Organs formation on Jabon trees (Anthocephalus cadamba (Roxb) Miq) by in-vitro: treatment of various iba-kinetin combination

Abstract

The objective of this study aimed to determine the concentration of the combination Indolebutiric acid (IBA) - Kinetin suitable for the formation of roots of the Jabon plant (Anthocephalus cadamba (Roxb) Miq) in vitro. This research is a follow-up study of the initiation and propagation of in vitro shoots of Anthocephalus macrophyllus (Roxb) Havil). The study was conducted at the Forestry Biotechnology Laboratory, Department of Forestry, Faculty of Forestry, University of Tadulako from February 2018 to August 2018. The study was arranged in a completely randomized design with the addition of IBA and Kinetin to Murashige and Skoog basic medium in various concentrations as treatments. Five concentration levels of combinations of IBA + Kinetin were studied, namely: H1(0.1 ppm IBA + 3 ppm Kinetin), H2 (0.5 ppm IBA + 1 ppm Kinetin), H3 (1.0 ppm IBA + 0.5 ppm Kinetin), H4 (1.5 ppm IBA + 0.3 ppm Kinetin), H5 (2 ppm IBA + 0.1 ppm Kinetin). The experimental results showed that the combination of 1 ppm IBA + 0.5 ppm Kinetin concentration could promote the formation of Anthocephalus cadamba roots the best compared to other treatments. Treatment of 0.1 ppm IBA + 3 ppm kinetin and treatment of 0.5 ppm IBA + 1 ppm kinetin did not respond to root formation, but encouraged the formation of the best shoots of Anthocephalus cadamba.