Combined application of Pseudomonas fluorescens and urea can mitigate rapid acidification of cropland Ultisol

Abstract

Rhizobacteria maintain a healthy soil required for crop growth. This has led to increased interest in the use of bacteria-based biofertilizers in agriculture as they improve soil nutrient content and protect plants against pathogens. However, the effect of bacteria inoculum on N transformation and soil physicochemical properties during urea fertilization remains unexploited. Thus, this study investigated the effect of Pseudomonas fluorescens on urea N transformation in an acidic Ultisol within a 70-d incubation period. The results revealed that (1) soil pH peaked on d 5 (pH 5.58) and 20 (pH 6.23) and rapidly decreased till d 62 (pH 4.10) and 50 (pH 4.93) for urea and urea + bacteria treatments, respectively, and remained constant thereafter. After 70 d, the pH of the bacteria-treated Ultisol remained higher (0.78 pH units) than that of urea-treated Ultisol; (2) the change in soil pH was in agreement with the mineralization trend of N, as the concentration of NH4+-N peaked on d 5 (134.2 mg N kg−1) and 20 (423 mg N kg−1) before decreasing to 62.1 and 276.1 mg N kg−1 on d 70 in urea-treated and bacteria-treated Ultisol, respectively; and (3) P. fluorescens consumed protons produced during nitrification to retard rapid decrease in soil pH, decreased soil exchangeable acidity (33.3 %), increased soil effective cation exchange capacity (32.8 %), and increased the solubility of soil exchangeable base cations (68.4 %, Ca2+ + Mg2+ + K+ + Na+). Thus, bacterial inoculum could promote N mineralization, enhance nutrient solubility, and retard soil acidification during N transformation in soils.