Protected Plants Research Articles

Combined analysis of metagenome and transcriptome revealed the adaptive mechanism of different golden Camellia species in karst regions

Camellia sect. Chrysantha is an important rare and protected plant species. Some golden Camellia species grow in karst soil while others grow in acidic soil. In order to study the adaptation mechanism of golden Camellia to the karst environment, four species of golden Camellia growing in the karst soil (Camellia pubipetala, Camellia perpetua, Camellia grandis, and Camellia limonia) and four species growing in the acidic soil (Camellia nitidissima, Camellia euphlebia, Camellia tunghinensis, and Camellia parvipetala) were selected for this study. Combining the metagenome and transcriptome, the structure and function of the rhizosphere microbial communities and the gene expression in roots of golden Camellia were analyzed. The results showed that the rhizosphere microbial communities in different golden Camellia were significantly different in abundance of Acidobacteria, Actinobacteria, Candidatus_Rokubacteria, Nitrospirae, Planctomycetes, and Candidatus_Tectomicrobia. The proportion of Candidatus_Rokubacteria was significantly higher in the rhizosphere soil of four species of golden Camellia grown in karst areas, compared to C. nitidissima, C. euphlebia, and C. tunghinensis. The linear discriminant analysis Effect Size showed that C. parvipetala was similar to karst species in the enrichment of ABC transporters and quorum sensing. During the transcriptome analysis, numerous upregulated genes in four karst species, including CYP81E, CHS, F3H, C12RT1, NAS, and CAD, were found to be enriched in the secondary metabolite synthesis pathway in the KEGG library, when compared to C. tunghinensis. This study provides information for plant adaptation mechanisms on the rhizosphere soil microbial composition and gene expression in secondary metabolic pathways to karst habitats and its distribution in karst areas.

Complete chloroplast genome and phylogenetic analysis of Meliosma oldhamii Miq. ex Maxim. (Sabiaceae)

Meliosma oldhamii Miq. is a deciduous arbor and a member of Sabiaceae. It is one of the rare and protected plants with outstanding ornamental and economic value in Jiangsu, China. In addition to the resource values indicated above, there are numerous other elements that require additional research, including the chloroplast (cp) genomic information. In this work, the complete cp genome sequence of M. oldhamii was assembled and characterized for the first time. The complete cp genome of M. oldhamii was 159,950 base pair (bp) in length, including a large single-copy (LSC) region of 87,147 bp, a small single-copy (SSC) region of 18,015 bp, and the inverted repeats (IRs) region of 27,394 bp. It contains 131 genes, including 37 tRNA genes, eight rRNA genes, 85 protein-coding genes, and one pseudogene. The overall GC content of M. oldhamii cp genome was 37.95%. The placement of M. oldhamii in the phylogenetic tree constructed using the complete cp genome is largely congruent with previous studies. The clustering of Meliosma is non-monophyletic, aligns more closely with existing morphological taxonomic studies, thereby enhancing the scholarly and scientific nature of this research.

Seaweed polysaccharides on seed germination of Brassica napus L.

Seaweed polysaccharides, when compared to synthetic commercial fertilizers, have been proven to achieve excellent results in plant growth parameters. When applied to the soil directly or sprayed on the foliage, seaweed poly- and oligosaccharides can improve seed germination and plant vigor, increase the uptake of soil nutrients, and protect plants against several abiotic and biotic stresses, by stimulating a plant to produce secondary metabolites and manage its defense pathways. In this study, three different polysaccharides (alginate, agar and carrageenan) were extracted from one brown seaweed, Saccorhiza polyschides, and two red seaweeds, Gracilaria gracilis and Chondrus crispus, respectively, with aim to analyse their impact on Brassica napus L. seed germination. The polysaccharides' chemical structure, mineral profile and other physicochemical properties were assessed. Carrageenan exhibited the best results in seedling growth and germination percentage when compared to alginate and agar. The carrageenan extracted from the tetrasporophyte, non-fructified thalli and female gametophyte of Chondrus crispus exhibited 94.67, 92 and 98.67 % seed germination, respectively. Carrageenan's bioactivities appear to be related with their sulphation level, plus the pH and the conductivity of its solution. The optimum concentration for germination of each polysaccharides' solution was assigned to propose further experiments in turnip greens.

γ-Aminobutyric acid (GABA) and ectoine (ECT) impacts with and without AMF on antioxidants, gas exchange attributes and nutrients of cotton cultivated in salt affected soil

Salinity stress is one of the major hurdles in agriculture which adversely affects crop production. It can cause osmotic imbalance, ion toxicity that disrupts essential nutrient balance, impaired nutrient uptake, stunted growth, increased oxidative stress, altered metabolism, and diminished crop yield and quality. However, foliar application of osmoprotectant is becoming popular to resolve this issue in crops. These osmoprotectants regulate the cellular osmotic balance and protect plants from the detrimental effects of high salt concentrations. Furthermore, the role of arbuscular mycorrhizae (AMF) is also established in this regard. These AMF effectively reduce the salinity negative effects by improving the essential nutrient balance via the promotion of root growth. That’s why keeping in mind the effectiveness of osmoprotectants current study was conducted on cotton. Total of six levels of γ-Aminobutyric acid (GABA = 0 mM, 0. 5 mM, and 1 mM) and ectoine (ECT = 0 mM, 0.25 mM, and 0.5 mM) were applied as treatments in 3 replications. Results showed that 0.5 mM γ-Aminobutyric acid and ectoine performed significantly best for the improvement in cotton growth attributes. It also caused significant enhancement in K and Ca contents of the leaf, stem, bur, and seeds compared to the control. Furthermore, 0.5 mM γ-Aminobutyric acid and ectoine also caused a significant decline in Cl and Na contents of leaf, stem, bur, and seeds of cotton compared to control under salinity stress. A significant enhancement in chlorophyll contents, gas exchange attributes, and decline in electrolyte leakage validated the effectiveness of 0.5 mM γ-Aminobutyric acid and ectoine over control. In conclusion, 0.5 mM γ-Aminobutyric acid and ectoine have the potential to mitigate the salinity stress in cotton.

Combined application of Pseudomonas fluorescens and urea can mitigate rapid acidification of cropland Ultisol

Rhizobacteria maintain a healthy soil required for crop growth. This has led to increased interest in the use of bacteria-based biofertilizers in agriculture as they improve soil nutrient content and protect plants against pathogens. However, the effect of bacteria inoculum on N transformation and soil physicochemical properties during urea fertilization remains unexploited. Thus, this study investigated the effect of Pseudomonas fluorescens on urea N transformation in an acidic Ultisol within a 70-d incubation period. The results revealed that (1) soil pH peaked on d 5 (pH 5.58) and 20 (pH 6.23) and rapidly decreased till d 62 (pH 4.10) and 50 (pH 4.93) for urea and urea + bacteria treatments, respectively, and remained constant thereafter. After 70 d, the pH of the bacteria-treated Ultisol remained higher (0.78 pH units) than that of urea-treated Ultisol; (2) the change in soil pH was in agreement with the mineralization trend of N, as the concentration of NH4+-N peaked on d 5 (134.2 mg N kg−1) and 20 (423 mg N kg−1) before decreasing to 62.1 and 276.1 mg N kg−1 on d 70 in urea-treated and bacteria-treated Ultisol, respectively; and (3) P. fluorescens consumed protons produced during nitrification to retard rapid decrease in soil pH, decreased soil exchangeable acidity (33.3 %), increased soil effective cation exchange capacity (32.8 %), and increased the solubility of soil exchangeable base cations (68.4 %, Ca2+ + Mg2+ + K+ + Na+). Thus, bacterial inoculum could promote N mineralization, enhance nutrient solubility, and retard soil acidification during N transformation in soils.

Effects of Seed Biological Characteristics and Environmental Factors on Seed Germination of the Critically Endangered Species Hopea chinensis (Merr.) Hand.-Mazz. in China

Hopea chinensis (Merr.) Hand.-Mazz. is a Class II national key protected plant and Plant Species with Extremely Small Populations in China. In order to further analyze why H. chinensis is endangered and optimize conservation techniques, we carried out a study on the effects of seed biological characteristics and environmental factors on the seed germination and seedling growth of H. chinensis. The results show that there were no significant differences in seed morphology between four populations in southern China, but there were significant differences in calyx lobe morphology and seed germination. The removal or retention of the calyx lobes or the seed coat had no significant effect on seed germination. The weight of individual H. chinensis seeds was mainly >1.0 g, with small seeds (<0.5 g) not germinating, whereas seeds >2.0 g had the highest germination rate and fastest seedling growth. H. chinensis produces typical recalcitrant seeds, being sensitive to natural dehydration, with dehydration for 8 d reducing seed viability by 50%, whereas dehydration for 16 d resulted in seeds with zero viability. H. chinensis seeds are light-neutral and capable of germination at a temperature of 30 °C and on a substrate with good water retention and aeration. The seeds are not tolerant of drought, salinity, or flooding. In conclusion, the unique biological characteristics of H. chinensis seeds and the specific habitat which they inhabit contribute to a significant loss of viable seeds, which negatively affects the population’s ability to regenerate and achieve maintenance.

Effect of Pesticide Exposure on Women Farmers of Reproductive Age on Growth and Development Disorders of Children Born: A Systematic Literature Review

The large number of children suffering from ADHD and autism today is a result of the increasing use of pesticides in agriculture. This applies to women farmers of childbearing age who work in agricultural settings or women whose work is related to the use of pesticides. Pesticides are a mixture of several chemicals used to control pests and protect plants. With the use of pesticides, yields obtained increased significantly. This study aims to examine the effect of exposure to pesticides on female farmers of childbearing age on growth and development disorders of children born. The review was conducted according to state-of-the-art methods using selected reporting item guidelines for reviews and meta-analyses (PRISMA). We reviewed the literature from several publications and analyzed the effect of exposure to pesticides on female farmers of childbearing age on developmental disorders of the children they were born with. The results of this study are the effect of exposure to pesticides on female farmers of childbearing age on birth defects. The use of pesticides causes pesticide poisoning. Accumulation of pesticides at a certain threshold has a negative impact on the body, namely brain disorders, tumors, cancer, even in pregnant women can cause birth defects. Apart from adults, pesticides also have a negative impact on children

Natural Regeneration of Forest Stands after Ground Fires in the Rostov Region

Forest fires cause significant damage to protective plantations in the Rostov region. The largest area of forest fires in the steppe zone is in areas where the predominant species of protective plantations are Crimean pine or Scots pine. According to the adapted classification of fireplaces, the forest stands affected by ground fires are evaluated as heavily damaged. Despite an increase in the area of light, the projective cover of herbaceous vegetation reaches 80–100% only in the 4th-5th year after the fire. Natural regeneration of protective plantations occurs at the expense of invasive species of woody vegetation, such as maple ash, small-leaved elm, and green ash. In order to study the rate and extent of natural regeneration of the stand after ground fires, an assessment of plantations in the Shakhtinsky forest area of the Rostov region that were affected by ground fires in 2018–2022 was carried out.

Effects of Different Salinity Levels in Drip Irrigation with Brackish Water on Soil Water-Salt Transport and Yield of Protected Tomato (Solanum lycopersicum)

The effective exploration and utilization of brackish water resources are crucial to alleviating the scarcity of freshwater in arid regions. This study focused on protected tomato plants and set up four irrigation salinity levels: T1 (2 g·L−1), T2 (4 g·L−1), T3 (6 g·L−1), and T4 (8 g·L−1), with freshwater irrigation as a control (CK). The aim was to investigate the effects of continuous brackish water irrigation on soil water-salt transport and tomato yield. The outcomes highlighted that the moisture content in different layers of soil exhibited a “high in the middle, low at both ends” pattern, with the primary accumulation of soil moisture occurring at the 40 cm depth. The range and moisture content of the soil wetted zone increased with elevated salinity levels. Under continuous brackish water irrigation, the range of the soil wetted zone expanded further for the autumn crops, and the moisture content significantly increased compared to the spring crops. The concentration of soil salt gradually decreased with increasing soil depth, exhibiting greater levels in the 0–20 cm layer compared to the 40–80 cm layer. The average salt concentration in the soil at the end of the growth period was significantly higher than before transplantation, and this phenomenon became more pronounced with increasing salinity levels. Initial irrigation with brackish water with a salinity level of 2–4 g·L−1 promoted the growth of the tomatoes planted in the spring and the plant height and stem diameter reached the peak values of 1.68 m and 1.08 mm for the T2 treatment, respectively, which were 7.1% and 9.2% higher than that of the CK treatment, ensuring efficient yield and water usage. However, continuous irrigation with brackish water with a salinity level of 2–4 g·L−1 inhibited the growth and yield of the tomatoes planted in autumn, while the T1 and T2 treatments only yielded 24,427.42 and 16,774.86 kg·hm−2, respectively, showing a decline of 32.2% and 46.1% compared to the yields of the spring season. Considering the soil water-salt and yield indicators, under the conditions of non-continuous brackish water irrigation, using water with a salinity level of 2–4 g·L−1 is recommended for drip irrigation of protected tomatoes.

Isolation And Identification of Arbuscula Mycorrhiza (Ma) In Plant Rhizosphere in Greenbelt Area of PT. Semen Indonesia Tbk Tuban

<p>The Green Belt area is a buffer area in an industrial area that functions as a barrier or separates the mining area from other regions, such as residential areas or community agriculture. The Green belt area consists of various plant vegetation that absorbs carbon dioxide and air conditioning, preventing sedimentation and erosion in the mining area. Arbuscular mycorrhiza has the potential to live in the green belt area by forming a mutualism symbiosis with plants or vegetation in the area. This study aimed to determine arbuscular mycorrhizae found in the Greenbelt area of PT Semen Indonesia Tbk Tuban. Sampling used purposive sampling techniques at three sampling stations, namely rhizosphere eucalyptus (<em>Malaleuca cajuputi</em>), trembesi (<em>Samanea saman),</em> and sawo (<em>Manilkara zapota</em>). The study was conducted in November 2021 through three stages: sampling, sample isolation, and species identification. The results showed seven types of arbuscular mycorrhiza from five different genera, namely the genus <em>Glomus</em>, <em>Acaulospora</em>, Gigaspora, <em>Racocetra</em>, and <em>Archaeospora</em>. The most found species is the genus <em>Glomus</em>. This research contributes as a source of literacy and information about the types of AMF found in PT Semen Indonesia's Greenbelt area, which can be used as biological fertilizer, support plant growth, help reclaim mining areas, and protect plants from drought pressure in mining areas.</p>

Coupling of different antioxidative systems in rice under the simultaneous influence of selenium and cadmium

Selenium (Se) elevates the antioxidant ability of rice against cadmium (Cd) stress, but previous studies only focused on the variation in antioxidant enzymes or nonenzymatic substances induced by Se under Cd stress and ignored the relationships between different antioxidant parameters during the interaction. Here, hydroponic experiments with rice were performed by adding both Cd and Se at doses in the range of 0–50 μM to explore the physiological responses of rice and their relationships in the presence of different levels of Se and Cd. Exogenous Cd markedly promoted the activity of antioxidant enzymes with the exception of catalase (CAT) and the concentration of nonenzymatic substances in aerial parts. Se enhanced the antioxidant capacity by improving the activities of all the enzymes tested in this study and increasing the concentrations of nonenzymatic compounds. The couplings among different antioxidant substances within paddy rice were then determined based on cluster and linear fitting results and their metabolic process and physiological functions. The findings specifically highlight that couplings among the ascorbic acid (AsA)-glutathione (GSH) cycle, glutathione synthase (GS)-phytochelatin synthetase (PCS) coupling system and glutathione peroxidase (GPX)-superoxide dismutase (SOD) coupling system in aerial parts helps protect plants from Cd stress. These coupling systems form likely due to the fact that one enzyme generated a product that could be the substrate for another enzyme. Noticeably, such coupling systems do not emerge in roots because the stronger damage to roots than other organs activates the ascorbate peroxidase (APX)-GPX-CAT and PCS-GS-SOD systems with distinct functions and structures. This study provides new insights into the detoxification mechanisms of rice caused by the combined effect of Se and Cd.

Rhizosphere Microorganisms with Different Strategies and Mechanisms to Enhance Plant Growth in the Occurrence of Different Environmental Stress Factors

Every minute, the world’s population grows, and in order to feed them, crop output and agricultural productivity must be improved by adding crucial microorganisms that boost plant yields in various ways through nitrogen fixation, the secretion of both plant growth regulators and 1-aminocyclopropane 1-carboxylate deaminase, as well as some antimicrobial agents. Numerous endophytic bacteria have recently been used to increase plant yields, and agricultural production in addition to reducing salt stresses. Many scientists have made an effort to clarify and comprehend the processes by which bacteria promote plant growth and production. A vital substance known as 1-aminocyclopropane-1-carboxylate (ACC) deaminase is produced by several bacteria, plants, and fungi to decrease ethylene levels in a plant grown under different environmental stress. The gaseous hormone ethylene (C2H4) is synthesized in plant tissues from the precursor ACC, and it has numerous biochemical roles in plants, such as cells differentiation and tissue development, seedling, root hair, leaf, and flower growth and development in addition to fruit ripening and formation of anthocyanin and volatile compounds. Thus, this critical enzyme had influential roles in plants during their positive interaction with bacteria which increase plant growth due to auxin production and protect plants against different environmental stress like drought, high salts, wilting, high level of heavy metals, contaminants with pesticides, and microbial pathogen infections. Different bacterial genera are highly ACC deaminase-producer, and these bacteria support plant growth and agricultural process. In conclusion, bacteria can replace chemicals in a variety of environmentally benign methods to boost soil fertility and plant productivity. However, much research is required to determine the efficacy of these bacteria before suggesting their use on a broad scale in the field.

Environmental impact screening for urban land use transitions - a perspective from developing a hill land development in Penang Island and shaping community acceptance

The current rate of urbanisation is unprecedented in human history, particularly in Malaysia’s densely populated areas. Natural constraints and hazards are frequently ignored in this informal and sprawling urban growth, exposing the population to these hazards. Deforestation, water catchment loss, extinction of protected species and plants, soil erosion, landslides, water pollution, sedimentation, and downstream flooding have all resulted from inadequate environmental mitigation. Hence, this research quantifies and assesses the environmental and social impacts of hill land development on the affected areas. The community’s opinion on such growth and environmental mitigation measures will be assessed and evaluated at a study site on Penang Island, Malaysia. Environmental Impact Screening (EIS) was conducted by distributing questionnaires to affected residents and businesses, with 69 respondents. Descriptive statistical analysis was performed using the Statistical Package (SPSS), followed by a thorough elaboration on the pros and cons of expanding this steep land by considering social, economic, and environmental consequences. From the evaluation, most respondents were opposed to hill land development that can harmed community’s ecosystem. As a result, numerous efforts have been made to reduce community and environmental impacts. This study enables stakeholders to assess the environmental impacts from this type of developments and conduct necessary steps to mitigate or eliminate them.

Beauvericin Immunotoxicity Prevention by Gentiana lutea L. Flower In Vitro.

Beauvericin (BEA) is an emerging mycotoxin produced by some species of Fusarium genera that widely contaminates food and feed. Gentiana lutea is a protected medicinal plant known for its antioxidant and anti-inflammatory properties, which are attributed to its rich content of bioactive compounds. In order to evaluate the beneficial effects of G. lutea flower against BEA cytotoxicity, the aim of this study is to evaluate changes in protein expression after Jurkat cell exposure through a proteomics approach. To carry out the experiment, cells were exposed to intestinally digested G. lutea flower alone or in combination with the BEA standard (100 nM) over 7 days. Differentially expressed proteins were statistically evaluated (p < 0.05), revealing a total of 172 proteins with respect to the control in cells exposed to the BEA standard, 145 proteins for G. lutea alone, and 139 proteins when exposing the cells to the combined exposure. Bioinformatic analysis revealed processes implicated in mitochondria, ATP-related activity, and RNA binding. After careful analysis of differentially expressed proteins, it was evident that G. lutea attenuated, in most cases, the negative effects of BEA. Furthermore, it decreased the presence of major oncoproteins involved in the modulation of immune function.

Impact of Agrochemicals on Beneficial Microorganisms and Human Health

Agrochemicals, including fertilizers, pesticides, and herbicides, are widely used in agriculture to improve crop yields and protect plants from pests and diseases. There is growing concern over their impact on beneficial soil microorganisms and, indirectly, on human health. This review aims to provide an in-depth analysis of the effects of agrochemicals on soil microbial communities and human health, focusing on recent scientific research and case studies. Exploring various agrochemicals can disrupt microbial diversity, population, and functionality, affecting crucial soil processes and, in turn, ecosystem health. We delve into the pathways of human exposure to agrochemicals and the potential health implications. To mitigate the adverse effects of agrochemicals, the review highlights several alternative approaches, including the use of organic fertilizers and pesticides, precision agriculture, and genetically modified crops. Despite these advancements, research gaps persist in understanding the complex interplay between agrochemicals, beneficial microorganisms, and human health, particularly in the changing agricultural practices and climate conditions. We argue that interdisciplinary, long-term studies are needed to fill these gaps and develop sustainable, health-conscious agricultural practices. The review is intended for researchers, policymakers, and agricultural practitioners seeking to understand and address the environmental and health impacts of agrochemicals.

Recent insights into pesticide resistance mechanisms in Plutella xylostella and possible management strategies.

Insects are incredibly successful and diverse organisms, but they also pose a significant threat to agricultural crops, causing potential losses of up to US$470 billion. Among these pests, Plutella xylostella (Linnaeus), a devastating insect that attacks cruciferous vegetables, alone results in monetary losses of around US$4-5 billion worldwide. While insecticides have effectively protected plants under field conditions, their use comes with various environmental and mammalian hazards. Additionally, insects are developing resistance to commonly used insecticides, rendering management strategies less effective. Arthropods employ a range of behavioral and biochemical mechanisms to cope with harmful chemicals, which contribute to the development of resistance. Understanding these mechanisms is crucial for addressing the issue of resistance. It is imperative to integrate strategies that can delay the development of resistance and enhance the efficiency of insecticides. Therefore, we present an overview of insecticide resistance in insects, focusing on P. xylostella, to provide insights into the current resistance status of this pest and propose tactics that can improve the effectiveness of insecticides.

Endosperm Persistence in Arabidopsis Results in Seed Coat Fractures and Loss of Seed Longevity.

Seeds are specialized plant organs that carry, nurture, and protect plant offspring. Developmental coordination between the three genetically distinct seed tissues (the embryo, endosperm, and seed coat) is crucial for seed viability. In this study, we explore the relationship between the TFs AtHB25 and ICE1. Previous results identified ICE1 as a target gene of AtHB25. In seeds, a lack of ICE1 (ice1-2) suppresses the enhanced seed longevity and impermeability of the overexpressing mutant athb25-1D, but surprisingly, seed coat lipid polyester deposition is not affected, as shown by the double-mutant athb25-1D ice1-2 seeds. zou-4, another mutant lacking the transcriptional program for proper endosperm maturation and for which the endosperm persists, also presents a high sensitivity to seed aging. Analysis of gso1, gso2, and tws1-4 mutants revealed that a loss of embryo cuticle integrity does not underlie the seed-aging sensitivity of ice1-2 and zou-4. However, scanning electron microscopy revealed the presence of multiple fractures in the seed coats of the ice1 and zou mutants. Thus, this study highlights the importance of both seed coat composition and integrity in ensuring longevity and demonstrates that these parameters depend on multiple factors.

The effect of layers of high tunnel covering and soil mulching on tomato fruit quality.

High tunnels (HT) and plastic mulch can increase yield, extend growing seasons, protect plants from harsh weather conditions, and produce more marketable and attractive tomatoes. However, plastic covering reduces solar radiation, which may affect the quality of tomatoes. This study investigated the effects of single-layer versus double-layer HT and plastic mulch versus bare soil on the quality of fully ripe tomato fruits. The study was conducted over 2 years (2021 and 2022). The fruit color (L*, a*, and b*), vitamin C, pH, total titratable acid (TTA), total soluble solids (TSS), and lycopene content of the two tomato cultivars (Brandywine and Rebelski) were quantified. The results showed that quality parameters varied with the plastic layers of HT, plastic mulch, and planting year. The double-layer HT only showed a slight but significant influence on the color of tomatoes of both cultivars in the same year (P < 0.05). Brandywine tomatoes grown in the double-layer HT had significantly lower pH and lycopene content than those grown in single-layer HT, regardless of mulching. The effect of plastic layers on TTA, TSS, and lycopene depended on whether the soil was mulched. Tomatoes grown on bare soil had higher TTA and TSS values than those grown on mulched soil in double-layer HT. Tomatoes grown in single-layer HTs had significantly higher vitamin C content than those in the double-layer HT for both cultivars regardless of mulching. This study demonstrates that double-layer HT is unnecessary for the improvement of the overall quality of tomatoes. © 2023 Society of Chemical Industry.

Predicting dynamic trajectories of a protected plant community under contrasting conservation regimes: Insights from data-based modelling

Data-based modelling of the dynamic behaviour of ecological communities is a big challenge in systems ecology and conservation biology. Implementing such models to forecast future scenarios is key for supporting decision-making in ecological reserves, given the multiple disturbances threatening their future. Using demographic and dynamic data for three tree (or tree-like) species from the Pedregal de San Ángel Ecological Reserve (Mexico City, Mexico), we constructed a family of dynamical models (using systems of ordinary differential equations) to reconstruct their dynamic interactions. The first two models considered two native species (Pittocaulon praecox and Buddleja cordata), with either antagonistic or cooperative interactions between them. These species have been considered ecosystem engineers playing different roles in the system and determining several community attributes. These models predict the transition from a stable steady state dominated by P. praecox (as reported in the 1950s) to the current community whose structure is apparently shifting to an alternative stable state dominated by B. cordata. The second pair of models additionally incorporate the invasive tree Eucalyptus camaldulensis, which exerts negative effects on all native plant species. The prediction of these models is an E. camaldulensis-dominated state with the exclusion of the native species from the reserve, provided this invasive species follows current population growth trends, without external intervention to check this process. Bifurcation analysis of this latter model allowed us to rationally design optimal intervention strategies that could potentially divert the trajectory from converging to an E. camaldulensis-only configuration into the stable coexistence of the native species. For the two-species and three-species models we made two versions, one with only competitive interactions and another one including a facilitation interaction between B. cordata and P. praecox. This analysis shows that facilitation is a requirement to achieve stable coexistence between the two native species, even in the presence of E. camaldulensis. The models constructed here, which integrate multiple data sources, help clarify conflicting empirical information regarding potential ecological mechanisms, and allow making predictions for strengthening the future management and E. camaldulensis control programs before its effects irreversibly modify the functioning and biodiversity of the biotic community protected in the reserve.

Geographical distribution and conservation strategy of national key protected wild plants of China

National key protected wild plants (NKPWPs) are considered flagship species for plant diversity conservation in China. Using data for 1101 species, we characterized NKPWPs distribution patterns in China and assessed conservation effectiveness and conservation gaps. In total, 4880 grid cells at a 20×20km resolution were filled with occurrence records for NKPWPs. We identified 444 hotspot grid cells and 27 diversity hotspot regions, containing 92.37% of NKPWPs. However, 43.24% of these hotspot grid cells were fully or partially covered by national nature reserves (NNRs), where 70.21% of species were distributed. Approximately 61.49% of the NKPWPs species were protected by NNRs, but the populations or habitats of 963 species were partially or fully outside of NNRs. With global warming, the overall change in the extent of suitable habitats for NKPWPs is expected to be small, however, habitat quality in some areas with a high habitat suitability index will decrease.