Abstract
Deep eutectic solvent (DES) pretreatment plays an indispensable role in destroying the natural anti-degradation barrier structure of lignocellulose. Here, the cetyltrimethylammonium bromide and glycerol were used to synthesize novel DES and introduced to the pretreatment process to facilitate the enzymatic hydrolysis efficiency of rape straw (RS). The results showed that the delignification and xylan removal could extremely reach 46.1% and 52.7% under optimal conditions (molar ratio of 1:6, 200 °C, 90 min), respectively. It also resulted in 171.4 mg/g higher accessibility, 7.2% higher crystallinity, and 122.3 m2/g lower lignin surface area in RS concerning morphological changes of deconstructed and porous cell wall structure. Ultimately, the reducing sugar yield of RS was increased from 27.4% to 67.9% after pretreatment with this type of DES. This study exploited a novel DES for facilely reducing the recalcitrance of lignocellulose and a promising process for efficient saccharification using renewable biomass.
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