Radicular Dentin Research Articles

Effect of Final Irrigation Protocols on Radicular Dentin Permeability and Push-Out bond Strength in Endodontically Treated Teeth

This study aimed to analyze the effect of several final irrigation protocols on tubular permeability and push-out bond strength of an epoxy resin-based cement to radicular dentin. The crowns of 110 human teeth were removed to standardize at 17mm in length. The root canals were instrumented and irrigated with 5ml of 5% NaOCl and 17% EDTA. The samples were then randomly divided into 5 groups (n=22) according to the final irrigation protocol. Subsequently, the samples were subdivided into two different groups to assess dentin permeability or the evaluation of the push-out bond strength. In Group 1, all samples displayed the formation of brown-like precipitates. Group 2 and Group 5 demonstrated cleaner dentin walls. The push-out bond test after 72 hours denoted a significant difference when comparing Group 1 vs Group 2 and Group 1 vs Group 3. After thermocycling, a significant difference is observed between Group 1 and all remaining groups. The irrigation protocol using distilled water prior to irrigation with chlorhexidine significantly reduces the formation of intracanal precipitates by avoiding interactions between irrigants. The push-out bond strength of epoxy resin-based cement is directly influenced by the precipitates formed in the root canal after irrigation and the time of thermocycling.

Effect of resveratrol pretreatment of radicular dentin on immediate and delayed pushout bond strength of fiber post luted using self-adhesive resin cement: An in vitro study.

To assess and compare the effect of resveratrol pretreatment of root dentin on immediate and delayed pushout bond strength (POBS) of fiber postluted using self-adhesive resin cement. Postspace was prepared in endodontically treated roots and randomly divided into two groups based on pretreatment rinse before fiber postcementation: Group 1: Distilled water Group 2: 1 mL of 10 mg/mL resveratrol solution for 1 min. Fiber posts were luted using self-adhesive resin cement. Among, two 1 mm slices of the coronal and middle third of root, one slice was subjected to immediate POBS using the universal testing machine and the other slice was subjected to POBS after storing in distilled water for 12 months. Resveratrol pretreatment showed significantly higher immediate POBS with more mixed failure than distilled water group. There was no significant difference in delayed POBS between the distilled water (mean: 7.07 MPa) and resveratrol pretreated group (mean: 8.20 MPa) with predominantly adhesive failure among them. There was significant difference between immediate and delayed POBS value of both the groups. Resveratrol pretreatment can be considered to improve bond strength stability of radicular dentin to self-adhesive resin cement.

To compare the efficacy of various organic solvents on retrievability of Biodentine and their effect on microhardness of Biodentine and radicular dentin: An in vitro study.

Rotary and ultrasonic instruments are not appreciable in the complete removal of Biodentine from root canals. Therefore, organic solvents can be used as an adjunct for its retrieval. The aim of this study was to compare the efficacy of 10% citric acid, 2% acetic acid, and 20% tartaric acid on the microhardness of Biodentine and radicular dentin. Forty single-rooted extracted teeth were decoronated at the cementoenamel junction and root canals were prepared with peeso reamers. Two-millimeter sections were obtained and restored with Biodentine. All samples were subjected to Vicker's microhardness test to record the microhardness of Biodentine and radicular dentin. Samples were randomly divided into four experimental groups and treated with (n = 20) - distilled water, 10% citric acid, 2% acetic acid, and 20% tartaric acid groups for 10 min, after which specimens were again subjected to the same microhardness test. The data were subjected to Kruskal-Wallis ANOVA test, followed by Wilcoxon signed-rank test with a level of significance set at P ≤ 0.05. 10% citric acid followed by 2% acetic acid exhibited the lowest mean microhardness values after immersing in respective solutions, whereas 20% tartaric acid exhibited the highest mean microhardness values on Biodentine and radicular dentin. 10% citric acid can be used for retrieving Biodentine from root canals for a limited time without adversely affecting the physical and chemical composition of radicular dentin.

Flexural strength and microhardness of human radicular dentin sticks after conditioning with different endodontic chelating agents.

The objective of this in vitro study was to examine the impact of different endodontic chelating agents on the flexural strength and microhardness of root dentin. Fourty dentin sticks of (1 mm × 1 mm × 12 mm) were obtained from 10 single-rooted premolars and divided into four groups (n = 10). One stick from each tooth was assigned to one of the experimental groups and was soaked in one of the experimental chelating solutions for 5 min 17% ethylenediaminetetraacetic acid (EDTA), 2.5% phytic acid (PA), 18% etidronic acid, or saline (control group). Following the 5-min soak, the sticks' flexural strength was evaluated using a 3-point loading test using the universal testing machine, and the surface microhardness was tested using a Vickers's microhardness tester. PA (2.5%) and etidronic acid (18%) showed no significant detrimental effect on either the flexural strength or the surface microhardness of radicular dentin compared to the control. EDTA (17%) exhibited a significant drop in the flexural strength and microhardness of radicular dentin compared to the other groups. PA and etidronic acid chelators do not compromise the surface and bulk mechanical properties of radicular dentin.

Evaluation of Chemical Components Changes in Radicular Dentin After Different Final Surface Treatments

Evaluation of Chemical Components Changes in Radicular Dentin After Different Final Surface Treatments

Effect of Dentin Thickness on the Pulp of Single Rooted Teeth in Severe Chronic Periodontitis: Histological Study

Background The issues of dentine thickness on the influence of pulpal changes in teeth with deep periodontal pockets has not been assessed. So the present study evaluates the effect of dentin thickness on the pulp in severe chronic periodontitis.Objectivesi To assess the radicular dentin thickness histologically.ii To histologically assess the pulpal changes due to periodontitis.iii To correlate the effect of radicular dentin thickness and periodontitis on dental pulpMethods Single rooted periodontally compromised tooth was atraumatically extracted. Access opening was done using an extracted tooth and the pulp fixed in formalin solution. After sufficient fixation decalcification was done followed by processing and histological examination. The radicular dentin thickness was measured at 3 reference points and the mean value was compared with histological parameters to assess for any changes.Results Results revealed that various histological changes were observed in the pulp. The mean dentin thickness when compared with various histological parameters revealed varying results. Inflammation and edema did not corelate with changes in dentin thickness whereas fibrosis presence of pulp stone pulp vessels and odontoblastic integrity showed a positive correlation but were not statistically significant. Conclusion Within the limitations of the study although a statistically significant difference was not observed in the root dentin thickness and histological changes in the pulp there is a definitive increase in the severity of pulpal changes associated with inflammation.

Formation and Effect of Dentinal Microcracks After Root Canal Treatment

Many potential causes of root fracture have been put forth over the decades, such as theories that the fracture might begin with dentinal microcracks (DM) brought on by dentinal dehydration, post-insertion corrosion, spreader form, or strong movements utilized while filling processes. In the recent times, scientists have found that a sizable proportion of preexisting dentinal defects on the roots to be present. These problems are though too likely to be brought on by high extraction pressures and/or the way the teeth were stored. The limits of the standard methodologies are undoubtedly vulnerable to systematic analytical inaccuracies, making them far from an optimal experimental model, according to scientific rationale underlying this ambiguous scenario. It has also been shown that dentin moisture affects the biomechanical response of radicular dentin to root canal preparation, specimen storage circumstances are another issue with investigations on dentinal microcracks development. Instrumenting with manual, reciprocating, or rotary NiTi tools does not produce remnant microstrain accumulation in hydrated roots.

Does radiation therapy affect adhesion of tricalcium silicate cements to root dentin?

This study aimed to analyze the effect of irradiation on the push-out bond strength of mineral trioxide aggregate (MTA) and Biodentine to radicular dentin. A total of 60 extracted mature human teeth with single root canals were categorized into two groups (irradiated and non-irradiated) (n=30). Each group was further divided into two sub-groups based on cements used (Biodentine and MTA). Then, a cumulative radiation dose of 60 Gy was divided into 30 fractions (two Gy for every fraction) and administered for five successive days per week over six weeks. Obturation was then performed using MTA and Biodentine. Afterwards, 1.5 mm thick horizontal sections were procured from the middle one-third of all the specimens and then subjected to push-out bond test. Results were analyzed using one-way analysis of variance with post-hoc Tukey's test. The bond strength of Biodentine and MTA to irradiated teeth was lower than non-irradiated teeth. Highest push-out bond strength was observed in non-irradiated specimens filled with Biodentine (p=0), followed by irradiated specimens filled with Biodentine (p=0); non-irradiated specimens filled with MTA (p=0); and irradiated specimens filled with MTA (p=0.9). The push-out bond strength of Biodentine and MTA to root canal dentin decreased significantly post irradiation.

Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections.

This study aimed to evaluate the cytotoxicity and synergistic effect of epigallocatechin gallate (EGCG) and fosfomycin (FOSFO) on biofilms of oral bacteria associated with endodontic infections. This study determined minimum inhibitory and bactericidal concentration (MIC/MBC) and fractionated inhibitory concentration (FIC) of EGCG and FOSFO against Enterococcus faecalis, Actinomyces israelii, Streptococcus mutans, and Fusobacterium nucleatum. Monospecies and multispecies biofilms with those bacteria formed in polystyrene microplates and in radicular dentin blocks of bovine teeth were treated with the compounds and control chlorhexidine (CHX) and evaluated by bacterial counts and microscopy analysis. Toxicity effect of the compounds was determined on fibroblasts culture by methyl tetrazolium assays. The combination of EGCG + FOSFO demonstrated synergism against all bacterial species, with an FIC index ranging from 0.35 to 0.5. At the MIC/FIC concentrations, EGCG, FOSFO, and EGCG+FOSFO were not toxic to fibroblasts. EGCG+FOSFO significantly reduced monospecies biofilms of E. faecalis and A. israelli, whereas S. mutans and F. nucleatum biofilms were eliminated by all compounds. Scanning electron microscopy of multispecies biofilms treated with EGCG, EGCG+FOSFO, and CHX at 100x MIC showed evident biofilm disorganization and substantial reduction of extracellular matrix. Confocal microscopy observed a significant reduction of multispecies biofilms formed in dentin tubules with 84.85%, 78.49%, and 50.6% of dead cells for EGCG+FOSFO, EGCG, and CHX at 100x MIC, respectively. EGCG and fosfomycin showed a synergistic effect against biofilms of oral pathogens related to root canal infections without causing cytotoxicity.

Micro-hardness of the Radicular Dentine after Root Canal Irrigation with Salvadora Persica Extract: A laboratory Study

Micro-hardness of the Radicular Dentine after Root Canal Irrigation with Salvadora Persica Extract: A laboratory Study

Evaluation of dislocation resistance of three different resin-based root canal sealers from radicular dentin -An ‘in vitro’ study.


 Aim: The aim of this study is to evaluate the dislocation resistance of three different resin-based root canal sealers from radicular dentin -An ‘in vitro’ study.
 Methodology: Sixty extracted human maxillary canines were sectioned transversally below the cement-enamel junction to obtain 2-mm-thick dentin disks that were randomly divided into three groups (n = 20) for treatment with one of three different root-canal sealers (AH Plus [Dentsply DeTroy, Germany], Bioseal (Equinox Holland), EndoREZ [Ultra dent]). Standardized cavities were prepared to simulate root canals, cavities were filled with sealer material, and push-out bond-strength testing was performed using a universal testing machine.
 Result: In the present study amongst all the three sealers tested viz AH Plus, EndoREZ & Bioseal. EndoREZ (Group III) goes most superior (14.97 ± 3.17 MPa), followed by AH Plus (Group II) (13.29 ± 2.27 MPa) & the least bond strength was seen with Bioseal (Group I) (4.83 ± 1.62 MPa).
 There was a gradual decrease in bond strength from a coronal third of radicular dentin to the middle third followed by an apical third in all three groups.
 Conclusion: Amongst all the three-sealer tested EndoREZ® gave the maximum bond strength, followed by AH Plus® & then Bioseal. Bioseal gave the least bond strength irrespective of the area in radicular dentin. There was a gradual decrease in bond strength from the coronal third of radicular dentin to the middle third followed by an apical third in all three groups.

Farklı Işığa Duyarlılaştırıcılar ile Uygulanan Foto Dinamik Terapinin Konvansiyonel İrrigasyon ile Karşılaştırılarak Dentin Sertliğine Etkisi

Objective: The aim of this study was to examine the effect of photodynamic therapy (PDT) using toluidine blue O (TBO), curcumin (CUR) and methylene blue (MB) photosensitizers on root canal dentin microhardness by comparing it with sodium hypochlorite (NaOCl) + ethylenediamine tetraacetic acid (EDTA).
 Materials and methods: The root canals of 100 human premolar teeth were shaped by the R25 file (Reciproc; VDW, Munich, Germany). The working length of the teeth was determined by using a #10 K-file, keeping it 1mm shorter than the tooth apex. The R25 file was used to prepare the root canals. After every three pecking motions, irrigation was performed and a total of 10 mm of distilled water was used. The specimens were randomly distributed according to the disinfection method; NaOCl+EDTA, PDT with TBO, PDT with CUR, PDT with MB and distilled water (DS) (n=20). Grooves were prepared on the buccal and lingual surfaces of the prepared teeth, parallel to the long axis of the tooth, without touching the root canals. The roots were divided into two parts by means of a cement spatula placed in these grooves. Root canal dentin microhardness was evaluated by the Vickers test method. Three measurements were made by applying 300 g of force for 15 seconds and the average was calculated. It was recorded as the Vickers hardness value. The data were analyzed by using the one-way ANOVA and Dunnett’s post hoc tests (α=0.05).
 Results: All photosensitizer groups showed significantly higher microhardness value than the groups of DS and NaOCl + EDTA (p0.05). 
 Conclusions: PDT with CUR obtained the highest radicular dentin microhardness.

Evaluation of time exposure of different irrigation agents on radicular dentin erosion

Aim: The amount of root dentin erosion caused by Ethylene diamine tetraacetic acid (EDTA), Glycolic acid (GA), and Etidronic acid (HEDP) solutions applied with sonic activation (SA) for 1 and 3 minutes during the final irrigation was evaluated in this in vitro study. Methods: Using 5 ml of 2.5% NaOCl irrigation solution in each sample, 70 mandibular premolars were enlarged with the Reciproc R25 rotary instrument system. The samples were randomly divided according to the final irrigation protocol into 6 experimental groups (n:10) and 1 control group (n:10): Group 1 (17% EDTA – 1 min SA), Group 2 (17% EDTA – 3 min SA), Group 3 (10% GA – 1 min SA), Group 4 (10% GA – 3 min SA), Group 5 (18% HEDP – 1 min SA), Group 1 (18% HEDP – 3 min SA) and Group 7 (Negative control group-distilled water, 1 min SA). The teeth were divided into two parts longitudinally after the final irrigation protocol. A low vacuum scanning electron microscope (SEM) was used to image the samples’ coronal, middle, and apical root areas at x2000 magnification. Samples were evaluated using a triple-scoring system. Results: Data were analyzed by Shapiro Wilk, Kruskal Wallis H, Mann-Whitney U, and Dunn’s tests. There was no statistically significant difference between EDTA, GA, and HEDP in both periods (p > 0.05). Conclusion: We saw similar amounts of erosion in dentin in 1 and 3 minutes of chelation applications for all chelators. Considering the smear removal efficiency of chelators, there is no harm in increasing the time in terms of erosion. There is a need for studies on the clinical applicability of chelation with a different duration time. How to cite this article: Okumuş ÖF, Kaya S, Adıgüzel Ö, Yeniçeri Özata M. Evaluation of time exposure of different irrigation agents on radicular dentin erosion. J Med Dent Invest 2022;3:e221170. https://doi.org/10.5577/jomdi.e221170 Linguistic revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English. Checked for plagiarism: Yes

Effect of three different photosensitizers in photodynamic therapy on bond strength of a calcium silicate-based sealer to radicular dentin.

To evaluate the effect of the photodynamic therapy (PDT) using with different photosensitizers on the bond strength of BioRoot RCS (BR) to radicular dentin by comparing with AH Plus (AH). The shaped root canals of 80 human mandibular premolars were divided into four groups based on the final disinfection protocol: NaOCl/EDTA, PDT with methylene Blue (MB), PDT with curcumin (CUR) and PDT with toluidine blue O (TBO) (n=20). Each group was randomly divided into two subgroups (n=10), according to the endodontic sealer used for obturation (n=10). The test of push-out bond strength (PBS) was performed using an universal test machine. The data were analysed using ANOVA, Tukey post-hoc, and independent sample t-test (α=0.05). MB-BR group showed the highest PBS at the cervical level (p < 0.05). The selection of the photosensitizer in PDT influenced adhesion of root canal sealers to radicular dentin.

Effect of Nano Forms of Propolis and Antibiotic Pastes as canal Medicaments on Radicular Dentin Microhardness. (In-Vitro Study)

Effect of Nano Forms of Propolis and Antibiotic Pastes as canal Medicaments on Radicular Dentin Microhardness. (In-Vitro Study)

Push-Out Bond Strength Failure Analysis of Gutta-Percha and Martens Hardness of Radicular Dentin Disinfected Using Rose Bengal, Er, Cr: YSGG, Sealed with a Novel Bio-Ceramic Sealer

Push-out bond strength (POBS) and failure analysis of gutta-percha and Martens hardness (MH) of canal dentin sealed with Bio-ceramic sealer (BCS). Specimens underwent endodontic treatment. Based on the method of disinfectants, specimens were classified into 4 groups. Group 1:5.25% NaOCl+Q-mix, group 2:ECYL+Q-mix, group 3:LGE+Q-mix, and group 4:RBP+Q-mix. Before obturation MH was assessed using a microhardness tester. Canals were obturated, and sealed with Bio-Root RCS. Each root was cut horizontally in 2 mm thickness and placed under the indenter of the universal testing machine. One-way analysis of variance and the Post Hoc Tukey test for group comparisons as statistical analysis. Samples with (5.25% NaOCl+Q-mix) displayed the highest bond integrity of BCS (8.00±0.24 MPa). The lowest POBS was demonstrated by the apical section of group 4 (RBP+Q-mix 2 in 1) (4.10±0.94 MPa) specimens. Group 2 (ECYL+Q-mix) and group 3 (LGE+Q-mix) exhibited comparable outcomes of bond scores to group 1 specimens (p &gt; 0.05). MH was significantly lower in group 4 compared to group 1, group 2, and group 3 (p &lt;0.05). The bond strength of BCS and MH of canal dentin is influenced by the type of irrigation.

Effect of Endodontic Irrigating Solutions on Radicular Dentine Structure and Matrix Metalloproteinases-A Comprehensive Review.

Irrigating solutions play an important role in the eradication of intracanal microbes and debris dissolution during endodontic treatment. Different combinations of solutions and protocols have been advocated, with sodium hypochlorite (NaOCl), ethylenediamine tetra acetic acid (EDTA), and chlorhexidine (CHX) remaining the most widely used ones by many clinicians. Although these solutions provide efficient inorganic dissolution and antimicrobial capacity, their use has also been reported to cause undesired effects on root dentin composition and mechanical and biomechanical properties, such as microhardness, surface roughness, bond strength, and matrix metalloproteinase (MMP) activity. Several corroborating studies attribute these changes in mechanical properties of dentine to the use of irrigating solutions, and there are limited reports on how the solutions affect the expression of MMPs, which may be a correlating link to understanding the role of these enzymes in dentin collagen and changes in the mechanical properties of dentin. Hence, using the basis of several studies from the literature, the objective is to comprehensively review the influence of individual and combined irrigating solutions on root dentine structure and the activity of the MMPs.

The effectiveness of EDTA 17% as a cleaning solution for the fiber post space after filling with cements

Aim: To evaluate the resistance of the union between a glass fiber post and radicular dentine after cleaning the root with 17% EDTA and filling with different endodontic cements. Methods: Forty uniradicular bovine incisors were removed to obtain root lengths of 18 mm. Endodontic treatment was performed on all roots using different filling cements (zinc oxide and eugenol-based, OZE; cement based on epoxy resin, AH) and cleaning solutions (saline, SA or EDTA), which made it possible to obtain four groups: OZESA, OZEEDTA, AHSA and AHEDTA. Subsequently, 12 mm of filling material was removed from the roots, and they were prepared to receive fiber posts luted with resin cement. To execute the mechanical cycles (2x106 cycles, 90 N, 4 Hz), coronal reconstruction was performed with a silicon matrix. The roots were then sliced (2-mm thick) to perform the push-out test. The results were analyzed using analysis of variance (one factor and two factors) and Tukey’s test (α=0,05). Results: Bond strength (Mpa) was significantly higher for OZEEDTA (9,18) and AHEDTA (8,70) than for OZESA (6,06) AHSA (8,7). OZEEDTA also presented the highest values in the cervical region (15,18) but was significantly lower in the apical region (2,99). However, AHEDTA had a homogeneous bond strength in all thirds. Conclusion: Regardless of the endodontic cement used, EDTA was used as an irrigating solution, culminating in a higher bond strength between the glass fiber post and dentin.

Use of photosensitizers activated by photodynamic therapy on the canal disinfection of radicular dentin bonded to Dimethacrylate-based glass fiber post: an assessment of pushout bond strength.

The present lab-based investigation aimed at evaluating the canal disinfectants using photodynamic therapy (PDT) using different photosensitizers, conventional NaOCl, a mixture of doxycycline, citric acid, and a detergent (MTAD) and their impact on the bond strength of glass fiber post to radicular dentin. Forty extracted human premolars were gathered and disinfected. The decoration was performed up to the cementoenamel junction. Using the crown down technique cleaning of the canal was done following rinse with distilled water. All canals were dried with paper points and obturated with gutta-percha. Post space was prepared using peso reamers and samples were randomly allocated into four groups following different disinfection regimes: Group 1 - Methylene blue photosensitizer (MBP) + MTAD; Group 2 - RBP (Rose Bengal photosensitizer) + MTAD; Group 3 - CP (curcumin photosensitizer) + MTAD and Group 4 - 2.25% NaOCl + MTAD (control). Following disinfection, the canals were dried and the post was placed and cemented within the canal. Samples were dissected at coronal, middle, and apical third and placed in a universal testing machine for push-out bond strength (PBS). Debonded surfaces were evaluated for failure modes. PBS was examined using a one-way analysis of variance (ANOVA). The means of PBS were compared using Tukey multiple comparison tests with a significance threshold of (p<0.05). Group 3 canals disinfected with CP and MTAD had the highest PBS at two levels: cervical (9.57±1.21 MPa), middle (6.37±0.79 MPa), and group 2 canal space disinfected by RBP and MTAD had the maximum PBS in apical portion (5.35±0.42 MPa). No significant difference at all root levels between group 2 (RBP + MTAD) and group 3 (p>0.05). Canal irrigation with group 1 (MBP + MTAD) and group 4 control (2.25% NaOCl + MTAD) exhibited comparable PBS at all three levels of the root. CP, MTAD, RBP and MTAD for canal disinfection and bonding of glass fiber post to radicular dentin demonstrated comparable bond values at all three root levels and can be recommended in clinical settings after further investigations.

The early wear susceptibility of cementum, enamel and dentine in vitro

ObjectivesTo assess the differential early wear susceptibility of cementum, enamel and dentine at a micron level. MethodsWhole human molar buccal surfaces incorporating natural enamel and cementum (n = 20) confirmed by imaging (digital microscopy: Keyence, VHX-7000 Milton Keynes, UK), were mounted, scanned (profilometry: XYRIS 4000, Taicaan, Southampton, UK), and allocated to receive erosion (citric acid, pH 2.7, 30 min (n = 10)) or erosion/abrasion challenges (3 cycles of (citric acid, pH 2.7, 10 min, 60 300 g linear abrasion strokes), n=10). Samples were polished and the experiment repeated on polished enamel, and polished coronal and radicular dentine within the same tooth. Profilometric wear data were obtained using superimposition: GeoMagic (3Dsystems, Darmstadt, Germany) and subtraction: MountainsMap (DigitalSurf, Besancon, France). Data were normal. A general linear model was used to assess differences between groups and substrates. ResultsThe mean step height (SD) for natural enamel was 8.82 μm (2.53) for erosion and 11.48 μm (2.95) for erosion/abrasion. For natural cementum, the mean step height was 6.00 μm (2.29) for erosion and 4.67 μm (1.58) for erosion/abrasion. Dentine step heights ranged from 7.20 μm (1.53) for erosion and 9.79 μm (1.01) for erosion/abrasion with no statistical differences in dentine wear. Natural cementum surfaces had the lowest wear (p<0.001). Dentine had significantly less wear than natural enamel (p<0.02). ConclusionsCementum surfaces demonstrated the most wear resistance, followed by dentine under erosion dominant conditions in this in vitro study. Further in-vivo investigations are needed to confirm the intraoral stability of cementum. Clinical SignificanceCementum may be the least susceptible of dental substrates to wear and dentine does not wear at a faster rate than enamel under erosive conditions. This adds to our knowledge on the development of non-carious cervical lesions and questions whether wear rates will accelerate once dentine is exposed.