Development Of Production Systems Research Articles

Cell-Specific Impacts of Surface Coating Composition on Extracellular Vesicle Secretion.

Biomaterial properties have recently been shown to modulate extracellular vesicle (EV) secretion and cargo; however, the effects of substrate composition on EV production remain underexplored. This study investigates the impacts of surface coatings composed of collagen I (COLI), fibronectin (FN), and poly l-lysine (PLL) on EV secretion for applications in therapeutic EV production and to further understanding of how changes in the extracellular matrix microenvironment affect EVs. EV secretion from primary bone marrow-derived mesenchymal stromal cells (BMSCs), primary adipose-derived stem cells (ASCs), HEK293 cells, NIH3T3 cells, and RAW264.7 cells was characterized on the different coatings. Expression of EV biogenesis genes and cellular adhesion genes was also analyzed. COLI coatings significantly decreased EV secretion in RAW264.7 cells, with associated decreases in cell viability and changes in EV biogenesis-related and cell adhesion genes at day 4. FN coatings increased EV secretion in NIH3T3 cells, while PLL coatings increased EV secretion in ASCs. Surface coatings had significant effects on the capacity of EVs derived from RAW264.7 and NIH3T3 cells to impact in vitro macrophage proliferation. Overall, surface coatings had different cell-specific effects on EV secretion and in vitro functional capacity, thus highlighting the potential of substrate coatings to further the development of clinical EV production systems.

Enhancing extracellular membrane vesicle productivity of Shewanella vesiculosa HM13, a prospective host for vesiculation-mediated protein secretion, by weakening outer membrane-peptidoglycan linkage

Shewanella vesiculosa HM13, a psychrotrophic gram-negative bacterium isolated from the intestinal contents of horse mackerel, produces abundant extracellular membrane vesicles (EMVs) by budding the outer membrane. The EMVs of this bacterium carry a single major cargo protein, P49, of unknown function, which may be useful as a carrier for the secretory production of heterologous proteins as cargoes of EMVs. In this study, to increase the utility of S.vesiculosa HM13 as a host for EMV-mediated protein production, we improved its EMV productivity by weakening the linkage between the outer membrane and underlying peptidoglycan layer. In gram-negative bacteria, the outer membrane is connected to peptidoglycans predominantly through Braun's lipoprotein (Lpp), and the formation of this linkage is catalyzed by an l,d-transpeptidase (Ldt). We constructed gene-disrupted mutants of Lpp and Ldt and assessed their EMV productivity. The EMVs of the lpp- and ldt-disrupted mutants grown at 18°C were evaluated using nanoparticle tracking analysis, and their morphologies were observed using transmission electron microscopy. As a result, an approximately 2.5-fold increase in EMV production was achieved, whereas the morphology of the EMVs of these mutants remained almost identical to that of the parent strain. In accordance with the increase in EMV production, the mutants secreted approximately 2-fold higher amounts of P49 than the parent strain into the culture broth as the EMV cargo. These findings will contribute to the development of an EMV-based secretory production system for heterologous proteins using S.vesiculosa HM13 as a host.

Urban landscape sustainability in karst mountainous cities: A landscape resilience perspective

In the context of the rapid progress of global urbanization, the massive encroachment of social landscapes into ecological and productive landscapes has led to a series of environmental problems. Furthermore, analyzing the landscape resilience could effectively reveal the sustainable development ability of the urban landscape. This study establishes a social-ecological productive landscape resilience (SEPLR) evaluation system and reveals trade-offs and synergies between different landscape types and resilience. Finally, this study provides landscape management zonings based on the spatial and temporal dynamic characteristics of landscape resilience and subsystem resilience. The findings showed that: (1) The CUAG has significant landscape heterogeneity and change drastically, which is mainly manifested by the massive encroachment of social landscape into productive landscape. (2) The SEPLR of CUAG decreased slightly by 0.75 % over the decade, with significant changes of spatial distribution. (3) The comprehensive remediation areas and social development areas are the dominant management zones. The findings could be incorporated into the decision-making of land use trade-off development in CUAG to promote the coordinated development of social-ecological productive systems and improve the sustainability of urban landscape.

Роль технологического предпринимательства в сфере промышленности в современных условиях

Introduction. The competitiveness of industry in modern conditions is carried out through scientific and technological development. High-tech companies play a key role in solving the main tasks of increasing the competitiveness of Russian economy in accordance with global challenges and priorities of Russia’s scientific and technological development. Theoretical analysis. High-tech production, Industry 4.0 technologies and technological entrepreneurship in modern economy are the main actors in achieving technological sovereignty, the transition to economic growth and technological support for the sustainable development of Russian production systems. The article examines the role of technological entrepreneurship in the transition of industrial enterprises to Industry 4.0 technologies. Empirical analysis. An innovative development dynamics analysis in Russia and foreign countries is carried out, a startup studio is considered as a tool for the development of technological entrepreneurship, as well as the practice of its implementation in Russia and the USA. Results. Based on the results of the study, a number of proposals for the development of technological entrepreneurship in the small and medium-sized enterprise industry sector were formulated and justified.

Digital twins in aircraft production and MRO: challenges and opportunities

The digital twin (DT) concept, value-adding connecting the real and digital world, has been a rising trend in recent years, while the implementation and observation of challenges are still subject to research. Implementations of holistic Digital Twins of tangible and intangible assets of complex products or processes are often ideal-theoretic; instead, only subsystems and processes are replicated, which digital representations serve specific, meaningful applications. Specifically, with its distinct characteristics, the aviation industry and its production show various future application scenarios, which we use case-driven outline in this work. Therefore, we first summarize common, industry-neutral challenges of implementing Digital Twins and give an overview of aircraft production characteristics. Then, we will outline different fields of utilizing the Digital Twin concept and highlight integrational, organizational, and compliance-related challenges as well as opportunities in the context of aircraft production and Maintenance, Repair, and Overhaul (MRO). The use cases are located at different aircraft life cycle phases, from production system development, production supplying logistics, and Quality Assurance (QA) up to retrofit.

Challenges of the integrative product and production system development

AbstractShorter product lifecycles and a shift from mechatronic to cyber-physical systems are leading to greater product complexity. This complexity can be addressed by more intensive cooperation between product and production system development. Despite intensive development in recent years, these process models have not been able to establish themselves in corporate practice. Therefore, this paper analyses the existing integrative product and production methods to identify the reasons for their lack of use in practice. The analysis has shown that there are nine barriers.

Towards the digital factory twin – design guide for creating a 3D factory model

AbstractShorter product lifecycles are also leading to even shorter planning times for the development of production systems. In most companies, the restructuring is carried out within a few weeks during the annual holidays. Digital tools such as simulations or the digital twin are used to avoid delaying the restructuring during this time. However, the introduction of a 3D model of the factory is often the first point of failure for many companies. This article proposes a six-step process model that enables the transition from 2D to 3D design. The process model was evaluated in a research project.

EVALUATING THE IMPACT OF CROSS-CORRELATION PROPERTIES OF COMPLEX SIGNALS ON THE CHARACTERISTICS OF SMART RADIO SYSTEMS

The article discusses modern problems and prospects for the development of "smart radio" systems using complex signals for effective use of the radio frequency spectrum. Cognitive radio systems use complex signals to efficiently use the radio frequency spectrum. It is emphasized that the ensemble size of complex signals is a key factor that affects various system characteristics such as immunity, data transmission efficiency, and spectral efficiency. Various methods can be used to increase the ensemble size of complex signals, including the use of pseudo-random sequences with low levels of sidelobes in the cross-correlation function, new complex signal generation algorithms (e.g., based on artificial intelligence), and new coding techniques that allow for increased ensemble size. without compromising data transmission efficiency. The choice of a specific approach to increasing the size of the ensemble of complex signals depends on the specific requirements of the cognitive telecommunication system. An important task is the development of new methods of generating complex signals with high interference-resistant properties, which allow to increase the size of the ensemble without compromising the quality of service. This task is important for the development of next-generation "smart radio" systems. The analysis of various methods of increasing the size of the ensemble of complex signals is presented. The maximum correlation immunity is calculated for different lengths of sequences and the results of research for five sequences are given: pseudorandom noise, nonlinear sequence, Galois sequence, Gold sequence and Kiyosaki sequence. This information can be used to select the optimal sequence taking into account the specific requirements of "smart radio" systems. The conclusions of the article can make a valuable contribution to the development of effective and productive telecommunication systems for future generations of "smart radio".

High-performance heterometallic photocatalysts afforded by polyoxometalate synthons for efficient H2 production

MoS2-based materials have emerged as photoelectric semiconductors characterized by a narrow band gap, high capacity for absorbing visible light, and reduced H2 adsorption energy comparable to Pt. These attributes render them appealing for application in photocatalytic hydrogen production. Despite these advantages, the widespread adoption of MoS2-based materials remains hindered by challenges associated with limited exposure to active sites and suboptimal catalytic hydrogen production efficiency. To address these issues, we have designed and synthesized a new class of highly dispersed bimetallic/trimetallic sulfide materials. This was achieved by developing polyoxometalate synthons containing Ni-Mo elements, which were subsequently reacted with thiourea and CdS. The resulting Ni3S2-MoS2 and Ni3S2-MoS2-CdS materials achieve photocatalytic hydrogen production rates of 2770 and 2873 μmol g–1h−1, respectively. Notably, the rate of 2873 μmol g–1h−1 for Ni3S2-MoS2-CdS surpassed triple (3.23 times) the performance of CdS and nearly sextuple (5.77 times) that of single MoS2. These materials outperformed the majority of MoS2-based photocatalysts. Overall, this study introduces a straightforward methodology for synthesizing bimetallic/trimetallic sulfides with enhanced photocatalytic H2 evolution performance. Our findings underscore the potential of transition metal sulfide semiconductors in the realm of photocatalysis and pave the way for the development of more sustainable energy production systems.

Pendampingan dan Pengembangan Sistem Pengolahan Sampah serta Perancangan Produk Pupuk Cair Bernilai Market sebagai Produk Inovasi di Kelurahan Perwira Kota Bekasi Jawa Barat

Waste management using the Reuse, Reduce and Recycle method or known as 3R has been introduced to the public by the government, researchers and other institutions more than 10 years ago. This method is effective in reducing the volume of waste and also the negative impacts caused by waste. In the Perwira sub-district, Bekasi City has implemented waste management with this method but is limited to inorganic waste. This paper reports on the program for developing an organic waste management system into a community-based innovation product. This activity consists of a quantitative study of waste production, workshops on waste management to become innovative products and have market value. The results of the evaluation of this activity show that there is an increase in public awareness of organic waste management, increased insight into innovative product development systems and digital marketing strategies. More than 70% of the community felt that this activity was useful and needed to be continued.

THE POTENTIAL OF VIRTUAL REALITY AND ARTIFICIAL INTELLIGENCE IN MODELING PRODUCT INNOVATIONS OF VIRTUAL CORPORATION

This scientific article explores the characteristics of modeling product innovations in virtual corporations that operate in online environments (without physical offices). The authors note that the proliferation of virtual reality and artificial intelligence in modeling product innovations for virtual corporations holds great potential. According to the provisions outlined, the article aims to determine the potential of virtual reality and artificial intelligence in modeling product innovations for virtual corporations. The research demonstrates that while the key characteristics of effective product innovations for virtual corporations are shaped by advancements in information processing, organizational dynamics, and the development of production systems, virtual reality, and artificial intelligence tools create the potential for their modeling. The practical significance of the research lies in expanding the knowledge about the impact of VR and AI tools on the processes of modeling product innovations and in shaping opportunities for developing strategies for their effective utilization.

Water-land-Food Production System Interconnections

Water-land-food (WLF) is a fundamental resource that supports human survival and development. In the 21st century, with the acceleration of industrialization and urbanization in developing countries, the phenomena of industrial water use squeezing agricultural water use and land encroachment by construction land are very common. To a certain extent, this has aggravated the problems of irrational allocation of water resources in the production system, shortage of land resources and food security. Against this background, this study, based on a large number of literature searches, firstly, summarizes the research progress of water-land-food production system. Secondly, from the perspective of "interconnectedness" of WLF, we summarize the basic resource relationships among the three production systems and construct a systematic study of WLF production systems. Finally, based on the comparison with related studies, it is proposed that the future research focus of WLF production system includes: (1) Regional WLF production system research. (2) Resource regulation of regional WLF production system. (3) benign development of WLF production system under ecological environment. (4) Sustainability of WLF production system under food security.

Higher Plant-Derived Biostimulants: Mechanisms of Action and Their Role in Mitigating Plant Abiotic Stress.

Modern agriculture is being challenged by deteriorating edaphoclimatic conditions and increasing anthropogenic pressure. This necessitates the development of innovative crop production systems that can sustainably meet the demands of a growing world population while minimizing the environmental impact. The use of plant biostimulants is gaining ground as a safe and ecologically sound approach to improving crop yields. In this review, biostimulants obtained from different higher plant sources are presented under the term higher plant-derived biostimulants (hPDBs). Their mechanisms of action regulate physiological processes in plants from germination to fructification, conditioned by responses induced in plant mineral nutrition and primary metabolism, specialized metabolism, photosynthetic processes, oxidative metabolism, and signaling-related processes. The aim of this review is to collect and unify the abundant information dispersed in the literature on the effects of these biostimulants, focusing on crops subjected to abiotic stress conditions and the underlying mechanisms of action.

Water Footprints of Dairy Milk Processing Industry: A Case Study of Punjab (India)

A robust assessment of water used in agriculture, including livestock production systems and supply chains, is critical to inform diversification and the development of productivity and sustainable food production systems. This paper presents a detailed analysis of water used and consumed in nine dairy milk processing plants spread across Punjab, India’s leading dairy milk-producing state. Over the five years (2015–2019), the direct water use (DWU) was quantified at 3.31 L of groundwater per kg of milk processed. Only about 26% of the direct water used was consumed, including evaporative losses in various milk processing operations, while the remaining 74% was returned as effluent discharges. The average total water footprint (TWF), accounting for both direct and indirect water consumption, was quantified at 9.0 L of water per kg of milk processed. The majority share (~89%) of the total water footprint was contributed by the indirect water footprint associated with the consumption of electricity (energy) in dairy milk processing activities. The plant’s milk processing capacity and processing products mix also affected significant seasonal and annual variations in the direct and indirect water footprints of dairy milk processing. The analysis also found an inverse relationship between the average total water footprint and the average monthly amount of milk processed in the study plants. Therefore, efforts to reduce the indirect water footprint (associated with energy consumption), the treatment and recycling of effluent discharges, and the optimization of milk processing capacity, the dairy processing product mix, and the locations of dairy processing plants are expected to help reduce the water footprint of dairy processing in the state.

Spatiotemporal Evolution Characteristics and Driving Factors of Water-Energy-Food-Carbon System Vulnerability: A Case Study of the Yellow River Basin, China

With the growing influences of anthropogenic activities and climatic change, the problem concerning the vulnerability of the Water-Energy-Food-Carbon (WEFC) system in the Yellow River Basin is prominent. Using the VSD framework, the WEFC system vulnerability evaluation index system was constructed with 60 cities in the Yellow River Basin as the samples, and the WEFC system vulnerability of each city was measured from 2010 to 2019. Kernel density estimation, Theil index, and spatial correlation analysis were employed to investigate spatio-temporal evolution characteristics. Geodetector was utilized to analyze the driving factors of WEFC system vulnerability. The results demonstrate that: (1) The vulnerability of the WEFC system in the Yellow River Basin tends to decrease, with a spatial pattern of “low in the middle and high on both sides”; the vulnerability is largest in the upper and lower reaches, while smallest in the middle reaches. (2) The spatial difference in vulnerability narrows in the middle and lower reaches, while expanding in the upper reaches. The differences among the three major regions mainly originate from within the region, with the upper reaches having the largest difference and contribution; the vulnerability is featured with a significant spatial correlation, with the upper and lower reaches cities mostly displaying a “high-high” agglomeration and the middle reaches mainly showing a “low-low” one. (3) Factors, including the carbon and ecological carrying capacity coefficient, water resource development and utilization rate, and urbanization rate, mainly influence the WEFC system vulnerability; the spatial heterogeneity of core drivers at the regional scale is obvious, with the upper reaches regions being more strongly influenced by factors of the water resources system, while the middle and lower reaches regions are more sensitive to factors concerning industrial pollution of the energy subsystem. The explanatory power of carbon ecological carrying capacity reaches its peak in the middle reaches. The interaction of factors increases the strength of the impact on vulnerability. This study provides decision support and policy suggestions for achieving a balanced and coordinated development of water resource utilization, energy development, food production, and carbon cycle system in the Yellow River Basin. Investigating WEFC system vulnerability to support SDG 11 provided valuable insights and policy strategies for building cities that are inclusive, secure, resource-efficient, and resilient in the face of climate change and disaster risks.

Investigation of the potential of yeast strains for phytase biosynthesis in a two-step screening procedure

Research into phytase production is useful for improving the efficiency of animal production, reducing environmental impact, and contributing to the development of sustainable and efficient animal production systems. This study aims to investigate the potential of yeast strains for phytase biosynthesis in nutrient media. Phytase is a phosphomonoesterase (E.C 3.1.3.8) catalyzing in a ladder-like manner the dephosphorylation of phytic acid and its salts, with various resulting myo-inositol phosphates and phosphoric acid. Yeasts of the genera Saccharomyces, Zygosaccharomyces, Candida, and Pichia were evaluated in a two-step screening procedure for phytase production. One hundred and eighteen strains were screened in the first stage, which was conducted on four types of solid culture media containing calcium phytate as the selected background. On PSM medium, many strains were found to form halos as early as the 24th hour of development. Several strains with significant potential for enzyme production were evaluated in the second step of the screening. It was conducted in a liquid culture medium. In conclusion, the strain C. melibiosica 2491 was selected for further studies when cultured in a YPglu culture medium. Further research will focus on finding suitable conditions that increase the biosynthesis of the enzyme, which is of significant technological and practical interest for animal nutrition.

Measurement of the level of high-quality agricultural development and analysis of spatial differences - An empirical study based on panel data in the Yangtze River Delta region

In the context of China entering the overlapping period of common prosperity and rural revitalization, rural revitalization under the vision of common prosperity is an important practical issue, and improving the utilization rate of agricultural resources is conducive to exploring the optimal path of improving the utilization efficiency of arable land resources, realizing the modernization of agriculture and assisting in rural revitalization. Based on the panel empirical data in the Yangtze River Delta region, this study firstly summarizes the research hotspots and trends of "high-quality development of agriculture" at home and abroad. Secondly, based on the relevant theories, we construct a theoretical framework for measuring the level and spatial differentiation of "high quality of agriculture", which contains "two sides and five basic dimensions". Then, the stata stepwise regression method is used to eliminate highly correlated variables, establish a comprehensive indicator system for measuring the trend of "regional agricultural production system and yield high quality development", and utilize the entropy value method to measure the trend of regional change in the comprehensive agricultural industry. Combining the five basic agricultural dimensions, analyze what factors affect the level of high-quality development of agriculture and spatial distribution differences. Combining the results of the analysis of the factors affecting the level of high-quality development and spatial differences in agriculture, we will draw a conclusion on the sustainable development of agriculture, and provide an appropriate way of operation for the strategy of high-quality integrated development of agriculture in the Yangtze River Delta region.

Development of a tag-free plant-made interferon gamma production system with improved therapeutic efficacy against viruses.

Plants offer a promising platform for cost-effective production of biologically active therapeutic glycoproteins. In previous studies, we have developed a plant expression system based on Bamboo mosaic virus (BaMV) by incorporating secretory signals and an affinity tag, which resulted in notably enhanced yields of soluble and secreted fusion glycoproteins (FGs) in Nicotiana benthamiana. However, the presence of fusion tags on recombinant glycoproteins is undesirable for biomedical applications. This study aimed to develop a refined expression system that can efficiently produce tag-free glycoproteins in plants, with enhanced efficacy of mature interferon gamma (mIFNγ) against viruses. To accommodate the specific requirement of different target proteins, three enzymatically or chemically cleavable linkers were provided in this renovated BaMV-based expression system. We demonstrated that Tobacco etch virus (TEV) protease could process the specific cleavage site (LTEV) of the fusion protein, designated as SSExtHis(SP)10LTEV-mIFNγ, with optimal efficiency under biocompatible conditions to generate tag-free mIFNγ glycoproteins. The TEV protease and secretory-affinity tag could be effectively removed from the target mIFNγ glycoproteins through Ni2+-NTA chromatography. In addition, the result of an antiviral assay showed that the tag-free mIFNγ glycoproteins exhibited enhanced biological properties against Sindbis virus, with comparable antiviral activity of the commercialized HEK293-expressed hIFNγ. Thus, the improved BaMV-based expression system developed in this study may provide an alternative strategy for producing tag-free therapeutic glycoproteins intended for biomedical applications.

Effects of altered gravity on growth and morphology in Wolffia globosa implications for bioregenerative life support systems and space-based agriculture

Understanding the response of plants to varied gravitational conditions is vital for developing effective food production in space bioregenerative life support systems. This study examines the impact of altered gravity conditions on the growth and morphological responses of Wolffia globosa (commonly known as “water lentils” or “duckweed”), assessing its potential as a space crop. Although an experiment testing the effect of simulated microgravity on Wolffia globosa has been previously conducted, for the first time, we investigated the effect of multiple gravity levels on the growth and morphological traits of Wolffia globosa plants. The plant responses to simulated microgravity, simulated partial gravity (Moon), and hypergravity environments were evaluated using random positioning machines and the large-diameter centrifuge. As hypothesized, we observed a slight reaction to different gravitational levels in the growth and morphological traits of Wolffia globosa. The relative growth rates (RGR) of plants subjected to simulated microgravity and partial gravity were reduced when compared to those in other gravity levels. The morphological analysis revealed differences in plant dimensions and frond length-to-width ratios under diverse gravity conditions. Our findings showed that Wolffia globosa is responsive to gravitational changes, with its growth and morphological adaptations being slightly influenced by varying gravitational environments. As for other crop species, growth was reduced by the microgravity conditions; however, RGR remained substantial at 0.33 a day. In conclusion, this study underscores the potential of Wolffia globosa as a space crop and its adaptability to diverse gravitational conditions, contributing to the development of sustainable food production and bioregenerative life support systems for future space exploration missions.

Designer-centric and function-oriented development framework for complex industrial products

Derived from the RFLP method (requirements, functions, logic, product) of model-based systems engineering, a function-oriented development approach can enable a universal, data and model-driven knowledge management system for complex product development, facilitate cross-disciplinary development, and support innovation. While function-orientation has been investigated from a technological standpoint, the successful implementation of such a concept is also dependent on human factors, which have not yet been sufficiently explored. This study aims to understand the needs of the designers for the implementation of the function-oriented development framework. Using a human-centred design approach, a study was conducted where the current version of the function-oriented framework was tested with potential users from the automotive industry. The basics of the framework were implemented in a prototype to immerse the subjects in the workflow. The subjects developed a fictitious product subsystem on the prototype using the framework. The results of the study illustrate the potential benefits, challenges, and implementation ideas for the framework from the designer perspective. The valuable insights from this industrial study can be used to develop a designer-centric function-oriented development framework and derive introduction and implementation strategies for large organizations.