Breast cancer is the 3rd most common cancer in the world and the most common one in women. An alternative pharmacological treatment for breast cancer is needed. Kaempferia pandurata is an endemic plant in Asia which is known for its biological activity, of which is an anticancer activity with its most abundant bioactive compound, pinostrobin. The rhizome of Kaempferia pandurata is dried and extracted using maseration with n-Hexane solvent. The extract then isolated using the recrystallization method in methanol solvent to produce pinostrobin crystal. Pinostrobin is manufactured into nanoparticle using chitosan and sodium alginate polymer, which then analyzed using TEM and UV-Vis test. The pinostrobin and its nanoparticle counterpart are tested in MTT assay to show its inhibitory activity and then analyzed. TEM and UV-Vis test to the nanoparticle of pinostrobin showed the nanoparticle's dimension of <200 nm with a yield of 99.43%. MTT assay showed good anticancer activity from both pinostrobin and its nanoparticle. Better activity is shown by MDAMB-231 cell, while nanoparticle of pinostrobin showed better activity. The nanoparticle of pinostrobin has been manufactured with a decent yield and dimension. Better anticancer activity is shown in pinostrobin nanoparticle while the anticancer activity of MDAMB-231 cell is superior to MCF-7 cell for both samples. Pinostrobin can be considered as a potential drug for breast cancer.