Microbiological indicators of cottage cheese using different rennet leavens

Abstract

Rennet cheeses occupy an important place in the diets of the population of Ukraine. The technology of cheese production depends both on the quality of raw materials and on the quality of the enzymes used to curdle milk. Therefore, the study of the effect of rennet enzymes obtained by improved biotechnology on the microbial state of the finished product is of scientific and practical importance. III groups of samples (n = 3) were formed to conduct the experiment. Cow's milk for research was collected from clinically healthy cows during the calving period. In the control group of samples, rennet enzyme of microbial origin was used for curdling milk. In the 1st experimental group of samples, an enzyme preparation was used, extracted from the rennet of dairy calves according to the method of Yu. Ya. Svyridenko In the II experimental group, an enzyme preparation obtained by the method of extraction according to the method of was used. S. V. Merzlova. Laboratory research was carried out in the conditions of the Research Institute of Food Technologies and Technologies of Processing Livestock Products and the Laboratory of Microbiological Research Methods of the Department of Microbiology of the Bilotserk National Agrarian University, which is certified according to (DSTU ISO 10012:2005). To determine the qualitative and quantitative microbial composition, culture was carried out from cheeses obtained with the help of various enzyme preparations. Microbiological research was carried out using the methods defined by DSaNPiN 4.4.5-078-2001 “Microbiological standards and methods of control of food products”. To determine bacterial insemination, two nutrient media such as Lees agar and Streptococcus thermophilus agar were used. The method of serial dilutions was used for seeding the suspension. Sowing was carried out by applying 1.0 cm³ of suspension (dilutions from 1 to 10-5) in melted and cooled agar in Petri dishes, followed by thermostating for 48 hours at 37 °.