ABSTRACT Health communication affects people’s decision-making that promotes health. Well-designed health communication materials have the potential to impact social norms and behaviors and lead to a healthier life. This study assessed health communication materials use among health professionals working at health-care institutions in Gondar city, Northwest Ethiopia. A health facility-based cross-sectional study was conducted. A self-administered questionnaire was used for data collection. Descriptive statistics were computed, and logistic regression analysis was fitted to identify factors associated with health communication materials use. Adjusted odds ratio (AOR) with a 95% confidence interval (CI) and p-value <0.05 were used to measure the strength of the association and declare significant variables, respectively. A total of 298 health professionals participated in the study with a response rate of 96.40%. Approximately two-thirds (67.50%) of the study participants ever used health communication materials. Being a midwifery (AOR = 0.31; 95% CI: 0.11, 0.85) and laboratory (AOR = 0.31; 95% CI: 0.10, 0.93) professional, and not delivering health education (AOR = 0.09, 95% CI: 0.04, 0.21), were negatively associated with use of health communication materials. On the other hand, working at a health center (AOR = 3.60, 95% CI: 1.52, 8.54), availability of the materials (AOR = 2.23, 95% CI: 1.10, 4.53) and having good knowledge about the materials (AOR = 2.69, 95% CI: 1.36, 5.32) were positively associated with health communication materials use. Health professionals’ health communication materials use is critical in promoting pro-health attitudes and behaviors. Improving the availability of health communication materials, harmonizing health communication activities, and building the capacity of health professionals need to be emphasized.