Radicular Dentin Research Articles

Photodynamic therapy on the extrusion bond strength of gutta-percha to radicular dentin sealed with bioceramic and conventional sealers.

The aim of this study was to evaluate the influence of photodynamic therapy (PDT) on the extrusion bond strength (EBS) of gutta-percha to radicular dentin sealed with bioceramic sealers. Sixty human-extracted mandibular premolars were used in the present study, being decoronated up to the cementoenamel junction, and secured in heat cure acrylic resin for root canal therapy. The specimens were randomly divided into groups based on conventional [2.25% sodium hypochlorite (NaOCl) + 17% EDTA] and disinfection protocols (2.25% NaOCl + PDT + 17% EDTA) (n = 10). Specimens in groups 1, 3, 5: conventional treatment modality (2.25% NaOCl + 17% EDTA). Samples in groups 2, 4, 6: adjunctive PDT treatment modality (2.25% NaOCl+ PDT+ 17% EDTA). Specimens in groups 1 and 2 were sealed with AH Plus sealer (AH). Specimens in groups 3 and 4 were sealed using Endo Sequence BC sealer, and samples in groups 5 and 6 were sealed with MTA Fillapex. All specimens were cut in the coronal and middle segments and positioned in a universal testing machine (UTM) for assessment of extrusion bond strength (EBS). ANOVA and Post-Hoc Tukey multiple comparison tests were applied for performing statistical analysis (p < 0.05). Coronal root samples in group 1 (2.25% NaOCl + 17% EDTA) sealed with AH Plus sealer demonstrated the highest EBS value (9.21 ± 0.62 MPa) whereas the middle-third of specimens in group 6 (2.25% NaOCl + PDT + 17% EDTA) sealed with MTA Fillapex exhibited the lowest EBS value (5.07 ± 0.17 MPa). Intergroup comparison revealed that group 3 (2.25% NaOCl + 17% EDTA) sealed with Endo Sequence BC Sealer and group 5 (2.25% NaOCl + 17% EDTA) sealed with MTA Fillapex disclosed comparable EBS results to group 1 (p > 0.05) while group 2 (2.25% NaOCl + PDT + 17% EDTA) sealed with AH Plus sealer and group 4 (2.25% NaOCl + PDT + 17% EDTA) sealed with Endo Sequence BC Sealer unveiled analogous EBS values to group 6 (2.25% NaOCl + PDT + 17% EDTA) MTA Fillapex (p > 0.05). The most noticeable failure mode in the coronal and middle thirds of non-PDT subjected groups was cohesive. Combination of 2.25% NaOCl with PDT and 17% EDTA for canal disinfection with AH Plus sealer, calcium silicate sealer, and MTA-based bioceramic sealers have an unfavorable effect on the EBS of gutta-percha to the root canal wall.

Effect of Heated Sodium Hypochlorite Irrigant on Structural Changes and Microhardness of Radicular Dentin: An In Vitro Study.

This study is aimed to evaluate the combined effect of sodium hypochlorite at varied concentrations and temperatures on radicular dentin microhardness along with its surface structural changes using an FTIR spectrometer. Mandibular premolars were cleaned and shaped up to F3 Protaper gold rotary files, after which they were subjected to five experimental conditions - group I - neutral saline as negative control, group II - 3% NaOCl solution, group III - 5% NaOCl solution, group IV - 3% intracanal-heated NaOCl solution, and group V - 5% intracanal-heated NaOCl solution. Following this, the microhardness of radicular dentin at 100 µm and 300 µm from the canal lumen and Fourier-transform infrared (FTIR) spectroscopic analysis were performed. The results showed that intracanal-heated sodium hypochlorite group reduced root dentin microhardness at 300 µm than its nonheated counterpart. No difference in microhardness values was observed between 3% intracanal-heated and room-temperature sodium hypochlorite groups at 100 µm. Reduction in amide/phosphate ratio was noted in all the groups treated with sodium hypochlorite irrespective of temperature and concentration. Thus, considering that the level of alteration in physical and structural changes of root dentin with or without heating is insignificant, intracanal-heated low-concentration sodium hypochlorite solutions could be used as an alternative to high-concentration sodium hypochlorite. Intracanal-heated low-concentration sodium hypochlorite enables the clinicians to achieve maximum disinfection while keeping the structural and physical properties of the dentin similar to room-temperature sodium hypochlorite.

Biological effect of a novel alcoholic and aqueous plant extracts of moringa as a bioactive irrigant on radicular dentin ultra-structural surface morphology

Biological effect of a novel alcoholic and aqueous plant extracts of moringa as a bioactive irrigant on radicular dentin ultra-structural surface morphology

Assessment of the Orthodontic External Resorption in Periodontal Breakdown-A Finite Elements Analysis (Part I).

This Finite Elements Analysis (FEA) assessed the accuracy of Tresca failure criteria (maximum shear stress) for the study of external root resorption. Additionally, the tooth absorption-dissipation ability was assessed. Overall, 81 models of the second mandibular premolar, out of a total of 324 simulations, were involved. Five orthodontic movements (intrusion, extrusion, rotation, translation, and tipping) were simulated under 0.6 N and 1.2 N in a horizontal progressive periodontal breakdown simulation of 0-8 mm. In all simulations, Tresca criteria accurately displayed the localized areas of maximum stress prone to external resorption risks, seeming to be adequate for the study of the resorptive process. The localized areas were better displayed in the radicular dentine-cementum component than in the entire tooth structure. The rotation and translation seem prone to a higher risk of external root resorption after 4 mm of loss. The resorptive risks seem to increase along with the progression of periodontal breakdown if the same amount of applied force is guarded. The localized resorption-prone areas follow the progression of bone loss. The two light forces displayed similar extensions of maximum stress areas. The stress displayed in the coronal dentine decreases along with the progression of bone loss. The absorption-dissipation ability of the tooth is about 87.99-97.99% of the stress.

Effect of Different Protective Bases on pH Changes and Hydrogen Peroxide Microleakage During Intracoronal Bleaching.

Objectives: This study aimed to evaluate the pH changes and penetration of hydrogen peroxide into radicular dentin when different protective bases were used with and without a bonding agent. Materials and Methods: In this in-vitro experimental study, 70 single-rooted bovine teeth were instrumented and obturated with gutta-percha. The gutta-percha was removed 3mm below the cementoenamel junction (CEJ) and the teeth were divided into seven groups (n=10). In each group, 2mm base (1mm apical to the CEJ) was applied as follows: TheraCal LC, TheraCal LC plus SE Bond, Lime-Lite, Lime-Lite plus SE Bond, Ionoseal, Ionoseal plus SE Bond, and resin-modified glass ionomer (RMGI). The teeth were placed in vials containing distilled water, and pH values and molar concentration of the medium surrounding the teeth were recorded immediately after internal bleaching with 35% hydrogen peroxide. The pH values were also recorded at 1, 7, and 14 days following renewal of the medium. Data were analyzed with t-test, one-way ANOVA, and Kruskal-Wallis test. Results: After bleaching, the medium pH became acidic in all groups. There were no significant differences among groups in the mean pH of the medium after bleaching (P=0.189). Moreover, there were no significant differences among the study groups with respect to hydrogen peroxide concentration (P=0.895). Conclusion: Intra-orifice barriers such as light-cure resin-modified calcium hydroxide, light-cure resin-reinforced glass ionomer, and light-cure calcium silicate can be as effective as RMGI in providing coronal seal during intracoronal bleaching.

Use of final irrigants MTAD, Salvedora Perscia, Malachite green, and Ti-sapphire laser on push-out bond strength of Zirconia post

AIM: Evaluation of the push-out bond strength (PBS) of zirconia post-bonded to radicular dentin after using different final irrigants (MTAD, Malachite green (MG), Ti-sapphire laser and Salvadora persica (S.persica). MATERIAL AND METHOD: Forty human permanent single-rooted were decoronated above the cement-enamel junction. An experienced endodontist performed all the root canal instrumentation using ProTaper universal rotary files. Canals were irrigated using 5.25% NaOCl solution followed by EDTA as a final sterilant. Obturation with gutta-percha using AH Plus sealer was performed. Post-space preparation was completed using Gates Glidden and specimens were randomly assigned to four groups based on the final disinfectant received (n=10). Group 1: 5.25% NaOCl + MTAD, group 2: 5.25% NaOCl + MG, group 3: 5.25% NaOCl +Ti-sapphire laser, and group 4: 5.25% NaOCl + S. persica. The chemically polymerized resin was used to lute zirconia posts. PBS and failure mode analysis were performed using a universal testing machine and stereomicroscope at 40X magnification. Data were compared between the two groups with 95% CI using one-way analysis of variance (ANOVA) with the Tukey post hoc test. p=0.05. RESULTS: Group4(5.25% NaOCl+ S.persica) specimens demonstrated the maximum (8.94±0.14 MPa) bond strength. Conversely, the apical third of Group 2 (5.25% NaOCl+ MG) (2.87±0.15 MPa) samples revealed the minimum bond strength scores. Intergroup comparison exposed that Group 1 (1.3% NaOCl+ MTAD), Group 3 (5.25% NaOCl+Ti-sapphire laser), and Group 4 (5.25% NaOCl+ S. persica) at all three-thirds unveiled no significant difference in PBS (p<0.05). CONCLUSION: Ti-sapphire laser and Salvedora Persica possess the potential to be used as a final root canal irrigant to improve the push-out bond strength of zirconia post-to-root dentin.

Evaluation of Er,Cr:YSGG laser technique for fiber post removal of endodontically treated teeth using micro-computed tomography.

Various clinical techniques such as removal kits, ultrasonic tips, burs, and drills, have been used for fiber post removal in endodontically treated teeth. In most clinical cases, the dental practitioners prefer to use ultrasonic tips, despite the heat generation and the formation of microcracks induced in the radicular dentin. The purpose of this study was to investigate the effectiveness of using erbium, chromium: yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser (2780 nm) as an alternative fiber post removal technique and to compare it to an ultrasonic method using micro-computed tomography (micro-CT). The operating parameters of the X-ray tube were set to 50 kVp and 300 mA. This approach allowed the generation of 2D lateral projections that were then used to reconstruct the 3D volume in DICOM format. Fiber posts were removed from 20 endodontically treated single-rooted premolars (n = 10) using an ultrasonic vibrator with diamond-coated ultrasonic tip (control method), or Er,Cr:YSGG laser irradiation protocol; average power 2.5 W, repetition rate 20 Hz, pulse duration 140 μs, 40% air and 20% water, and close-contact mode. The number of sections with newly formed microcracks, the loss of dentinal tissue, the amount of the residual resin cement, and the removal time were evaluated for both methods. The data were analyzed using paired t-test, Wilcoxon signed-rank and Mann-Whitney U tests at level of significance a = .05. In the laser-treated group the parameters regarding microcracks formation (21 ± 16) and removal time (4.7 ± 1.1 min) were advantageous compared to the ultrasonic-treated group (42 ± 27 and 9.2 ± 1.0 min, respectively), indicating that Er,Cr:YSGG laser could be an alternative fiber post removal technique.

Push out bond strength of fiber post to radicular dentin using Q-mix, lemon/garlic extract, and riboflavin activated by photodynamic therapy as a final canal irrigant.

The aim of this study was to assess the extrusion bond values of fiber post to radicular dentin when disinfected using different final irrigants lemon garlic extract (LGE), riboflavin (RFP) activated by PDT (photodynamic therapy), and Q-mix 2-in-1. Forty single-rooted mandibular premolar teeth were decoronated. Endodontic treatment was performed, and the canals were continually irrigated with normal saline, dried with paper points, and obturated. Post space was prepared by removing gutta-percha using peso-reamers. All specimens were randomly allocated into four groups based on the final irrigant used. Specimens in Group 1 irrigated with: 5.25% NaOCl+17% EDTA, group 2: 5.25% NaOCl+Q-mix 2-in-1, group 3: 5.25% NaOCl+RFP, and group 4: 5.25% NaOCl+LGE. Following final irrigation, a fiber post was placed in the canal space and luted. Samples were sectioned and each section was placed in a universal testing machine to assess bond values. Debonded samples were assessed for failure modes, EBS and modes of failure. For comparisons among groups, the one-way analysis of variance (ANOVA) test and the Post-Hoc Tukey HSD test were used keeping the level of significance at p=0.05. The cervical section of samples in group 2 (NaOCL+Qmix) (7.11±0.81 MPa) exhibited the maximum value of EBS. However, the apical section of samples in group 3 (5.25% NaOCl+RFP) (3.33±0.26 MPa) displayed minimum extrusion bond values. Group 3 specimens in which final irrigation was performed with RFP established significantly lower bond integrity values than all the other investigated groups coronal (3.77±0.13 MPa), middle (3.60±0.41 MPa), and apical (3.33±0.26 MPa) (p<0.05). Intragroup comparison analysis, the coronal and middle root sections of all the experimental groups displayed comparable outcomes of EBS (p>0.05). However, near the apical section of the root, the bond strength of all the groups declined considerably. Q-mix 2-in-1 as the final irrigant displayed the highest extrusion bond strength of fiber-reinforced composite to canal dentin at all three levels coronal, middle and apical. Lemon garlic extract has the potential to be used alternative to ethylene diamine tetra acetic acid as a final irrigant.

Effect of MMP Inhibitors on the Bond Strength of Fibre Posts After Ageing

AimThis in vitro study aimed to evaluate the effect of matrix metalloproteinase (MMP) inhibitors on the bond strength of resin-cemented fibre posts to radicular dentin under an aged-loaded condition. Materials and methodsRadicular dentin was prepared and irrigated by MMP inhibitor solution after root canal obturation in 60 extracted single-rooted teeth based on 6 groups: (1) 2% chlorhexidine (CHX) + loaded; (2) CHX + unloaded; (3) 0.5% benzalkonium chloride (BAC) + loaded; (4) BAC + unloaded; (5) 17% ethylenediaminetetraacetic acid (EDTA) + loaded; and (6) EDTA + unloaded. After final rinsing, all specimens were sliced cross-sectionally and kept in a water bath for 12 months of ageing. Groups 1, 3, and 5 were subjected to cyclic loading. Push-out tests were conducted using a universal testing machine, and failure mode was examined. The data were analysed using 3-way analysis of variance and post hoc tests at α = 0.05. ResultsBAC + unloaded demonstrated the highest mean bond strength (3.12 ± 0.18 MPa; P < .001), while the BAC + loaded and CHX + loaded groups showed a significantly lower push-out bond strength than their unloaded counterparts. Mixed adhesive-cohesive failure was the most common failure mode observed. ConclusionsWithout cycling loading, BAC was superior to CHX and EDTA in preserving the bond strength of resin-cemented fibre posts after 12 months of ageing. Loading significantly weakened the effectiveness of BAC and CHX in preserving the bond strength.

Evaluation of interfacial adaptation and penetration of bioceramic-based sealers in oval root canals: A confocal laser scanning microscope study.

To assess the interfacial adaptation and penetration depth of three different bioceramic-based sealers (CeraSeal, EndoSeal MTA, Nishika Canal Sealer BG) compared to an epoxy resin-based sealer (AH Plus) in oval root canals. Fourty extracted single-rooted mandibular premolar with oval canal were prepared and randomly allocated according to the obturation into; CeraSeal, EndoSeal MTA, Nishika Canal Sealer BG and AH Plus. The roots were sectioned at 3, 6 and 9 mm from the apex. The sealer adaptation and the penetration depth were evaluated under confocal laser scanning microscope. One-way ANOVA and Repeated measure ANOVA were used to statistically analyze the data. Nishika Canal Sealer BG showed significantly higher sealer adaptation than EndoSeal MTA (P < .001) at apical and middle thirds. Meanwhile, AH Plus showed significantly higher sealer adaptation than EndoSeal MTA (P = 0.011) at middle third. For sealer penetration, Nishika Canal Sealer BG showed the longest sealer penetration that was significant compared to AH Plus (P < .001) and EndoSeal MTA (P < .001) whereas CeraSeal was significantly higher than EndoSeal MTA (P = 0.029) at coronal third. For AH Plus, there was a significant less sealer penetration at coronal third compared to apical and middle thirds (P < .05). Whereas for EndoSeal MTA, the coronal third has significant less penetration compared to the middle third (P = 0.032). Endoseal has the lowest adaptation and penetration depth. Nishika Canal Sealer BG has better adaptation and penetration depth using single cone obturation technique in oval canal. RESEARCH HIGHLIGHTS: All the tested root canal sealers have some percentage of gaps and vary in their penetration capability into dentinal tubules. Nishika Canal Sealer BG has significantly better sealer adaptation to root dentinal walls than that of EndoSeal MTA at the apical and middle third but not significantly different from other type of sealers used. Nishika Canal Sealer BG has significantly better penetration depth than AH Plus and EndoSeal MTA at the coronal third of radicular dentin.

Progress in the classification of hereditary dentin disorders and clinical management strategies

Heterogeneous mutations in dentin sialophosphoprotein (DSPP) gene, which is located on autosome 4, are associated with hereditary dentin developmental disorders. According to the new classification proposed by de La Dure-Molla et al, diseases caused by DSPP gene mutations mainly manifested as abnormal dentin development are collectively referred to as dentinogenesis imperfecta (DI), including dentin dysplasia type Ⅱ (DD-Ⅱ), dentinogenesis imperfecta type Ⅱ (DGI-Ⅱ) and dentinogenesis imperfecta type Ⅲ (DGI-Ⅲ) in Shields classification. And dentin dysplasia type Ⅰ (DD-Ⅰ) in Shields classification is redesignated as radicular dentin dysplasia. In this paper, progress in the classification, clinical characteristics and genetic mechanisms of DI are reviewed. This paper also provides clinical management and treatment strategies for patients suffering DI.

Effectiveness of final cavity disinfectant terminalia chebula, malachite, and indocyanine green, against E. Faecalis and on the bond interface of fiber post to radicular dentin

AimsEvaluation of antimicrobial efficacy of contemporary disinfection Malachite green (MG), Terminalia chebula (T. chebula), and Indocyanine green (ICG)) against E. faecalis and their effect on push-out bond strength (PBS) of glass fiber post (GFP) bonded to root dentin in comparison to conventional irrigation used (Sodium hypochlorite (NaOCl) + Ethylene diamine tetraacetic acid (17% EDTA). Materials and methodsRoot canal treatment was initiated on sixty human single-rooted premolars and working length was established. The canals were enlarged using ProTaper universal system till F3. The prepared canals were obturated with Gutta-percha (GP) and AH Plus sealer followed by post-space preparation. Twenty canals contained a standard strain of E. faecalis (n=5 in each group) cultured overnight at 37 °C in BHI broth. All the samples were randomly allocated into four groups based on post-space irrigation. (n = 10) Group 1: 5.25% NaOCl+17% EDTA (Control), Group 2: 5.25% NaOCl + ICG, Group 3: 5.25% NaOCl + MG and Group 4: 5.25% NaOCl + T. chebula. Survival rates of E. faecalis were evaluated on the twenty specimens. GFP was placed on the remaining samples using self-etch dual-cure pastes and sectioning was performed in 1 mm thick slices. PBS and failure mode were analyzed using a universal testing machine and stereomicroscope. One-way analysis of variance (ANOVA) and Tukey multiple comparison t-tests were used for data analysis. (p = 0.05). Results5.25% NaOCl+17% EDTA demonstrated (0.11±0.01 CFU/mL) the lowest survival rate of E. faecalis. However, Group 2 (5.25% NaOCl + ICG) exhibited the highest survival rate (0.52 ± 0.10). Furthermore, the coronal third of group 4 (5.25% NaOCl + T. chebula) (8.71±0.25 MPa) specimens demonstrated the highest PBS of GFP. However, an apical section of group 2 samples (5.25% NaOCl + ICG) (1.21±0.71 MPa) displayed the lowest outcome of bond integrity. ConclusionTerminalia chebula when used as a final canal disinfectant seems to be promising in improving canal sterility and the bond strength of GFP to the root dentin.

Effectiveness of Morinda citrofolia, Sapindus mukorossi, and curcumin used in curry spice, to the radicular canal irrigant in improving bond integrity of glass fiber post

AimEvaluation of the push-out bond strength (PBS) of glass fiber reinforced post (GFRP) bonded to root dentin after canal disinfection using food-based root canal irrigants i.e., Curcumin photosensitizer (CP), Riboflavin photosensitizer (RFP), Morinda ctrifolia juice (MCJ) and Sapindus mukorossi (SM) along with MTAD as a final irrigant. Material methodsFifty human single-rooted premolar teeth were decoronated. Endodontic preparation was performed along with 2.25% sodium hypochlorite NaOCl solution followed by EDTA solution. Canals were dried and obturated followed by post-space preparation by removing GP. Specimens were allocated into five groups based on different food-based disinfection regimes (n = 10). Group 1: 2.25% NaOCl + MTAD (Control), Group 2: 6% MCJ + MTAD, Group 3: SM + MTAD, Group 4: CP + MTAD and Group 5: RFP + MTAD. All GFRP were bonded to radicular dentin. Root sectioning was performed followed by PBS and failure analysis using a universal testing machine (UTM) and stereomicroscope respectively. The data were analyzed using a one-way analysis of variance (ANOVA) test and the Post Hoc Tukey HSD test (p = 0.05). ResultsSamples disinfected with(MCJ+MTAD) at coronal third demonstrated maximum PBS (9.41 ± 0.51 MPa). However, the apical third of group 5 (RFP + MTAD) exhibited the minimum values (4.06 ± 0.23 MPa). Intergroup comparison analysis unveiled that group 2 (MCJ +MTAD) and group 3 (SM+MTAD) displayed comparable outcomes of PBS at all three-thirds. Similarly, samples in group 1 (2.25% NaOCl + MTAD), group 4 (CP + MTAD), and group 5 (RFP + MTAD) exhibited comparable PBS. ConclusionFruit-based irrigants Morinda citrifolia and Sapindus mukorossi have the potential to be used as root canal irrigants with a positive influence on bond strength.

Adjunctive use of Er, Cr: YSGG laser, Potassium titanyl Phosphate laser, and photosensitizer on extrusion bond strength of radicular dentin to bioceramic sealers.

To assess the impact of adjunctive final root canal disinfection regime curcumin photosensitizer (CP) activated by Photodynamic therapy (PDT), Er, Cr: YSGG laser (ECL), and Potassium titanyl Phosphate (KTP) laser on the bond integrity of two endodontic Bioceramic (BC) sealers. Decoration of eighty human single-rooted teeth was performed up to a cementoenamel junction. After instrumentation, all samples were irrigated with 2.25% NaOCl solution followed by 17% EDTA. The specimens were divided arbitrarily into 8 groups (n=10) based on the adjunctive final irrigation method used. Group 1 and 5 (Control): No adjunctive regime, cleaned using 2.25% NaOCl+17% EDTA, group 2 and group 6: CP activated by PDT, Group 3 and Group 7 using ECL, Group 4 and Group 8: KTP laser. Following disinfection samples in groups 1, 2, 3, and 4 were obturated using Sankin apatite root canal sealer (SARS). Specimens in groups 5, 6, 7, and 8 were obturated using Pro-root endo sealer (PRES). Sectioning of samples was performed. The sectioned specimens were subjected to a compressive loading exerted via a steel plunger moving at a 0.5mm/min crosshead speed till debonding. Means and standard deviations (SD) of EBS of BC sealer with the root canal dentin were assessed using one-way analysis of variance (ANOVA) and Post Hoc Tukey multiple comparison tests. The level of significance was measured at p=0.05. The coronal section of samples in group 5 (Na-E-PRES) exhibited the highest bond value. The lowest PBS was displayed by the middle third of specimens in group 6. Group 1 (Na-E-SARS), group 3 (Na-E-ECL-SARS), group 5 (Na-E-PRES), and group 7 (Na-E-ECL-PRES) exhibited comparable bond values (p>0.05). Whereas, group 2 (Na-E-PDT-SARS) and group 4 (Na-E-PTPL-SARS), group 6 (Na-E-PDT-PRES), and group 8 (Na-E-PTPL-PRES) were found to be comparable (p>0.05). Adjunctive use of Er, Cr: YSGG laser displayed better extrusion bond strength with BC root canal sealer compared to CP activated by PDT and KTP laser. Bond strength is influenced by the type of irrigation.

Efficacy of curcumin-mediated photodynamic therapy for root canal therapy procedures: A systematic review.

This systematic review aimed to investigate the effectiveness of CUR-mediated PDT (Curcumin mediated PDT) as an adjunct to conventional chemo-mechanical debridement and/or standard PDT of the RC system with endodontic infections. The focused research question was: "Whether the application Curcumin mediated PDT as an adjunct is more effective than the traditional chemo-mechanical debridement and/or standard PDT of the RC system alone for improving antibacterial and/or mechanical features among subjects undergoing RCT?". An electronic literature search was performed in Scopus, PubMed, and Web of Science. In vitro reports utilizing Curcumin mediated PDT as an adjunct to conventional chemo-mechanical debridement considering permanent dentition assessing the antibacterial and/or mechanical effect were included. Eighteen articles were included in the review, out of which 13 studies assessed the antibacterial activity, while 5 evaluated the mechanical properties. Most of the studies concluded that Curcumin mediated PDT had a significant antibacterial activity than the conventional chemo-mechanical debridement and/or standard PDT. Four of the five studies suggested that Curcumin mediated PDT had no impact on the push-out bond strength of root dentin. Furthermore, the significant heterogeneity in the data from the included studies did not permit the author to carry out a meta-analysis. There is potential for application of Curcumin mediated PDT as an adjunct to the conventional chemo-mechanical debridement and/or standard PDT in reducing the bacterial load, however, Curcumin mediated PDT has minimal effect on enhancing the pushout bond strength of fiber posts to radicular dentin. Moreover, clinical studies are required to provide a more conclusive opinion on the efficacy of Curcumin mediated PDT for RCT procedures.

An In-Vitro Analysis of Root Fracture Strength Using Single File Systems.

Aim Root canal obturation during endodontic therapy supports the root canal space and helps the extra tooth structure resist fracture.Some believe endodontic-treated teeth are more likely to break than natural teeth. The most common causes of tooth decay are endodontic treatment's extensive tooth structure loss and coronal and radicular dentin drying. Materials and methods Two hundred removed human permanent mandibular first molars were allowed to be stored in isotonic saline solution for a maximum of 72 hours. The collection, storing, sterilizing, and handling of the samples were done per the Occupational Safety and Health Administration (OSHA) and Centers for Disease Control and Prevention(CDC)guidelines. Out of a total of 200 newly removed mandibular first molars, 120 teethwere finally gathered, sterilized, and kept in 1% thymol in normal saline at 30 degrees Celsius. The access cavity was prepared, and the pulp chamber was cleaned and debridedusing an ultrasonic scaler tip while being irrigated with regular saline. A digital radiograph was taken after a 6# K file was placed to the working length in the mesiobuccal canal.Based on their weights, the samples were dispersed equally across the sixgroups (n=20). They looked inside them to ensure that the root morphology was normal and that the canal was open and free of any abnormalities, damage, or fillings. They looked at the curvature of the mesial root and chose samples with a curvature of 20-35 degrees. The mesial roots were dissected, labeled, and put in a different location. Results Overall, the experimental group'sincidence of buccolingual fractures was 55%, making it the most prevalent fracture type. The mesiodistal type of fracture had a 35% incidence rate, which was the second most prevalent. We found that comminuted and transverse fractures occurred in only 15% and 5% of patients, respectively, of all fractures.Both the test and the control groups had a disproportionately high number of buccolingual fractures. When comparing the root fracture loads of the two experimental groups, there was no significant difference (p>0.05) Conclusion Within this study's restrictions and standardization techniques, it can be concluded that the single file system-prepared roots' resistance to fracture was comparable to that of the control group. It is recommended to conduct additional research on these single file systems using different metrics and to assess them in a clinical setting.

Antimicrobial Activity and Cytotoxicity of Nonsteroidal Anti-Inflammatory Drugs against Endodontic Biofilms.

Persistent infections have become a challenge in dentistry because of growing antibiotic resistance. Nonsteroidal anti-inflammatory drugs (NSAIDs) appear to be a therapeutic alternative to control biofilm infection. The objective of this work is to evaluate the antimicrobial activity and cytotoxicity of sodium diclofenac (DCS), ibuprofen (IBP) and ibuprofen arginine (IBP-arginine) solutions against endodontic polymicrobial biofilms. Sterile radicular dentin blocks of 4 mm × 4 mm × 0.7 mm were used as substrate to grow biofilm. The dentin blocks were submerged into solutions for 5 min. The antimicrobial activity was evaluated by means of the adenosine triphosphate (ATP) assay and confocal laser scanning microscopy (CLSM). Fibroblasts 3T3-L1 (ECACC 86052701) were used to test the cytotoxicity of irrigating solutions. The antibiofilm effects determined by the ATP assay showed that 4% IBP-arginine solution exerted the highest antibiofilm activity, followed by 4% DCS and 4% IBP, with statistical differences among groups (p < 0.001). As for CLSM, 4% DCS and 4% IBP-arginine solutions gave the lowest viable cell percentages, without significant differences between them. Cytotoxicity results at 1/10 dilution were similar for all solutions. At 1/100 dilution, a 4% DCS solution obtained the lowest cell viability for both time periods assayed, 1 h and 24 h. The IBP-arginine group showed the highest cell viability at 24 h. In this preliminary study, in terms of antibiofilm activity and cytotoxicity, a mixed 4% IBP-arginine solution gave the most promising results. NSAID solutions could be recommendable drugs for endodontic disinfection procedures.

Pushout Bond Strength of Root Fillings after Irrigation of Root Canals Utilizing Sodium Hypochlorite, Chlorhexidine, and Homeopathic Mother Tincture (Arnica Montana)

The pushout bond strength of root fillings at radicular dentin was investigated employing NaOCl, CHX, and homoeopathic mother tincture (Arnica montana) as an irrigant. Sixty human permanent single-rooted extracted teeth were decoronated. The root canals were instrumented using Pro taper universal rotary system (Dentsply Tulsa Dental; Tulsa, Oklahoma) and were prepared up to F3 apical size. The roots were then randomly divided into three groups according to irrigation solution (n = 20) according to the final irrigation regimen: Group I: 3 mL 5.25% NaOCl followed by 3 mL Saline (control); Group II: 3 mL Arnica montana (10%, w/v) followed by 3 mL Saline; Group III: 3 mL CHX followed by 3 mL Saline. The canals were dried using paper points. The canals were coated with AH Plus sealer (Dentsply DeTey, Konstaz, Germany) with the aid of a Lentulo spiral (Dentsply DeTey, Konstaz, Germany) and obturated with #F3 gutta-percha. Each root was then horizontally sliced into three slices, labelled coronal, middle, and apical, each measuring 2 mm thick. Furthermore, at a crosshead speed of 2 mm/min, the test was carried out using the universal testing apparatus. The 5.25% NaOCl significantly decreased the bond strength of AH Plus to dentin. Both CHX and Arnica montana were capable of reversing the compromised pushout of AH Plus to NaOCl-treated dentin. After using NaOCl as an irrigant, the danger of diminished binding capacity of AH Plus to root canal walls arises. Final irrigation with Arnica montana and CHX reduces this risk.

Effect of Final Irrigation Protocols on Radicular Dentin Permeability and Push-Out bond Strength in Endodontically Treated Teeth

This study aimed to analyze the effect of several final irrigation protocols on tubular permeability and push-out bond strength of an epoxy resin-based cement to radicular dentin. The crowns of 110 human teeth were removed to standardize at 17mm in length. The root canals were instrumented and irrigated with 5ml of 5% NaOCl and 17% EDTA. The samples were then randomly divided into 5 groups (n=22) according to the final irrigation protocol. Subsequently, the samples were subdivided into two different groups to assess dentin permeability or the evaluation of the push-out bond strength. In Group 1, all samples displayed the formation of brown-like precipitates. Group 2 and Group 5 demonstrated cleaner dentin walls. The push-out bond test after 72 hours denoted a significant difference when comparing Group 1 vs Group 2 and Group 1 vs Group 3. After thermocycling, a significant difference is observed between Group 1 and all remaining groups. The irrigation protocol using distilled water prior to irrigation with chlorhexidine significantly reduces the formation of intracanal precipitates by avoiding interactions between irrigants. The push-out bond strength of epoxy resin-based cement is directly influenced by the precipitates formed in the root canal after irrigation and the time of thermocycling.

Effect of resveratrol pretreatment of radicular dentin on immediate and delayed pushout bond strength of fiber post luted using self-adhesive resin cement: An in vitro study.

To assess and compare the effect of resveratrol pretreatment of root dentin on immediate and delayed pushout bond strength (POBS) of fiber postluted using self-adhesive resin cement. Postspace was prepared in endodontically treated roots and randomly divided into two groups based on pretreatment rinse before fiber postcementation: Group 1: Distilled water Group 2: 1 mL of 10 mg/mL resveratrol solution for 1 min. Fiber posts were luted using self-adhesive resin cement. Among, two 1 mm slices of the coronal and middle third of root, one slice was subjected to immediate POBS using the universal testing machine and the other slice was subjected to POBS after storing in distilled water for 12 months. Resveratrol pretreatment showed significantly higher immediate POBS with more mixed failure than distilled water group. There was no significant difference in delayed POBS between the distilled water (mean: 7.07 MPa) and resveratrol pretreated group (mean: 8.20 MPa) with predominantly adhesive failure among them. There was significant difference between immediate and delayed POBS value of both the groups. Resveratrol pretreatment can be considered to improve bond strength stability of radicular dentin to self-adhesive resin cement.