You have accessJournal of UrologyProstate Cancer: Basic Research III1 Apr 2014MP41-12 EXPRESSION OF I BLOOD GROUP ANTIGEN SYNTHASE CORRELATES WITH MALIGNANT POTENTIAL IN PROSTATE CANCER Yuki Tobisawa, Tohru Yoneyama, Hayato Yamamoto, Kazuyuki Mori, Shingo Hatakeyama, Atsushi Imai, Takahiro Yoneyama, Yasuhiro Hashimoto, Takuya Koie, Minoru Fukuda, and Chikara Ohyama Yuki TobisawaYuki Tobisawa More articles by this author , Tohru YoneyamaTohru Yoneyama More articles by this author , Hayato YamamotoHayato Yamamoto More articles by this author , Kazuyuki MoriKazuyuki Mori More articles by this author , Shingo HatakeyamaShingo Hatakeyama More articles by this author , Atsushi ImaiAtsushi Imai More articles by this author , Takahiro YoneyamaTakahiro Yoneyama More articles by this author , Yasuhiro HashimotoYasuhiro Hashimoto More articles by this author , Takuya KoieTakuya Koie More articles by this author , Minoru FukudaMinoru Fukuda More articles by this author , and Chikara OhyamaChikara Ohyama More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.1229AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail Introduction and Objectives To avoid over treatment of localized prostate cancer (PCa), it is very important to develop a novel biomarker for malignant potential of PCa. I blood group antigen synthase (GCNT2) is a glycosyltransferase which forms I-branching N-glycan on various glycoproteins. In case of breast cancer, other group already reported, GCNT2 expression was correlated with cancer migration and invasion (Zhang H, et. al., Cancer Res. 2011). We examined whether immunohistochemical detection of GCNT2 can predict PSA recurrence after radical prostatectomy. Methods To determine the correlation of GCNT2 expression with pathological parameters and biochemical outcome after radical prostatectomy, paraffin embedded specimens of 156 patients with PCa were immunohistochemically examined for GCNT2 expression using the Rabbit anti-GCNT2 polyclonal antibody. Normal prostate epithelial cell line RWPE-1 and PCa cell lines (LNCaP, DU145 and PC-3) were analyzed expression of GCNT2 by quantitative PCR methods. Results GCNT2 positive rate in pT2 (67.4%) was significantly lower than that in pT3/pT4 (82.5%/100%) (chi-square-test, p=0.014). GCNT2-negative patients showed significantly longer PSA free survival than GCNT2-positive patients (log-rank test, p=0.0007). Multivariate analysis revealed initial PSA (iPSA), High risk (DfAmico), margin status (RM) and GCNT2 expressions were independent risk factors for PSA recurrence. PCa cell lines had significantly higher expression of GCNT2 mRNA than RWPE-1. Conclusions These results suggest that I-branching N-glycan is one of the predictor of prostate cancer and immunohistochemical detection of GCNT2 of radical prostatectomy specimen is an independent and strong indicator of PSA recurrence after radical prostatectomy. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e454-e455 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Yuki Tobisawa More articles by this author Tohru Yoneyama More articles by this author Hayato Yamamoto More articles by this author Kazuyuki Mori More articles by this author Shingo Hatakeyama More articles by this author Atsushi Imai More articles by this author Takahiro Yoneyama More articles by this author Yasuhiro Hashimoto More articles by this author Takuya Koie More articles by this author Minoru Fukuda More articles by this author Chikara Ohyama More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...