Abstract
A deep phycomycotic infection caused by Entomophthora Fres. was reported for the first time in the United States by Gilbert et al. (1970) and was further confirmed by culture and histologic examination of biopsy material by Eckert et al. (1972). The culture was isolated in this case from a hard mass invading the pericardium and the roots of both lungs in a 15-monthold boy and was deposited in the American Type Culture Collection as Entomophthora sp. ATCC 24293. In addition to species of Entomophthora, several species of Conidiobolus Bref. have been isolated from insects and mites (King, 1974). However, the only species of the Entomophthoraceae heretofore reported to be a mammalian pathogen is Conidiobolus coronatus (Cost.) Batko (Bridges et al., 1962; Clark, 1968; Martinson, 1971; Roy and Cameron, 1972). Recently King (1974; 1976) developed methods for the identification of and a key to species of the genus Conidiobolus. Following these procedures, ATCC 24293 was grown on ATCC medium 336 potato dextrose agar (see ATCC Catalogue of Strains, 1974, for formula) in Petri dishes for morphological observations. Cultures were directly examined microscopically and water mounts from cultures were prepared at intervals up to 14 da. Measurements of zygospores and width of older hyphal segments were made from water mounts. In addition, young cultures were inverted over microscope slides to collect primary conidia for measurements in water mounts. Cultures were also inverted over 2% water agar in Petri dishes to allow deposition of conidia for observation of production of types of conidia other than the primary and globose replicative conidia common to all species, and measurement of those conidia. 181
Published Version
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