Ppm Kinetin Research Articles

Effect of salt stress on plant regeneration efficiency in primed and non-primed seed's calli of rice (Oryza sativa L.) variety Swarna.

This study leads with the primed seeds of rice (var. Swarna) with distilled water (D.W.) and various concentrations of Mg(NO3)2 (0-8mM)/Kinetin (0-5ppm) alone or in combination with screen out the regeneration medium induced tolerance level of NaCl. To fulfill the objective, the primed and non-primed rice seeds were inoculated in MS medium supplemented with 30gL-1 maltose + 1gL-1 casein hydrolysate and 2mgL-1 of 2,4-D for callus induction and cultured up to 45days in two sets: one set for regeneration purpose in NaCl-induced regeneration medium and another set was used to study the physiological potentiality of the callus. The 45-day-old calli were transferred into regeneration medium MSR (MS medium for regeneration) (BAP: NAA: Kinetin = 4:1:1) containing NaCl with a concentration range of 0 to 300mM. The number of regenerating calli and shoot regeneration percentage, number of plantlets obtained from one callus, recovery of plantlets from each concentration of NaCl and proline estimation from the leaf of the regenerated plantlets were determined from one set obtained after 45days. The calli obtained from another set after 45days, the frequencies of total and embryogenic calli induction percentage, fresh and dry weights, proline content, nitrate reductase and superoxide dismutase activities were measured. The calli obtained from 2.5ppm kinetin + 4mMMg(NO3)2 primed seeds were showed best result as compared to the other treatments for the above-mentioned parameters in different concentrations of NaCl-induced medium and survive up to 200mM concentrations of NaCl.

Pengaruh Kombinasi BAP (Benzyl Amino Purine) dan Kinetin Terhadap Multiplikasi Tunas Tanaman Tebu (Saccharum officinarum L.) Varietas AAS Agribun

Sugarcane (Saccharum officinarum L.) is a plantation commodity crop and raw material for making sugar. The need for sugar increases every year so it is necessary to provide superior sugarcane seeds in large quantities in a relatively short time. This can be done by propagating using the plant tissue culture method. The research method used was an experimental method using a combined Completely Randomized Design (CRD) with 4 replications and 8 treatments consisting of: Control (P0); 0.5 ppm BAP + 0 ppm kinetin (P1); 1 ppm BAP + 0 ppm kinetin (P2); 2 ppm BAP + 0 ppm kinetin (P3); 0 ppm BAP + 0.1 ppm kinetin (P4); 0.5 ppm BAP + 0.1 ppm kinetin (P5); 1 ppm BAP + 0.1 ppm kinetin (P6); and 2 ppm BAP + 0.1 ppm kinetin (P7). The data then explained the variations and then continued with the DMRT test at the 5% level. The results showed that treatments P3 and P7 showed the best results at the time of shoot emergence. The P3 treatment also showed the best results in the number of shoot (8.25) and number of leaves (16.50). The P7 treatment showed the highest shoot yield (4.58). Keywords: sugarcane, BAP, kinetin, shoot multiplication

The Study of Exogenous Auxin and Cytokinins in Embryogenesis and Fiber Genes Expression during In Vitro Regeneration of Cotton (Gossypium hirsutum L.)

The efficiency of regeneration media, based on the morpho-cytochemical response and embryogenesis gene expression of widely cultivated cotton in Indonesia (Gossypium hirsutum L.), needs to be studied further. This study's objectives are to identify the effect of 2,4-D and IBA hormones on the morphology and induction of cotton callus; to determine the effect of regeneration media on the morphology, cytochemical, and gene expression of cotton plant during embryogenesis. This study was conducted by inducing cotton callus on MS0 medium, MS Basal + 0.1 mg/L 2,4-D and MS Basal + 0.1 mg/L IBA. The best callus induction results were regenerated on the regeneration media, namely M1 (MS Vitamin + 0.01 ppm 2,4-D + 0.3 ppm IBA + 0.5 ppm Kinetin), M2 (MS Vitamin + 0.01 ppm 2,4-D + 0.5 ppm IBA + 0.5 ppm Kinetin), M3 (MS Vitamin + 0.00 ppm 2,4-D + 0.3 ppm IBA + 0.5 ppm Kinetin), and M4 (MS Vitamin + 0, 00 ppm 2,4- D + 0.5 ppm IBA + 0.5 ppm Kinetin). The embryogenesis gene expression was observed. The best morphological results for callus induction were obtained on MS Basal + 0.1 mg/L 2,4-D medium with a compact and white callus, indicates high embryogenic and regeneration ability. The highest percentage of regenerated callus parameters was found in M2 media and further confirmed by cytochemical characterization. Embryogenesis genes expressed were GhSERK1, GhSERK2, LEC, GhWUS, and GhWOX11. Other fiber-related genes GhMYB25-like, GhHD-1, and F3H were also expressed during the embryogenesis.

Effects of Low-Dose Kinetin, 2,4-D and Monochromatic Light Conditions on Flavonoid Content in Callus Culture of Dioscorea esculenta

The callus culture of Dioscorea esculenta is a potential strategy to produce efficacious bioactive compounds in multiplied production. It can be carried out by applying plant growth regulators (PGR) and environmental modification, including light-emitting diodes (LEDs) exposure. This study aims to investigate the impact of PGR and the stimulatory effect of LEDs on flavonoid profiles in D. esculenta callus culture. The study was conducted using combination variations of 2,4-D (0.5 and 1 ppm), kinetin (0.5 and 1 ppm), and LEDs stimulatory conditions (light and dark). The results showed that flavonoids are the dominant compounds found in lesser yam cultures, which reached more than 78 % in all treatments-specifically, adding 0.5 ppm kinetin and 0.5 ppm 2,4-D to D. esculenta callus cultures significantly increased flavonoids production compared to single PGR treatment. This shows there is a synergistic activity induced by 2,4-D and kinetin. Furthermore, monochromatic light exposure also significantly affects flavonoid production. Analysis of 5 main flavonoids, isoflavones, flavanones, kaempferols, quercetin, and epigallocatechin, proved that monochromatic light significantly increased flavonoids production compared to callus cultures were kept in a growth room under 24 °C temperature in the dark condition. This study confirmed that equally combining 0.5 ppm of 2,4-D and 0.5 ppm of kinetin under monochromatic LEDs increases flavonoid production in D. esculenta callus cultures. Further research must be conducted to describe how the bioactive compound synthesis signaling pathway involving PGR and monochromatic LEDs improves plant quality in the pharmaceuticals’ role. HIGHLIGHTS Kinetin and 2,4-D at the same concentration of 0.5 ppm is an optimal combination to increase secondary metabolite production in esculenta callus culture Combining kinetin and 2,4-D at low concentrations significantly increased flavonoids, especially kaempferol, isoflavone, quercetin, flavanone, and epigallocatechin in esculenta callus culture Light condition significantly affects the production of flavonoids, especially kaempferol, isoflavone, quercetin, flavanone, and epigallocatechin There is evidence that kinetin and 2,4-D under light conditions work in a synergic effect on the biosynthesis of secondary metabolite compounds in esculenta callus cultures GRAPHICAL ABSTRACT

Effect of Varied Levels of Gibberellin, Anti-Gibberellin and Cytokinin on Growth and Tuberization in Potato

Plant hormones play a significant role in potato tuberization. While Gibberellins inhibit tuberization, anti-gibberellins (PBZ and CCC) and cytokinins promote tuberization in potato. The experiment demonstrated the clear roles of these hormones in facilitating tuberization in potato. Treatment with 25 ppm GA significantly increased plant height (44.56 cm) compared to the control (32.22 cm), while CCC, a Gibberellins (GA) inhibitor, reduced the plant height by inhibiting GA-induced stem elongation. Hormonal treatments affected the number of nodes, leaves, branches, SPAD readings, stem girth, root length and root weight with significantly higher values observed compared to the control. 50 ppm GA showed the highest number of stolons per plant (6.33), but surprisingly resulted in a few tubers per plant (1.7) compared to the control (1.8). However, both 5 ppm and 10 ppm BAP led to significantly more tubers per plant (2.1 and 3.2 respectively), as did 5 ppm and 10 ppm Kinetin (2.8 and 2.6 respectively). Regarding tuber fresh weight, all treatments except CCC and higher concentration of kinetin showed significantly higher fresh weight compared to control. In summary, 50 ppm Gibberellins increased the stolon number, while 10 ppm BAP increased the tuber number and fresh weight in potato

Evaluation of Stevia rebaudiana Leaf-Axillary Shoot Formation, Cultured in MS Medium Supplemented with IAA-BAP and MS Medium Supplemented with Kinetin

Stevia rebaudiana leaves can be used as a sweetener alternatives because they contain steviol glycoside derivative compounds, including steviosides and rebaudioside-A. Propagation of Stevia is more optimally carried out using in vitro culture when compared to conventional propagation through seeds or cuttings. This study aimed to evaluate the formation and growth of Stevia shoots and leaves in MS medium containing a mixture of IAA and BAP with MS medium containing kinetin only, as well as evaluating the use of a liquid medium containing kinetin. Stevia was initiated from apical shoot then grown in MS medium containing a mixture of IAA and BAP with MS medium containing kinetin only. Stevia was subcultured every 4 weeks. Several parameters measured were number of axillary shoots and number of leaves. It was transferred into a liquid medium for 7 days. The results showed that the formation and growth of axillary buds and leaves at the initiation stage were better in medium containing IAA and BAP compared to medium containing single hormone kinetin. At the stage of shoot multiplication and maintenance, cultivation in semi-solid medium containing kinetin showed more leaves and axillary shoots compared to that cultivated in semi-solid medium with the addition of IAA and BAP. Plants acclimatized in liquid medium supplemented with 1 ppm kinetin showed fast plant growth but were not accompanied by sturdy stem growth. The presence of brownish color on certain parts of the plant such as in some leaves and stems was also observed.

Organs formation on Jabon trees (Anthocephalus cadamba (Roxb) Miq) by in-vitro: treatment of various iba-kinetin combination

The objective of this study aimed to determine the concentration of the combination Indolebutiric acid (IBA) - Kinetin suitable for the formation of roots of the Jabon plant (Anthocephalus cadamba (Roxb) Miq) in vitro. This research is a follow-up study of the initiation and propagation of in vitro shoots of Anthocephalus macrophyllus (Roxb) Havil). The study was conducted at the Forestry Biotechnology Laboratory, Department of Forestry, Faculty of Forestry, University of Tadulako from February 2018 to August 2018. The study was arranged in a completely randomized design with the addition of IBA and Kinetin to Murashige and Skoog basic medium in various concentrations as treatments. Five concentration levels of combinations of IBA + Kinetin were studied, namely: H1(0.1 ppm IBA + 3 ppm Kinetin), H2 (0.5 ppm IBA + 1 ppm Kinetin), H3 (1.0 ppm IBA + 0.5 ppm Kinetin), H4 (1.5 ppm IBA + 0.3 ppm Kinetin), H5 (2 ppm IBA + 0.1 ppm Kinetin). The experimental results showed that the combination of 1 ppm IBA + 0.5 ppm Kinetin concentration could promote the formation of Anthocephalus cadamba roots the best compared to other treatments. Treatment of 0.1 ppm IBA + 3 ppm kinetin and treatment of 0.5 ppm IBA + 1 ppm kinetin did not respond to root formation, but encouraged the formation of the best shoots of Anthocephalus cadamba.

Response of Biofertilizers and Kinetin on Growth and Yield Parameters of Summer Blackgram (Vigna mungo L.)

The field experiment on blackgram was carried out in summer, 2022 at the Crop Research Farm in the Department of Agronomy. The treatment consisted of three levels of biofertilizer no seed inoculation, Phosphate solubilizing bacteria (20 g/kg seeds), Rhizobium (30 g/kg seeds), three levels of kinetin (20, 30 and 40 ppm) foliar spray and a control. The experiment was set up using a Randomized Block Design with ten treatments and three replications. The variety black gold of black gram was sown in Zaid 2022. The results of the experiment revealed that foliar application of 40 ppm kinetin along with biofertilizers recorded significantly increased growth parameters viz., plant height (33.94 cm), number of branches (8.13), number of nodules per plant (36.80), plant dry weight (4.47 g/plant), crop growth rate ( 8.23 g/m2/day), relative growth rate (0.10 g/g/day). Same treatment also resulted in boosting yield and yield attributes viz., number of pods per plant (27.50), number of seeds per pod (8.07), test weight (40 g), seed yield (0.71 t/ha), stover yield (1.82 t/ha), harvesting index (28.09 %). In terms of gross returns (67450.00 INR/ha), net returns (44885.00 INR/ha), and the B:C (1.99), this treatment also demonstrated its favorable effects.

Shoots and Roots Induction of Garlic on Different Composition of Plant Growth Regulators and Photoperiod

Plant Growth Regulators and photoperiods will affect callus morphogenesis from garlic bulbils. The study aimed to obtain the best interaction between media composition and photoperiod for shoots and root induction of the garlic callus. The research has two-stage. The first stage consisted of two factors. The first factor is the combination of PGR for shoots induction which consists of three combinations, i.e., 0.1 ppm NAA + 1 ppm BAP, 2 ppm NAA + 2 ppm BAP, and 0.4 ppm 2,4-D + 2 ppm kinetin. The second factor is photoperiod which consists of 10, 16, and 22-h light conditions. The second stage is the root induction in different PGRs (2 ppm IBA and 0.2 ppm NAA + 2.25 ppm BA) under continuous dark conditions. The results of the first stage showed that the PGR produced the most shoots was 0.1 ppm NAA + 1 ppm BAP. After four weeks, the frequency of an explant emerging during the shoot reached 64% on media 0.1 ppm NAA + 1 ppm BAP. The treatment MS media supplemented with 0.1 ppm NAA + 1 ppm BAP at 16-h photoperiods increased the growth of shoots per explant in 11-15 shoots and produced the most extended shoot length. The result of the second stage showed that the number of roots on 2 ppm IBA is the same as NAA 0,2 ppm + BA 2,25 ppm, but the treatment 2 ppm IBA has the highest root number in three months.

Pengaruh Kinetin Pada Pertumbuhan Kecambah Brokoli Hibrida (Brassica oleracea Var. Green Magic) Dan Kandungan Sulforafan Pada Kultur In Vitro

This study aims to observe the growth in the sprouting phase and to observe the sulforaphane content in the growth stages of broccoli sprouts treated with kinetin on MS media. The research was conducted at the Laboratory of Biotechnology, Faculty of Agriculture, Sam Ratulangi University and the Laboratory of Department of Chemical Engineering, State of Malang. The study used a Completely Randomized Design (CRD) with 4 treatments and 9 replications, nam ely without kinetin, 0.5 ppm kinetin, 1 ppm kinetin, 1.5 ppm kinetin. The variables observed were the time of sprout formation, sprout height, sprout weight, sulforaphane content. The results of this study showed that the sulforaphane content was not affected by the height and weight of the sprouts but the kinetin concentration, the higher the concentration given, the higher the sulforaphane content.

Investigate growth behavior and chemical constituents of cordyline plants under kinetin spraying

At greenhouse of National Research Centre, Dokki, Giza, Egypt. A pot experiment was carried out to evaluate the effect of Kinetin spraying on growth and chemical constituents of cordyline plants. The present study shows a considerable difference in the growth parameters when treated with kinetin concentrations of 0, 100, 150 and 200 ppm compared with the control plants. It was found that, increasing levels of kinetin from zero to 200 ppm increased plant height, root length, stem diameter as well as leaves number, anthocyanin pigments and K%. On the other hand, application 200 ppm recorded decreased in nitrogen and phosphor percentage compared with the control. While application of 100 ppm kinetin significantly increased chlorophyll and protein content compared with the control plants.

Callus induction from tuber of lesser yam (Dioscorea esculenta) on MS media supplemented by 2,4-D and kinetin

Lesser yam contains active pharmaceutical compounds. Secondary metabolic production of lesser yam plants takes time because of the long harvest period. Callus induction can be used to acquire active compounds in relatively short periods. This study aimed to determine the optimum concentration of 2,4-D and kinetin combination in inducing lesser yam callus in light and dark conditions. The research design used was a two-factor randomized block design, namely the combination of PGR (2,4-D 1 ppm + kinetin 1 ppm, 2,4-D 0.5 ppm + kinetin 1 ppm, 2,4-D 1 ppm + kinetin 0.5 ppm, 2,4-D 0.5 ppm + kinetin 0.5 ppm) and lightness conditions (light and dark). The observed parameters included callus appearance time, percentage of callus formation, and callus color and texture. The results showed that the combination of 0.5 ppm 2,4-D + 0.5 ppm kinetin in light conditions indicated the fastest callus time. Meanwhile, the percentage of explants with the highest callus was shown in all treatments in dark conditions by 100%. The calluses produced in light conditions were generally green with a friable texture, calluses in dark conditions were generally white and had friable texture.

Magnetite Nanoparticles Different Sizes Effectiveness On Growth And Secondary Metabolites In Ginkgo Biloba L. Callus

This study investigated the effects of magnetite nanoparticles (MNPs) various sizes (10.77, 20.5, and 29.3 nm), and different concentrations (0.5, 1, and 2 ppm) on the growth and production of active constituents in Ginkgo biloba L callus. The best medium for callus growth and proliferation of Ginkgo biloba L was MS medium supplemented with 1 ppm naphthalene acetic acid (NAA), 1 ppm Kinetin, and 0.5 ppm MNPs with a size of 10 nm, which gave the maximum fresh weight of callus and percentage increase in fresh weight after two subcultures. The maximum accumulation of ginkgolide A and bilobalide were achieved when callus was elicited by MNPs 10 nm at 2 ppm. This study also focused on estimating phenolic compounds and scavenging free radicals (antioxidant activity) for their indirect effect on active constituents production. Also, MNPs different sizes had a clear effect on the number of phenolic compounds, where the maximum values were recorded with (2 ppm + size 10.77 nm). The major components were quercetin, kaempferol, p-coumaric acid, rutin, and caffeic acid. Moreover, the highest percentage of antioxidant activity (DPPH) was recorded when callus treated with (2 ppm + size 10.77 nm) which reached 77.29 %. This study highlights the importance of MNPs for the production of ginkgolide A and bilobalide from Ginkgo biloba L. and focusing on the relationship between their size and concentration. Besides, to provide a good and permanent source of active substances of medical and industrial importance on a large scale and at the lowest cost.

Embryogenic Callus Induction of Todolo Toraja Coffee Leaf Cells (Coffea arabica Var. Typica) with the Addition of 2, 4-DichlorophenoxyaceticAcid (2, 4-D) and Furfurylaminopurine (Kinetin) in Vitro

Somatic embryogenesis is a way to produce large numbers of seeds according to the parent, so it is very profitable in the effort to conserve Arabica coffee, which has reduced number of trees and is not productive. This study uses Todolo arabica coffee (Coffea arabica Var. Typica) which is the oldest commercial coffee found in Toraja, South Sulawesi and is threatened with extinction. This study aims to obtain the best composition in administering zpt 2,4-D and kinetin in forming embryogenic callus from explants of arabica coffee leaves. This research was conducted at the Tissue Culture Laboratory of the Faculty of Mathematics and Natural Sciences, Hasanuddin University. The experimental range used was a completely randomized design with 2 factorials, namely the concentration of 2,4-D 1 ppm, 2 ppm, and 3 ppm and kinetin concentration of 0.1 ppm, 0.5 ppm and 1.0 ppm so that there were 9 treatments with 3 replications. The parameters observed were callus formation time, callus structure, and callus color. The results showed that the fastest time for callus appearance was 17.00 HSK with 1 ppm 2,4-D and 0.5 ppm kinetin. The callus texture formed is a crumb-textured callus with several color variations that are formed.

The Direct Organogenesis In Local Clones Of Patchouli Plant( Pogostemon cablin Benth)InVitro

Background and Objective: The purpose of this research is to get to be able to identify local patchouli plants that are able to produce the best plantlets in many concentrations of BAP. Materials and Methods: The research conducted in Laboratory of Tissue Culture Faculty of Agriculture, Andalas University from May until November 2019. The research material used in this study was the accession of patchouli plants in West Sumatra, namely: Situak and method of research is completely randomized design.Results: Concentrations of 0.5 ppm kinetin + 0.5 ppm BAP, 0.5 ppm kinetin + 1 ppm BAP and 1 ppm kinetin + 1 ppm BAP can stimulate organogenesis (bud) in vitro

Organogenesis optimization in Dendrocalamus asper

Plant tissue culture offers the potential for rapidly increasing selected bamboo clones for conservation and reforestation. Bud break is important for the successful micro propagation in bamboo. An organogenesis optimization protocol is described for Dendrocalamus asper. Nodal explants containing auxiliary buds from 8-10 years old field grown clumps of D. asper were established in Murashige and Skoog’s (MS) medium supplemented with different concentrations of phytohormone 6-benzyelamino purine (BAP) and kinetin. The maximum bud breaking was observed in 6 ppm BAP concentration. Shoot proliferation was found maximum in MS media supplemented with combinations of BAP and kinetin (4.0 ppm BAP, 0.5 ppm kinetin and 4.0 ppm BAP, 1.0 ppm kinetin) after subcultureing the explants.

PENGARUH JENIS EKSPLAN DAN KOMBINASI ZAT PENGATUR TUMBUH (ZPT) TERHADAP INDUKSI KALUS Begonia bimaensis Undaharta & Ardaka SECARA IN VITRO

Begonia bimaensis Undaharta & Ardaka is a potential ornamental plant, and currently known only from one population in Sumbawa. Propagation programs, both conventional and in vitro culture are necessary to ensure its conservation. The aim of this research is to observe the effects of explant types and plant growth regulator combination (2,4-D and kinetin) in inducing callus from B. bimaensis leaf in vitro. Callus induction was initiated from three parts of leaf explant, namely petiole, leaf base, and leaf lamina. The explants were planted on Murashige & Skoog (MS) medium with addition of 2,4-D and kinetin. Concentrations of 2,4-D were 0, 0.5, and 1 ppm, while kinetin concentrations were 0, 1, and 2 ppm. Each treatment was replicated 10 times. Results showed that leaf base was the best explant used for callus induction. Medium D1K2 (MS + 1 ppm kinetin) showed the fastest time for callus induction that was at 20 days after planting. The highest percentage of callus production (100%) was found on D1K3 (MS + 2ppm kinetin); D2K2 (MS + 0.5ppm 2,4-D + 1 ppm kinetin); D2K3 (MS + 0.5ppm 2,4-D + 2ppm kinetin) and D3K2 (MS + 1ppm 2,4-D + 1ppm kinetin).

The Ability of Pineapple Callus Regeneration (Ananas comosusL.) from Sipahutar North Sumatra Indonesia with in Vitro Culture

Pineapple (Ananascomosus L.) from Sipahutar is an herbaceous plant from Sipahutar District. Pineapple is canning industry sourced from Sipahutar pineapple has the opportunity to be developed in North Sumatra. Propagation through in vitro culture is an alternative method to solve this problem. The purpose of this research was to determine the regeneration ability of pineapple callus derived from callus induction with the addition of 2,4-D 1 ppm and BAP 1 ppm. The callus were regenerated using the addition of Kinetin (0; 0.5; 1; 1.5 ppm). Each treatment was carried out 4 times. The results showed: 1) the fastest time of emergence of shoots (5 days) came from 0.5 ppm, 2) formed the most shoots (8 shoots) resulting from the addition 0 ppm and 0.5 ppm, 3) the most number of leaves (7.75 leaves) resulted from light treatment with the addition kinetin 0.5 ppm and 1 ppm, 4) the longest leaf length (4.44 cm) from dark treatment with kinetin 0.5 ppm, 5) the highest plant height (5.32 cm) from dark treatment with 0.5 ppm kinetin. This shows that the concentration of 0.5 ppm kinetin with the dark treatment is the best concentration to stimulate the formation of pineapple shoots.

Analysis of Secondary Metabolites of Shoot, Callus Culture and Field Plant of Chrysanthemum morifolium Ramat

The chrysanthemum plant (Chrysanthemum morifolium Ramat.) contains many secondary metabolites such as flavonoids and various volatile compounds that can be utilized as drugs. Tissue culture can be an alternative to enhance the production of certain secondary metabolite. The study aimed to determine the types of secondary metabolites that contained in shoot culture, callus and field plants of C. morifolium. The research method was exploration in the laboratory to analyze and compare the content of secondary metabolite from shoot culture, callus and field plants of C. morifolium. Callus was induced by explants of C. morifolium plantlet stems and leaves respectively on MS medium with an addition of 3 ppm 2,4-D + 2 ppm kinetin and 4 ppm 2,4-D. For shoot culture, single nodule explants with one leaf were planted on MS media with the addition of 1 ppm BAP. The secondary metabolite compouds were analized and identified by GC-MS (Gas Chromatography-Mass Spectrometry). The results showed that various types of secondary metabolites contained in shoot culture, callus and field plants of C. morifolium. In callus culture from leaf explants, four compounds from groups of alcohol, acetic acid and organosilicon were identified, whereas in callus culture from stem explants were identified eight compounds from aldehydes, esters, alkanes, and carboxylic acids group. In the shoot culture, nine compounds of alcohol, ketone, aldehyde, cycloalkane and organosilicon group were identified, while in the field plants five compounds were identified from the cycloalkanes, ketones, organoborones and organosilicon group. Some detected compounds have a potential as precursors of alkaloid, phenolic, and flavonoid.Keywords: chrysanthemum, culture, shoots, callus, secondary metabolites.

Mikropropagasi Rubia akane Nakai dari Eksplan Tunas Pucuk dan Buku

Rubia akane Nakai is a medicinal plant which contains antraquinone and natural dyes producing plants because it contains alizarin and purpurin. Propagation of this plant is usualy done through seeds. One alternative to mass propagation which is not seasonal dependent is by tissue culture techniques. The purpose of this study was to conduct R. akane micropropagation on MS media containing cytokinin Kinetin or BAP respectively at 0 (control); 0,5; 1; and 2 ppm using shoot tips and nodes explants. The explants used were shoot containing 1 bud and shoot containing 2 buds, and single node. The results showed that 8 weeks after planting, the highest shoots were produced from MS media without cytokinins from shoot containing 1 bud explants that were 8,36 cm, the highest total number of leaves was produced on MS media with the addition of 0,5 ppm Kinetin at 45,86, the number the highest internodes was obtained from shoots containing 2 buds on MS media with the addition of 0,5 ppm BAP which is 4,72 segments, the highest number of lateral shoots was obtained from shoots containing buds planted on MS media which were 4,57 shoots, and the highest number of roots was obtained from shoot containing 1 shoot planted on MS media that was 9,14. The survival rate of planlet in the greenhouses from MS media containing BAP (83,33%)was higher compared to planlets derived fromMSmedia containing Kinetin (60,42%).