Quantitative Changes Research Articles

Changes in retinal vascular density measured by optical coherence tomography angiography in patients with carotid artery stenosis after carotid artery stenting and angioplasty.

The aim of this study was to compare the effect of carotid artery stenting and angioplasty (CASA) on retinal vascular density (VD) in patients with severe carotid stenosis with a healthy control group and to evaluate using optical coherence tomography angiography (OCTA). For this prospective study, eyes on the operated side constituted the ipsilateral eye group, and the other eye constituted the contralateral eye group. 40 eyes of 40 patients with ipsilateral eye of carotisid artery stenosis (CAS), 34 eyes on contralateral side, and 30 healthy eyes (control group) were included in this study. We performed quantitative OCTA analyses of retinal VD changes, before and after CASA. The main outcome measures were the quantitative changes of VD of superficial capillary plexus (SCP) and deep capillary plexus (DCP). We evaluated the VD of ipsilateral eyes and contralateral eyes separately before and after the procedure. All patients did not have visual symptoms. There was no significant difference in the VD of SCP in all groups before the procedure. No significant change was observed in all groups when the VD of the SCP was compared before and after the procedure. The VD of the DCP in the ipsilateral and contralateral group improved significantly after CASA. OCTA could noninvasively detect retinal VD improvements after CASA in CAS patients. Quantitative changes in VD evaluated using OCTA are thought to be early indicators in the diagnosis of CAS and in the follow-up of treatment success.

Temporal and spatial variations of groundwater quantity and quality for drinking and irrigation purposes in the arid and hot weather of Southern Iran

Groundwater provides a reliable water supply of fresh water worldwide for drinking, agricultural, and industrial purposes, especially in areas where surface water is scarce or unreliable, making it an essential resource for human and economic development. Proper management and protection of groundwater resources are crucial to ensure their sustainability and continued contribution to fulfilling the growing water demand worldwide. This study focused on the quantitative and qualitative changes in groundwater in the Kahoorestan Plain, located northwest of the Persian Gulf in southern Iran. The zoning maps produced by Arc GIS revealed a declining trend in the aquifer level and a drop in the groundwater level of 5.24 m over 20 years. The maps of the flow directions also indicated a general trend from the western highlands to the south and southeast regions of the plain. The Piper diagram showed that the predominant hydrochemical facies of the plain area is NaCl. Temporal changes analysis by the Mann-Kendall test revealed that EC, TDS, TH, Na, Mg, Ca, SO4, and Cl parameters have a rising trend in the majority of wells, which attributes to groundwater over-withdrawal, drought, excessive well drilling, and insufficient aquifer recharge. The study’s analysis of the temporal and spatial changes of the water quality index, Schoeller diagram, and Wilcox diagram revealed that the aquifer’s water quality is unsuitable for drinking and irrigation use due to its high salinity and sodium content. The findings underscore the urgency of adopting effective management strategies to ensure the sustainable management of groundwater resources in the region.

State primary special standard of acceleration unit in the field of gravimetry GET 190-2023: reproduction and transmission of the unit under the influence of geophysical factors

The relevance of research on the reproduction and transmission of the acceleration unit in gravimetry is determined by the development of measuring instruments for the absolute value of the acceleration of free fall and its changes. Qualitative and quantitative changes in the instrument base are due to the requirements of applied tasks solved using gravimetric data in such fields as geodesy, navigation, geodynamics, as well as the expansion of the field of practical application of absolute gravimeters. At the same time, in order to solve applied problems, along with accuracy requirements at a level close to the maximum achievable at the current level of technology development, maximum territorial coverage of measurement sites within the entire territory of the Russian Federation is often necessary. The accuracy of the results obtained with the help of measuring instruments is determined by the level of their metrological support, the main stages of which are the reproduction of the corresponding unit by the standard and its transfer to the measuring instrument. An analysis of possible sources of errors in gravimetric equipment has shown that when reproducing and transmitting the acceleration unit in gravimetry, it is necessary to take into account the influence of geophysical factors that manifest themselves as additional accelerations of a gravitational or inertial nature. The distribution of the gravitational field within a gravimetric point can manifest itself as an additional constant acceleration. Seismic processes and lunar and solar tides manifest themselves as variable accelerations. For various stages of metrological support of gravimetric devices, the mechanisms of the effects of such accelerations have been studied, as well as methods for accounting and reducing their influence using additional equipment have been developed. An additional gravimetric point with a cryogenic relative gravimeter and a broadband seismometer, as well as transported absolute ballistic and relative quartz gravimeters, were introduced into the State primary special standard of acceleration units in the field of gravimetry GET 190-2023.

Imaging quantitative changes in blood-brain barrier permeability using [18F]2-fluoro-2-deoxy-sorbitol ([18F]FDS) PET in relation to glial cell recruitment in a mouse model of endotoxemia.

The quantitative relationship between the disruption of the blood-brain barrier (BBB) and the recruitment of glial cells was explored in a mouse model of endotoxemia. [18F]2-Fluoro-2-deoxy-sorbitol ([18F]FDS) PET imaging was used as a paracellular marker for quantitative monitoring of BBB permeability after i.v injection of increasing doses of lipopolysaccharide (LPS) or vehicle (saline, n = 5). The brain distribution of [18F]FDS (VT, mL.cm-3) was estimated using kinetic modeling. LPS dose-dependently increased the brain VT of [18F]FDS after injection of LPS 4 mg/kg (5.2 ± 2.4-fold, n = 4, p < 0.01) or 5 mg/kg (9.0 ± 9.1-fold, n = 4, p < 0.01) but not 3 mg/kg (p > 0.05, n = 7). In 12 individuals belonging to the different groups, changes in BBB permeability were compared with expression of markers of astrocyte (GFAP) and microglial cell (CD11b) using ex vivo immunohistochemistry. Increased expression of CD11b and GFAP expression was observed in mice injected with 3 mg/kg of LPS, which did not increase with higher LPS doses. Quantitative [18F]FDS PET imaging can capture different levels of BBB permeability in vivo. A biphasic effect was observed with the lowest dose of LPS that triggered neuroinflammation without disruptive changes in BBB permeability, and higher LPS doses that increased BBB permeability without additional recruitment of glial cells.

A comparison among oscillometric waveforms in healthy nonpregnant women, pregnancy and hypertensive disorders of pregnancy.

Understanding of how oscillometric waveforms (OMW) vary between pregnant and nonpregnant individuals remains low. An exploratory analysis was completed to assess for quantitative and qualitative changes in OMW and oscillometric envelope features in pregnancy. Eighteen pregnant individuals (over 20 weeks gestational age) and healthy, nonpregnant (HNP) women were recruited. Six HNP were matched to six healthy pregnant (HP) women, and six pregnant women with a hypertensive disorder of pregnancy (HDP) by age, arm circumference, and cuff size. Blood pressure measurements were completed per the International Organization for Standardization (ISO) protocol using a custom-built oscillometric device as the test device and two-observer mercury auscultation as the reference measurement. Auscultatory blood pressure and blood pressure derived from slope-based and fixed ratio algorithms were determined. OMW and envelope features were compared among groups. In HNP, HP, and HDP groups respectively: mean auscultatory blood pressure (systolic mean ± SD/diastolic mean ± SD) was 103.4 ± 12.2/67.1 ± 7.9; 109.5 ± 3.1/58.1 ± 6.4; 135.6 ± 18.9/85.1 ± 14.2 mmHg. HDP had significantly higher auscultatory systolic and diastolic blood pressure than the HP group (P = 0.001). The pregnant groups had a lower average pulse width (mean ± SD: HNP = 0.8 ± 0 s, HP = 0.6 ± 0.1 s, HDP = 0.6 ± 0.1 s; HP vs. HNP mean difference [adjusted P value]: 0.2 [P = 0.004], HDP vs. HNP 0.1 [P = 0.018]) compared with the HNP group. The HDP group had a larger area under the OMW envelope than the HNP group (mean ± SD: HNP = 22.6 ± 3.4; HDP = 28.5 ± 4.2; HDP vs. HNP mean difference [adjusted P value]: 5.9 P = 0.05). In this exploratory work, differences in the OMW morphology and parameters were found in pregnancy and in hypertensive disorders of pregnancy compared with healthy controls. Even small differences may have important implications in algorithm development; further work comparing OMW envelopes in pregnancy is needed to optimize the algorithms used to determine blood pressure in pregnancy.

Poincaré analysis detects pathological limb loading rate variability in post-anterior cruciate ligament reconstruction individuals

BackgroundPost-ACLR individuals can experience repeated exposure to variable limb loading, which contributes to development of knee osteoarthritis. Variable limb loading can present as loading rate variability (LRV) and is magnified during tasks like fast walking when the system is stressed. Nonlinear measures that evaluate temporal variability have successfully detected changes in gait variability associated with altered motor control, however, appropriately describing and uncovering the nature of gait variability has been challenging. Here, Poincaré analysis, a nonlinear method unique in its ability to capture different aspects of variability, served to uncover and quantify changes in limb LRV. It was hypothesized that post-ACLR individuals’ overloaded limbs would quantitatively and graphically demonstrate greater short-term stride-to-stride and long-term limb LRV during fast walking compared to the underloaded and healthy control limbs. MethodsFourteen post-ACLR individuals and fourteen healthy controls completed a walking protocol on an instrumented treadmill where they walked at 1.0 m/s and 1.5 m/s for 5-minutes each. A Welch’s test was performed to compare differences in short-term and long-term LRV metrics for the post-ACLR individuals’ overloaded and underloaded limbs and the healthy controls’ right limbs. ResultsAnalyses revealed that the post-ACLR individuals’ overloaded limb exhibited significantly greater short-term and long-term values compared to the underloaded and healthy control limbs at 1.5 m/s (p<0.05). Additionally, the loading rate data was widely scattered across the plots for the overloaded limb, indicating greater LRV. SignificancePoincaré analysis successfully identified that post-ACLR overloaded limbs exhibited impaired motor control during fast walking based on quantitative and graphical changes in variability. This highlights the clinical applications of Poincaré analysis, with the plots potentially serving as an easy-to-interpret diagnostic tool for pathological limb LRV.

Imaging Transcriptomics of the Brain for Schizophrenia.

Schizophrenia is a severe mental disorder with a neurodevelopmental origin. Although schizophrenia results from changes in the brain, the underlying biological mechanisms are unknown. Transcriptomics studies quantitative expression changes or qualitative changes of all genes and isoforms, providing a more meaningful biological insight. Magnetic resonance imaging (MRI) techniques play roles in revealing brain structure and function. We give a narrative focused review on the current transcriptome combined with MRI studies related to schizophrenia and summarize the research methodology and content of these studies to identify the research commonalities as well as the implications for future research, in an attempt to provide new insights into the mechanism, clinical diagnosis, and treatments of schizophrenia.

Quantitative indicators of the training of cadets of the Irkutsk Fire Technical School in 1968-1993.

The subject of the research of the scientific article is the peculiarities of the process of training firefighters on the basis of the Irkutsk Fire Technical School of the Ministry of Internal Affairs of the USSR during the period of activity of this educational institution from 1968 to 1993 on the territory of the Irkutsk region. In this publication, on the basis of previously unpublished and not introduced into scientific circulation archival materials, historical data on quantitative changes in the recruitment and training of cadets of the Irkutsk Fire Technical School during the period of their educational activities are investigated. The author considers the issues of organizing entry examinations for incoming applicants, notes significant events in the educational activities of cadets, and summarizes data on the number of specialists who have completed training. The article identifies the features of the educational process on the basis of the school, which allowed the formation of a professional staff of firefighters in the regions of Siberia and the Far East. The research methodology includes a concrete historical approach (M.V. Astakhov, I.D. Kovalchenko, V.F. Kolomiytsev, A.P. Pronstein, etc.), as well as generalization, comparison, synthesis, classification, concretization; analysis of archival documents, legislation and scientific literature; retrospective analysis; method of historical analogies. The novelty of this scientific article lies in the fact that during the scientific research, historical and pedagogical sources, archival data describing in detail the history of the formation and development of the cadet recruitment structure of the school, as well as a detailed report on the quantitative and qualitative indicators of graduates of the fire school by year of graduation were identified and summarized. The conducted research is of interest to a wide range of readers, since the study of the educational activities of the school, structural and organizational measures for the recruitment and graduation of firefighters is a very important component in the study of the formation and development of departmental education of the Ministry of Internal Affairs of the USSR in the territory of the Baikal region in the XX century. The list of heads of these structural divisions at the stage of formation and development of the Irkutsk Fire Technical School of the Ministry of Internal Affairs of the USSR is considered.

Whole-lesion assessment of volume and signal changes after sclerotherapy of extremity venous malformations

PurposeTo investigate quantitative changes in MRI signal intensity (SI) and lesion volume that indicate treatment response and correlate these changes with clinical outcomes after percutaneous sclerotherapy (PS) of extremity venous malformations (VMs). MethodsVMs were segmented manually on pre- and post-treatment T2-weighted MRI using 3D Slicer to assess changes in lesion volume and SI. Clinical outcomes were scored on a 7-point Likert scale according to patient perception of symptom improvement; treatment response (success or failure) was determined accordingly. ResultsEighty-one patients with VMs underwent 125 PS sessions. Treatment success occurred in 77 patients (95 %). Mean (±SD) changes were –7.9 ± 24 cm3 in lesion volume and –123 ± 162 in SI (both, P <.001). Mean reduction in lesion volume was greater in the success group (–9.4 ± 24 cm3) than in the failure group (21 ± 20 cm3) (P =.006). Overall, lesion volume correlated with treatment response (ρ = –0.3, P =.004). On subgroup analysis, volume change correlated with clinical outcomes in children (ρ = –0.3, P =.03), in sodium tetradecyl sulfate–treated lesions (ρ = –0.5, P =.02), and in foot lesions (ρ = –0.6, P =.04). SI change correlated with clinical outcomes in VMs treated in 1 PS session (ρ = –0.3, P =.01) and in bleomycin-treated lesions (ρ = –0.4, P =.04). ConclusionsChange in lesion volume is a reliable indicator of treatment response. Lesion volume and SI correlate with clinical outcomes in specific subgroups.

IL-4-induced decrease in both the number and CTLA-4 expression of Treg impairs suppression of Th2 type inflammation in severe atopic dermatitis

BackgroundTreg plays a pivotal role in the suppression of Th2 cell and the maintenance of immune homeostasis. The precise molecular mechanism underlying the disruption of Treg suppression of Th2 cell and the promotion of Th2 type inflammation in allergic diseases remains elusive. ObjectiveThis study aims to investigate the molecular mechanism underlying quantitative and functional changes of Treg in AD. MethodsThe molecular mechanism was investigated using flow cytometry, mRNA sequencing, co-culture experiments, co-immunoprecipitation, chromatin immunoprecipitation, and bisulfite sequencing in vitro or in AD mice model and patients with AD. ResultsIncreased proportion of Treg was detected in mild and moderate AD. Conversely, characteristic decrease in both the number and CTLA-4 expression of Treg was relevant to serum IL-4 level in severe AD patients, which was verified under a high concentration of IL-4 treatment in vitro. The underlying mechanism is that IL-4/pSTAT6 pathway recruits DNMT1 and HDAC2 to inhibit transcriptional regulation of Foxp3 and CTLA-4 loci. High level of IL-4 impaired the suppression of Treg against Th2 cell differentiation mediated by CTLA-4, and blockade of IL-4Rα signaling in Treg restored Treg number and suppression of Th2 cell in AD model mice and patients with AD. ConclusionThe number of Treg is relevant to stratification of severity and serum IL-4 level in patients with AD. Abnormal high level of IL-4 epigenetically triggers a decrease in both the number and CTLA-4 expression of Treg. The reduced expression of CTLA-4 on Treg induced by IL-4 impairs suppression of Th2 cell differentiation.

Monitoring of key Camellia Oleifera phenology features using field cameras and deep learning

A rapid and accurate yield estimation is of great significance to the management and sustainable development of Camellia Oleifera forests. Consequently, the simultaneous and accurate detection of key phenology features of Camellia Oleifera (buds, flowers, fruits) is crucial for precise yield estimation. This not only enables robotic harvesting but also allows for the prediction of peak flowering and fruit ripening periods to determine the optimal harvesting time. However, in recent studies, only Camellia Oleifera fruits have been marked and detected. Therefore, to enable rapid yield estimation, it is necessary to simultaneously detect the key phenology stages (buds, flowers, fruits) of Camellia Oleifera. In this study, we annotated, trained, and predicted Camellia Oleifera buds, flowers, and fruits collected via field cameras to observe their daily quantitative changes. Quantity change curves were generated to estimate crucial phenology stages. Phenology feature detection and transfer learning were performed using the YOLO v5 model, widely used YOLO v3 model, and CenterNet model with center point prediction, all trained on the same dataset. The best model for phenology feature detection was selected based on a comparison of average precision, with the YOLO v5 model achieving a higher mean Average Precision (mAP) value of 91.31 % compared to the CenterNet (85.43 %) and YOLO v3 (81.00 %) models. In YOLO v5, the AP values for bud, flower, and fruit detection were 82.80 %, 98.13 %, and 92.99 %, respectively, surpassing the CenterNet model by 3.97 %, 2.44 %, and 11.23 %, and the YOLO v3 model by 6.39 %, 17.13 %, and 11.67 %. The image size was adapted from 4000 × 3000 pixels to 512 × 512 pixels for model optimization. Additionally, data from the Seedling Center of Liuyang City collected at different years and times were utilized to showcase the generalizability and scalability of the selected models, resulting in mAP values of 86.14 %, 80.17 %, and 69.20 % for the three above-mentioned models respectively. The plotted phenology change curves unveiled that Camellia Oleifera undergoes four stages: fruit enlargement period, bud enlargement period, flowering period, and flower wilting period. The conclusion can be drawn that using field cameras and YOLOv5 can simultaneously achieve real-time detection of key phenology features (buds, flowers, and fruits) of Camellia Oleifera, in order to further record crucial phenology patterns (such as flowering peaks and fruit ripening periods). This study offers theoretical references and scientific evidence for monitoring changes in key phenology features of Camellia Oleifera.

THE STUDY OF THE GENESIS OF STATE MECHANISMS TO STIMULATE THE DEVELOPMENT OF THE REGIONAL SECTOR OF THE ECONOMY OF SMALL AND MEDIUM-SIZED BUSINESSES

Purpose of the study. The article examines the genesis and current state of civil law mechanisms aimed at stimulating qualitative and quantitative changes in the structure of regional small businesses participating in the innovative development of the Russian economy. From a critical point of view, the evolution of domestic legislation of the business entities under consideration is analyzed, scientific and practical qualifications of the key stages of transformation of legislative initiatives in the area under study are reasonably proposed, the most significant risks characterizing external threats to the necessary dynamics of small business development are objectively studied. Conclusions. As a result of the research, the author comes to the conclusion that since a distinctive feature of the national economy is its pronounced regional character, the development of the macroeconomics of Russia is possible only if, on the one hand, the balanced and interconnected progression of its regions, on the other, the improvement of federal management processes with the aim of improving the quality and validity of the regulatory environment, capable of taking into account the specifics of both territorial and macroeconomic development of the state. This creates a certain level of requirements for the quality of government mechanisms for federal investment in regional SMB.

High-density lipoprotein in diabetes: Structural and functional relevance.

Low levels of high-density lipoprotein-cholesterol (HDL-C) is considered a major cardiovascular risk factor. However, recent studies have suggested a more U-shaped association between HDL-C and cardiovascular disease. It has been shown that the cardioprotective effect of HDL is related to the functions of HDL particles rather than their cholesterol content. HDL particles are highly heterogeneous and have multiple functions relevant to cardiometabolic conditions including cholesterol efflux capacity, anti-oxidative, anti-inflammatory, and vasoactive properties. There are quantitative and qualitative changes in HDL as well as functional abnormalities in both type 1 and type 2 diabetes. Non-enzymatic glycation, carbamylation, oxidative stress, and systemic inflammation can modify the HDL composition and therefore the functions, especially in situations of poor glycemic control. Studies of HDL proteomics and lipidomics have provided further insights into the structure-function relationship of HDL in diabetes. Interestingly, HDL also has a pleiotropic anti-diabetic effect, improving glycemic control through improvement in insulin sensitivity and β-cell function. Given the important role of HDL in cardiometabolic health, HDL-based therapeutics are being developed to enhance HDL functions rather than to increase HDL-C levels. Among these, recombinant HDL and small synthetic apolipoprotein A-I mimetic peptides may hold promise for preventing and treating diabetes and cardiovascular disease.

Skeletal manifestations of anemia in the sternum in a modern clinical sample: An initial investigation

Anemia is a globally significant condition, both today and throughout history. Studying how it affected past communities contributes to understanding its impact on vulnerable groups. However, anemia diagnosis in skeletal remains is challenging, and improving methods for diagnosis is necessary for moving forward. In current clinical practice, sternal bone marrow undergoes anemia-related changes, which could affect underlying bone structure, but the sternum has never been investigated as a skeletal marker of anemia. We used a cohort of individuals with known hematological data (N = 23) to investigate whether there are quantitative bone changes in the sternum of individuals clinically diagnosed with anemia. Sternal bone features were measured using CT imaging to calculate a ratio of the relative cortical bone to trabecular space sample. The sample was separated into non-anemic and anemic cohorts based on World Health Organization diagnostic parameters. We found significant differences in ratios between those with and without anemia, suggesting that sternal bone ratio measurements could be used as a diagnostic parameter. In individuals without clinically-diagnosed anemia, ratios never exceeded 3.6 in the manubrium and 2.3 in the sternal body. We repeated these ratio measurements using micro-CT analysis on a sample of archaeological sternums and found that the ratio diagnostic parameter could also be used in this sample to identify those who could have experienced anemia. These results demonstrate the utility of quantitative methods for diagnosing anemia in skeletal remains and suggest that sternal ratio measurements can be used as part of anemia diagnosis in past contexts.

Состав общественно-политической лексики ингушского языка

The aim of the research is to group the socio-political vocabulary of the Ingush language according to the spheres of its usage. The article is dedicated to the classification of socio-political vocabulary, identifying thematic groups within it. The scientific novelty of the work lies in the classification of lexemes of the socio-political vocabulary of the Ingush language based on the fields of activity in which this lexical layer is used. Such systematization will allow tracking quantitative and qualitative changes in the composition of the socio-political vocabulary of the Ingush language. The research results have shown that lexemes in the socio-political sphere of the Ingush language can be categorized into four groups: administrative-political, socio-economic, military-legal, and religious. It has been established that the socio-political vocabulary of the Ingush language is updated along with changes in the social, political, economic, military, and religious life of society, leading to an expansion of the lexical composition in all the aforementioned spheres. The study revealed that adoption is the primary way for the emergence of new words. Religious lexemes are predominantly borrowed from the Arabic language. Additionally, new words that have appeared in the language in the last decade are borrowed from the Russian language.

Interferon-gamma release assay conversion after Mycobacterium tuberculosis exposure specifically associates with greater risk of progression to tuberculosis: A prospective cohort study in Leicester, UK

ObjectivesWe investigated whether quantifying the serial QuantiFERON-TB Gold (QFT) response improves tuberculosis (TB) risk stratification in pulmonary TB (PTB) contacts. MethodsA total of 297 untreated adult household PTB contacts, QFT tested at baseline and 3 months after index notification, were prospectively observed (median 1460 days). Normal variance of serial QFT responses was established in 46 extrapulmonary TB contacts. This informed categorisation of the response in QFT-positive PTB contacts as converters, persistently QFT-positive with significant increase (PPincrease), and without significant increase (PPno-increase). ResultsIn total, eight co-prevalent TB (disease ≤3 months after index notification) and 12 incident TB (>3 months after index notification) cases were diagnosed. Genetic linkage to the index strain was confirmed in all culture-positive progressors. The cumulative 2-year incident TB risk in QFT-positive contacts was 8.4% (95% confidence interval, 3.0-13.6%); stratifying by serial QFT response, significantly higher risk was observed in QFT converters (28%), compared with PPno-increase (4.8%) and PPincrease (3.7%). Converters were characterised by exposure to index cases with a shorter interval from symptom onset to diagnosis (median reduction 50.0 days, P = 0.013). ConclusionsQFT conversion, rather than quantitative changes of a persistently positive serial QFT response, is associated with greater TB risk and exposure to rapidly progressive TB.

Quantitative and Qualitative Radiological Assessment of Sarcopenia and Cachexia in Cancer Patients: A Systematic Review.

Sarcopenia, an extremely common condition in cancer patients, is described as a progressive and generalized musculoskeletal disorder that is associated with an increased likelihood of adverse outcomes, including falls, fractures, physical disability, and mortality. By contrast, cachexia is defined as a syndrome characterized by weight loss with the concomitant loss of muscle and/or fat mass. Cancer cachexia leads to functional impairment, reduced physical performance, and decreased survival, and is often accompanied by cancer progression and reduced response to therapy. The literature states that cancer patients with cachexia or sarcopenia have many more complications than patients without these conditions. The interplay between physiologic sarcopenia and cancer cachexia is, in part, responsible for the complexity of studying wasting disorders in the cancer population, particularly in the geriatric population. For these reasons, a comprehensive assessment of the body composition and physical function of these patients is necessary. There are several modalities adapted to measure skeletal muscle mass, such as dual-energy X-ray absorptiometry (DEXA), bioelectrical impedance analysis (BIA), computed tomography (CT), magnetic resonance imaging (MRI), and ultrasound (US). The gold standard for the measurement of quantitative and qualitative changes in body composition in patients with cancer is the analysis of tissue density using a CT scan. However, this technique remains poorly implemented in clinical practice because of the use of ionizing radiation. Similarly, DEXA, MRI, and US have been proposed, but their use is limited. In this review, we present and compare the imaging techniques that have been developed so far for the nutritional assessment of cancer patients.

Dynamic root microbiome sustains soybean productivity under unbalanced fertilization

Root-associated microbiomes contribute to plant growth and health, and are dynamically affected by plant development and changes in the soil environment. However, how different fertilizer regimes affect quantitative changes in microbial assembly to effect plant growth remains obscure. Here, we explore the temporal dynamics of the root-associated bacteria of soybean using quantitative microbiome profiling (QMP) to examine its response to unbalanced fertilizer treatments (i.e., lacking either N, P or K) and its role in sustaining plant growth after four decades of unbalanced fertilization. We show that the root-associated bacteria exhibit strong succession during plant development, and bacterial loads largely increase at later stages, particularly for Bacteroidetes. Unbalanced fertilization has a significant effect on the assembly of the soybean rhizosphere bacteria, and in the absence of N fertilizer the bacterial community diverges from that of fertilized plants, while lacking P fertilizer impedes the total load and turnover of rhizosphere bacteria. Importantly, a SynCom derived from the low-nitrogen-enriched cluster is capable of stimulating plant growth, corresponding with the stabilized soybean productivity in the absence of N fertilizer. These findings provide new insights in the quantitative dynamics of the root-associated microbiome and highlight a key ecological cluster with prospects for sustainable agricultural management.

Evaluation of Enamel Volume Loss after Exposure to Energy Drinks

Objectives: This study was conducted to determine the erosive potential of various commercial energy drinks (EDs), sports drinks (SDs), and sugar sweetened beverages (SSBs) and to correlate quantitative changes in tooth enamel volume loss based on the pH and titratable acidity of the drinks. Methods: A flat plane on the facial surface of 36 human incisor teeth was created and embedded in sample holders using resin. After pre-scanning with a profilometer (Proscan 2000, Scantron, Ind Products Ltd., Taunton, UK), the six samples per group were immersed for 4 h into either Monster Energy™ (ED), Rockstar™ (ED), Red Bull™ (ED), or 5-h Energy™ (ED) and, for comparison with a sports drink, Gatorade™ (SD) and a sugar sweetened beverage, Coca-Cola® (SSB). After immersion and post-scanning, the quantitative volume loss of the tooth enamel of the 36 samples was calculated (Proscan 3D software V2.1.1.15B), and the pH and titratable acidity (TA) of each drink was determined. Results: All drinks tested caused enamel volume loss. The actual amount varied among the different drinks, from 0.39 mm3 for Red Bull™, up to 1.01 mm3 for Gatorade™. The pH measurements differed for each drink, ranging from 2.6 to 3.7. There was a small reverse correlation of 0.326 between the pH of all drinks and volume loss. Among the energy drinks, titratable acidity was similar and there was only a weak correlation between TA and volume loss (0.319 at p = 0.53). Conclusions: Energy drinks, sugar sweetened beverages, and sport drinks all have the potential to cause enamel tooth surface loss resulting in demineralization. Therefore, the pH of a drink cannot be the sole determinant for choosing a less harmful commercial beverage.

Personal reformulation during the clinical associate psychologist apprenticeship: Exploratory mixed methods evaluation.

Clinical associate psychologists (CAPs) train under the auspices of the apprenticeship programme and are a new addition to the psychological workforce. This project sought to evaluate whether a "personal reformulation" (PR) was helpful in terms of personal and professional development during the apprenticeship. A mixed methods evaluation containing a longitudinal quantitative element and a "Big Q" qualitative element with a single cohort of N = 18 CAPs. A PR consists of a 2-hr one-to-one session and a follow-up session with a cognitive analytic psychotherapist. During a PR, a sequential diagrammatic reformulation is produced to aid recognition and revision of potentially problematic relationship patterns at work. Two outcome measures concerning reflective capacity and professional quality of life were completed at the start of the apprenticeship, pre-PR and at 3-month PR follow-up. The semi-structured interviews (n = 11) conducted at the follow-up were analysed using reflexive thematic analysis. Quantitative changes were limited to significant increases to general confidence and 8/12 apprentices had a reliable increase in confidence in communication. Qualitatively, five overarching themes were found: (1) gaining insight, (2) wellbeing, (3) nature of the space, (4) being an apprentice, and (5) moving forward. PRs were generally found to be emotionally challenging, but relatively large amounts of insight are possible from a very brief intervention that can contribute to personal and professional development during clinical training. More controlled research needs to be conducted and wider applications and evaluations of PRs in different professions would be welcome.