Qualitative Composition Research Articles

Analysis of bioactive compounds in Cordyceps militaris fruiting bodies and dietary supplements: in vitro bioaccessibility determination in artificial digestive juices

SummaryIn recent years, Cordyceps militaris has garnered increased attention in both scientific research and popular culture, exemplified by references in the entertainment industry, such as ‘The Last Of Us’. This study aims to investigate the qualitative and quantitative composition of bioactive compounds (such as cordycepin, ergothioneine, L‐phenylalanine, lovastatin, sterols, indole and phenolic compounds) in various samples. These samples encompass commercially available dietary supplements (in the form of capsules or powder), featuring standardised extracts or non‐standardised C. militaris material. Additionally, fruiting bodies cultivated in Poland and China were examined. The bioactive compounds were subsequently analysed after extraction into artificial digestive juices. Each sample underwent extraction, evaporation and dissolution in methanol, followed by qualitative and quantitative analyses using RP‐HPLC (with UV and DAD detectors) for the determination of bioactive compounds. The results of this analysis revealed that extracts with cordycepin contents of 7% and 1% exhibited the highest cordycepin concentrations, as well as significant ergothioneine levels (4405 and 928 mg/100 g d.w. and 234 and 77.6 mg/100 g d.w. respectively). However, on analysis of simulated digestive juices, it became evident that the fruiting bodies of C. militaris are superior sources of bioactive compounds. Consequently, this study underscores the efficacy of unprocessed material, such as fruiting bodies, as the preferred form of supplementation for potential consumers of C. militaris.

Biochemical analysis of Hyalomma dromedarii salivary glands and gut tissues using SR-FTIR micro-spectroscopy

Ticks are obligatory voracious blood feeders infesting diverse vertebrate hosts, that have a crucial role in the transmission of diverse pathogens that threaten human and animal health. The continuous emergence of tick-borne diseases due to combined worldwide climatic changes, human activities, and acaricide-resistant tick strains, necessitates the development of novel ameliorative tick control strategies such as vaccines. The synchrotron-based Fourier transform infrared micro-spectroscopy (SR-FTIR) is a bioanalytical microprobe capable of exploring the molecular chemistry within microstructures at a cellular or subcellular level and is considered as a nondestructive analytical approach for biological specimens. In this study, SR-FTIR analysis was able to explore a qualitative and semi-quantitative biochemical composition of gut and salivary glands of Hyalomma dromedarii (H. dromedarii) tick detecting differences in the biochemical composition of both tissues. A notable observation regarding Amide I secondary structure protein profile was the higher ratio of aggregated strands in salivary gland and beta turns in gut tissues. Regarding the lipid profile, there was a higher intensity of lipid regions in gut tissue when compared to salivary glands. This detailed information on the biochemical compositions of tick tissues could assist in selecting vaccine and/or control candidates. Altogether, these findings confirmed SR-FTIR spectroscopy as a tool for detecting differences in the biochemical composition of H. dromedarii salivary glands and gut tissues. This approach could potentially be extended to the analysis of other ticks that are vectors of important diseases such as babesiosis and theileriosis.

A Comparison of Methods of Visual Census and Cryptobenthic Fish Collecting, an Integrative Approach to the Qualitative and Quantitative Composition of the Mediterranean Temperate Reef Fish Assemblages

The present research quantitatively compared the fish composition among two methods for non-cryptic benthic fish species and one method for cryptobenthic fish species for the first time for the Mediterranean temperate reef fish assemblage. A visual census of fishes was performed within a cylinder of 4 m radius and within a cylinder of 2 m radius, while the cryptobenthic fishes were collected using a square of 1 m2 with anesthetic. The data and material were collected at fifty sampling points. The visual census methods together recorded 31 species, and the square with anesthetic method recorded 18 species. The quantitative comparison of methods of visual census and cryptobenthic fish collecting showed significantly different species richness, total fish abundance, and fish assemblage structure among methods. The applied methods were highly complementary. The cylinder of 2 m radius is well suited for epibenthic fishes and the cylinder of 4 m radius is reliable for hyperbenthic and benthopelagic fishes. Therefore, each of the methods well covered one of three components of ichthyobenthos (hyperbenthic, epibenthic, and cryptobenthic fishes), and all three methods together provided a far more complete assessment of fish species composition than any individual census method for the Mediterranean littoral benthic fishes.

RESEARCH OF SPAIN SCORZONERA (SCORZONERA HISPANICA L.) FOR THE DEVELOPMENT OF FUNCTIONAL NUTRITIONAL FOODS BASED ON IT

Introduction. The relevance of the study lies in the study of the chemical composition of the food culture of Scorzonera hispanica of the "Lechebnye" variety, identification of the most significant, from the point of view of therapeutic nutrition biologically active substances and prediction of further possible ways of using raw materials of this plant in the development of functional foods. Purpose of the study. To study the qualitative and quantitative composition of substances determining the nutritional value of Scorzonera hispanic variety Lechebnye cultivated in the Krasnodar region, as well as a detailed study of the amino acid composition of the root and herb, and their water-extractable complexes for subsequent use in the development of food products of functional purpose. Material and method. The object of the study was the roots and grass of the Scorzonera hispanica of the "Lechebnye" variety, harvested in 2021–2023 on the territory of the North Caucasus branch of FGBNU VILAR. Dry water-extractable complexes of Scorzonera herb and root were obtained according to a well-known technological scheme, including the stages of solid-phase extraction, concentration of the resulting extract, purification by filtration and drying of the extract. Physico-chemical indicators of the nutritional value of the root and herb of Scorzonera hispanica: qualitative and quantitative composition of nutrients (protein, fiber, fat, calcium and phosphorus), safety indicators (arsenic, cadmium, were determined using generally accepted methods for food raw materials. Capillary electrophoresis was used as an analytical method for studies of the composition and content of amino acids in the studied objects. Results. It was found that the above-ground and underground organs of cultivated food plant Scorzonera hispanica cultivar Lechyorny in the Krasnodar Territory are characterized by a high content of physicochemical indicators of nutrients such as crude protein, fiber, fat. Thus, the mass fraction of crude protein in the root – 11.41%, in the grass – 10.43%, fiber in the root – 6.6% and 30.0% in the grass, crude fat in the root – 3.28%, in the grass –3.39%. The data of studies of amino acid composition of grass, roots of Scorzonera hispanica and their water-extractable complexes show that grass and root contain 12 amino acids, 7 of which are essential (threonine, valine, methionine, isoleucine, leucine, phenylalanine, lysine). The studied complexes revealed the presence of a number of substituted amino acids - tyrosine, cysteine, serine, proline, alanine, glycine and essential amino acids - phenylalanine, threonine, methionine, lysine, and valine, histidine and arginine, histidine and arginine (essential). Conclusions. The research results obtained allow us to conclude that the herb and roots of Scorzonera hispanica of the "Lechebnye" variety are valuable as sources of essential nutrients, such as protein, fiber, fat, calcium, phosphorus and amino acids, which provides the opportunity to consider the creation of functional food products based on these plant materials.

Influence of Technological Factors on the Formation and Transformation of Iron-Containing Phases in the Process of Ferritization of Exhausted Etching Solutions

Every year, metallurgical enterprises generate a massive amount of toxic exhausted high-concentration etching solutions. Application of the ferritization process to recycle exhausted etching solutions can help to prevent environmental pollution. It enables a cost-efficient use of water at an industrial plant and allows the plant to obtain products from toxic industrial waste and utilize it. The aim of the study was to analyze the qualitative and quantitative composition of the formed sediment and its grain size composition. Variable study parameters were the initial pH values of the solutions, the initial concentrations of total iron, and the duration of the aeration process of the reaction mixture. Thermal activation and alternating magnetic fields were used to activate the ferritization. The XRD showed that the formed sediments contained phases of γ-FeOOH, δ-FeOOH, Fe3O4, and γ-Fe2O3. Granulometry analysis showed that these sediments were highly dispersed and heterogeneous. Chemically stable phases of magnetite were obtained in the composition of sediments, with an initial concentration of iron in the reaction mixture of 16.6 g/dm3, a pH of 11.5, and a process duration of 15 min. The study results demonstrated the feasibility of further study and possible use of such sediments with a high magnetite content for the production of materials with ferromagnetic and sorption properties.

Machine Learning-Based Prediction of Elastic Properties Using Reduced Datasets of Accurate Calculations Results

In this paper, the applicability of machine learning for predicting the elastic properties of binary and ternary bcc Ti and Zr disordered alloys with 34 different doping elements is explored. The original dataset contained 3 independent elastic constants, bulk moduli, shear moduli, and Young’s moduli of 1642 compositions calculated using the EMTO-CPA method and PAW-SQS calculation results for 62 compositions. The architecture of the system is made as a pipeline of a pair of predicting blocks. The first one took as the input a set of descriptors of the qualitative and quantitative compositions of alloys and approximated the EMTO-CPA data, and the second one took predictions of the first model and trained on the results of the PAW-SQS calculations. The main idea of such architecture is to achieve prediction accuracy at the PAW-SQS level, while reducing the resource intensity for obtaining the training set by a multiple of the ratio of the training subsets sizes corresponding to the two used calculation methods (EMTO-CPA/PAW-SQS). As a result, model building and testing methods accounting for the lack of accurate training data on the mechanical properties of alloys (PAW-SQS), balanced out by using predictions of inaccurate resource-effective first-principle calculations (EMTO-CPA), are demonstrated.

Legal regulation of the implementation of federal state control (supervision) in the field of treatment of animals in terms of compliance with the requirements for the maintenance and use of animals for cultural and entertainment purposes

The article analyzes the implementation of legislative requirements in the field of treatment of animals for their use in cultural and entertainment events. Data are provided for the Russian Federation on the number of controlled objects in this area. Data on objects controlled by the North-West Interregional Directorate of Rosselkhoznadzor were analyzed. It is determined by what criteria and what categories of risk of harm to legally protected values include objects that are used by controlled persons when keeping and using animals for cultural and entertainment purposes. The procedure for obtaining a license to carry out the type of activity under study is considered. A statistical analysis was carried out on the qualitative and quantitative composition of controlled objects for a number of subjects belonging to the North-West region. An analysis of the powers of the Federal Service to carry out federal state veterinary control (supervision) on compliance or non-compliance by a controlled person with mandatory requirements is carried out. The range of preventive and control measures, their scope and responsibility for violation of the law have been determined.

Microbiological indicators of cottage cheese using different rennet leavens

Rennet cheeses occupy an important place in the diets of the population of Ukraine. The technology of cheese production depends both on the quality of raw materials and on the quality of the enzymes used to curdle milk. Therefore, the study of the effect of rennet enzymes obtained by improved biotechnology on the microbial state of the finished product is of scientific and practical importance. III groups of samples (n = 3) were formed to conduct the experiment. Cow's milk for research was collected from clinically healthy cows during the calving period. In the control group of samples, rennet enzyme of microbial origin was used for curdling milk. In the 1st experimental group of samples, an enzyme preparation was used, extracted from the rennet of dairy calves according to the method of Yu. Ya. Svyridenko In the II experimental group, an enzyme preparation obtained by the method of extraction according to the method of was used. S. V. Merzlova. Laboratory research was carried out in the conditions of the Research Institute of Food Technologies and Technologies of Processing Livestock Products and the Laboratory of Microbiological Research Methods of the Department of Microbiology of the Bilotserk National Agrarian University, which is certified according to (DSTU ISO 10012:2005). To determine the qualitative and quantitative microbial composition, culture was carried out from cheeses obtained with the help of various enzyme preparations. Microbiological research was carried out using the methods defined by DSaNPiN 4.4.5-078-2001 “Microbiological standards and methods of control of food products”. To determine bacterial insemination, two nutrient media such as Lees agar and Streptococcus thermophilus agar were used. The method of serial dilutions was used for seeding the suspension. Sowing was carried out by applying 1.0 cm³ of suspension (dilutions from 1 to 10-5) in melted and cooled agar in Petri dishes, followed by thermostating for 48 hours at 37 °.

Effect of Magnetite Nanoparticles on Human Blood Components.

The qualitative composition and zeta potential of magnetite nanoparticles (size 4.2±1.2 nm) obtained by co-precipitation method were determined by X-ray and diffraction dynamic light scattering. The zeta potential of Fe3O4 particles was -15.1±4.5 mV. The possibility of interaction of magnetite nanoparticles with human blood plasma proteins and hemoglobin as well as with erythrocyte membranes was demonstrated by spectrophotometry, electrophoresis, and fluorescence methods. No changes in the sizes of hemoglobin molecules and plasma proteins after their modification by Fe3O4 particles were detected. The possibility of modifying the structural state of erythrocyte membranes in the presence of magnetite nanoparticles was demonstrated by means of fluorescent probe 1-anilinonaphthalene-8-sulfonate.

BASIC PROVISIONS OF THE METHODOLOGY FOR DETERMINING THE QUANTITATIVE AND QUALITATIVE COMPOSITION OF FIELD REPAIR TEAMS FOR THE RESTORATION OF DAMAGED WEAPONS AND MILITARY EQUIPMENT OF THE ANTI-AIRCRAFT MISSILE TROOPS OF THE AIR FORCES OF THE ARMED FORCES

Analysis of the performance of combat missions by military units and separate units of the Air Force of the Armed Forces of Ukraine in the area of the Joint Forces operation in the east of Ukraine (anti-terrorist operation in the Donetsk and Luhansk regions) and during the full-scale armed aggression by the russian of the russian federation indicates a significant change in the modern forms and methods of use of troops (forces), which leads to the need to improve existing and find new ways of performing logistical support tasks, in particular, the restoration of damaged samples of weapons and military equipment. In the main provisions of the well-known methodology for determining the quantitative and qualitative composition of field repair brigades for the restoration of weapons and military groups of anti-aircraft missile units of the Air Force of the Armed Forces of Ukraine, damaged during the conduct of hostilities, the specifics of combat operations of units of anti-aircraft missile troops in modern armed conflicts and factors that can to influence the organization and implementation of logistical support for the repair and restoration of anti-aircraft missile weapons. A methodology for determining the quantitative and qualitative composition of field repair teams for the restoration of damaged weapons and military equipment of the Air Force of the Armed Forces of Ukraine was developed and proposed. The methodology is aimed at the use of logistical support bodies when conducting operational-tactical and logistical calculations at the stages of early, direct planning and conducting combat operations. The methodology is one of the components in the creation of a methodological apparatus for the functioning of the system of restoring weapons and military equipment, the process of supporting the decision on the logistical support of troops (forces) during the preparation and conduct of hostilities (military operations). This technique can be used as a basis for the development of similar techniques for assessing the possibility of restoring the armament and military equipment of other branches of the Air Force of the Armed Forces of Ukraine.

Studying the acids and amino acid composition of horse chestnut flowers

Introduction. Currently, there is an increasing trend towards assessing the full spectrum of chemical compounds in plant materials. One of the first steps in the formation of secondary metabolites of complex structure, participating in biochemical processes in the plant body, which have their own effect on the human and animal body, are organic acids (OA) and amino acids (AA). However, due attention is not always given to these compounds when studying the component composition of plant raw materials.Aim. The purpose of the work was to study the profile and quantitative assessment of the content of organic acids and amino acids of Aesculus hippocastanum L. flowers.Materials and methods. The object of the study were horse chestnut flowers, collected during flowering in the Voronezh region in 2021 and dried by air-shade method. Quantitative assessment of the amount of TC in terms of malic acid and ascorbic acid was carried out titrimetrically according to the methods presented in the State Pharmacopoeia of the Russian Federation, XIV edition. The composition of the OA and AA profiles of horse chestnut flowers was determined using the capillary electrophoresis method ("Kapel®-105/105M", Group of Companies "Lumex", Russia).Results and discussion. The method of capillary electrophoresis revealed the presence of 12 compounds of the OA group in a total amount of 4.7 % (in terms of absolutely dry raw materials). The rationality of recalculating the content of the sum of OA to the major component – citric acid, which amounted to 1.65 ± 0.04 %, was shown. The content of ascorbic acid was low (0.033 ± 0.007) %. Using the TLC method, the presence of 5 zones of compounds belonging to the AA class was established in the aqueous extract of horse chestnut flowers. The quantitative content of the sum of free AA in terms of glutamine, determined by the spectrophotometric method, was 2.25 ± 0.07 % (n = 5). The presence of 17 AA was determined by capillary electrophoresis, which amounted to 7.13 %. Glutamic acid is present in the greatest quantity in horse chestnut flowers (1.19 %). Essential amino acids amounted to 2.27 %; leucine was present in the predominant amount (0.58 %).Conclusion. A study was carried out of the qualitative composition and quantitative content of organic acids and amino acids of horse chestnut flowers. The composition of the profile and the quantitative content of organic acids and amino acids have been established. It was revealed that citric acid prevails in the total organic acids; glutamic, aspartic acid, arginine and proline are found in greater quantities in the total amino acids.

Сучасний стан висотної мережі України, дослідження технічних характеристик нівелірних знаків

The goal is to determine the current state of points of height networks of I and II classes based on the analysis of technical characteristics of leveling points and regulatory documents with the aim of presenting the scientific and industrial geodetic community with author’s proposals. Method. The following methods of scientific knowledge – analysis, toponymic, etymological, statistical, systematic, historical, monographic, as well as methods – generalization, abstraction, concretization, were used to process the information on the technical characteristics of leveling points, presented on the geoportal of the State Government of Ukraine. The results. The quantitative and qualitative composition of leveling networks of the I and II classes is given, which showed together the technical and technological inconsistency of regulatory documents with the current state of the leveling (altitude) network, which necessitates the use of complex methods for monitoring leveling points on the territory of Ukraine. Scientific novelty. A comprehensive analysis of the information source on the state (technical characteristics) of leveling networks of I and II classes, which is the geoportal of the State Ministry of Ukraine, with the analysis of the provisions of the relevant regulatory documents, is the basis for the development of scientific and technical proposals for the long-term operation of leveling points of the State Ministry of Ukraine and will allow to identify weak aspects of their functioning caused by changes in their surrounding environment. The practical significance of the work shows the inconsistency of the provisions of the instructions and orders during monitoring of the state of leveling points and. accordingly, during the preparation of reporting documentation, which is due to the large number of types of centers, benchmarks of brands in leveling networks of I and II classes, as well as a significant amount of available at all levels, different in terms of physical and historical information about the surrounding environment of leveling points, proposals are proposed to solve the scientifically identified – technical contradictions when using leveling networks of I and II classes. The basis of the scientific proposals is the long-term scientific research conducted by the authors.

Gc-ms analysis of leaf and rhizome essential oil of zingiber purpureum roxb. From bangladesh

Essential oils (EOs) are among the most important plant secondary metabolites. Zingiber purpureum Roxb. is a tropical ginger from Bangladesh, but its chemical constituents of leaves and rhizomes, both EOs, may have pharmacological effects. The aim of this study was carried out to analyze the qualitative composition of both Essential oils (EOs) of both leaf and rhizomes of Z. purpureum. The EOs from the leaves and rhizomes were extracted by hydrodistillation. Chemical constituents of the EOs were separated and identified by gas chromatography/mass spectrometry (GC/MS), and the relative percentage of each constituent was determined by area normalization. In total, 81 compounds were identified from leaf EOs, whereas 44 were from rhizome EOs. The common compounds identified from both rhizome and leaf EOs were triquinacene, 1,4, bis (methoxy), b-phellandrene and b-sesquiphellandrene, respectively. Besides, the major constituents of leaf EOs were sabinine (14.99%), b-pinene (14.32%), caryophyllene oxide (13.85%), and caryophyllene (9.47%), whereas the rhizome EOs confirmed (Z)-ocimene (21.97%), 4-terpineol (18.45%), γ-terpinene (3.86%) and cis-sabinenehydrate (3%). These EOs could be a promising source for the development of nutraceuticals, functional foods, and pharmaceutical products due to their potent activities and exploitation. Bangladesh J. Bot. 53(1): 153-158, 2024 (March)

Technological aspects of developing drugs in the form of transmucosal and dispersible films

The creation of new forms of drugs is one of the most important areas of development of the modern pharmaceutical industry. Orodispersible films have a number of advantages over conventional tablets and injections. Developing and scaling technology is time-consuming, resource-intensive, and expensive. The determination of critical parameters affecting the technology and quality of the finished product minimizes the risks of obtaining negative results during implementation and routine industrial production. The work presents the studies of the influence of excipients in the formulation on the characteristics of the dosage form and the technological process. The approach to development was considered from the standpoint of choosing a qualitative composition of the drug. The experiments were aimed at determining the most suitable viscosity of hydroxypropyl methylcellulose (HPMC), which makes up the matrix of the dosage form; optimal polymer- plasticizer ratios have been established. The studies carried out are basic in the creation of a transmucosal dosage form and orodispersible films and make it possible to optimize the process of developing the dosage form and reduce risks during the transfer of technology to production.

Темір-көміртекті қорытпалардағы негізгі элементтермен және металл емес қосындылармен сирек жер металдарының әрекеттесуін зерттеу

The proposed article is devoted to the study of the interaction of rare earth metals with basic elements and non-metallic compounds in iron-carbon alloys. The article discusses the method of modifying iron-carbon alloys with rare-earth metals, the nature and mechanisms of interaction of alloy components. The influence of rare earth metals and non-metallic mixtures on the properties of the material is analyzed. The qualitative composition of nonmetallic inclusions formed in steel grades 09G2FB and 17G1SU modified with yttrium and without the introduction of yttrium-containing material (SIITMISH-1) was studied by the X-ray spectral method. With the formation of complex compounds by the introduction of rare earth metal, as a result of which the alloy is purified from non-metallic compounds. Research in this area makes it possible to develop new materials with improved characteristics and optimize the processes of their production and processing

Study of the spectrum and antibiotic sensitivity of the respiratory tract microbiota of newborns with infectious complications during long-term mechanical lung ventilation

determining the structure of leading microbial colonizers of the respiratory tract as potential causative agents of ventilator-associated pneumonia and their sensitivity to antibacterial drugs plays an important role in choosing effective tactics for etiotropic therapy of newborns. According to the data of scientific publications, the etiological structure of infectious complications associated with long-term respiratory support in newborns is known to have certain features. The spectrum of nosocomial microbial pathogens and their sensitivity to antimicrobial drugs are among them. The patients of early age are characterized by a number of peculiarities of anatomical and physiological characteristics and the qualitative and quantitative composition of the microbiota of the respiratory tract. In order to form ideas about the determining causative agents of VAP in newborns, it was advisable to conduct a microbiological study of the species composition of microorganisms associated with this infectious complication, as well as to determine their sensitivity to antibiotics recommended by the management protocols of the corresponding pathology. To study and solve this problem, it is necessary to study the spectrum and antibiotic sensitivity of the causative agents of VAP. A prospective study was conducted in 69 newborns with VAP (2020 - 2023), who were treated in the Neonatal Intensive Care Unit (NICU) of the Vinnytsia Regional Children's Clinical Hospital (VRCCH) and consisted in studying the composition of the microbiota and antibiotic sensitivity. We took into account opportunistic microorganisms that are not characteristic of this biotope of the respiratory tract of newborns who were diagnosed with VAP during long-term mechanical ventilation. As a result of the prospective analysis of the microbiological study of the microbiota of 69 newborns, 82 microorganisms - etiologically significant pathogens of VAP - were isolated and identified. Thus, according to the data of our bacteriological studies, 17 Gram-positive and 65 Gram-negative pathogens of VAP were isolated and identified in NICU VRCCH for 2020-2023. As a result of the study of antibiotic sensitivity of VAP pathogens in newborns, the following trends were observed. Low sensitivity of Acinetobacter baumannii to ceftazidime, aztreonam, imipinem, meropenem, amoxicillin, piperacillin, levofloxacin, ciprofloxacin was established. And sensitive to amikacin, gentamicin and cefoperazone-sulbactam. High rates of resistance of Pseudomonas aeruginosa to ceftazidime, doxycycline, imipinem, meropenem, gentamicin, piperacillin, levofloxacin, ciprofloxacin, ofloxacin were established. The best indicators of sensitivity of P. aeruginosa to amikacin, cefoperazone-sulbactam and tobramycin were found. According to the results of the sensitivity of K. pneumoniae to antibiotics, resistance to all antibiotics was established. The development of resistance of Staphylococcus aureus to azithromycin, norfloxacin, cefotaxime, benzylpenicillin has been established. The indicators of S. aureus sensitivity to amikacin, tetracycline and vancomycin turned out to be the best. We took into account opportunistic microorganisms that are not typical for this biotype of the airways of newborns who were diagnosed with VAP during long-term mechanical ventilation. The results of the study indicate the need to take into account the data of a microbiological study with the mandatory identification of isolated pathogens, their microbial load at the site of infection, determining their antibiotic sensitivity is of crucial importance in the selection of etiotropic treatment tactics for newborns with VAP.

Amino acid composition of pollen <i>Pinus sylvestris</i> L. and <i>Pinus sibirica</i> Du Tour growing in the Baikal region

The purpose of the study was to determine the qualitative and quantitative amino acid composition of pollen Pinus sylvestris L. and Pinus sibirica Du Tour. Pine pollen was collected in June 2021 at natural sites on the southeastern coast of Lake Baikal. The mass fraction of crude protein was determined by the Kjeldahl method; the protein composition and individual amino acid content were determined by high-performance liquid chromatography with post-column modification of ninhydrin on an LA8080 automatic analyser (Hitachi, Japan). The crude protein content of the pollen was 14.38–15.94%. Pine pollen protein is shown to contain 17 amino acids, including 9 essential amino acids: valine, isoleucine, leucine, threonine, methionine, phenylalanine, lysine, histidine, and arginine. The content of the sum of amino acids was 141.4–156.5 mg/g, including essential amino acids 45.9-48.4%. The following amino acids are dominant in Pinus sylvestris and Pinus sibirica pollens (mg/g): monoaminodicarboxylic acids – glutamic (21.3–24.2) and aspartic (13.0–14.2), diaminocarboxylic acid arginine (17.0–17.4) and heterocyclic amino acid proline (14.7–16.2). The obtained results can be useful in the development of drugs and biologically active additives based on pollen Pinus sylvestris and Pinus sibirica, which, due to the presence of the above amino acids, have a nootropic, immunomodulatory, cardiac stimulating, and detoxifying effect.

Studying anthocyanins in the skin and flesh of the tubers of some potato hybrids (<i>Solanum tuberosum</i> L.)

Relevance. Today special attention is paid to potato anthocyanins as components of functional food. Increasing interest is generated by research on the use of anthocyanins for the benefit of animals and people in addition to their significant role in the life of plants. The research goal was to identify the quantitative and qualitative composition of anthocyanins in the tubers of valuable potato hybrids of Primorsky selection. Five promising potato hybrids (Solanum tuberosum L.) belonging to different groups of maturity and involved in the comparative variety testing by FSBSI “FSC of Agricultural Biotechnology named after A. K. Chaiki” were used as the research object. The research was carried out under the conditions of field and laboratory experiments in Primorsky kray, Russia, in 2018-2023. The anthocyanins were divided by the method of high-performance liquid chromatography. The anthocyanins were identified by the method of second-order mass spectrometry. Results. The research evaluated the potato hybrids for the content of anthocyanins in the tissues of the tubers – skin and flesh. The following specimens were determined to have a high content of anthocyanins both in the skin and flesh: Pri-15-12-14 (petunidin-3-arabinoside: 310.0 and 91.1 mg/kg; cyanidin-3-glucoside: 149.8 and 39.8 mg/kg) and Pri-14-52-2 (petunidin-3-arabinoside: 360.0 and 143.0 mg/kg; cyanidin-3-glucoside: 80.4 and 81.2 mg/kg). The selected specimens were characterized by purple and blue-purple skin. Genotypes Pri-14-52-2 and Pri-15-12-14 had tuber flesh with purple pigmentation. Hybrids Pri-15-12-14 and Pri-14-52-2 had the highest total content of anthocyanins in the flesh – 130.9 and 224.2 mg/kg, respectively. The conducted research identified eight different anthocyanins in the tubers of the studied potato specimens: delphinidin-3-glucoside, delphinidin-3-ramnosil-5-glucoside, petunidin-3-glucoside, malvidin-3-glucoside, cyanidin-3-glucoside, cyanidin-3-ramnosil-5-glucoside, pelargonidin-3-glucoside, and petunidin-3-arabinoside. The tubers with pink and red skin were determined to contain pelargonidin-3-glucoside. Petunidin-3-glucoside and cyanidin-3-glucoside colored tuber skin purple. The selected group of specimens might be recommended for use as functional food and in goal-oriented breeding.

Effect of antitumor therapy on microflora and oral mucosa in patients with malignant neoplasms

The present article investigates the relationship between the microbiota of the oral cavity and oral mucositis induced by systemic antitumor therapy in patients with malignant neoplasms. This article highlights modern ideas about the composition of the normal microbiota of the oral cavity and its changes during chemotherapeutic treatment.
 It has been shown that the normal oral microbiota includes representatives of such genera as Actinomyces, Corynebacterium, Fusobacterium, Leptotrichia, Neisseria, Prevotella, Streptococcus, and Veillonella. Moreover, it is emphasized that despite the existence of modern molecular genetic techniques and development of an oral microbiota database, determining the role of individual taxonomic units in oral homeostasis remains challenging.
 Existing studies of changes in the qualitative and quantitative composition of oral microflora against the background of drug antitumor therapy have demonstrated that treatment is associated with significant changes in the microbiological landscape of the oral cavity. An increase was noted in the number of Gram-negative anaerobic opportunistic flora and a decrease in the representation of protective commensal flora. It has been demonstrated that the components of a bacterial cell can modulate the local reactions of a macroorganism through a system of Toll-like receptors while acting in different directions.
 Several unresolved fundamental issues related to the role of oral microbiota in the pathogenesis of oral mucositis are highlighted.

Insight into the influence of natural deep eutectic solvents on the extraction of phenolic compounds from poplar type propolis: Composition and in vitro biological activity

Natural deep eutectic solvents (NADESs) have been considered promising to replace traditional volatile and toxic organic solvents for the extraction of biologically active substances from natural sources. This work applied an efficient and ethanol-exclusion strategy for extraction of phenolic compounds from poplar type propolis using five known NADESs (lactic acid:1,2-propanediol 1:1, lactic acid:fructose 5:1, choline chloride:1,2-propanediol 1:3, choline chloride:1,2-propanediol:water 1:1:1 and betaine:malic acid:water 1:1:6). The selected NADESs' extractability was evaluated by measuring the concentrations of total phenolics and total flavones and flavonols in the propolis extracts obtained, which qualitative chemical composition was further determined in detail by gas chromatography-mass spectrometry (GC-MS) analysis. It demonstrated that the chemical profiles of NADES and 70% ethanolic propolis extracts are similar. To expand the knowledge about the role of the applied solvents in the poplar propolis extraction process, the in vitro antimicrobial, cytotoxic and genotoxic activity of both NADESs and liquid NADES extracts were evaluated. The results revealed that the use of the selected NADESs as an extraction media for phenolic compounds from poplar propolis not only delivered a good extraction yield in some cases, but generally led to the preservation of propolis extracts' biological activity and even to the enhancement of their antimicrobial effect in comparison with the hydroethanolic one. Besides, the tested NADESs except for lactic acid:fructose and betaine:malic acid:water exerted low to negligible toxicity against normal cells treated and apart from lactic acid:fructose the remaining solvents demonstrated concentration-dependent moderate to subtle genotoxicity. There is a probability that not the supramolecular structure of the NADESs, but their components, played a key role for the observed biological effects. The present study has demonstrated an alternative approach for extracting the biologically active complex from poplar type propolis using NADESs, which could be useful for further pharmaceutical and cosmeceutical applications.