Preparation Of Standards Research Articles

Pathways to Identify Electrophiles In Vivo Using Hemoglobin Adducts: Hydroxypropanoic Acid Valine Adduct and Its Possible Precursors.

Analytical methods and tools for the characterization of the human exposome by untargeted mass spectrometry approaches are advancing rapidly. Adductomics methods have been developed for untargeted screening of short-lived electrophiles, in the form of adducts to proteins or DNA, in vivo. The identification of an adduct and its precursor electrophile in the blood is more complex than that of stable chemicals. The present work aims to illustrate procedures for the identification of an adduct to N-terminal valine in hemoglobin detected with adductomics, and pathways for the tracing of its precursor and possible exposure sources. Identification of the adduct proceeded via preparation and characterization of standards of adduct analytes. Possible precursor(s) and exposure sources were investigated by measurements in blood of adduct formation by precursors in vitro and adduct levels in vivo. The adduct was identified as hydroxypropanoic acid valine (HPA-Val) by verification with a synthesized reference. The HPA-Val was measured together with other adducts (from acrylamide, glycidamide, glycidol, and acrylic acid) in human blood (n = 51, schoolchildren). The HPA-Val levels ranged between 6 and 76 pmol/g hemoglobin. The analysis of reference samples from humans and rodents showed that the HPA-Val adduct was observed in all studied samples. No correlation of the HPA-Val level with the other studied adducts was observed in humans, nor was an increase in tobacco smokers observed. A small increase was observed in rodents exposed to glycidol. The formation of the HPA-Val adduct upon incubation of blood with glycidic acid (an epoxide) was shown. The relatively high adduct levels observed in vivo in relation to the measured reactivity of the epoxide, and the fact that the epoxide is not described as naturally occurring, suggest that glycidic acid is not the only precursor of the HPA-Val adduct identified in vivo. Another endogenous electrophile is suspected to contribute to the in vivo HPA-Val adduct level.

The potential of bioprinting for preparation of nanoparticle-based calibration standards for LA-ICP-ToF-MS quantitative imaging.

This paper discusses the feasibility of a novel strategy based on the combination of bioprinting nano-doping technology and laser ablation-inductively coupled plasma time-of-flight mass spectrometry analysis for the preparation and characterization of gelatin-based multi-element calibration standards suitable for quantitative imaging. To achieve this, lanthanide up-conversion nanoparticles were added to a gelatin matrix to produce the bioprinted calibration standards. The features of this bioprinting approach were compared with manual cryosectioning standard preparation, in terms of throughput, between batch repeatability and elemental signal homogeneity at 5μm spatial resolution. By using bioprinting, the between batch variability for three independent standards of the same concentration of 89Y (range 0-600mg/kg) was reduced to 5% compared to up to 27% for cryosectioning. On this basis, the relative standard deviation (RSD) obtained between three independent calibration slopes measured within 1 day also reduced from 16% (using cryosectioning) to 5% (using bioprinting), supporting the use of a single standard preparation replicate for each of the concentrations to achieve good calibration performance using bioprinting. This helped reduce the analysis time by approximately 3-fold. With cryosectioning each standard was prepared and sectioned individually, whereas using bio-printing it was possible to have up to six different standards printed simultaneously, reducing the preparation time from approximately 2 h to under 20 min (by approximately 6-fold). The bio-printed calibration standards were found stable for a period of 2 months when stored at ambient temperature and in the dark.

In Situ Validation Methodology for Weighing Methods Used in Preparing of Standardized Sources for Radionuclide Metrology

High-accuracy source standards preparation in radionuclide metrology is based on a properly described and reliable weighing procedure able to achieve relative standard uncertainties below 0.1%. However, the results of uncertainty budget comparison CCRI(II)-S7 put in check the ability of the former pycnometer and substitution weighing methods to attain this goal. As a result, a question arises about the validation of mass measurements performed from the elimination weighing method when appropriate uncertainties are required. In order to address this problem, a comprehensive in situ validation methodology is proposed for the results of the pycnometer, substitution, elimination and modified elimination (MEM) methods. Mass comparisons are applied to evaluate the compatibility between weighing methods’ results. It is possible due to a developed weighing sequence, which allows for the performing of all methods by only one drop deposition in the range of mass from 10 mg to 200 mg. As a result, the high degree of compatibility between the MEM and elimination method for uncertainties below 0.1% has been achieved, as well as for higher uncertainties to pycnometer and substitution methods. Numerical simulations indicate that the validation results remain valid on improved technical implementations for these last two methods.

Implementasi Kebijakan Ketenagakerjaan Di Kabupaten Bekasi

This thesis is entitled Implementation of Labour Policy in Bekasi Regency which raises the issue of how the implementation of labour policy in Bekasi Regency is seen from its dimensions and determinants and how to improve labour services in order to increase job opportunities in the Bekasi Regency area. The theory used as an analytical knife in writing this thesis is the theory of policy implementation model according to Muchlis Hamdi (2014) which explains the description of the movement dynamics of policy implementation, namely the dimensions of productivity, linearity and efficiency and the factors that can affect the success of policy implementation, namely the determinants of policysubstance, behavior of implementing tasks, network interactions, target group participation and resources. The method used is a qualitative approach, data collection techniques are interviews, document review, observation, and triangulation. Based on the data and analysis of the implementation of labour policies in Bekasi Regency, seen from the dimensions and determinants, some results are obtained that the implementation of labour policies in Bekasi Regency has been going quite well which can be seen from the achievement of performance targets and SPM increased from 2018 and can meet the target in 2019, the use of funds, infrastructure and implementing officers in supporting employment services is quiteefficient, the contents of employment policies, especially employment services with other policies are aligned , the level of performance and ability to make the initiative of implementing officers is quite good, the interaction of the network of implementing officers is quite harmonious, the participation of the target group both job seekers and company on the implementation of labour policies in Bekasi Regency is quite high, resource support is good, adequate funds,availability of implementers and the availability of equipment for the implementation of labor policies in Bekasi Regency is quite good. However, in order to improve employment services in the context of increasing job opportunities, apartfrom optimizing things that are already going well, it is necessary to make efforts, including the preparation ofstandard operating procedures (SOPs) in overall employment services, drafting Regent regulations to regulate technical implementation guidelines. Bekasi Regency regional regulation number 4 of 2016 concerning Employment, increasing competence for functional officers who are equal to work delivery results, increasing network interaction with employees of the labour inspector Office of West Java Province, increasing the competence and skills of local job seekers in Bekasi Regency and the use of information technology and communication in employment services.

Kinerja Tenaga Pendamping Desa Dalam Pembangunan Desa Di Kabupaten Bungo Provinsi Jambi (Studi Di Desa Koto Jayo Dan Desa Tanah Tumbuh Kecamatan Tanah Tumbuh)

This thesis is entitled Implementation of Labour Policy in Bekasi Regency which raises the issue of how the implementation of labour policy in Bekasi Regency is seen from its dimensions and determinants and how to improve labour services in order to increase job opportunities in the Bekasi Regency area. The theory used as an analytical knife in writing this thesis is the theory of policy implementation model according to Muchlis Hamdi (2014) which explains the description of the movement dynamics of policy implementation, namely the dimensions of productivity, linearity and efficiency and the factors that can affect the success of policy implementation, namely the determinants of policysubstance, behavior of implementing tasks, network interactions, target group participation and resources. The method used is a qualitative approach, data collection techniques are interviews, document review, observation, and triangulation. Based on the data and analysis of the implementation of labour policies in Bekasi Regency, seen from the dimensions and determinants, some results are obtained that the implementation of labour policies in Bekasi Regency has been going quite well which can be seen from the achievement of performance targets and SPM increased from 2018 and can meet the target in 2019, the use of funds, infrastructure and implementing officers in supporting employment services is quiteefficient, the contents of employment policies, especially employment services with other policies are aligned , the level of performance and ability to make the initiative of implementing officers is quite good, the interaction of the network of implementing officers is quite harmonious, the participation of the target group both job seekers and company on the implementation of labour policies in Bekasi Regency is quite high, resource support is good, adequate funds,availability of implementers and the availability of equipment for the implementation of labor policies in Bekasi Regency is quite good. However, in order to improve employment services in the context of increasing job opportunities, apartfrom optimizing things that are already going well, it is necessary to make efforts, including the preparation ofstandard operating procedures (SOPs) in overall employment services, drafting Regent regulations to regulate technical implementation guidelines. Bekasi Regency regional regulation number 4 of 2016 concerning Employment, increasing competence for functional officers who are equal to work delivery results, increasing network interaction with employees of the labour inspector Office of West Java Province, increasing the competence and skills of local job seekers in Bekasi Regency and the use of information technology and communication in employment services.

Development and validation of an ultra-high performance liquid chromatography coupled to tandem mass spectrometry method for the quantification of the antileishmanial drug paromomycin in human skin tissue

Bioanalytical assay development and validation procedures were performed to quantify antiprotozoal drug paromomycin in human skin tissue by ultra-high performance liquid chromatography coupled to tandem mass spectrometry. Paromomycin, an aminoglycoside drug, is administered intra-muscularly and used in the treatment of multiple clinical presentations of the neglected tropical disease leishmaniasis. It is currently studied in the treatment of post-kala-azar dermal leishmaniasis, a disease where the Leishmania parasites divide and reside in the skin. We present a target-site bioanalytical method to accurately quantify paromomycin in human skin tissue, with the clinical purpose of quantifying paromomycin in skin biopsies from post-kala-azar dermal leishmaniasis patients originating from Sudan. Enzymatic digestion using collagenase A incubated at 37 °C overnight was employed as homogenization method to produce skin tissue homogenates. Further sample preparation was performed by protein precipitation using trichloroacetic acid and a dilution step. Final extracts were injected onto a C18 analytical column and isocratic heptafluorobutyric acid ion-pair separation and elution were employed. The chromatography system was coupled to a triple quadrupole mass spectrometer for detection. The method was validated in digestion solution over a linear range from 5 to 1000 ng/mL (r2 ≥ 0.9967) with the assay performance of accuracy and precision within acceptable criteria values as stated by the EMA guidelines. Furthermore, matrix effects were observed in human skin tissue and were corrected by the multiple deuterated paromomycin internal standard. No substantial IS-normalized matrix effect was detected along with relatively high sample preparation recovery. Consequently, digestion solution matrix serving as the preparation of calibration standards can be used as surrogate matrix for human skin tissue, which is convenient given the limited availability of control matrix. Finally, paromomycin was accurately quantified in skin of post-kala-azar dermal leishmaniasis patients originating from clinical trials in Sudan.

Marriage Guidance Towards Family Resilience in Aceh: A Study of Islamic Law Philosophy

This paper aims to examine government policies related to marriage guidance in order to establish family resilience with an Islamic legal philosophy approach. This study used a philosophical approach to Islamic law. The data were collected through literature studies and in-depth interviews. The results of the study indicate that government policy has created a marriage guidance program to increase family resilience. The implementation of marriage guidance in Aceh still faces obstacles from the formulation of the implementation model, the preparation of operational standards, methods, techniques, materials, and time allocation, as well as synergies with other related agencies. Marriage guidance is not an absolute requirement for marriage. Both the provincial Ministry of Religion and the City Regency Ministry of Religion do not periodically evaluate the marriage guidance program. The budget from the government is still minimal, and the need for cooperation between institutions causes the implementation of marriage guidance not to run optimally. However, marriage guidance from the perspective of Islamic legal philosophy aims for the benefit of prospective husband and wife, their families and communities, and even the state, therefore, this program is quite essential. Moreover, it is associated with family resilience, meaning that marriage guidance will strongly influence family resilience.

Creative Leadership and Its Relationship to Administrative Performance of Professional Supervisors as seen by Physical Education Teachers in the Province of Babylon

The problem of the research lies in the formulation of the main research question, which is: (What is the relationship between creative leadership and the administrative performance of pedagogical supervisors from the perspective of physical education teachers? As for the research objectives, they were (to elaborate measures of creative leadership and administrative performance for the specialized supervisors. To determine the relationship between creative leadership and administrative performance of specialized supervisors. The second chapter is about the research methodology, the research community and its sample. Two sections were divided into this community: preparatory samples and experimental samples. The researcher used the conditions for the construction and preparation of standards, exploratory experiments, main experiments, and statistical methods. In terms of the third chapter, only the results showing the correlation relationships between the level of creative leadership and the level of administrative performance of subject teachers are presented and discussed. The fourth chapter dealt with the conclusions and recommendations, the most important of which was (the measure of creative leadership for professional supervisors, which consisted of (53) paragraphs. In terms of the measure of the administrative performance consisted of (62) items. The results indicated that the specialized supervisors did not have the required level of creative leadership as well as administrative performance.

방산 사업조정제도 실태 분석 및 개선 방향에 관한 연구

The current defense business adjustment system does not have specific and detailed judgment criteria for overlapping investment and defense efficiency, making it difficult to conduct active and rational business adjustment at the government level. Also, the need to check whether or not it is effective in enhancing the management performance of defense companies is constantly being raised. Therefore, in this study, the management status of the domestic defense industry and the operation status of the defense business adjustment system were analyzed, and a survey was conducted on the business adjustment system targeting defense companies, thereby demonstrating the policy effect of this system on improving the business performance of defense companies. Accordingly, four policy improvement directions were proposed : preset competition types for each business area, preparation of detailed application standards(draft) for business adjustment requirements, improvement of business adjustment procedures and operating organizations, and expansion of organizations eligible for business adjustment applications. It is hoped that this proposal will be of some help to the development of the Korean defense industry and enhancement of competitiveness by being used to improve the defense business coordination system.

Application of Professional Judgement in International Financial Reporting Standards

This essay accesses the use of professional judgements in the preparation and implementation of international financial reporting standards by preparers of financial statements. The article samples some domains of professional judgement in financial reporting contained in the Conceptual Framework for Financial Reporting (2018) and selected IAS/IFRS to demonstrate how judgement is exercised in the preparation and presentation of principles-based standards. The sampled domains or action areas for the exercise of professional judgement are going concern, materiality, accruals accounting, accounting policies, presentation/disclosure, measurements, estimates, recognition/de-recognition, classifications and revenue recognition. From the analyses of these domains, the essay concludes that (i) the exercise of professional judgement is a sine qua non in the implementation of IFRSs; and by extension, the ability to exercise professional judgement is not only the key skill for preparers of PBS-based financial statements; it is also the hallmark of an accounting professional.

Intracellular Absolute Quantification of Oligonucleotide Therapeutics by NanoSIMS.

Antisense oligonucleotide (ASO)-based therapeutics hold great potential for the treatment of a variety of diseases. Therefore, a better understanding of cellular delivery, uptake, and trafficking mechanisms of ASOs is highly important for early-stage drug discovery. In particular, understanding the biodistribution and quantifying the abundance of ASOs at the subcellular level are needed to fully characterize their activity. Here, we used a combination of electron microscopy and NanoSIMS to assess the subcellular concentrations of a 34S-labeled GalNAc-ASO and a naked ASO in the organelles of primary human hepatocytes. We first cross-validated the method by including a 127I-labeled ASO, finding that the absolute concentration of the lysosomal ASO using two independent labeling strategies gave matching results, demonstrating the strength of our approach. This work also describes the preparation of external standards for absolute quantification by NanoSIMS. For both the 34S and 127I approaches used for our quantification methodology, we established the limit of detection (5 and 2 μM, respectively) and the lower limit of quantification (14 and 5 μM, respectively).

Real matrix-matched standards for quantitative bioimaging of cytosolic proteins in individual cells using metal nanoclusters as immunoprobes-label: A case study using laser ablation ICP-MS detection

The persistent lack of adequate matrix-matched reference materials still hinders the quantitative analysis of elements and biomolecules in biological samples by LA-ICP-MS. This fact is especially critical in cell cultures due to their complex matrix. In this work, we propose a novel matrix-matched calibration strategy, which fully mimics the matrix of cultured cells, by using the same cell line of the sample to create laboratory standards. As a model case, the quantitative imaging of two cytosolic proteins (MT2A and APOE) in individual HRPEsv cells was performed by LA-ICP-MS, both in cells subjected to inflammation with cytokine Interleukin-1α (IL-1α) and controls (CT). A single biomarker strategy using Au nanoclusters (AuNCs) as specific antibody labels was employed for the analysis of the selected proteins in individual cells by LA-ICP-MS. HRPEsv cells supplemented with suspensions containing nude AuNCs was employed to generate single-cell laboratory standards (HRPEsv cells@AuNCs). The preparation and characterization of the single-cell laboratory standards by both ICP-MS and LA-ICP-MS were optimized as well as the data treatment protocol required for obtaining the quantitative distribution of the proteins in individual cells. The mass of APOE and MT2A per cell in CT and IL1α−treated HRPEsv cells analysed by LA-ICP-MS using the proposed matrix-matched calibration were successfully corroborated with commercial ELISA kits. In addition, quantitative real time polymerase chain reaction (qPCR) analyses were performed to study the proteins gene expression.

STRATEGY FOR INTEGRATED LAND INFORMATION SYSTEM NETWORK ARRANGEMENTS FOR THE INDONESIAN NATIONAL ARMY

In the era of globalization and information as it is today, and easily accessible data and information management system has become a necessity. Data and information containing ideological, political, economic, socio-cultural, and security aspects are urgently needed to manage national defense policies in order to be able to manage data and information in accordance with the interests of the system, which requires an integrated information network that can collect and manage and regulate the use of data and information. Information according to defense needs. The purpose of this study was to analyze the Strategy for Networking the Integrated Ground Defense Information System, a phenomenological qualitative research method accompanied by data collection techniques of observation, interviews, and documentation studies. The results showed that the TNI AD information system network was not well organized and not integrated where it was found that there were still ranks of the TNI AD that had not been reached by the information system network, then the number of units in the TNI AD was presented, the allocation of internet support for information system purposes was very important. The limited data transmission process, with the non-integration of the information system in the Indonesian Army, resulted in data and information being unable to be shared between data users. The conclusion from this research is that the current Information System Network Arrangement is not optimal, so the Strategy for the Integrated Ground Defense Information System Network Arrangement is carried out with a plan which includes the preparation of an information system title roadmap, the preparation of standards, and the use of independent data, the development of which includes doctrine, personnel, and structure. Organizing and strengthening Infrastructure, which includes the arrangement of LAN and WAN as well as information system network security, including background, objectives, design/methodology/approach, and results/conclusions.Keywords: strategy, structuring, Information System Network, integrated

Preparation of gas standards for quality assurance of hydrogen fuel

This study has developed traceable standards for evaluating impurities in hydrogen fuel according to ISO 14687. Impurities in raw H2, including sub μmol/mol levels of CO, CO2, and CH4, were analyzed using multiple detectors while avoiding contamination. The gravimetric standards prepared included mixtures of the following nominal concentrations: 1, 2, 3–5, 8–11, 17–23, and 47–65 μmol/mol for CO2, CH4 and CO, O2, N2, Ar, and He, respectively. The expanded uncertainty ranges were 0.8% for Ar, N2, and He, 1% for CH4 and CO, and 2% for CO2 and O2. These standards were stable, while that for CO varied by only 0.5% during a time span of three years. The prepared standards are useful for evaluating the compliance of H2 fuel in service stations with ISO 14687 quality requirements.

The use of freeze-dried blood samples affects the results of a dried blood spot analysis

Dried blood spot (DBS) microsampling has several advantages over venous blood sampling. In a clinical validation study of tacrolimus microsampling it was noted that tacrolimus DBS concentrations ([Tac]DBS) were systematically higher than tacrolimus whole-blood concentrations ([Tac]WB). This observation was explored by investigating the effect of using freeze-dried standards (STFD) for [Tac]DBS measurement.For all experiments, both non-frozen whole-blood samples and whole-blood samples that were frozen and thawed (to simulate freeze-drying) of 10 patients were analyzed. Multiple tacrolimus concentrations were measured: 1) [Tac]WB, 2) [Tac]DBS, where 15 μL was volumetrically applied to a pre-punched DBS disk, and 3) [Tac]DBS, where 50 μL was applied before a 6 mm DBS disk was punched from the card. All tacrolimus concentrations were determined independently using STFD and standards made of non-frozen blood spiked with tacrolimus (STSP).In both non-frozen and frozen and thawed whole-blood samples, [Tac]WB measured with STFD appeared similar to [Tac]WB measured with STSP (Ratios 1.061 and 1.077, respectively). In non-frozen samples, the median ratio between the [Tac]DBS measured with STFD, and [Tac]WB measured with STFD (the reference method), was 1.396. When blood was volumetrically applied to the DBS card (to eliminate the effect of the spreading over the filter paper), this ratio was 1.009.In conclusion, when using DBS microsampling to quantify concentrations of analytes, one should be aware that using the commercially available freeze-dried blood samples for the preparation of standards may affect the spreading of blood on the filter-paper, leading to a systematic error in the results.

Reference Materials: gas mixtures to support measurements for climate change studies

This work aims at presenting some activities carried out at INRiM for the development of new reference materials (RMs) of greenhouse gases to support measurements for climate change studies. Since the preindustrial era, greenhouse gases emissions due to human activities have dramatically increased, mostly due to economic and demographic growth and their concentrations in the atmosphere have grown continuously since 2011, reaching highest levels in 2019. The increase of greenhouse gases concentration in atmosphere due to anthropogenic emissions is the main cause of global warming and carbon dioxide (CO2) is one of the main culprits for this phenomenon. In this context, the capability of discriminating between anthropogenic and natural emissions is of utmost importance. The determination of the isotopic composition of CO2 can support the assessment of the uptake of CO2 in the environmental compartments, i.e. atmosphere and hydrosphere, and can help to distinguish natural from anthropogenic carbon in the atmosphere. The possibility to establish metrological traceability of the measurements of CO2 isotopic composition is a key aspect, and greatly relies on the availability of gaseous reference materials having compositions and uncertainties that are fit for purpose. In the past years, INRiM participated in the EMPIR project 16ENV06 SIRS “Metrology for stable isotope reference standards”, and is now taking part in the EMPIR project 19ENV05 STELLAR “Stable isotope metrology to enable climate action and regulation”. INRiM has experience in the preparation of gas standards by primary methods, namely gravimetry and dynamic dilution, which can be applied to the realisation of reference materials of CO2 at known isotopic composition. The present work describes the preliminary results obtained by INRiM in the preparation of pure CO2 RMs and at atmospheric amount fraction. Examples of uncertainty budgets and identification of the uncertainty sources are also given.

Recent Developments for the Deuterium and Tritium Labeling of Organic Molecules

Organic compounds labeled with hydrogen isotopes play a crucial role in numerous areas, from materials science to medicinal chemistry. Indeed, while the replacement of hydrogen by deuterium gives rise to improved absorption, distribution, metabolism, and excretion (ADME) properties in drugs and enables the preparation of internal standards for analytical mass spectrometry, the use of tritium-labeled compounds is a key technique all along drug discovery and development in the pharmaceutical industry. For these reasons, the interest in new methodologies for the isotopic enrichment of organic molecules and the extent of their applications are equally rising. In this regard, this Review intends to comprehensively discuss the new developments in this area over the last years (2017-2021). Notably, besides the fundamental hydrogen isotope exchange (HIE) reactions and the use of isotopically labeled analogues of common organic reagents, a plethora of reductive and dehalogenative deuteration techniques and other transformations with isotope incorporation are emerging and are now part of the labeling toolkit.

The economic costs of voucher markets for public service provision

ABSTRACT Voucher markets where governments subsidise the consumers of public services to give them a free choice of service provider are implemented by public authorities as a means to reap the supposed benefits of competition and choice. Such voucher markets imply economic costs for the public authority in the form of transaction costs, e.g., for preparation of quality standards and information material as well as approval, coordination and supervision of providers. However, voucher markets may also affect the public authority’s production costs through a competitive pressure for increased efficiency and/or by affecting the potential economies of scale for public service delivery. This article shows that in a voucher market without price competition voucher markets increase the public production costs of delivering home help services. Specifically, the larger the share of elderly persons who choose a private provider, the higher the public costs of delivering practical home help.

Diseminasi Standar Makanan, Pengolahan Jus Varian Buah dan Sayur Serta Edukasi Kepada Lansia Hipertensi Di Panti Sosial Tresna Werdha Budi Sejahtera Banjarbaru

Elderly is an age group that is very vulnerable to suffer from hypertension. In addition to medication, blood pressure can be controlled by managing diet and giving fruits and vegetables. The Community Service Team carried out dissemination of food standards, processing of fruit and vegetable variant juices and education for the elderly with hypertension at the Tresna Werdha Budi Sejahtera Social Home Banjarbaru. The purpose of the activity is to provide input to nursing home managers regarding food standards, training and demonstrations of making fruit and vegetable juice variants as well as providing knowledge about eating arrangements for controlling blood pressure in the elderly. Method: Phase I: Dissemination to the management and health workers regarding food standards for the elderly with hypertension. Phase II: Conducting demonstrations of making fruit and vegetable variant juices and training nutritionists and health workers. Phase III: educating the elderly about eating arrangements and measuring blood pressure. The results of the activity: I. The results of the dissemination of suggestions for improving food standards for the elderly in order to control the blood pressure of the elderly at the Tresna Werdha Home., II The results of the juice making demonstration and training: the health workers were very enthusiastic, starting from the explanation from the preparation process to the making process. The results of education and blood pressure examinations found high grade 1 and 2 hypertension conditions. It is not possible to do pre and post-tests to explore the level of knowledge in the elderly. Asking questions directly from what is explained, all the elderly can answer. The preparation of food standards for the elderly with the addition of a composition of vegetables and fruit, the proposal to enter a joint juice drinking program with the elderly, providing continuous mentoring and motivation by nutritionists, are opportunities for the implementation of the MOU/cooperation agreement with the Social Institution.

The development of the library and information systems in the conditions of digital transformation of Ukraine

The scientific and information resources of Ukraine are characterised by biblio-diversity and considerable amount. However, the functional interaction between them is at the insufficient level. As the exemption, we can say about the system of institutional repositories and the collection of scientific periodicals, maintained by the Publishing Service "URAN". It is stated that their interoperability is ensured by the OAI/PMH technology. It is noted that the state approach to the digital transformation of these resources and the organisation of their functional interaction need the adoption of an appropriate target program. The realistic and possible way of this process is the synergetic approach to the consolidation of the Ukrainian segment of scientific knowledge by all stakeholders' cooperative efforts. First of all, this approach requires the intensification of work on the preparation and adoption of regulations and standards that will contribute to the functional transformation of existing information systems into interoperable ones. The further development of works in this direction by creating a domestic analogue of "Books in Print" is determined. The latter one is a single way to access to information about publications of Ukrainian scientists in foreign magazines and the integrated information and search apparatus of the stakeholders. The necessity of the primary research data inclusion to the scientific knowledge environment is pointed out. These data involve the results of the results of experiments, hydro-meteorological observations, sociological surveys, etc. Their using is the prerequisite for the transformation of information institutions from the science and education infrastructural elements into their participants. It is found out that to reduce information entropy we should to involve cognitive-oriented resources, as well as Semantic Web technology. It is remarked that the implementation of the appropriate measures will contribute to the digital transformation of library Ukraine and the formation of the Ukrainian segment of the scientific knowledge global digital environment on this basis.