Cell Wall Pores Research Articles

Characterization of water states and pore size distribution in Beijing poplar using nuclear magnetic resonance techniques

ABSTRACT This study employs nuclear magnetic resonance (NMR) techniques to elucidate the water states and pore size distribution within Beijing poplar (Populus beijingensis W. Y. Hsu) across various temperatures. By analyzing the variation in moisture content (MC) at different freezing temperatures, the proportion and distribution of cell wall pores were determined using the NMR Cryoporometry (NMR-C) method. The results show approximately 60% of the pores in both the heartwood (HW) and sapwood (SW) of Beijing poplar have diameters were 1.5 nm, while the proportion of pores with diameters greater than 4.6 nm was less than 10%. Moreover, the MC of both more freely bound water (C-water) and OH bound water (B-water) is observed to decrease exponentially with decreasing temperature. B-water in Beijing poplar is found to maintain a higher MC than C-water across the temperature range from −3 to −60°C, indicating greater resistance to freezing. The findings of this study contribute to a comprehensive understanding of water states in Beijing poplar. Also the pore size distribution of Beijing poplar provides reference data for the selection of wood chemical modification groups.

The role of cathelicidins in neutrophil biology.

Despite their relatively short lifespan, neutrophils are tasked with counteracting pathogens through various functions, including phagocytosis, production of reactive oxygen species (ROS), neutrophil extracellular traps (NETs), and host defence peptides. Regarding the latter, small cationic cathelicidins present a conundrum in neutrophil function. Although primarily recognized as microbicides with an ability to provoke pores in microbial cell walls, the ability of cathelicidin to modulate key neutrophil functions is also of great importance, including the release of chemo-attractants, cytokines and ROS, plus prolonging neutrophil lifespan. Cumulative evidence indicates a less recognized role of cathelicidin as an "immunomodulator;" however, this term is not always explicit and its relevance in neutrophil responses during infection and inflammation is seldom discussed. This review compiles and discusses studies of how neutrophils use cathelicidin to respond to infections, while also acknowledging immunomodulatory aspects of cathelicidin through potential crosstalk between sources of the peptide.

Sound absorption performance and mechanism of aluminum foam with double-layer structures of conventional and porous cell walls

Open-cell aluminum foam exhibits sound absorption valleys in the 2500-4000 Hz range, reducing its sound absorption performance in the 800–6300 Hz range. This paper prepared a novel aluminum foam with a double-layer structure using the infiltration casting method, and its pore structure, sound absorption performance, acoustic model, and sound absorption mechanism are investigated. The double-layer structure consists of a conventional pore (CP) layer with a single pore size of 250 μm or 600 μm and a porous cell wall (PCW) layer with double pore diameters of 250 μm and 600 μm. As the thickness ratio between the CP and PCW layers decreases, the acoustic absorption valley value of the double-layer structure increases when combined with a small-pore CP layer. In contrast, it decreases slightly when combined with a large-pores CP layer. The double-layer structure reaches a sound absorption valley value and average sound absorption coefficient of 0.912 and 0.902, respectively. The double-layer structure's sound absorption valley value is better than that of a homogeneous structure, improving sound absorption performance. A Lu modified model is established to characterize the sound absorption of the double-layer structure, which closely matches the experimental results. The modified model further analyzed the vital geometrical parameters affecting the absorption valleys. Acoustic impedance analysis shows the enhanced sound absorption valleys are due to: 1) Gradient acoustic impedance reduces sound reflections at the air-structure interfaces and increases secondary reflection at the interfaces in the structure; 2) The series–parallel configuration increased the resonance peak bandwidth, while the dual-sized resonance cavities regulated the resonance peak frequency.

VND Genes Redundantly Regulate Cell Wall Thickening during Parasitic Nematode Infection.

Plant parasitic root-knot nematodes are major agricultural pests worldwide, as they infect plant roots and cause substantial damages to crop plants. Root-knot nematodes induce specialized feeding cells known as giant cells (GCs) in the root vasculature, which serve as nutrient reservoirs for the infecting nematodes. Here, we show that the cell walls of GCs thicken to form pitted patterns that superficially resemble metaxylem cells. Interestingly, VASCULAR-RELATED NAC-DOMAIN1 (VND1) was found to be upregulated, while the xylem-type programmed cell death marker XYLEM CYSTEINE PEPTIDASE 1 was downregulated upon nematode infection. The vnd2 and vnd3 mutants showed reduced secondary cell wall pore size, while the vnd1 vnd2 vnd3 triple mutant produced significantly fewer nematode egg masses when compared with the wild type. These results suggest that the GC development pathway likely shares common signaling modules with the metaxylem differentiation pathway and VND1, VND2, and VND3 redundantly regulate plant-nematode interaction through secondary cell wall formation.

Study on sound absorption valley and acoustic absorption performance of small-pore aluminum foam composited with 304 stainless steel fibers

Open-cell aluminum foam exhibits a sound absorption valley (SAV) in the 2500–4000 Hz frequency range, reducing its acoustic absorption in the range of 1000–6300 Hz. A small-pore aluminum foam composited with 304 stainless steel fibers (SP-SS304F aluminum foam) with a porosity of 82 % was fabricated via infiltration casting, and its pore structure, acoustic absorption performance, and mechanism were investigated. The pore structure of SP-SS304F aluminum foam consisted of 223–676 μm main pores, 125–373 μm cell wall pores, and 30–60 μm 304 stainless steel fibers. The fibers existed completely embedded in the cell walls, partially or completely embedded in the cell cavities. As the pore diameter decreases, the SAV value increases and then decreases. With increasing fiber content, the SAV value showed a similar trend. The increased SAV value benefits the enhancement of the average sound absorption coefficient (SAC). The SP-SS304F foam aluminum reaches an optimal SAC value of 0.899. Its static resistivity value is 24,345 Pa.s/m2. Finite element simulations reveal the reasons for the improved acoustic absorption performance of SP-SS304F aluminum foam: First, the small-diameter and pore-embedded fibers improve the acoustic impedance matching, reducing sound reflections at the SAV frequency. Second, the reduced pore diameters and partially or completely pore-embedded fibers increase the pore surface areas, intensifying the acoustic absorption by pore structure.

Compression fatigue of elastomeric foams used in midsoles of running shoes

Due to their excellent specific mechanical properties, closed cell elastomeric foams are the main element in the soles of running shoes to absorb repetitive shocks from strides and to release a maximum of the absorbed energy. However, these cellular materials are gradually damaged. To enhance their mechanical durability by slowing their damage kinetics, it is critical to understand their mechanical behaviour in fatigue. The objective of this work is thus to clarify the link between the microstructure and the fatigue properties of five commercial elastomeric foams used in the midsoles of running shoes. The 3D cellular structures of each foam were finely analysed using X-ray microtomography. Foam samples were then subjected to cyclic compression which were close to running conditions. During cycling, samples exhibited a rapid densification associated with noticeable decreases of (i) the stress levels required to deform them as well as (ii) the cushioning and (iii) the rebound properties. We show that the two midsoles filled with micro-sized mineral fillers present the highest specific mechanical properties during the first compression cycle and during fatigue. However, their damage kinetics and rebound properties could probably be improved by tuning the fillers-matrix compatibility. The lightest foam, being very porous and presenting process-induced tortuous cell walls, is the best cushioning system but exhibits high damage kinetics. The densest foam presents poor specific mechanical properties, but very slow damage kinetics. Its double hierarchical architecture probably prevents the occurrence of micro-cracks in the cell walls.

Biomimetic Study of a Honeycomb Energy Absorption Structure Based on Straw Micro-Porous Structure.

In this paper, sorghum and reed, which possess light stem structures in nature, were selected as biomimetic prototypes. Based on their mechanical stability characteristics-the porous structure at the node feature and the porous feature in the outer skin- biomimetic optimization design, simulation, and experimental research on both the traditional hexagonal structure and a hexagonal honeycomb structure were carried out. According to the two types of straw microcell and chamber structure characteristics, as well as the cellular energy absorption structure for the bionic optimization design, 22 honeycomb structures in 6 categories were considered, including a corrugated cell wall bionic design, a modular cell design, a reinforcement plate structure, and a self-similar structure, as well as a porous cell wall structure and gradient structures of variable wall thickness. Among them, HTPC-3 (a combined honeycomb structure), HSHT (a self-similar honeycomb structure), and HBCT-257 (a radial gradient variable wall thickness honeycomb structure) had the best performance: their energy absorption was 41.06%, 17.84%, and 83.59% higher than that of HHT (the traditional hexagonal honeycomb decoupling unit), respectively. Compared with HHT (a traditional hexagon honeycomb decoupling unit), the specific energy absorption was increased by 39.98%, 17.24%, and 26.61%, respectively. Verification test analysis revealed that the combined honeycomb structure performed the best and that its specific energy absorption was 22.82% higher than that of the traditional hexagonal structure.

3D pore structure characterization and permeability anisotropy visualization simulation of fusain

The Jurassic‒Cretaceous coal seams in northern China are rich in fusains, and the pore size of the residual plant tissue in fusains is much larger than the limit value of seepage pores and even larger than that of some microfractures in coal. Therefore, understanding the 3D pore structure and permeability of fusains is helpful for determining the physical characteristics of inertinite-rich coal reservoirs. The porosity of the fusain varies widely, the pore structure has obvious directivity, and this study summarizes three pore types. Based on the visual distribution module of fluid migration, three types of seepage models of fusains were established, including the tubular cell structure seepage model, the broken cell wall pore seepage model, and the complete cell structure surface barrier seepage model. The results of the physical simulation verify the rationality of the three seepage models and show that the degree of fusification and cell pore fragmentation strongly affect the permeability of the fusain. For a low-rank coal reservoir with a rich fusain, the overall pore permeability is high, which is conducive to the seepage production of coalbed methane and is an important reference value for high-permeability layer selection.

Synergistic Effects of Heating Platens’ Temperature and Compression Ratio on the Periodic Hot-Press Drying of Chinese Fir Lumber

The effects of periodic hot-press drying on drying behavior and mechanical damage to Chinese fir lumber were investigated by taking the heating platens’ temperature (TP) and compression ratio (Rc) as experimental factors. The temperature and pressure inside lumber were analyzed during drying process. The results were as follows. The drying rate of lumber was significantly increased with increasing TP and Rc. Scanning electron microscope (SEM) micrographs showed that bordered pit membranes, cross-field pits, middle lamella between adjacent cells, and tracheid walls were damaged after drying, and the damage became more severe with higher TP and Rc. Detachments between ray parenchyma cells and tracheids were observed at 170 °C. Nitrogen-adsorption measurement results demonstrated that more cell wall pores in the 2.5~6.2 nm pore diameter range were generated at higher TP, resulting in an enlarged specific surface area and pore volume of cell walls. These structural changes contributed to accelerating moisture migration and decreasing the drying time. Furthermore, fluctuating pressure inside lumber was the main driving force leading to moisture migration and cell tissue damage in lumber during drying. The influence of TP on internal temperature (TM) and pressure (PM) was greater than Rc. With the increase in TP from 130 to 170 °C at the Rc of 10%, the maximum TM and PM were increased by 30.90% and 39.84%, respectively. However, TP should not be too high to prevent the formation of macro-cracks caused by high pressure, which may significantly affect wood’s mechanical properties. These results provide theoretical support for periodic hot-press drying processes’ improvement and high-value utilization of Chinese fir.

Influence of natural fibers on hydration and carbonation of reactive magnesium oxide cement (RMC)

This study offers insights into the influence of cellulose fibers at 0, 0.25, and 0.5 vol% on the hydration and carbonation of reactive magnesium oxide cement (RMC). The cumulative heat increased with the fiber content, attributed to the nucleation and possibly internal curing effects of the fibers. The reinforcing action provided by the fibers also improved the mechanical performance of the air-cured samples, yet the compressive strength under 20% CO2 and 80% RH was maximized at 0.25 vol%. At 0.5 vol%, the contents of brucite and carbonates were reduced due to confinement and crack control, which restricted the growth of brucite and CO2 diffusion. The carbonation phase was mainly poorly crystalline or amorphous, marked by a fused structure and with a thermal decomposition peak at approximately 405 °C. The cellulose fibers with a porous cell wall and cavity provided a pathway for enhanced CO2 diffusion, resulting in concentrated carbonates at the fiber-matrix interface.

Application of ZnO Nanoparticles Encapsulated in Mesoporous Silica on the Abaxial Side of a Solanum lycopersicum Leaf Enhances Zn Uptake and Translocation via the Phloem.

Foliar application of nutrient nanoparticles (NPs) is a promising strategy for improving fertilization efficiency in agriculture. Phloem translocation of NPs from leaves is required for efficient fertilization but is currently considered to be feasible only for NPs smaller than a cell wall pore size exclusion limit of <20 nm. Using mass spectrometry imaging, we provide here the first direct evidence for phloem localization and translocation of a larger (∼70 nm) fertilizer NP comprised of ZnO encapsulated in mesoporous SiO2 (ZnO@MSN) following foliar deposition. The Si content in the phloem tissue of the petiole connected to the dosed leaf was ∼10 times higher than in the xylem tissue, and ∼100 times higher than the phloem tissue of an untreated tomato plant petiole. Direct evidence of NPs in individual phloem cells has only previously been shown for smaller NPs introduced invasively in the plant. Furthermore, we show that uptake and translocation of the NPs can be enhanced by their application on the abaxial (lower) side of the leaf. Applying ZnO@MSN to the abaxial side of a single leaf resulted in a 56% higher uptake of Zn as well as higher translocation to the younger (upper) leaves and to the roots, than dosing the adaxial (top) side of a leaf. The higher abaxial uptake of NPs is in alignment with the higher stomatal density and lower density of mesophyll tissues on that side and has not been demonstrated before.

New insight into cell wall pore structure in brown-rotted wood and its utilization as a new low-cost, sustainable adsorbent

Brown-rotted wood is a substantial agroforestry waste produced by the economic fungal cultivation industry, which lacks further utilization. This study evaluated the degradation process and structural properties of brown-rotted wood (spruce and masson pine) obtained from different decay times (0, 4, 6, 10, and 14 weeks) from the perspective of pore structure. The adsorption performance and application potential of the brown-rotted wood for methylene blue (MB) were analyzed based on its structure and properties. The results showed that hemicellulose and cellulose were significantly degraded by brown-rot fungi, disrupting the compact structure of the cell wall and creating pores, especially in earlywood. In the initial stage (4 weeks), the degradation of the amorphous component increased the pore proportion of 5–10 nm size, which was conducive to enzymatic hydrolysis by improving the enzyme’s diffusion in the cell walls. The nitrogen adsorption analysis showed that the specific surface area and pore volume of the brown-rotted wood increased with decay processing. The surface areas of the brown-rotted wood were the largest at 14 weeks, which were 35.83 cm2/g (brown-rotted spruce) and 32.98 cm2/g (brown-rotted pine), respectively. The increase in the surface areas of the brown-rotted wood provided more space for adsorbing MB, of which, the adsorption capacity reached 48.22 mg/g and 44.58 mg/g, twice higher than the sound wood. The exploration of the interaction between cell wall matrix degradation and pore structure development provided new insight to elucidate the degradation mechanism of brown-rotted wood, and a vital basis for improving the adsorption efficiency of the brown-rotted wood as a new low-cost, sustainable adsorbent.

Optimization of delignification depth of wood surfaces for skin polymer impregnation

We controlled the degree of surface delignification of a wood specimen to impart functionality to its surface and injected a polymer into the porous structure to investigate using it as a functional material. Generally, balsa wood is used in studies aiming to impart functionality through polymer impregnation. However, we used a native Korean tree (Paulownia tomentosa), which has the lowest specific gravity. Peracetic acid (acetic acid and hydrogen peroxide mixture) was used as the delignification solution. Changes in wood surface properties were analyzed according to the delignification treatment duration. Changes in chemical composition, functional groups, cell wall structure, specific gravity, and pores were also observed. Depending on the delignification time, the specific gravity decreased from 0.24 to 0.12. The lignin content was reduced from 29.2% to 0.9%. Fourier Transform Infrared Spectroscopy revealed changes in the lignin functional groups (1 591, 1 507, and 1 240 cm−1), and changes in functional groups derived from hemicellulose (1 720 and 1 182 cm−1). Epoxy infiltration depth was adjusted according to delignification treatment depth to impart wood surface functionality. Our research results indicated that strength and durability could be improved by selectively impregnating polymers into the wood surface requiring functionality, unlike chemically modifying the entire interior of the wood.

Study on Modification of Poplar Wood via Composite Impregnation with Silica Sol/Melamine-Glyoxal Resin.

In order to overcome the defects of fast-growing poplar wood, such as low strength and poor toughness, this paper introduces a method of modifying poplar wood via impregnation with silica sol/melamine-glyoxal (silica sol/MG) resin and explores its effects on the physical, mechanical, and thermal properties of poplar wood. It was found via scanning electron microscopy that the composite modifier covered and filled the cell lumen, cell interstitial space, and cell wall pores of poplar wood. Further, infrared spectroscopy and X-ray photoelectron spectroscopy analyses confirmed that chemical cross-linking occurred between the silica sol/MG resin composite modifier and the internal groups of poplar wood and that the Si-O-Si flexible long chains introduced in the composite modifier formed a cross-linking network with poplar wood such as Si-O-Si and Si-O-C, which led to the improvement of the physical and mechanical properties and the enhancement of the thermal stability of poplar wood. The method provides a theoretical basis for the high-value utilization of fast-growing poplar wood.

Improving enzyme accessibility in the aqueous enzymatic extraction process by microwave-induced porous cell walls to increase oil body and protein yields

Aqueous enzymatic extraction (AEE) is a green and sustainable extraction method. However, the intact cell wall hinders the accessibility of enzymes, severely limiting the efficiency and application of the AEE method. In this study, microwave-assisted aqueous enzymatic extraction (MAAEE) was used to degrade the peanut cell wall and improve the yield of oil and protein. Transmission electron microscopy, scanning electron microscopy, BET-specific surface area, Fourier transform infrared spectroscopy, X-ray diffraction, and chemical composition analysis were used to characterize the structure and chemical changes of the peanut cell wall. The results showed that microwave treatment could break the structure of the peanut cell wall, increase the porosity of raw materials, accelerate the efficiency of enzymatic hydrolysis, and promote the extraction of oil and protein. Compared with raw peanut powder (MC-0), microwaved peanut powder (MC-4) had a higher oil body yield (>44%) and protein release rate (>76%). At the microscopic level, cell wall rupture, oil accumulation, and cell contents scattered outside the cell were observed. The mean pore size, pore volume, and specific surface area increased by 54.1%, 149.4%, and 63.5%, respectively, while the crystallinity decreased by 4.4%. The content of hemicellulose and CDTA-soluble pectin (CSP) decreased by 18.6% and 32.9%, respectively. The degradation rates of cellulase and pectinase on cell wall components were significantly increased by 8.5%–43.2% (p < 0.05). In summary, microwave-induced porous cell walls could significantly improve enzyme accessibility, accelerate enzymatic hydrolysis efficiency, and promote oil and protein extraction.

Facilitating enzymatic hydrolysis efficiency of rape straw by pretreatment with a novel glycerol-based deep eutectic solvent

Deep eutectic solvent (DES) pretreatment plays an indispensable role in destroying the natural anti-degradation barrier structure of lignocellulose. Here, the cetyltrimethylammonium bromide and glycerol were used to synthesize novel DES and introduced to the pretreatment process to facilitate the enzymatic hydrolysis efficiency of rape straw (RS). The results showed that the delignification and xylan removal could extremely reach 46.1% and 52.7% under optimal conditions (molar ratio of 1:6, 200 °C, 90 min), respectively. It also resulted in 171.4 mg/g higher accessibility, 7.2% higher crystallinity, and 122.3 m2/g lower lignin surface area in RS concerning morphological changes of deconstructed and porous cell wall structure. Ultimately, the reducing sugar yield of RS was increased from 27.4% to 67.9% after pretreatment with this type of DES. This study exploited a novel DES for facilely reducing the recalcitrance of lignocellulose and a promising process for efficient saccharification using renewable biomass.

Cesin, a short natural variant of nisin, displays potent antimicrobial activity against major pathogens despite lacking two C-terminal macrocycles.

Nisin is a widely used lantibiotic owing to its potent antimicrobial activity and its food-grade status. Its mode of action includes cell wall synthesis inhibition and pore formation, which are attributed to the lipid II binding and pore-forming domains, respectively. We discovered cesin, a short natural variant of nisin, produced by the psychrophilic anaerobe Clostridium estertheticum. Unlike other natural nisin variants, cesin lacks the two terminal macrocycles constituting the pore-forming domain. The current study aimed at heterologous expression and characterization of the antimicrobial activity and physicochemical properties of cesin. Following the successful heterologous expression of cesin in Lactococcus lactis, the lantibiotic demonstrated a broad and potent antimicrobial profile comparable to that of nisin. Determination of its mode of action using lipid II and lipoteichoic acid binding assays linked the potent antimicrobial activity to lipid II binding and electrostatic interactions with teichoic acids. Fluorescence microscopy showed that cesin lacks pore-forming ability in its natural form. Stability tests have shown the lantibiotic is highly stable at different pH values and temperature conditions, but that it can be degraded by trypsin. However, a bioengineered analog, cesin R15G, overcame the trypsin degradation, while keeping full antimicrobial activity. This study shows that cesin is a novel (small) nisin variant that efficiently kills target bacteria by inhibiting cell wall synthesis without pore formation. IMPORTANCE The current increase in antibiotic-resistant pathogens necessitates the discovery and application of novel antimicrobials. In this regard, we recently discovered cesin, which is a short natural variant of nisin produced by the psychrophilic Clostridium estertheticum. However, its suitability as an antimicrobial compound was in doubt due to its structural resemblance to nisin(1-22), a bioengineered short variant of nisin with low antimicrobial activity. Here, we show by heterologous expression, purification, and characterization that the potency of cesin is not only much higher than that of nisin(1-22), but that it is even comparable to the full-length nisin, despite lacking two C-terminal rings that are essential for nisin's activity. We show that cesin is a suitable scaffold for bioengineering to improve its applicability, such as resistance to trypsin. This study demonstrates the suitability of cesin for future application in food and/or for health as a potent and stable antimicrobial compound.

Hierarchical wood cells impose well-textured carbon nanotubes with cobalt single atoms: Bioinspired construction and application in zinc–air battery

The construction of oxygen reduction reaction (ORR) electrocatalysts with well-textured structure and desirable activity remains great challenge for the complicated procedures and poor interface. Herein, inspired by sea anemones, the highly ordered Co–based carbon nanotubes electrocatalysts were elaborately assembled on wood fibers via stoichiometry. The porous cell-wall and abundant oxygen groups in lignocellulosic scaffold impose such preferable structure, in which the Co–based carbon nanotubes with Co single atoms and Co nanoparticles were tuned to possess higher catalytic activity and capture more oxygen species. As expected, the as-prepared electrocatalyst displays outstanding ORR performance with a half-wave potential of 0.9 V. The assembled Zn–air battery using this cathode catalyst shows a high-power density of 196 mW cm−2, superior to the commercial Pt/C catalysts. This work could inspire developing other biomimetic architectures for high-performance energy storage and conversion systems.

Sound Absorption Performance and Mechanism of Aluminum Foams with Double Main Pore‐Porous Cell Wall Structure

To enhance the sound absorption performance of open‐cell aluminum foam, the double main pores‐porous cell walls (DMP‐PCW) aluminum foams via infiltration casting of preforms mixed with two sizes of NaCl particles are prepared. The pore structure, sound absorption performance, and mechanism of DMP‐PCW aluminum foam are investigated. The pore structure consists of double‐sized main pores similar to the NaCl particles and the cell wall pores formed by the connections between NaCl particles. It is found that the static flow resistivity of DMP‐PCW aluminum foam reaches an optimum value of 28105 Pa.s m−2 when the volume proportion of small main pores increases, the size of cell wall pores decreases, and the number of cell wall pores per unit main pore surface area (NPPA) increases. At 800–6300 Hz, the average absorption coefficient is 0.89. In addition, the Wilson model predicts the sound absorption properties of DMP‐PCW aluminum foam. The predicted values agree well with the measured values. The finite‐element acoustic simulations and dynamic viscous‐thermal permeability calculations reveal that the improved sound absorption performance of DMP‐PCW aluminum foam is correlated to the enhanced sound transmission caused by increased NPPA and increased viscous‐thermal loss due to the double main pore structure.

The influence of partial chemical component removal on water state at high relative humidity and cell wall saturation state

Water sorption in wood is mostly determined by the cell wall pores and the availability of sorption sites. In this study, we partially removed hemicellulose and lignin to adjust hydroxyl accessibility and pore size distribution of the cell wall of beech (Fagus sylvatica). With the assistance of the two-dimensional time-domain nuclear magnetic resonance technique, the water state at high relative humidity (RH) regions and cell wall saturation state were investigated. Both hemicellulose removal and delignification brought newly created pore spaces, lowered the average pore size, and increased the specific surface area. At the same time, a decrease in hydroxyl accessibility was observed for the hemicellulose-removal samples, but an increase in delignified samples. Two cell wall waters (B-water and C-water) with different mobility and two cell lumen waters were categorized. At high RH regions, more water molecules accumulated in microfibril and its surrounding hemicellulose (the domain of B-water) with lower mobility after partial chemical components removal. In combination with the mobility change with the physiochemical environment of the water molecules, it may be established that the accumulation of B-water was affected more by the change in the physical environment. Furthermore, cell wall saturation may alter the matrix environment for both treated and untreated samples; following water saturation, the mobility of B-water increased in the delignified samples but decreased in the untreated and partially hemicellulose-removed samples.