Positive Rates Of E-cadherin Research Articles

Effects of Regional Arterial Infusion Chemotherapy Combined With Laparoscopic Resection on the Prognosis of Colorectal Cancer

To explore the effect of regional arterial infusion chemotherapy combined with laparoscopic surgery on the prognosis of patients with colorectal cancer and the serum interleukin-8, insulin-like growth factor binding protein-3, blood vascular endothelial growth factor and E-cadherin. From January 2019 to January 2020, 122 cases of colorectal cancer patients who planned to undergo laparoscopic surgery in our hospital were selected as the research objects and the research objects were divided into observation group and control group according to the random principle, with 61 cases in each group and control group. The patients in the group received laparoscopic surgery and the patients in the observation group received regional arterial infusion chemotherapy before operation. The total incidence of complications after operation was compared between the two groups and the serum tumor marker levels before and after operation was compared between the two groups, including interleukin-8, E-cadherin, insulin-like growth factor binding protein-3 and vascular endothelial growth factor; 3 y after operation, the comparison survival rates of patients in the two groups. The total incidence of postoperative complications in the observation group was 8.20 %, the interleukin-8 level was 92.16±11.1 pg/ml, the positive rate of E-cadherin was 37.70 % and the insulin-like growth factor binding protein-3 level was 4.08±0.72 ng/ml. The overall incidence of complications in control group was 14.75 %, the interleukin-8 level was 93.08±11.54 ng/ml, the positive rate of E-cadherin was 34.43 % and the insulin-like growth factor binding protein-3 level was 4.11±0.74 ng/ml. There was no statistically significant difference between the two groups (p>0.05). The vascular endothelial growth factor level in the observation group was 129.54±16.98 pg/ml, which was significantly lower than that in the control group (192.72±17.25 pg/ml) and the difference was statistically significant (p<0.05). During the 3 y follow-up, the difference in survival rate between the two groups was statistically significant (p>0.05). Regional arterial infusion chemotherapy combined with laparoscopic resections is safe and feasible because it can reduce the vascular endothelial growth factor level in colorectal cancer patients and improve the 3 y survival rate of patients without increasing the incidence of surgical complications and inflammation.

PO-238 Clinicopathological significance of EMT markers in thymic epithelial tumours

IntroductionThymic epithelial tumours (TETs) are the relatively rare tumours originated from thymus. TETs are histologically categorised according to the WHO classification based on the morphology of epithelial tumour cells and proportion of lymphocytic involvement. Epithelio-mesenchymal transition (EMT) has reported to play pivotal roles in tumour progression including invasion/metastasis, drug resistance, and cancer stemness. However, the precise clinicopathological associations with EMT characteristics in TETs remain to be elusive. Herein, we have examined EMT markers in TETs integrated with their clinicopathological information.Material and methodsA total of 109 patients with TETs were surgically resected between 2002 and 2017 in our institution. Tissue samples were collected from the paraffine-embedded tumour blocks of these patients. The 2 mm cores in diameter of the most representative areas of the tumours were selected by the pathologist and assembled to make tissue microarrays. Immunohistochemical (IHC) stainings for E-cadherin (E) and vimentin (V) were performed. P63 and pan-cytokeratin were additionally stained to detect epithelial tumour cells. The expressions of these molecules were scored and quantified to categorise the activation level of EMT into three groups based on the combination of E and V levels: Full; E-V+, Partial; E+V+ or E-V-. Null; E+V-. For statistical analyses, chi-square tests and Cox hazard models were applied. P-value less than 0.05 was considered significant.Results and discussionsThe median follow-up time was 54.3 months (range, 0.3–178.7). Predominant WHO histological subtypes were as follows: A;8, AB;18, B1;30, B2;30, B3;6. C;15, others;2. Pathologic stage distributions by TNM classfication were as follows: 1;80, 2;2, 3;13, 4a;7, 4b;4. The positive rates of E-cadherin or vimentin expression in epithelial tumour cells were 54.2% and 57.9%, respectively. EMT activation was significantly exhibited in indolent predominant WHO subtype, and in the early stage of pT factor and the Masaoka classification (p=0.0005, 0.0493, and 0.0366, respectively). Multivariate analyses of overall survival time including pT, pN, pM, age, and EMT levels showed that pT factor was significantly prognostic (p=0.0218), but not for EMT.ConclusionEMT activation mechanisms in TETs were inversely correlated with their histological subtype and the primary tumour progression. Further studies are necessary to elucidate tumour progression mechanisms in TETs.

Patients treatment with neuroglioma by teniposide and semustine and its influence on Twist and E-cadherin expression

This study focuses on curative effects of teniposide combining with semustine on patients with neuroglioma and the influences on the expression of Twist and E-cadherin in tissue. Sixty-eight patients with neuroglioma taking operation in our hospital were divided into two groups randomly. Single radiotherapy was given to 34 patients in group A, and teniposide (VM-26) and semustine (Me-CCUN) were added to radiotherapy for 34 patients in group B. Then, curative effects, survival rate, living quality and adverse reaction rate after operation were compared between two groups. Moreover, the difference in positive expression rate of Twist and E-cadherin before and after treatment between two groups was analyzed by immunohistochemistry. Results: In group B, the effective rate of treatment was 88.2%, and the disease control rate was 70.6%, higher than 52.9% and 32.4% in group A with statistical significance (P<0.05). Moreover, the survival rate in three years of group B was 44.1%, and the score of living quality was 67.11±4.32, and also higher than 23.5% and 63.79±4.53 in group A with statistical significance (P<0.05). However, the difference between two groups in adverse reaction rate has no statistical significance (P>0.05). In addition, the difference in positive expression rate of Twist and E-cadherin between group A and group B has no statistical significance before treatment (P>0.05). After treatment, however, the positive rate of Twist in group B is lower than that in group A, while the positive rate of E-cadherin is higher. Both differences have statistical significance (P<0.05). Chemotherapy of VM-26 combining with Me-CCNU can inhibit Twist expression and improve the expression rate of E-cadherin to help improving the curative effects and living quality and increasing survival rate.

Expression and significance of Snail and E-radherin in renal clear cell carcinoma

Objective To study the expression of transcriptional factor Snail and adhesion factor E-cadherin in renal clear cell carcinoma (RCCC) and evaluate their correlation with pathological grading,clinical stage,invasion and metastases of RCCC.Methods The expression of Snail and E-cadherin proteins was detected by using immunohistochemical streptavidin-peroxidase method in 69 cases of RCCC tissue,58 cases of the para-cancerous mucosa and 10 cases of normal renal tissue.Results The positive rate of Snail in RCCC was 82.7％,which was significantly higher than that in para-cancerous mucosa (43.1％,P ＜0.01 ).The positive rate of E-cadherin in RCCC was 31.9％,which was significantly lower than that in para-cancerous mucosa (91.5％,P ＜0.01 ).The expression of E-cadherin and Snail was significantly related with the differential degrees,clinical stage,depth of tumor invasion and distant metastasis ( P ＜ 0.05 ).There was a negative correlation between the expression of E-cadherin and Snail in RCCC.Conclusion The over-expression of Snail and decreased expression of E-cadherin might be important biological markers for invasion and metastasis of RCCC.The combined detection of E-cadherin and Snail has fas-reaching significance on predicting the invasion and metastasis of RCCC. Key words: Renal cell carcinoma; Snail; Cadherin

Effects of CDH1 gene promoter methylation on expression of E-cadherin and beta-catenin and its clinicopathological significance in colon carcinoma

To investigate the relationship between methylation of the CDH1 gene promoter on the expression of E-cadherin and β-catenin, and to evaluate the correlation with clinicopathological characteristics of the colonic carcinoma. Methylation specific PCR (MSP) was used to detect CDH1 gene promoter methylation in the cancer tissue, adjacent tissues and normal tissues in 68 patients. The expression of E-cadherin and β-catenin was determined by immunohistochemistry staining. The positive rate of CDH1 gene promoter methylation was 32.4% in adjacent tissues and 57.4% in cancer tissue, while no detectable methylation was found in all the normal tissues. The difference was statistically significant. The positive rate of E-cadherin was 92.6% in the normal tissues, 66.2% in the adjacent tissues and 44.1% in the cancer tissues. In all normal tissues, β-catenin was expressed only at the cellular membrane but not in the cytosol or nucleus, while the expression of β-catenin was present in the cytosol or nucleus in 29.4% of the adjacent tissues and 50.0% of the cancer tissues. The positive rate of CDH1 gene promoter methylation was negatively correlated with E-cadherin expression(r=-0.312, P=0.01) and positively correlated with β-catenin cytosolic/nucleus expression(r=0.309, P=0.018). The differentiation and metastasis of colonic carcinoma were associated with the aberrant expression of E-cadherin, β-catenin, and methylation of CDH1 promoter (P<0.05). CDH1 gene promoter methylation may lead to aberrant expression of E-cadherin and β-catenin in colonic carcinoma, and may play an important role in promoting the invasion of tumor.

Expression of connexin 43 and E-cadherin in choroidal melanoma.

To investigate the expression of connexin 43 and epithelial cadherin (E-cadherin) in choroidal melanoma, to explore the clinical and pathological implications of expression of these proteins, and to determine their relations with malignant features. The expression of connexin 43 and E-cadherin in choroidal melanoma were detected by immunohistochemistry and correlated with clinicopathological features. Positive rates of connexin 43 in choroidal melanomas and benign pigmented nevus tissues were 75% and 40% respectively with significant differences between the two groups (χ(2)=5.607, P=0.009). Positive rates of E-cadherin in choroidal melanomas and benign pigmented nevus tissues were 40% and 75% respectively with significant differences between the two groups (χ(2)=5.214, P=0.010). Significant overexpression of connexin 43 and reduction of E-cadherin expression was associated with the invasion to the sclera, and there were respectively significant differences between without and with scleral invasion groups (χ(2)=2.880, P=0.040; χ(2)=2.778, P=0.046). Overexpression of connexin 43 were correlated with tumor cell types and the expression of connexin 43 and E-cadherin may be correlated with each other. The increased expression of connexin 43 and the decreased expression of E-cadherin may be involved in the process of invasion of choroidal melanoma. The overepression of connexin 43 and reduction of E-cadherin may contribute to the development of choroidal melanoma.

The expression detection of epithelial to mesenchymal transition related proteins and screening of its differential expression microRNAs in hepatocellular carcinoma

Objective To detect the expression o-f E-cadherin and vimentin, and their correspond- ing microRNAs （miRNAs） in primary heptocellular carcinoma （HCC）. Methods The expression of E-cadherin and vimentin in 32 cases of HCC tissues was examined by using immunohistochemistry, and the relationship between the expression and clinicopathology was analyzed, miRNA array was used to investigate the differentially expressed miRNAs between E-cadherin （ - ）/vimentin （ ＋ ） metastasis HCC and E-cadherin （ ＋ ）/vimentin （ - ） no-metastasis HCC. Real-time polymerase chain reaction （PCR） was applied to verify the reliability of miRNA array results. We predicted target genes of the four miRNAs by com- bination anticipated algorithms. Results The positive rate of E-cadherin in the metastasis, poor/no differentiation, 〉3 cm groups was 25.0% （2/8）, 35.7% （5/14）, 52. 9% （9/17） respectively, and that of vimentin was 87.5% （7/8）, 71.4% （ 10/14）, 52. 9% （ 9/17 ） respectively. The low expression of E-cadherin and high expression of vimentin in HCC was significantly correlated with metastasis and poor differentiation of HCC （P 〈 0.05 ）. miRNA array showed that 8 miRNAs were up-regulated and 28 miR- NAs were down-regulated in E-cadherin （ - ）/vimentin （ ＋ ） metastasis HCC. The expression levels of miR-21 and miR-221 in E-cadherin （ - ）/vimentin （ ＋ ） metastasis HCC were 2. 22 ± 1.79 and 2. 36± 1.05, significantly higher than those in E-cadherin （ ＋ ）/vimentin （ - ） no-metastasis HCC （4. 35 ±1.90, 3.90 ± 1.23,P 〈 0.05）. The expression levels of miR-126 and miR-199a in E-cadherin （ -）/ vimentin （ ＋ ） metastasis HCC were 4. 42 ±0.61 and 3.62 ±0. 54, notably lower than those in E-cadherin （ ＋）/vimentin （-） no-metastasis HCC （2.43 ±0.85, 2.54±1.10,P〈0.05）. Conclusion miRNA differential expression profile of E-eadherin （ - ）/vimentin （ ＋ ） HCC was obtained, and some of miRNAs may play a role in metastasis of HCC by regulating epithelial to mesenchymal transition. Key words: Careinoma, hepatoeellular; inicroRNA ; Metastasis ; Real-time PCR

The Potential Involvement of E-cadherin and β-catenins in Meningioma

ObjectiveTo investigate the potential involvements of E-cadherin and β-catenin in meningioma.MethodsImmunohistochemistry staining was performed on samples from patients with meningioma. The results were graded according to the positive ratio and intensity of tissue immunoreactivity. The expression of E-cadherin and β-catenin in meningioma was analyzed by its relationship with WHO2007 grading, invasion, peritumoral edema and postoperative recurrence.ResultsThe positive rates of E-cadherin in meningioma WHO I, II, III were 92.69%, 33.33% and 0, respectively, (P<0.05); while the positive rates of β-catenin in meningioma WHO I, II, III were 82.93%, 33.33% and 20.00%, respectively, (P<0.05). The positive rate of E-cadherin in meningioma without invasion (94.12%) was higher than that with invasion (46.67%) (P<0.05). The difference in the positive rate of β-catenin between meningioma without invasion (88.24%) and meningioma with invasion (33.33%, P<0.05) was also statically significant. The positive rates of E-cadherin in meningioma with peritumoral edema 0, 1, 2, 3 were 93.75%, 85.71%, 60.00% and 0 respectively, (P<0.05); the positive rates of β-catenin in meningioma with peritumoral edema 0, 1, 2, 3 were 87.50%, 85.71%, 30.00% and 0 respectively, (P<0.01). The positive rates of E- cadherin in meningioma with postoperative recurrence were 33.33%, and the positive rate with postoperative non-recurrence was 90.00% (P<0.01). The positive rates of β-catenin in meningioma with postoperative recurrence and non-recurrence were 11.11%, 85.00%, respectively (P<0.01).ConclusionThe expression levels of E- cadherin and β-catenin correlated closely to the WHO 2007 grading criteria for meningioma. In atypical or malignant meningioma, the expression levels of E-cadherin and β-catenin were significantly lower. The expression levels of E- cadherin and β-catenin were also closely correlated with the invasion status of meningioma, the size of the peritumoral edema and the recurrent probabilities of the meningioma, all in an inverse correlationship. Taken together, the present study provided novel molecular targets in clinical treatments to meningioma.

Expression and clinical significance of Ezrin and E-cadherin in esophageal squamous cell carcinoma

It has been proven that Ezrin protein may interact with E-cadherin protein and take part in metastasis of tumor cells. This study was to investigate the expressions of Ezrin and E-cadherin in esophageal squamous cell carcinoma (ESCC) and their relationship with the clinicopathologic factors, and analyze their diagnostic values for ESCC. The expression of Ezrin and E-cadherin in 72 specimen of ESCC and the paracancer normal squamous epithelium was detected using tissue array with SP immunohistochemistry. Their correlations to the clinicopathologic factors were analyzed statistically. The positive rate of Ezrin was significantly higher in ESCC than in para-cancer normal squamous epithelium (90.7% vs. 46.0%, P < 0.001); the positive rate of E-cadherin was significantly lower in ESCC than in para-cancer normal squamous epithelium (27.6% vs. 97.4%, P < 0.001). Ezrin expression was related to the invasiveness and lymph node metastasis of ESCC (P < 0.05); E-cadherin expression was related to the differentiation and lymph node metastasis of ESCC (P < 0.05). The high expression of Ezrin was related to the low expression of E-cadherin (P < 0.05). The activation of Ezrin and the absence of E-cadherin contribute to the tumorigenesis and metastasis of ESCC.

Can E-cadherin and CD34 be used as indicators of prognosis for hepatocellular carcinoma patients?

The high postoperative recurrence of hepatocellular carcinoma (HCC) is a problem that would benefit from the identification of indicators of recurrence and prognosis. In the past few years, research has shown that E-cadherin and CD34 can be used as indicators of the invasion of malignant tumors. In the present study, we investigated the expression of E-cadherin and CD34 in HCC patients. Expression levels of E-cadherin and CD34 in 41 HCC samples were detected using two-step immunohistochemical methods and compared with clinical pathological parameters and survival rate. The positive rates of E-cadherin and CD34 expression in 41 HCC cases were 48.78% and 100%, respectively. Expression of E-cadherin was significantly lower in patients with larger tumors, a high risk invasion and Edmondson classification III or IV (p<0.05). There was a significant relationship between CD34 expression and age and tumor invasiveness (p<0.05). There was no significant relationship between expression of CD34 and E-cadherin by Spearman statistical analysis (p>0.05). The survival rate in patients with negative expression of E-cadherin was significantly lower. The expression of CD34 cannot be used singly as a prognostic indicator for HCC patients. The co-expression of E-cadherin and CD34 cannot be used as a prognostic indicator for HCC patients. Clin Chem Lab Med 2008;46:1122-6.

Clinicopathological and biological features of micrometastasis in early gastric cancers

To investigate the clinicopathological and biological features of micrometastasis in early gastric cancers. Eleven cases of early gastric cancer with micrometastasis (micrometastatic, MM group) and 46 cases of early gastric cancer with lymph node metastasis (control group) were included in the study. Immunochemical staining of ssDNA, bcl-2, p53, E-cadherin, Ki-67, CD34 was performed. The superficial lesions, invasive fronts and lymph nodes were examined in both groups. Positive rate of ssDNA at the superficial lesions in MM group was higher than that in control group. In MM group the positive rate of ssDNA in micrometastasis was higher than that at invasive fronts and in lymph nodes. Positive rate of bcl-2 at the superficial lesions in micrometastasis was higher than that at invasive fronts and lymph nodes. Positive rate of c-myc at the superficial lesions in MM group was higher than that in control group. Positive rate of E-cadherin and the percentage of microvascular areas at the lymph nodes in MM group was lower than those in control group. Proliferative ability of cancer cells at superficial lesions and lymph nodes in MM group was lower than those in control group. Lymph nodes <3 mm in micrometastasis accounted for 27.3%. The pathological and biological features of micrometastasis in early gastric cancer show low positive rate of ssDNA, E-cadherin, Ki-67 and low percentage of microvascular areas at the lymph nodes.

Expression of survivin and E-cadherin in breast cancer

Objective: Survivin is a member of the inhibitor of apoptosis (IAP) family, and is involved in the regulation of cell division. E-cadherin functionally belongs to transmembrane glycoproteins family, it is responsible for intercellular junction mechanism that is crucial for the mutual association of vertebrate cells. These genes are thought to be associated with cancer aggression. This study was to investigate the relationship between surviving gene, E-cadherin expression and invasion clinicopathological features of breast cancer. Methods: The expression of surviving gene and E-cadherin were detected by SP immunohistochemical technique in tissues of 66 breast cancer, 20 breast fibroadenoma and 20 adjacent breast tissue. Results: The positive rate of surviving gene expression in breast cancer was 42.2%, significantly higher (P=0.025) than those in breast fibroadenoma (35.0%), and adjacent breast tissue (10.0%). The positive rate of E-cadherin in the groups of adjacent breast tissue, breast fibroadenoma and breast cancer were 100%, 100% and 42.4%, there was significant difference between the group of benign and malignant tumor (P=0.005). The positive rate of surviving in breast cancer with local lymph node metastasis was significant higher than that in breast cancer without lymph node metastasis (P=0.01), and E-cadherin in breast cancer with local lymph node metastasis was significant lower than that without lymph node metastasis (P=0.01). There was no significant difference among the groups of pathological types and TNM stages in the expression of surviving (P=0.966 & P=0.856), but there was significant difference in the expression of E-cadherin among these groups (P=0.01 & P=0.023). Conclusion: The loss or decrease of E-cadherin expression may promote the exfoliation of cancerous cells from original tissues, and surviving gene may promote the viability of the exfoliated cancer cells and the formation of new metastasis focus. These 2 factors cooperate with each other in the process of metastasis and invasion. They have a close relation with the breast cancer clinicopathological features.