Range Of Responses Research Articles

Portable photoelectrochemical immunoassay with micro-electro-mechanical-system for alpha-fetoprotein in hepatocellular carcinoma

Early detection of cancer has a profound impact on patient survival and treatment outcomes considering high treatment success rates and reduced treatment complexity. Here, we developed a portable photoelectrochemical (PEC) immune platform for sensitive testing of alpha-fetoprotein (AFP) based on Pt nanocluster (Pt NCs) loaded defective-state g-C3N4 photon-electron transducers. The broad forbidden band structure of g-C3N4 was optimized by the nitrogen doping strategy and additional homogeneous porous structure was introduced to further enhance the photon utilization. In addition, the in-situ growth of Pt NCs provided efficient electron transfer catalytic sites for sacrificial agents, which were used to further improve the sensitivity of the sensor. Efficient photoelectric conversion under a hand-held flashlight was determined by the geometry of the transducer and the energy band design, and the portable design of the PEC sensor was realized. The developed sensing platform exhibited a wide linear response range (0.1–50 ng mL−1) and low limit of detection (0.043 ng mL−1) for AFP under optimum conditions. This work provides a new idea for designing portable PEC biosensing platforms to meet the current mainstream POC testing needs.

TMR-high-temperature superconductor composite magnetic sensor and its performance optimization

Tunnel magnetoresistance (TMR), recognized for its high sensitivity and low power consumption, holds significant promise in the domain of weak magnetic field detection. Using superconducting materials as magnetic concentrators can achieve several hundred to even a thousandfold amplification of magnetic fields, making it one of the most effective approaches to enhance the magnetic field resolution of TMR sensors. This paper utilized the flip-chip bonding process to integrate TMR with the high-temperature superconductor YBCO (YBa2Cu3O7-δ), and successfully developed TMR-YBCO composite magnetic sensor. Building upon this foundation, through optimization of the fabrication process and the pioneering use of a structural design incorporating the filling of annular holes with superconducting concentrators, the sensitivity of the sensor was further enhanced. Finally, the magnetic field resolution was increased by 1030 times compared to TMR sensors, reached to 2.9pT/Hz1/2 at 1 Hz. Simultaneously, results from frequency band testing indicated excellent frequency band characteristics, with a frequency response range exceeding kHz.

Significant enhancement in the photoelectronic properties of SnI4 via pressure-tailored phase engineering

Constantly developing superior functional materials with fascinating photoelectric properties is crucial for advancing the optoelectronics industry. Here, we demonstrate a feasible strategy to significantly improve the photoelectric properties of SnI4 through employing high pressure phase engineering. With increasing pressure, the photocurrent response of SnI4 undergoes sequential enhancement and the maximum photocurrent of SnI4 is five orders of magnitude higher than that observed at initial pressure under Xe-lamp illumination. Surprisingly, the photoelectric spectral response range was expanded from visible region to near-infrared waveband (1650 nm) upon successive compression. These remarkable enhancements in photoelectric activities are attributed to the pressure-induced semiconducting cubic phase to metal triclinic phase transition in SnI4. The occurrence of the metal phase improves the charge carriers transfer ability and photon absorption in a wider spectral range. Our findings provide some valuable insights and an avenue for regulating the photoelectric properties of materials by manipulating the phase composition with pressure.

Catholic Pastoral and Prophetic Responses to a Secularizing Landscape

The purpose of this article is to offer a retrieval of some major themes in the writings of Ro­mano Guardini and Ivan Illich, two twentieth century Catholic priests who responded to secularization in ways that could be described as both pastoral and prophetic. Guardini tended towards a more pas­toral emphasis, while Illich’s stance was more prophetic. Guardini exemplified a Catholic way to bridge the gap often experienced between faith, life and culture. Illich demonstrated how a Catholic can ad­dress their culture in a way that is both challenging and fertile and does so in penetrating observations about contemporary secular professions and preoccupations. The article has three parts. In part one, I sketch a range of responses to Christianity that have emerged in an increasingly secular culture, as well as the types of response that Christians have made to such a culture. In parts two and three a repre­sentative sample of the writings of Guardini and Illich is drawn on and analysed, together with a range of secondary literature on Guardini and Illich, in order to explain the nature and the role of four key themes (in each case) within their overall outlook. For Guardini, in part two, these are Catholic worldview, lit­urgy, providence and cultural critique. For Illich, in part three, I examine his notion of the mission of the Church, his treatment of schooling, his understanding that modernity is a perversion of Christianity, and his advocacy of friendship as a healing and liberating mode of engaging the world

Recent tree diversity increase in NE Iberian forests following intense management release: A task for animal‐dispersed and drought‐tolerant species

Abstract Under increasing human‐related threats to forests, many studies suggest that increasing tree species diversity may boost forest resilience by enhancing the range of species' responses to disturbances. However, it remains unclear whether passive or active forest management strategies should be applied to increase tree diversity. This issue would benefit from investigating which management and environmental factors, together with species' functional traits, influence temporal changes in tree species diversity. We explored the influence of the bioclimatic region, land‐use history, forest cover, protection, management, forest structure and changes in temperature and precipitation, to explain tree species diversity changes in NE Iberian forests, by comparing 3141 plots from the Spanish National Forest Inventory sampled between 1989 and 2016. Moreover, we assessed which species' functional traits (dispersal habit, drought and shade tolerance) were most relevant for diversity changes. After 27 years, tree species richness and diversity moderately increased in the tree and regeneration layers. This trend occurred mostly in long‐established, non‐recently managed forests and in those with a lower initial basal area. Increasing temperature had negative effects for diversity increase in the tree layer but positive for the regeneration compartment, while decreasing precipitation showed the opposite effects. Tree species with higher drought tolerance, and especially those animal‐dispersed ones arriving from the regional pool, mostly contributed to the local diversity increase. This pattern occurred in all forest types, although the taxonomic array of species varied. Synthesis and applications. The main drivers influencing the passive increase in tree species diversity suggest a primary role of diminishing forest exploitation in this recovery process, fine‐tuned by climatic changes. This ecological scenario has particularly favoured animal‐dispersed tree species with higher drought tolerance, which mostly led the diversity increase. A higher presence of such highly mobile and drought‐tolerant species can be crucial to increase functional diversity and, ultimately, increase forest resilience under future scenarios of greater aridity. In light of these results, management strategies should continue fostering the restoration of diversity in once intensively exploited forests while ensuring the maintenance of the already gained tree species diversity.

Turning Antibodies into Ratiometric Bioluminescent Sensors for Competition-Based Homogeneous Immunoassays.

Here we present LUCOS (Luminescent Competition Sensor), a modular and broadly applicable bioluminescent diagnostic platform enabling the detection of both small molecules and protein biomarkers. The construction of LUCOS sensors entails the covalent and site-specific coupling of a bioluminescent sensor component to an analyte-specific antibody via protein G-mediated photoconjugation. Target detection is accomplished through intramolecular competition with a tethered analyte competitor for antibody binding. We established two variants of LUCOS: an inherent ratiometric LUCOSR variant and an intensiometric LUCOSI version, which can be used for ratiometric detection upon the addition of a split calibrator luciferase. To demonstrate the versatility of the LUCOS platform, sensors were developed for the detection of the small molecule cortisol and the protein biomarker NT-proBNP. Sensors for both targets displayed analyte-dependent changes in the emission ratio and enabled detection in the micromolar concentration range (KD,app = 16-92 μM). Furthermore, we showed that the response range of the LUCOS sensor can be adjusted by attenuating the affinity of the tethered NT-proBNP competitor, which enabled detection in the nanomolar concentration range (KD,app = 317 ± 26 nM). Overall, the LUCOS platform offers a highly versatile and easy method to convert commercially available monoclonal antibodies into bioluminescent biosensors that provide a homogeneous alternative for the competitive immunoassay.

Studies on the Aptasensor Miniaturization for Electrochemical Detection of Lead Ions.

Lead poses severe effects on living organisms, and since Pb2+ ions tend to accumulate in different organs, it is crucial to monitor Pb2+ concentration in samples such as water and soil. One of the approaches is the utilization of biosensors combined with aptamer-based layers for the electrochemical detection of lead ions. Herein, we present the studies of applying miniaturized screen-printed transducers as solid surfaces to fabricate aptamer layers. As the research is the direct continuation of our previous studies regarding the use of gold disk electrodes, the working parameters of elaborated aptasensors were defined, including the range of linear response (10-100 nM), selectivity as well as stability, regeneration, and feasibility of application for the analysis of real samples. This was achieved using voltammetric techniques including cyclic and square-wave voltammetry in the presence of methylene blue redox indicator.

Enhanced CO2 Electrolysis Through Mn Substitution Coupled with Ni Exsolution in Lanthanum Calcium Titanate Electrodes.

In this study, perovskite oxides La0.3Ca0.6Ni0.05MnxTi0.95- xO3- γ (x = 0, 0.05, 0.10) are investigated as potential solid oxide electrolysis cell cathode materials. The catalytic activity of these cathodes toward CO2 reduction reaction is significantly enhanced through the exsolution of highly active Ni nanoparticles, driven by applying a current of 1.2 A in 97% CO2 - 3% H2O. The performance of La0.3Ca0.6Ni0.05Ti0.95O3-γ is notably improved by co-doping with Mn. Mn dopants enhance the reducibility of Ni, a crucial factor in promoting the in situ exsolution of metallic nanocatalysts in perovskite (ABO3) structures. This improvement is attributed to Mn dopants enabling more flexible coordination, resulting in higher oxygen vacancy concentration, and facilitating oxygen ion migration. Consequently, a higher density of Ni nanoparticles is formed. These oxygen vacancies also improve the adsorption, desorption, and dissociation of CO2 molecules. The dual doping strategy provides enhanced performance without degradation observed after 133h of high-temperature operation, suggesting a reliable cathode material for CO2 electrolysis.

Unraveling Links between Chronic Inflammation and Long COVID: Workshop Report.

As COVID-19 continues, an increasing number of patients develop long COVID symptoms varying in severity that last for weeks, months, or longer. Symptoms commonly include lingering loss of smell and taste, hearing loss, extreme fatigue, and "brain fog." Still, persistent cardiovascular and respiratory problems, muscle weakness, and neurologic issues have also been documented. A major problem is the lack of clear guidelines for diagnosing long COVID. Although some studies suggest that long COVID is due to prolonged inflammation after SARS-CoV-2 infection, the underlying mechanisms remain unclear. The broad range of COVID-19's bodily effects and responses after initial viral infection are also poorly understood. This workshop brought together multidisciplinary experts to showcase and discuss the latest research on long COVID and chronic inflammation that might be associated with the persistent sequelae following COVID-19 infection.

Flexible and breathable textile-based multi-functional strain sensor for compressive sensing, electromagnetic shielding, and electrical heating

The textile-based sensors have drawn increasing attention in wearable smart electronics, owing to the comfort, breathability, and elasticity. However, the low compressive strain response range, poor thermal comfort and electromagnetic shielding performance hinder severely the application of textile-based sensors in the complex environments. To address these issues, we chose a weft-flat knitted textile with a large gap as a flexible substance, graphene nanosheets (GNS), and Ag nanowires (Ag NWs) as conductive fillers, and treated the textile with a simple dip coating strategy to obtain TPU/GNS/Ag NWs@textile (TAG@textile). The prepared TAG@textile sensor not only exhibits 90% compressive strain response range and high sensitivity (GF=9.25), but also exhibits an efficient electromagnetic shielding performance of 61.68 dB. In addition, the TAG@textile sensor has excellent Joule thermal performance (it can reach 104 °C at a low voltage of 4 V). In brief, this work integrates human motion detection, electromagnetic shielding, and electrical heating, showing a great potential in future multifunctional wearable electronic devices.

Reducing Interfacial Losses in Solution-Processed Integrated Perovskite-Organic Solar Cells.

Low bandgap organic semiconductors have been widely employed to broaden the light response range to utilize much more photons in the inverted perovskite solar cells (PSCs). However, the serious charge recombination at the heterointerface contact between perovskite and organic semiconductors usually leads to large energy loss and limits the device performance. In this work, a titanium chelate, bis(2,4-pentanedionato) titanium(IV) oxide (C10H14O5Ti), was directly used as an interlayer between the perovskite layer and organic bulk heterojunction layer for the first time. Impressively, it was found that C10H14O5Ti can not only increase the surface potential of perovskite films but also show a positive passivation effect toward the perovskite film surface. Drawing from the above function, a smoother perovskite active layer with a higher work function was realized upon the use of C10H14O5Ti. As a result, the C10H14O5Ti-modified integrated devices show lower interfacial loss and obtain the best power conversion efficiency (PCE) of up to 20.91% with a high voltage of 1.15 V. The research offers a promising strategy to minimize the interfacial loss for the preparation of high-performance perovskite solar cells.

Trap-assisted monolayer ReSe2/Si heterojunction with high photoconductive gain and self-driven broadband photodetector.

The development of photodetectors is crucial in fields such as optical communication, image sensing, medical devices and military equipment, where high sensitivity is paramount. We fabricated an ambipolar photodiode using monolayer triclinic ReSe2, synthesized by chemical vapor deposition on p-type Si substrate. The photodetector has a broadband response range from 405 to 1100 nm. The device exhibits high sensitivity to NIR radiation with a high Iph/Idark (ON/OFF) ratio of 5.8 × 104, responsivity (R) of 465 A/W, and specific detectivity (D) of 4.8 × 1013 Jones at open circuit voltage (Voc), indicating photovoltaic behavior. Our ReSe2/Si heterojunction photodetector also exhibits low dark current of 1.4 × 10-9 A and high external quantum efficiency (EQE) of 54368.2% for 1060 nm at -3 V, demonstrating a photoconductive gain. The maximum responsivity (R = 465 A/W) can be achieved at -3 V reverse bias under 1060 nm. The device has a high ideality factor (4.8) and power coefficient (α = 0.5), indicating the presence of interface and sub-gap states that enhance device responsivity at lower illumination intensities by re-exciting trapped carriers into the conduction band. Our results offer important insights into the underlying photo-physics of the ReSe2/Si heterojunction and propose promising avenues for developing advanced broadband photodetectors of high performance.

Development of a Competitive Chemiluminescent Assay for Quantitative Determination of TP53 Fusion Protein Using Reagent Strips.

Recent studies have shown that almost half of all cancers occur due to DNA damage. For the early diagnosis of cancer, a highly sensitized and swift identification for TP53 is needed since the corresponding TP53 protein is effectively recognized as "the guardian of the genome." To improve the detection sensitivity, numerous analytical methods were previously used for the determination of the TP53 protein, including denaturing high-performance liquid chromatography and enzyme-linked immunosorbent assay (ELISA). Currently, immunochromatographic tests (ICTs) that are simple to use, stable over time, and show low interference are regarded as valuable tools for the quick screening of food and environmental monitoring along with clinical diagnosis. ICTs often have limited sensitivity even if a variety of novel reporters possessing optimum photostability and improved brightness are used as signal-intensity reporters. Compared with N-(4-aminobutyl)-N-(ethylisoluminol) or luminol, a novel luminescent probe, 2',6'-diMethyl-4'-(N-succiniMidyloxycarbonyl) phenyl-10-sulfopropylacridiniuM-9-carboxylate (NSP-DMAE-NHS) has achieved a much higher efficiency, improvement in the biosensor's performance, and amplification of the signal without causing any damage to the biomolecule in terms of its biochemical activity. In this study, the reagent strip method was initially used to detect TP53 fusion protein by combining the advantages of NSP-DMAE-NHS and immunochromatography. In our experiment, the control and study lines on the strips were immobilized through HRP-conjugated goat anti-rabbit IgG and TP53 antigen, respectively. The optimized concentration of the anti-TP53 antibody-NSP-DMAE-NHS immunoconjugates was then added to the TP53 antigen samples. After, the test strips were inserted and left in the aforementioned buffer solution for an additional 20 min. Finally, a lab-made luminous measurement device was used to analyze the corresponding control and study lines on the strips. Under optimized conditions, this method was found to be ultrasensitive, with a wide range of linear responses from 0.0008 ng mL-1 to 1 µg mL-1 and a limit of detection of 0.0008 ng mL-1 (0.013 pM). Thus, a novel competitive chemiluminescent assay based on reagent strips was established for the determination of the TP53 fusion proteins. The strategy has potential applications for ultrasensitive detection in the early diagnosis of cancer.

Oxygen-defected WO3-OVs@Bi2MoO6 S-scheme micro flowers heterojunctions with promoted photocatalytic degradation of tetracycline

The synergy between surface oxygen vacancies (OVs) and heterojunction engineering holds promising potential for substantially enhancing photoelectric conversion efficiency through the optimization of photogenerated charge carrier transport. Herein, novel WO3-OVs@Bi2MoO6 S-scheme heterostructures are synthesized via a simple route for degradation of tetracycline hydrochloride (TC) using visible light. The S-scheme charge transfer mode markedly enhances the spatial separation and preservation of high-energy electrons/holes on Bi2MoO6 (reduction) and WO3-OVs (oxidation), consequently providing WO3-OVs@Bi2MoO6 photocatalysts with an improved redox capability. The optimal WO3-OVs@Bi2MoO6 heterojunction demonstrates enhanced visible-light-driven photocatalytic degradation of tetracycline, exhibiting 4.86-fold and 4.17-fold higher efficiency compared to WO3-OVs and Bi2MoO6, respectively, with superior stability and reusability. Confirmation of the existence of the S-scheme charge transfer mechanism and oxygen vacancies (OVs) is achieved through a combination of density functional theory (DFT) calculations and in-situ X-ray photoelectron spectroscopy (XPS), substantiating the enhancement of charge separation and redox capability. Specifically, the presence of oxygen vacancies (OVs) on the surface of WO3 nanofibers results in a reduction of the band gap energy due to the defect levels, consequently promoting increased electron conduction. Furthermore, the abundance of oxygen vacancies (OVs) expands the photo response range and facilitates charge transfer at the interface. This research opens new avenues for the successful photocatalytic degradation of tetracycline (TC) by integrating hierarchical S-scheme heterojunction design and cutting-edge defect engineering in photocatalytic heterojunctions.

3D Printing of Capacitive Pressure Sensors with Tuned Wide Detection Range and High Sensitivity Inspired by Bio-Inspired Kapok Structures.

Flexible pressure sensors have drawn considerable attention for their potential applications as electronic skins with both sensitivity and pressure response range. Although the introduction of surface microstructures effectively enhances sensitivity, the confined volume of their compressible structures results in a limited pressure response range. To address this issue, a biomimetic kapok structure is proposed and implemented for constructing the dielectric layer of flexible capacitive pressure sensors employing 3D printing technology. The structure is designed with easily deformable concave and rotational structures, enabling continuous deformation under pressure. This design results in a significant expansion of the pressure response range and improvement in sensitivity. Further, the study purposively analyses crucial parameters of the devised structure that affect its compressibility and stability. These include the concave angle θ, height ratio d1/d2, rotation angle α, and width k. As a result, the ultimate pressure sensors demonstrate remarkable features such as high sensitivity (≈2.38kPa-1 in the range of 0-10kPa), broad detection range (734kPa), fast response time (23ms), and outstanding pressure resolution (0.4% at 500kPa). This study confirms the viability of bionic structures for flexible sensors, and their potential to expand the scope of wearable electronic devices.

NIR-Mediated Cu2O/Au Nanomotors for Synergistically Treating Hepatoma Carcinoma Cells.

We presented a NIR-driven Janus Cu2O/Au nanomotor. The nanomotor has a truncated octahedral structure. By asymmetric Au evaporation, the light response range of Cu2O nanomotor is extended to near-infrared range, and the speed of Cu2O/Au nanomotors under NIR is significantly increased. In promoting apoptosis of hepatocellular carcinoma, except the nanotoxicity of Cu2O itself, the Au layer enhances the photothermal properties, allowing Cu2O/Au nanomotors to induce apoptosis in hepatocellular carcinoma cells by heating them. On the other hand, a Schottky barrier formed at the interface of Cu2O and Au, preventing the recombination of electrons, which makes more electrons react with biomolecules to produce toxic ROS to kill hepatocellular cells. The killing rate of hepatocellular carcinoma cells reached 87 % by the combined effect of nanotoxicity inhibition of proliferation and photothermal & photodynamic therapy (PTT & PDT). Nanomotors in combination with multiple approaches are explored as a new treatment to tumor in this article.

Beetle‐Inspired Gradient Slant Structures for Capacitive Pressure Sensor with a Broad Linear Response Range

AbstractFlexible pressure sensors with broad linearity range and excellent sensor‐to‐sensor uniformity have attracted unprecedented attention in the electronic skins, human–machine interfaces, and environmental monitoring. However, challenges including poor sensor‐to‐sensor uniformity owing to the randomness of the used nanomaterials or porous structures and saturated response that leads to a restricted linearity range because of structural stiffening have been yet addressed. Herein, a novel dielectric layer based on beetle‐inspired gradient slant structures (GSS) is proposed to endow capacitive pressure sensors with extensive linearity range and excellent sensor‐to‐sensor uniformity. The excellent compressibility of the GSS due to the bending deformation of the slant pillars significantly enhances sensor sensitivity. The broad linearity range comes from the compensation of contact area during sequential contact of the GSS dielectric layer from tall to low slant pillars with electrodes. The high sensor‐to‐sensor uniformity is ascribed to the excellent batch‐to‐batch consistency of prepared GSS via 3D printing‐based fabrication process. Moreover, the proposed GSS‐based pressure sensors present rapid response/recovery, low detection limit, excellent dynamic response, negligible hysteresis, and outstanding long‐term stability. Finally, the excellent applicabilities of proposed capacitive pressure sensors in diverse scenarios including external pressure stimuli detection, a flexible perception array, and a smart insole system are demonstrated.

The interplay between the circadian clock and abiotic stress responses mediated by ABF3 and CCA1/LHY

Climate change is a global concern for all life on our planet, including humans and plants. Plants' growth and development are significantly affected by abiotic stresses, including adverse temperature, inadequate or excess water availability, nutrient deficiency, and salinity. The circadian clock is a master regulator of numerous developmental and metabolic processes in plants. In an effort to identify new clock-related genes and outputs through bioinformatic analysis, we have revealed that CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) play a crucial role in regulating a wide range of abiotic stress responses and target ABSCISIC ACID RESPONSIVE ELEMENTS-BINDING FACTOR3 (ABF3), a key transcription factor in the plant hormone Abscisic acid (ABA)-signaling pathway. Specifically, we found that CCA1 and LHY regulate the expression of ABF3 under diel conditions, as well as seed germination under salinity. Conversely, ABF3 controls the expression of core clock genes and orchestrates the circadian period in a stress-responsive manner. ABF3 delivers the stress signal to the central oscillator by binding to the promoter of CCA1 and LHY. Overall, our study uncovers the reciprocal regulation between ABF3 and CCA1/LHY and molecular mechanisms underlying the interaction between the circadian clock and abiotic stress. This finding may aid in developing molecular and genetic solutions for plants to survive and thrive in the face of climate change.

Biomaterial‐Facilitated Local Delivery of Stem Cell‐Derived Small Extracellular Vesicles: Perspectives in Surgical Therapy

AbstractStem cell‐derived small extracellular vesicles (sEVs) have shown tremendous potential in regenerative medicine in recent years. These sEVs exert multiple therapeutic effects comparable or superior to their parental stem cells while avoiding the limitations of stem cell‐based therapy. The two routine modalities of sEV administration are systemic injection and local delivery. Various biomaterials are developed to assist their local delivery to improve the targeting of organs, minimize nonspecific accumulation in vital organs, and ensure the protection and release of sEVs. This comprehensive, up‐to‐date, specialty‐specific, disease‐oriented, and preclinical review is presented to expound on the surgical application and potential of stem cell‐derived sEVs. The local delivery of stem cell‐derived sEVscan treat numerous diseases encountered in orthopedic surgery, neurosurgery, plastic surgery, cardiothoracic surgery, general surgery, urology, otorhinolaryngology, ophthalmology, obstetrics and gynecology, and dental surgery. In addition, the biomaterials utilized encompass a wide range of sources (e.g., natural, synthetic, and hybrid polymers), format (e.g., scaffold, patch, spray, microneedle), and responsiveness (e.g., temperature, pH, and protein), which enables customized sEV therapy tailored to specific diseases. This review demonstrates biomaterial‐facilitated local delivery of stem cell‐derived sEVs as a viable alternative or even a superior option to systemic sEV therapy.

(139) What Penis Size and Glans-shape do Cis and Trans Men and Women Prefer?

Abstract Introduction The ideal length, circumference, and appearance of the penis are important to the self-confidence and body image of most men. To date, only 2 works have assessed preferred erect (but not flaccid) penis length, and only among cis-gender women. No study has evaluated preferred aesthetics--ideal erect and flaccid penile length, or, glans-shape and size preferences among cis-gender and transgender and non-binary individuals. These preference domains are especially important with genital gender-affirming surgery (gGAS) because length and glans-shape are entirely elective and technique-based, and help define neophallus appearance. Lastly, outlining the planned glans shape on a neophallus can be technically challenging, and, to date no publications have reported offering trans men input/choices. Objective 1) To query ideal for flaccid and erect penis length across all individuals, across cis- and transgender identities; 2) Determine specific likes/dislikes among all subjects for 7 different glans-shape examples; and 3) Among men undergoing phalloplasty, creation of glans-shape stencils for use in the OR, as a means to offer patients choice, and, to ensure that the final product aligns with patient’s choice. Methods An anonymous, online survey (QualtricsTM) evaluated glans preferences among 7 realistic prosthetic penis models shown. Participants were queried about their age, sex, gender, sexual orientation, and their ideal flaccid and erect penis length. Stencils were created based on 6 penis models. Subsequently, 7 transmen-subjects were able to choose the most-preferred glans shape and the stencil was applied before glansplasty surgery (Figure 1). Glansplasty results were assessed 12-weeks post-operatively (Figure 1c; E-F). Results 74 participants took the survey, (mean age of 35.3, Range: 23–59). 43/74 (58%) self-identified as men, and 28/74 (38%) identified as men, including 7 trans men (Table 1a). Men reported a mean ideal flaccid and erect length of 4.5 and 6.3-inches, respectively, while women reported a length of 5.2 inches and 6.16-inches, respectively. Women had a wider range of responses for both ideal flaccid and erect penile length (p>0.05) (Table 1b). For glans-shape preference, across all groups (independent of sex or sexuality), the “most-liked” options were A and E, and “most-disliked” were B and D (Figure 2). Among the 7 transmen undergoing glansplasty, 5/7 (71%) chose glans-shape C, and 1/7 (14%) each chose glans-shapes A and E, for their own phallus. Conclusions This is the first study to survey both men and women regarding ideal erect penile length, and the first regarding ideal flaccid penile length and glans-shape preferences. Our data suggests that the ideal erect and flaccid length are longer (within 2SD) than the mean values for cis-men of 5.16-inches and 3.61-inches respectively, (Miles et al., BJUI, 2014) but there is greater variability among women’s responses. Regarding glans-shape, most preferred a more tapered, bullet-like tip for the distal glans and a less prominent glans ridge. Finally, our glansplasty stencils are easy to use, yield excellent aesthetic results, and the option to choose their own glans shape is highly appreciated by patients. Disclosure No.