In the current situation of endemicity of highly pathogenic avian influenza (HPAI) in Egypt, improving the biosecurity of poultry production has become essential to the progressive reduction the incidence of the disease. A significant proportion of the Egyptian commercial poultry system consists of small-scale poultry producers operating with low to minimal biosecurity measures. An investigation was conducted into the level of adoption of standard biosecurity measures of the small-scale commercial chicken growers, including both farm- and home-based commercial production, input suppliers and other actors along the meat chicken value chain in Fayoum, Egypt. The study which used direct observations and group discussions of nearly 160 participants and structured interviews with 463 respondents, assessed biosecurity implementation to improve management practices and ultimately to control and prevent highly pathogenic avian influenza (HPAI). The survey found that overall, biosecurity measures are rarely implemented in small-scale commercial poultry production units. Compliance with recommended biosecurity practices did not greatly vary from home-based to farm-based commercial production. Furthermore, serious risk practices were identified, such as unsafe disposal of poultry carcasses and potential disease spread posed by poor biosecurity measures implemented during vaccination. HPAI control measures have been ineffective due to limited cooperation between public and private sector, aggravated by the unpopular measures taken in the event of outbreaks and no compensation paid for incurred losses. Outreach and biosecurity awareness raising initiatives should be specifically developed for small-scale producers with the objective of improving general poultry management and thus preventing HPAI and other poultry diseases.