-time cross-sectional volume coverage. It offers a rapid examination without dependence on electrocardiography (ECG) or respiratory gating during a continuous image acquisition at up to 16 fps. Real-time flow MRI utilizes pronounced radial undersampling and a model-based nonlinear inverse reconstruction. Volume coverage is achieved by automatically advancing the slice position of each PC acquisition by a small percentage of the slice thickness. Post-processing involves the calculation of maximum intensity projections along the slice dimension resulting in six direction-selective velocity maps and a maximum speed map. Preliminary applications to healthy subjects at 3 T comprise mapping of the carotid arteries and cranial vessels at 1.0 mm in-plane resolution within 30 s as well as of the aortic arch at 1.6 mm resolution within 20 s. In conclusion, the proposed method for rapid mapping of 3dir flow velocities offers a quick assessment of the vasculature either to provide a first clinical survey or to plan for more detailed studies.

Non-equilibrium ordering of liquid crystalline (LC) films driven by external gradients in surfactant concentration

HypothesisGradients in the concentration of amphiphiles play an important role in many non-equilibrium processes involving complex fluids. Here we explore if non-equilibrium interfacial behaviors of thermotropic (oily) liquid crystals (LCs) can amplify microscopic gradients in surfactant concentration into macroscopic optical signals. ExperimentsWe use a milli-fluidic system to generate gradients in aqueous sodium dodecyl sulfate (SDS) concentration and optically quantify the dynamic ordering of micrometer-thick nematic LC films that contact the gradients. FindingsWe find that the reordering of the LCs is dominated by interfacial shearing by Marangoni flows, thus providing simple methods for rapid mapping of interfacial velocities from a single optical image and investigating the effects of confinement of surfactant-driven interfacial flows. Additionally, we establish that surface advection and surfactant desorption are the two key processes that regulate the interfacial flows, revealing that the dynamic response of the LC can provide rapid and potentially high throughput approaches to measurement of non-equilibrium interfacial properties of amphiphiles. We also observe flow-induced assemblies of microparticles to form at the LC interface, hinting at new non-equilibrium approaches to microparticle assembly. We conclude that dynamic states adopted by LCs in the presence of surfactant concentration gradients provide new opportunities for engineering complex fluids beyond equilibrium.

In situ laser ablation Lu–Hf geochronology of garnet across the Western Gneiss Region: campaign-style dating of metamorphism

The development of in situ laser ablation Lu–Hf geochronology of apatite, xenotime and garnet has opened avenues to quickly and directly date geological processes. We demonstrate the first use of campaign-style in situ Lu–Hf geochronology of garnet across the high- to ultrahigh-pressure Western Gneiss Region in Norway. Mafic eclogites from this region have been the focus of much work, and were clearly formed during continental subduction during the Caledonian Orogeny. However, abundant quartzofeldspathic and pelitic lithologies record a more complex history, with some preserving polymetamorphic age data, and most containing no indication of high-pressure mineral assemblages formed during subduction. Twenty metapelitic and felsic samples spanning 160 lateral kilometres across the Western Gneiss Region have been analysed using garnet Lu–Hf geochronology. The results reveal Caledonian ages for the majority of the garnets, suggesting that some quartzofeldspathic and metapelitic lithologies were reactive and grew garnet during high- to ultrahigh-pressure metamorphism. However, two ultrahigh-pressure eclogite locations, Verpeneset and Fjørtoft, preserve both Caledonian and Neoproterozoic-aged garnets. Despite significant uncertainties on some of the Lu–Hf geochronological ages, laser ablation Lu–Hf efficiently identifies the polymetamorphic history of parts of the Western Gneiss Region, illustrating the effectiveness of this novel analytical method for rapid mapping of metamorphic ages. Supplementary material: All laser ablation Lu–Hf geochronological data for the garnets analysed in this study are available at https://doi.org/10.6084/m9.figshare.c.5715453 Thematic collection: This article is part of the Caledonian Wilson cycle collection available at: https://www.lyellcollection.org/cc/caledonian-wilson-cycle

Co-seismic landslide detection after M 7.4 earthquake on June 23, 2020, in Oaxaca, Mexico, based on rapid mapping method using high and medium resolution synthetic aperture radar (SAR) images

Co-seismic landslide detection after M 7.4 earthquake on June 23, 2020, in Oaxaca, Mexico, based on rapid mapping method using high and medium resolution synthetic aperture radar (SAR) images

IDENTIFICATION OF TEMPORARY SURFACE WATER USING SENTINEL-1 SAR DATA, CASE STUDY: SENTANI FLASH FLOODING, INDONESIA

Abstract. Temporary surface water monitoring can provide accurate and reliable information about the spatio-temporal level of surface water. This is very important for various environmental applications, such as flood monitoring. Remote sensing data such as Synthetic Aperture Radar (SAR) is very useful for a large-scale flood monitoring. SAR sensors offer clear advantages by providing their own sources of illumination, thus being able to operate in nearly all-weather/day-night conditions. About 30% disasters which occurred in Indonesia are floods. This hazard has become a recurring disaster that takes place annually. A massive flash flood struck Sentani in the Jayapura Regency in the province of Papua, Indonesia on 16 March 2019, causing 104 deaths. The objective of this work is thus to map temporary surface water (flood) of the Sentani flash flooding event in Indonesia using Sentinel-1 SAR imagery. Sentinel-1 IW GRD and SLC (dual polarimetry) on the event period were used. With two types of Sentinel-1 data, this research produced temporary surface water map using rapid mapping method and SAR polarimetry method. Comparing the results, the similarity of SAR polarimetry method to rapid mapping method is about 39%. Based on reference data, rapid mapping result show better accuracy (82%) than SAR polarimetry method (62%). In addition, processing SLC data needs longer time and higher performance than processing GRD data. Thus, for rapid mapping, it is better to use only Sentinel-1 GRD data.

Aerial magnetic mapping with an unmanned aerial vehicle and a fluxgate magnetometer: a new method for rapid mapping and upscaling from the field to regional scale

ABSTRACTMagnetic measurements with an unmanned aerial vehicle are ideal for filling the gap between ground and airborne magnetic surveying. However, to obtain accurate aeromagnetic data, the compensation of magnetic effects of the unmanned aerial vehicle is a challenge. Typically, scalar magnetometers are towed several metres under the unmanned aerial vehicle to minimize its magnetic field. In this study, a fluxgate three‐component magnetometer is attached 42 cm in front of the unmanned aerial vehicle at the tip of a composite pipe. Using a scalar calibration, the sensor can be calibrated, and the permanent and induced magnetic fields of the unmanned aerial vehicle can be compensated. The contributions of the magnetic measurements at different altitudes to the unmanned aerial vehicle results were tested over an area of 1 km² in the Northern Vosges Mountains. The area is located in a hamlet surrounded by a forest where few geological outcrops are observed. Three magnetic surveys of the same area are obtained at different altitudes: 100, 30 and 1 m above the ground. The unmanned aerial vehicle magnetic data are compared with a helicopter aeromagnetic survey at 300 m above the ground and a ground magnetic survey using upward continuations of the maps to compare the results. The magnetic maps (300, 100, 30 and 1 m above the ground) show very different magnetic anomaly patterns (e.g. amplitude, shape, wavelength and orientation). The magnetic data at different altitudes improve the understanding of the geology from the local to more general scales.

Rapid flood inundation mapping by differencing water indices from pre- and post-flood Landsat images

Following flooding disasters, satellite images provide valuable information required for generating flood inundation maps. Multispectral or optical imagery can be used for generating flood maps when the inundated areas are not covered by clouds. We propose a rapid mapping method for identifying inundated areas based on the increase in the water index value between the pre- and post-flood satellite images. Values of the Normalized Difference Water Index (NDWI) and Modified NDWI (MNDWI) will be higher in the post-flood image for flooded areas compared to the pre-flood image. Based on a threshold value, pixels corresponding to the flooded areas can be separated from non-flooded areas. Inundation maps derived from differencing MNDWI values accurately captured the flooded areas. However the output image will be influenced by the choice of the pre-flood image, hence analysts have to avoid selecting pre-flood images acquired in drought or earlier flood years. Also the inundation maps generated using this method have to be overlaid on the post-flood satellite image in order to orient personnel to landscape features. Advantages of the proposed technique are that flood impacted areas can be identified rapidly, and that the pre-existing water bodies can be excluded from the inundation maps. Using pairs of other satellite data, several maps can be generated within a single flood which would enable emergency response agencies to focus on newly flooded areas.

Rapid Mapping for Simple Flood Mitigation Using Commercial Drone at Way Galih Village, Lampung, Indonesia

The process to alleviate flood risk, especially flood from a river that occurs excessively in Indonesia, requires a new approach. The attempt to reduce the risk along with the development of technology is by utilising commercial drones and rapid mapping methods for mapping flood plain area. With the rapid mapping method, the flood mitigation process in the village area can be done quickly. The activity carried out was to map the location of possible flooding in the Way Galih Village and case analysis to determine the location of the biogas digester together with the village government authority. The results of the study show that the data acquisition process and aerial photo processing can be carried out within one day and the village policymaker can quickly make policies and decisions about where to place the biogas digester.

A Novel Method for Rapid Mapping of the Spatial Intensity of Influenza Epidemics

ObjectiveUsing the epidemic of influenza type A in 2016 in Australia, we demonstrated a simple but statistically sound adaptive method of automatically representing the spatial intensity and evolution of an influenza epidemic that could be applied to a laboratory surveillance count data stream that does not have a denominator.IntroductionSurveillance of influenza epidemics is a priority for risk assessment and pandemic preparedness. Mapping epidemics can be challenging because influenza infections are incompletely ascertained, ascertainment can vary spatially, and often a denominator is not available. Rapid, more refined geographic or spatial intelligence could facilitate better preparedness and response.MethodsWeekly counts of persons with laboratory confirmed influenza type A infections in Australia in 2016 were analysed by 86 sub-state geographical areas. Weekly standardised epidemic intensity was represented by a z-score calculated using the standard deviation of below-median counts in the previous 52 weeks. A geographic information system was used to present the epidemic progression.ResultsThere were 79,628 notifications of influenza A infections included. Of these, 79,218 (99.5%) were allocated to a geographical area. The maps indicated areas of elevated epidemic intensity across Australia by week and area, that were consistent with the observed start, peak and decline of the epidemic when compared with weekly counts aggregated at the state and territory level. An example is shown in Figure 1.ConclusionsThe methods could be automated to rapidly generate spatially varying epidemic intensity maps using a surveillance data stream. This could improve local level epidemic intelligence in a variety of settings and for other diseases. It may also increase our understanding of geographic epidemic dynamics.

ESTABLISHING A TEACHING PRACTICE PROCESS THROUGH TRAIN THE TRAINER PROGRAM IN THE SINABUNG VOLCANO’s EXCLUSION ZONE: A QUALITATIVE CASE STUDY IN MARDINGDING VILLAGE AT THE DISTRICT OF KARO

AbstractThis paper discusses the outcomes resulted from efforts in optimizing the implementation of a teaching practice process in a current/post-disaster area. The purpose of this study is to implement the Train-the-Trainer program for local youth as educational agents to pupils in Mardingding Village and in other villages around those affected by the disaster so that the learning spirit of students is maintained. Based on the data analysis using the rapid mapping method with the Rapid Spiral Boehm model, it can be concluded that the implementation and existence of the Train the Trainer program has a positive influence on students' interest in learning activities that are not only focused on activities within the school but also outside the school. The relatively good achievement of learning outcomes after the implementation of the train the trainer program underscores interesting findings in which regions experiencing disasters can still meet learning outcomes with all the inherent limitations of the condition of the affected area. This applied research has limitations, namely focusing on qualitative data analysis so that the interpretation of the findings obtained also has relatively specific implications for the coverage of the Mardingding Village area in the Tiganderket District in Karo District.Keywords: Train the Trainer Program, Teaching Practice Process, Disaster Area, Mardingding Village, Rapid Spiral Boehm.

线状地物快速图斑化方法研究

线状地物快速图斑化方法研究

Next‐Generation Sequencing for Identification of EMS‐Induced Mutations in Caenorhabditis elegans

Forward genetic analysis using chemical mutagenesis in model organisms is a powerful tool for investigation of molecular mechanisms in biological systems. In the nematode, Caenorhabditis elegans, mutagenesis screens using ethyl methanesulfonate (EMS) have led to important insights into genetic control of animal development and physiology. A major bottleneck to this approach is identification of the causative mutation underlying a phenotype of interest. In the past, this has required time-consuming genetic mapping experiments. More recently, next-generation sequencing technologies have allowed development of new methods for rapid mapping and identification of EMS-induced lesions. In this unit we describe a protocol to map and identify EMS-induced mutations in C. elegans. © 2017 by John Wiley & Sons, Inc.

Rapid Mapping Method Based on Free Blocks of Surveys

Abstract While producing large-scale larger than 1:2000 maps in cities or towns, the obstruction from buildings leads to difficult and heavy tasks of measuring mapping control points. In order to avoid measuring the mapping control points and shorten the time of fieldwork, in this paper, a quick mapping method is proposed. This method adjusts many free blocks of surveys together, and transforms the points from all free blocks of surveys into the same coordinate system. The entire surveying area is divided into many free blocks, and connection points are set on the boundaries between free blocks. An independent coordinate system of every free block is established via completely free station technology, and the coordinates of the connection points, detail points and control points in every free block in the corresponding independent coordinate systems are obtained based on poly-directional open traverses. Error equations are established based on connection points, which are determined together to obtain the transformation parameters. All points are transformed from the independent coordinate systems to a transitional coordinate system via the transformation parameters. Several control points are then measured by GPS in a geodetic coordinate system. All the points can then be transformed from the transitional coordinate system to the geodetic coordinate system. In this paper, the implementation process and mathematical formulas of the new method are presented in detail, and the formula to estimate the precision of surveys is given. An example has demonstrated that the precision of using the new method could meet large-scale mapping needs.

Rapid mapping of urban development from historic Ordnance Survey maps: An application for pluvial flood risk in Worcester

Between 2004 and 2008 the city of Worcester, UK experienced a number of pluvial flood events. The causes of this kind of flooding are the topic of ongoing research. This paper describes a study that aimed to investigate the urban development of Worcester over time in relation to the location of recorded pluvial flood incidents. A novel rapid mapping method has been developed to derive urban development over five time periods between 1886 and 1995 from scanned Ordnance Survey historical maps. The technique compared well with manual digitisation results with k-hat values ranging from 0.67 to 0.87 for the land use maps created for different time periods. The technique performed least well for the oldest map series, due to misclassification of the abundant symbols and annotation. The method will be particularly beneficial for investigation of town/city development in time over large areas. The resulting map of urban development in Worcester (scale 1:20,000) shows that almost half of the recorded pluvial flood incidents occurred in areas developed between 1956 and 1975, which contradicts local belief that an outdated (Victorian) drainage system causes most of the problems. The quality of the post World War II developments is more likely to be a source for concern.

Fast Mapping of Biomolecular Interfaces by Random Spin Labeling (RSL)

Random spin labeling (RSL) is a method for rapid mapping of biomolecular interaction surfaces using an interaction partner with SL and an interaction partner enriched in 13C or 15N nuclei for paramagnetic relaxation enhanced NMR-based detection. The SL reaction is conducted in a manner resulting in a heterogeneous reaction product consisting of different populations of the protein carrying a varying number of spin labels at different positions. Preparation of the paramagnetic probe is complete within a few hours and hence much faster than site selective SL. RSL is applicable to tightly interacting systems but shows its particular strength when applied to systems involving weak or transient contacts.

Investigating the spatial micro-epidemiology of diseases within a point-prevalence sample: a field applicable method for rapid mapping of households using low-cost GPS-dataloggers

SummaryPoint-prevalence recording of the distribution of tropical parasitic diseases at village level is usually sufficient for general monitoring and surveillance. Whilst within-village spatial patterning of diseases exists, and can be important, mapping infected cases in a household-by-household setting is arduous and time consuming. With the development of low-cost GPS-data loggers (< £40) and available GoogleEarthTM satellite imagery, we present a field-applicable method based on crowdsourcing for rapid identification of infected cases (intestinal schistosomiasis, malaria and hookworm) by household. A total of 126 mothers with their 247 preschool children from Bukoba village (Mayuge District, Uganda) were examined with half of these mothers given a GPS-data logger to walk home with, returning the unit the same day for data off-loading, after which, households were assigned GPS coordinates. A satellite image of Bukoba was annotated with households denoting the infection status of each mother and child. General prevalence of intestinal schistosomiasis, malaria and hookworm in mothers and children was: 27.2 vs 7.7%, 28.6 vs 87.0% and 60.0 vs 22.3%, respectively. Different spatial patterns of disease could be identified likely representing the intrinsic differences in parasite biology and interplay with human behaviour(s) across this local landscape providing a better insight into reasons for disease micro-patterning.

Spatial variation of Anopheles-transmitted Wuchereria bancrofti and Plasmodium falciparum infection densities in Papua New Guinea

The spatial variation of Wuchereria bancrofti and Plasmodium falciparum infection densities was measured in a rural area of Papua New Guinea where they share anopheline vectors. The spatial correlation of W. bancrofti was found to reduce by half over an estimated distance of 1.7 km, much smaller than the 50 km grid used by the World Health Organization rapid mapping method. For P. falciparum, negligible spatial correlation was found. After mass treatment with anti-filarial drugs, there was negligible correlation between the changes in the densities of the two parasites.