Corn Production Research Articles

AGRICULTURE TRANSFORMATION AND AGRIBUSINESS DEVELOPMENT IN TRANSITION COUNTRIES: THE CASE OF POLOG REGION IN NORTH MACEDONIA

The belief in different circles that agriculture is dealt with mainly by the poorer countries, and that the development of other branches of the economy such as industry can push it forward in itself, to some extent has overshadowed the strategic role of this sector, especially in primary production or agricultural products. The sharp rise in prices of corn and derivative products in 2010 as a result of draught, began to alarm even the consumers with higher incomes who should spent more on food consumption. Undoubtedly, for developing countries that do not satisfy their own needs with food and depend on importing, agriculture development requires a more serious and decisive commitment than ever before. While this necessity cannot be questioned by anyone, this paper aims to analyze agriculture reform challenges in North Macedonia, where agriculture contributes 10 percent to GDP and 18 percent to employment, and based on survey data of eight agribusiness companies from the Polog region, to identify the development prospects of this sector.

Advancements in breeding for high oil content in maize (Zea mays. L)

Maize is one of the world’s most important food crops, supplying>5% deity energy. Due to its wider adaptability, high yield potential, maize is widely utilized as food and animals world worldwide. It enriched with caloric starch and the high energy in its oil. The kernel oil content in commercial high-oil maize hybrids averages ∼8%, far lower than that in developed high-oil maize lines (as high as 20%). The maize seed/kernel consisted of endosperm (82%) and the germ (embryo and scutellum) (12%). The germ region makes up 80–84% of kernel oil, with the aleurone and endosperm making up 12% and 5%, respectively. High-oil maize may serve as a valuable forage maize germplasm for breeding. Maize oil is also considered as high-quality oil for human health due to the high proportion of polyun-saturated fatty acids. Selection for high oil increases the proportion of germ and further content of germ oil. The problems that over-shadow the successful production of high-oil corn are low grain yield potential, physiological cost of oil synthesis, low seed vigor, low kernel weight, shorter seed longevity, and poor germination of high-oil corn lines. It is necessary to make efforts to generate nested populations or use marker-assisted recurrent selection (MARS) to accumulate the advantageous quantitative trait loci (QTLs) in the pa-rental lines. The oil content of maize inbreeds and hybrids could be further improved with the use of transgenes, genomics, and metabolic engineering technologies in new insight for the development of high oil maize breeding and strategies.

Growth and nitrogen uptake modeling in composite and hybrid corn varieties

Corn is a strategic commodity in Indonesia's agricultural and economic development. The problem of corn production can be overcome with proper usage of nitrogen fertilization. Dynamic modeling can be used as a tool to solve this problem. This study aimed to develop a dynamic model for the growth and nitrogen uptake of composite and hybrid corn varieties. Stages in the model construction: (1) identifying system components, (2) model construction, (3) simulation, and (4) validation. The growth model is a construction of the plant's ecophysiological response to solar radiation and temperature in photosynthesis. A growth model in composite and hybrid corn varieties with simulated dry weight as output has been constructed validly. The model input components, such as light use efficiency, extinction coefficient, specific leaf area, and carbohydrate partition coefficient varied at each development phase for each variance. The actual total dry weight on the Pioneer 27 variety was 6,406 kg ha-1 with a simulated total dry weight was 6,228 kg ha-1, and the actual total dry weight on the Bisma variety was 5,127 kg ha-1 with a simulated total dry weight was 4,864 kg ha-1. The level of validity of the Pioneer 27 variety growth model reached 83.5% and the Bisma variety reached 80%. Keywords: Bisma; dry weight; growth model; nitrogen uptake; Pioneer 27; simulation; validation

Integrated Crop–Livestock Systems as a Strategy for the Sustainable Production of Corn and Soybean Grain in Tropical Sandy Soils

Integrated crop–livestock systems (ICLS) have sustainably intensified modern agricultural practices worldwide. This research assessed how production systems and crop types impact the chemical properties of an Oxisol in the Brazilian Cerrado, the grain yield of corn intercropped with palisade grass (Urochloa) in the off-season in an ICLS, and the grain yield (GY) of soybean in succession. Intercropped and monocropped systems were assessed in a three-year field experiment: corn + Urochloa ruziziensis–soybean; corn + U. brizantha cv. Piatã–soybean; corn + U. brizantha cv. Paiaguás–soybean (ICL–Paiaguás); corn–soybean under a no-tillage system (NTS); corn–soybean under a conventional tillage system (CTS); Piatã grass–continuous grazing (Perennial Piatã); and Paiaguás grass–continuous grazing (Perennial Paiaguás). The residual impact of phosphate fertilization was more pronounced in the ICLS treatments. In the soil layer from 0.0 to 0.2 m depth, ICLS–Paiaguás and Perennial Piatã had the most positive effects on soil chemical quality. In the last year, grain yield was highest in corn monoculture under the NTS and soybean in succession under the ICLS. ICL–Paiaguás improved soil chemical properties for soybean in succession. These results confirm that an intermittent pasture system for legume crops in sequence is an alternative that can maintain or improve soil chemical composition, and that CTS should be avoided in tropical sandy soils.

Forecasting Cost Risks of Corn and Soybean Crops through Monte Carlo Simulation

Considering that investing in the production of corn and soybeans is conditioned by production costs and several risks, the objective of this research work was to develop a simulation model for the prediction of the production costs of these commodities, considering the variability and correlation of key variables. The descriptive analysis of the data focused on measures such as mean, standard deviation, and coefficient of variation. To evaluate the relationship between commodity and input prices, Spearman’s demonstration coefficient and the coefficient of determination (R2) were used. A Monte Carlo simulation (MCS) was used to evaluate the variation in production costs and net revenues. The Predictor tool was used to make predictions based on historical data and time series models. This study was made for the period between 2018 and 2022 based on data provided by fifty companies from the state of São Paulo, Brazil. The results showed that the production cost/ha of corn faces a high-cost risk, particularly when production and market conditions are characterized by high levels of volatility, uncertainty, complexity, and ambiguity. The model proposed forecasts prices more accurately, as it considers the variation in the costs of inputs that most significantly influence the costs of corn and soybean crops.

Impact of Harvest Age on Corn (Zea Mays) Fodder Productivity

Corn fodder is simply seeding corn kernels that are sown until the age of 8-13 days. The harvest age of corn fodder is one way to regulate plant productivity factors. The regulation of the harvest age will affect regrowth, so it is very important to pay attention so that corn fodder plants can provide optimal production in both quantity and quality. The purpose of this study was to determine the effect of differences in harvest age on the productivity of corn (Zea mays) forage. This study used the Completely Randomized Design (CRD) method consisting of 4 treatments and 5 replications, namely T1 (harvest age 7 days), T2 (harvest age 14 days), T3 (harvest age 21 days) and T4 (harvest age 28 days). The parameters observed were plant height, number of leaves, wet biomass and dry biomass. The results showed that the average height of corn fodder plants (Zea mays) was T1; 1.11, T2; 4.96, T3; 8.30 and T4; 10. The average number of leaves of corn fodder plants (Zea mays) at T1; 0.34, T2; 1.30, T3; 1.30 and T4; 2.3. The average wet biomass of corn fodder plants (Zea mays) at T1; 197.67, T2; 249.33, T3; 246 and T4; 241.67. The average dry biomass of corn fodder plants (Zea mays) at T1; 35, T2; 47, T3; 45 and T4; 41. The conclusion of this study is that corn fodder with different harvest age variations has a very significant effect on plant height, number of leaves, wet biomass and dry biomass.

Growth and water-needs analysis of sweet corn and peanuts in different cropping systems

Maize and peanuts are important food crops in Indonesia and are generally planted in monoculture and intercropping systems. The water required for monoculture and intercropping is believed to be different. However, comparison analysis of water requirements in monoculture and intercropping is rarely conducted. The research aimed to evaluate the growth and production of sweet corn and peanuts in monoculture and intercropping systems concerning water requirement and crop coefficient. The study was conducted at IPB Experimental Station in Leuwikopo, Bogor, Indonesia from September to December 2022. Sweet corn and peanuts were planted in different cropping systems, i.e., monoculture and intercropping. Water consumption of both cropping systems was evaluated in every growing stage. The experiment used a completely randomized block design and was repeated three times. Results showed that sweet corn growth was not significantly different among cropping systems, but yield from intercropping was lower than monoculture. Intercropped peanuts grew lower than monoculture but did not show any significant difference in yield among cropping systems. Water use efficiency in intercropping was higher than monoculture for both crops, indicating intercropping is a suitable cropping system for limiting soil water availability. The land equivalency ratio was 1.80, concluding that intercropping was more efficient than monoculture in land use by 80%. Keywords: climate change; crop coefficient; intercropping; land equivalent ratio; water use efficiency

A study on the performance of corn grain dryer using hybrid solar drying with liquefied petroleum gas

Corn is a widely produced and consumed agricultural product, used as fuel, staple food, and livestock feed. The high level of corn production can lead to potential post-harvest food losses. Drying methods offer a solution to extend the shelf life of a substance. Hybrid solar drying, which combines solar drying with additional heating, particularly using liquefied petroleum gas (LPG), was introduced in this research to ensure faster and more efficient drying processes. The three drying methods compared in this study consist of open sun drying, natural solar drying, and hybrid solar drying. The research results show an average corn grain moisture content of 13.95% over 10.5 hrs, 13.75% over 6 hrs, 13.65% over 7 hrs, 13.45% over 5 hrs, and 13.65% over 4.5 hrs in open sun drying, natural solar drying, and hybrid solar drying with temperature variables of 40°C, 50°C, and 60°C. The average drying rates were 0.212 g/min, 0.364 g/min, 0.318 g/min, 0.439 g/ min, and 0.477 g/min, respectively. The average drying efficiency values for each variable were 2.968%, 4.719%, 5.358%, 5.271%, and 6.037%. In the proximate test, the corn grain moisture content in the hybrid solar drying variable at 60°C was 13.8%, ash was 1.24%, protein was 7.76%, fat was 4.40%, and carbohydrate was 72.8%. For the corn grain color test, the average values obtained were L = 46.48, a = 4.58, and b = 25.37. Meanwhile, the aflatoxin test for dried corn grains showed values of aflatoxin B1, B2, G1, and G2 <0.5 with a total aflatoxin content <2.00. Therefore, hybrid solar drying is recommended over open sun drying and natural solar drying because of its ability to operate even in unfavorable weather conditions, its temperature control capability, its ability to accelerate the drying process, and its capacity to maintain the quality of the material.

Влияние стимуляторов роста на продуктивность зерновой кукурузы

One of the most effective ways to increase grain yield per hectare is the introduction of corn for grain into the grain crop rotation, since corn is a high-yielding crop and a good precursor for spring breads, as well as a soil fertility restorer and a drought-resistant plant. Therefore, the introduction of new cultivation technologies and modern hybrids of grain corn is of great relevance at the present time. The purpose of our research was to optimize the use of growth stimulants in the cultivation of various hybrids of grain corn in the northern zone of the Krasnodar Territory. Field experiments were conducted in the production conditions of Zarya LLC in the Krylovsky district of the Krasnodar Territory in 2023 and 2024. In field experiments, three corn hybrids were used as research objects: KSS 5291, P 8834 and GS 370. In the field experiment, the scheme included options for studying the effect of pre-sowing treatment of seeds and leafy top dressing with growth stimulants on the growth, development and yield of corn. The largest root mass of grain corn was formed in the GS-370 hybrid on plots with a growth stimulant Energy M and amounted to 3,874 t/ha, which turned out to be 692 kg/ha, or 22% more than the minimum value. The maximum dry biomass in the experiment was observed in the GS-370 hybrid on plots using the growth stimulator Energy M and was 10.948 t/ha, that is, by 2.291 t/ha or 26% more than the minimum value. The maximum net photosynthesis productivity (BPF) of corn was formed in hybrid P 8834 on plots without the use of growth stimulants and was equal to 4,616 g/m2 day, that is, by 413 g/m2 day or 9.8% more than the minimum value. The highest economic yield was formed in the GS-370 hybrid, ranging from 7.08 t/ha on plots without the use of growth stimulants to 7.84 t/ha on variants using pre-sowing seed treatment and two leaf fertilizing with the Energia M stimulant.

Biofermentation of aquatic plants: Potential novel feed ingredients for dairy cattle production

The study assessed the impacts of aquatic plant silages on feeding efficiency and dairy cattle health as an alternative to conventional corn silage under high altitude conditions. Mid-lactation Holstein cows were assigned to treatment groups according to a randomized complete block design of parity, previous 105-d milk yield, and body weight. Cows (n = 8 per group) were fed with aquatic plant silage inoculated with Bacillus subtilis (BS), Yeast (YS), or conventional corn silage without inoculants (control) in addition to [standard grain feed] for 75 consecutive days. BS and YS had higher protein contents than control silage (111.20 ± 7.68, 112.10 ± 6.83 vs 76.94 ± 3.48 g/kg DM), while feeding efficiency was comparable between treatments (1.07, 0.99, and 0.90, respectively). In addition, the addition of aquatic plant silage in ruminant diets enhanced immunity and antioxidant capacity when compared with control group. Metagenomic analysis showed similar composition in rumen microbiota between YS and control groups, with higher enrichment for energy and nitrogen utilization pathways in YS-treated cows. This study highlights the use of aquatic plant silage as an alternative feed for dairy cattle with higher protein than corn silage. Our results suggest YS or BS could potentially boost immune and antioxidant functions, improving adaptation to high-altitudes and reducing demand for high input corn production on the Qinghai-Tibetan Plateau.

Response of N uptake, N-total and yield of sweet corn (Zea mays saccharata Sturt) due to fertilization with granulated solid organic fertilizer and NPK in Inceptisol soil

Sweet corn productivity is greatly influenced by proper cultivation techniques, of which fertilization is one of the main factors. Effective fertilization not only supports plant growth but also affects nutrient uptake and yield. This study aimed to evaluate the effect of granulated solid organic fertilizer and NPK fertilizer on nitrogen (N) uptake, soil N-total content, and sweet corn yield in Inceptisol soil. The research was conducted from February to June 2024 at the Experimental Field, Faculty of Agriculture, Padjadjaran University with treatments including control, NPK fertilizer, and various combinations of granular organic fertilizer and NPK. The results showed that the combination of granule organic fertilizer and NPK significantly increased nitrogen uptake and N-total content in the soil compared to the single NPK application. In addition, sweet corn yield, as measured by cob weight per plant, per plot, and per hectare, also increased significantly with the fertilizer combination treatment. This study supports the use of fertilization strategies that integrate organic and inorganic fertilizers as an effective way to increase sweet corn productivity on soils with limited fertility, such as Inceptisol. The application of a combination of 1 NPK + 1 granulated organic fertilizer was the best combination in increasing N uptake, N-total and sweet corn yield.

Furrow irrigation spacing effects on corn production in vertisols of the Mississippi Delta

Furrow irrigation spacing effects on corn production in vertisols of the Mississippi Delta

Enhancing Corn (Zea mays L.) Productivity under Varying Water Regimes with At-Plant Application of Xyway Fungicide.

A fungicide's ingredients can play a physiological role in crop water-management decisions. Our greenhouse study in 2021 demonstrated that Xyway LFR@FMC at-plant fungicide can significantly improve water-saving potential in corn. In 2022 and 2023, a field study was conducted to validate this finding. The 1.11 L ha-1 of Xyway LFR@FMC and no-fungicide/check were the main plot effects. Three water regimes, high (HI) and low (LO) numbers of irrigation events and rainfed (RF), were the subplot effect. Plants treated with Xyway LFR@FMC had significantly higher plant height, stem diameter, and leaf water potential (LWP), and had 11.9, 13.4, and 18.3% higher yield under RF, LO, and HI, respectively, in 2022. In 2023, there were no significant differences for the yield components and growth parameters when the combined effect of fungicide treatments and water regimes was considered. However, plants treated with the fungicide had a higher number of rows per ear, kernel number per row, and cob diameter compared to the check. There was no significant separation for yield among the water regimes in 2023, but the crop yield was overall higher for the fungicide-treated plots. Our results indicate that Xyway LFR@FMC fungicide has the potential to improve plant growth and protect the yield when limited water is applied.

Environmental life cycle assessment of corn production in tropical regions

Corn growing is an important cultural and traditional system in the Iran that has considerable environmental impacts. This study was conducted to determine the environmental effects of corn production using life cycle assessment methodology. In the present study, the data have been collected from face-to-face interviews and completing a questionnaire among corn farmers in Ilam province of Iran, in 2021. The functional unit for analysis was defined as one ton of corn produced in the region. As a result, energy efficiency, energy productivity, net energy and specific energy were equal to 4.41, 0.20 kg MJ−1, 675,379.84 MJ ha−1 and 4.88 MJ kg−1 respectively. Direct, indirect, renewable and non-renewable energies was 61.59, 38.41, 16.03 and 83.97%, respectively. In this study, the amount of global warming potential per ton of product produced is estimated to be 4747,505 kg CO2 equivalent. After normalization and weighting, the highest environmental pollutants are related to the marine aquatic ecotoxicity index with 225,273 kg 1,4-dichlorobenzene equivalent and the lowest pollutants are related to the depletion of ozone layer 1,19 × 10–5 kg CFC-11 equivalent. Result showed that chemical fertilizers and electricity led to the highest contaminating emissions.

Contrasting corn acreage trends in the Midwest and Southeast: The role of yield, climate, economics, and irrigation

The US corn area footprint has changed significantly since the 20th century, declining in the southeastern states while exhibiting an increase or stable variations in the Midwest. As harvested acreage directly impacts the total corn production, understanding the influencing factors is crucial. This study assesses the role of potential drivers on the contrasting trajectories of harvested corn acreage between midwestern and southeastern US. Profit-acreage analysis reveals that antecedent profits/losses have a statistically significant influence on corn acreage changes, with southeastern US, which experienced more loss-making years, also experiencing more frequent reductions in corn acreage. The high number of loss-making years in the Southeast is primarily attributed to the region's low corn yield, influenced by climate and other agro-environmental factors. Using a panel regression model, we find that the loss-making years in the Southeast could have reduced to fewer than 26 out of the considered 45 years, or almost similar to the average in the Midwest, by just increasing the irrigated corn area to 50 %, a realistic irrigated corn area fraction already achieved in several Georgia counties. This underscores the potential for early policy interventions like irrigation facilitation to sustain and expand cropped acreage. However, we also find that this would only be economically feasible with incentives for both the installation and sustained operation of irrigation infrastructure.

Dissipation pattern and dietary risk assessment of chlorfenapyr and methomyl in corn under Egyptian field conditions.

Corn is the second most widely farmed grain for human consumption. Low corn productivity due to damage caused by pests has led to using pesticides to control pest infestations. However, the uncontrolled application of pesticides on corn harms both environmental and human health. Accordingly, field experiments followed good agricultural practices to investigate the dissipation pattern and terminal residues of chlorfenapyr and methomyl in corn and compare the values with established safety limits. Gas chromatography-tandem mass spectrometer coupled with the quick, easy, cheap, effective, rugged, and safe technique was used to analyze residues of chlorfenapyr and methomyl in corn. The average recoveries varied from 94% to 105%, with relative standard deviations (RSDs) of 8%-13% for chlorfenapyr and from 99% to 111%, with RSDs of 10-16% for methomyl. Chlorfenapyr and methomyl residues degraded in corn following a first-order kinetic model, with an estimated half-life (t1/2) of 3.9 and 2.8days, respectively, and significant degradation (91.4%-98.1.5%, respectively) after 14 days. Although the maximum residue limits of chlorfenapyr and methomyl for corn are yet to be formulated in Egypt, the long-term dietary risk for those pesticides was acceptable, with arisk quotient < 100%, according to the national assessments. These findings are required to guide the correct and safe application of these insecticides in Egypt.

Production Risk of Hybrid Maize in Sugihmanik Village, Tanggungharjo Subdistrict, Grobogan Regency (Case Study: Faisal Tani Store)

This study aims to examine the impact of production factors on hybrid corn production and to assess the level of production risk in Sugihmanik Village, Tanggungharjo District, Grobogan Regency (Case Study: Faisal Tani Store). The research adopts a quantitative approach with a sample of 69 hybrid corn farmers. The data analysis method utilized the Cobb-Douglas production function with multiple linear regression and coefficient of variation analysis. Data processing was conducted using SPSS 25. The results indicated that capital, land area, and labor factors collectively influence hybrid corn production. In addition, the land area and labor variables were found to have a significant effect on hybrid corn production, while the capital variable did not show a significant effect. The coefficient of variation (CV) for production risk in hybrid corn farming in Sugihmanik Village, Tanggungharjo District, Grobogan Regency (Case Study: Faisal Tani Store) was 0.33, indicating a low level of production risk.

Implementation of Management Function to Corn fritter Enterpreneurs in Padang Panjang City

This research is based on the background of corn business actors who are still facing challenges in applying management principles so that the production produced is not effective and efficient. The purpose of the study is to describe production management in corn farming business actors in Padang Panjang City. The type of research used is qualitative, carried out in Padang Panjang City in July 2024. The source of research data was obtained from informants, namely corn business actors. Data collection techniques by conducting observations, interviews, and documentation. The data analysis used includes data reduction, data presentation, and conclusion drawn. The results of the study show that corn farmers in Padang Panjang City are quite good in carrying out management functions, namely: planning, organizing, directing and supervising the corn production process.

The Effect of NASA Liquid Organic Fertilizer and Chicken Manure on the Growth of Sweet Corn Plants (Zea mays saccharata Sturt)

This research aims to determine the effect of a combination of NASA POC and chicken manure on the growth and production of sweet corn (Zea mays saccharata Sturt). The research used a Randomized Group Design (RAK) with two treatment factors. The first factor is the administration of NASA Liquid Organic Fertilizer (A) consisting of 4 treatment levels, namely: A0 = 0 ml/liter of water (control), A1 = 2 ml/liter of water, A2 = 4 ml/liter of water and A3 = 6 ml /liter of water. The second factor is the provision of chicken manure (B) consisting of 3 treatment levels, namely: B1 = 2.25 kg/plot (7.5 tons/ha), B2 = 4.50 kg/plot (15 tons/ha) and B3 = 6.75 kg/plot (22.5 tons/ha). The results showed that giving NASA POC up to 6 ml/l of water significantly increased plant height, ear length, ear weight per plot and total dissolved solids. Providing chicken manure up to 6.75 kg/plot significantly increased plant height, ear length, weight per plot and total soluble solids. The interaction between NASA POC and chicken manure did not significantly affect plant height, ear length, weight per plot, or total dissolved solids.

Occurrence of Maize Leaf Spot Disease Caused by Leptosphaerulina australis in Yunnan, China.

Maize (Zea mays) is vital as a staple food and livestock feed crop. Yunnan is one of the main maize-producing provinces in China (National Bureau of Statistics, 2022). While corn production in Yunnan is lower than the national average, the development of drought-tolerant varieties has contributed to improving productivity. In August 2021, a new leaf spot disease on maize was observed in Lancang, Yunnan (22°26'38.11"N to 22°48'38.68"N, 99°48'15.13"E to 99°59'20.03"E), causing serious damages to maize production with incidence up to 76.19 %. Initially, small light yellow lesions were seen scattered on diseased maize leaves, round or polygon, measuring 0.3 to 2.0 cm in diameter. In the intermediate phase, these lesions sank, ruptured, and turned white with dark brown borders. In severe cases, they merged into large irregular patches, reaching up to 10 cm, leading to complete leaf necrosis. Small black ascomata were seen on the lesions. Tissue sections reveal perithecium embedded in leaves, measuring 94~145 μm in diameter. Symptomatic tissues were sterilized in 1.5% NaClO for 60s, and washed twice withsterile purified water, then plated on potato dextrose agar (PDA) at 25℃, 90% relative humidity (RH), and a 12-hour light cycle. 6 isolates were obtained from 2 diseased maize cultivars. In 20 days, the colony reached the edge of the PDA plate, the center darkening from white, featuring white aerial mycelium on top and black on the reverse side. Brown ascomata, solitary or clustered, measured 80.1~176.7 × 55.57~138.9 μm. The ellipsoid to oblong ascospores were 17.9~39.7 × 10.9~14.1 μm, and the bitunicate, thick-walled asci were 90.1~133.3 × 26.6~33.5 μm. The genomic DNA was extracted using the Chelex-100 method (Möller et al. 1992). For molecular identification, the ITS, LSU, and β-tubulin (Tub2) genes were amplified using primer pairs ITS1/ITS4 (White et al. 1990), LR0R/LR5 (Vilgalys et al. 1990) and Btub2Fd/Btub4Rd (Woudenberg et al. 2009), respectively. Sequencing was performed by Sangon Biotech (Shanghai) Co., Ltd. The sequenced loci (GenBank accession nos.: LSU, OL687348-53; ITS, OL617009-10, and OL664058-61; Tub2, OL741678-83) of the isolates exhibited 100%/ 99%/ 100% similarities with L. australis genes: LSU, MH868885; ITS, KF381084; Tub2, GU237541, respectively. Using MEGA 11.0, phylogenetic trees were constructed using the maximum-likelihood algorithm on concatenated sequences of LSU, ITS, and Tub2 for isolates LCMB1 to 6. The isolates clustered with two L. australis strains with 100 % bootstrap support (1,000 replicates). The results were consistent with the Bayesian Inference tree. The pathogenicity test used strain LCMB4 on six healthy maize plants during the heading period under natural conditions. Three leaves pre-plant were wounded with sterile sandpaper and sprayed with conidial suspension (106 spores ml-1, diluted in sterilized water) in the greenhouse at 28℃, 90% RH, and a 12-hour light cycle, with sterilized distilled water used for control. Inoculated leaves developed symptoms consistent with the described after 10 days, while control leaves remained symptomless. The same pathogen was re-isolated from the infected leaves, fulfilling Koch's postulates. Previously, L. australis has been isolated from turfgrass (Mitkowski et al. 2004), Alfalfa (Zhang et al. 2021), soil (Li et al. 2018), and Paris polyphylla var. chinensis (Fu et al. 2019), but not from maize. This is the first report of L. australis causing leaf spot on maize globally.