Polyherbal Extract Research Articles

Identification of phytobioconstituents present in Simmondsia chinensis L. seeds extract by GC-MS analysis

Simmondsia chinensis L. commonly called as Jojoba and belongs to family Simmondsiaceae. It has shown positive pharmacological activities of these compounds which include antidiabetic, antirheumatic, anthelminthic, antipsoriatic, antioxidant, antiepileptic, antigonorrheal, analgesic, anti-inflammatory, and pesticidal activity of jojoba. The multifaceted action of numerous bioactives existing in the seed extract with therapeutic activity have attracted great research interest by pharmaceutical industries. n-hexane extract of Simmondsia chinensis L. (SC) Seeds was analysed by gas chromatography-mass spectroscopy for identification and characterization of phytobioconstituents and its therapeutic claim by traditional system. The major compounds discovered in SC seeds extract are cis-9-octadecen-1-ol (24.85%), 9-octadecen-1-ol, (Z)- (18.24%), Stigmast-5-en-3-ol (14.10%), Ergost-5-en-3-ol, (3-β)-ol (5.26%), (Z)-14-tricosenyl formate (5.24%), Thiositosteroldisulfide (3.64%), Silane, Dimethyl (dimethylpentyloxysilyloxy) tetradecyloxy- (3.41%), Ergost-5-ene, 3-methoxy-, (3β,24R)- (2.55%), Ergosta-5,22-dien-3-ol (2.22%), 1,19-eicosadiene (2.17%), Pentacosane (2.02%), Stigmasta-5,22-dien-3-ol (1.64%), 1,19-eicosadiene (1.57%), 9-octadecen-1-ol, (Z)- (1.46%), 9,19-cyclo-9β-lanostan-3β-ol, 24-methylene- (1.14%), (9Z)-9-octadecenyl palmitate (1.50%), Hexadecanoic acid, 9-octadecenyl ester, (Z) (1.37%), 9Z)-9-octadecenyl (9Z)-9-hexadecenoate (1.01%). The hexane extract of Simmondsia chinensis seeds comprises various polar and nonpolar phytobioconstituents. These compounds were established qualitatively via GC-MS evaluation. GC-MS reports will be promising in pharmaceutical sector in identification of variety of Phytobioconstituents in distinct plant extracts, polyherbal extract and the standardization of particular plant materials.

Formulation of Polyherbal Carbonated Beverage Based on Halalan Thoyyiban Principle

Herbal products usually have less palatability on their taste even though they are healthy and nutritious. Thus, this study aims to formulate polyherbal carbonated beverages, followed by the evaluation of sensory, physicochemical properties and their compliances with the halalan thoyyiban principle. Five formulations were prepared by varying the amount of water and polyherbal extracts that were designated using Design Expert 6.0.4 software. The most accepted formulation was formulation 1 (F1), containing 12.50% polyherbal extract and 87.50% water. F1 was characterised for its physicochemical properties, including carbon dioxide volume, pH, and total acidity, with 3.0 g/L, 4.5, and 1.7 %, respectively, and these findings have complied with the halalan thoyyiban principle. The antioxidant properties were evaluated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant potential (FRAP), as well as total phenolic content (TPC), and it exhibited 1.25 mg GAE/mL,1.08 mM Fe (II) and 0.39 mg GAE/mL respectively. Eight Halal Control Point (HCP) were identified along with the production. Moreover, the beverage was found to have antioxidant properties and nutritional content (carbohydrate (0.3%) and energy content (2.0 kcal/100g)), which can give health benefits to the consumer and proven safe for consumption (LC50 =1066.60 μg/ mL). To conclude, the formulated polyherbal carbonated beverage is accepted by the consumer and complies with the halalan thoyyiban principle.

Nephroprotective effects of polyherbal extract via attenuation of the severity of kidney dysfunction and oxidative damage in the diabetic experimental model.

In view of many complications of diabetes, kidney failure is considered as one of the main complications. The oxidative stress-induced due to persistent hyperglycemic conditions is the major cause of kidney disease. The present study was designed to explore the nephroprotective efficacy of polyherbal (PH) extract in a diabetic model induced by streptozotocin (STZ). STZ (55 mg/kg body weight, intraperitoneal) was injected in overnight fasting rats to develop the diabetic experimental model. Effect on kidney injury was evaluated by investigating biochemical and histological evidences in renal tissue after 56 days of treatment of PH extract. Results showed the high glucose level in STZ treated rats that suggested hyperglycemia persistence along with the successful establishment of nephropathy in diabetic rats with altered renal function, inflammatory cytokines level as well as oxidative and nitrosative stress. Administration of PH extract significantly improved the glycemic condition, glomerular function and proximal reabsorptive markers. Further, elevated pro-inflammatory cytokines levels and disturbed redox status were restored. Moreover, findings were fostered and substantiated by histopathological examinations. Our work strongly proposes that the nephroprotective effect of the PH extract on renal damage could be attributed due to its anti-inflammatory and antioxidant properties. Thus, PH extract could have potential as a pharmaceutical drug for diabetes mellitus (DM). Additional long-term study or clinical trial is required for further investigations.

A Clinical Study toEvaluate the Therapeutic Effect of Drug Tagaradi Kwath in the Management ofAnidra(Insomnia) –A Research Article

Anidra(Insomnia) is a type of sleep disorder in which individual feels difficulty in falling asleep, staying asleep or both. In Ayurveda, Anidrais indicated as VataNanatmajaVikaraby Acharya Charaka. Insomnia affects most of the people at any stage of life due to today’s stressful day to day life. Allopathic hypnotic drugs are used to treat insomnia in acute stage, but long-term use can lead to dependency and withdrawal symptoms along with other sideeffects. In present study a polyherbal compound Tagaradi kwathconsist extracts of Tagara, Turagagandha, Parpati, Shankhpushpi, Tridasavitapi, Tikta, Bharti (brahmi), Bhutakesi, Jaladhara, Krtamala, Cetaki and Gostanais used to check its efficacy in the management of insomnia. Aim: A clinical study to evaluate the therapeutic effect of drug Tagaradi kwathin the management of Anidra(Insomnia). Materials and methods: Total 30 newly diagnosed patients of age group 15 to 70 years of insomnia who were not taking any other hypnotic medicine were selected and administered 50ml ofKwathat bedtime for 1 month. Assessment was done considering the overall improvement of sleep quality according to Athens Insomnia Rating Scale, improvement in associated complains, relieving of anxiety and depression. Observations: The information gathered on the basis of observations was subjected to statistical analysis. The Paired ‘t’ test was used to check the significance of objective criteria. Results:The results showed that the trial drug had Significant effect on almost all the mentioned parameters of Insomnia.

Evaluations of anti-urolithiatic effect of Poly-herbal hydroalcohalic extract using calcium oxalate crystals precipitation method

Evaluations of anti-urolithiatic effect of Poly-herbal hydroalcohalic extract using calcium oxalate crystals precipitation method

Protective Effect of Polyherbal Extract on Gentamicin-Induced Renal Injury in Wistar Rats

Protective Effect of Polyherbal Extract on Gentamicin-Induced Renal Injury in Wistar Rats

Anti-Obesity and Anti-Diabetic Effects of a Polyherbal Extract Consisting of Coptidis Rhizoma, Salviae Miltiorrhizae Radix, and Cinnamomi Cortex in High Fat Diet-Induced Obesity Mice

Anti-Obesity and Anti-Diabetic Effects of a Polyherbal Extract Consisting of <i>Coptidis Rhizoma, Salviae Miltiorrhizae Radix</i>, and <i>Cinnamomi Cortex</i> in High Fat Diet-Induced Obesity Mice

Non-enzymatic Ex-vitro Antioxidant, Biosafety, Analgesic and Anti-inflammatory Evaluation of Aqueous Polyherbal (ELNA) Extract in Mice.

The polyherbal formulation known as ELNA consists of Moringa oleifera, Crateva religiosa and Curcuma longa. It ethnomedicinal properties include; analgesic, inflammation, cardiac diseases, bacterial infection and immune suppressed diseases. The aim of this study was to evaluate the ex-vitro non-enzymatic anti-oxidant, biosafety, analgesic and anti-inflammatory effect of aqueous ELNA polyherbal extract. Ex-vitro anti-oxidant study was investigated in ELNA extract using standard radical scavenging protocol. Biosafety effect of ELNA extract was understudied with the aid of Locke method. Acetic acid-induced peripheral pain, hot plate-induced central pain and egg albumin-induced inflammatory models were investigated. Results from this study exhibited the scavenging effect of ELNA extract against 1, 1-diphenyl-2-picryhydroxyl with significant increase in concentration dependent manner. Acute toxicity study of the aqueous extract of ELNA elicited less or no toxic effect, with no mortality and side effect above LD50&gt;5000 mg/kg body weight. Aqueous ELNA extract displayed no damaging effect of the sub-chronic toxicity study administered for 28 days, exhibited no significant difference (p= 0.05) in the organ and body weight. No significant different in the haematological parameters excluding 400 mg/kg with slight significant (p&lt;0.05) increase in platelet count. Graded doses of the extract at 400, 800 and 1200 mg/kg body weight exhibited significant (p&lt;0.05) reduction in central and peripheral pain. Significant reduction in paw edema volume. Specifically at higher doses, the extract showed an effective activities against the selected study. In conclusion, this study validated the ethnomedicianl evidence of ELNA aqueous extract of the formulation.

In-vitro Antioxidant activity, Biosafety, Nociceptive and Anti-inflammatory potential of Acetone Polyherbal (ELNA) Extract in mice

In-vitro Antioxidant activity, Biosafety, Nociceptive and Anti-inflammatory potential of Acetone Polyherbal (ELNA) Extract in mice

Preclinical evaluation from in-silico to in-vivo study of polyherbal microsponge sunscreen gel.

Electromagnetic spectrum of the UV region predominantly becomes the reason for skin's detrimental effects that give the genesis of innumerable skin ailments; because of this reason, the sunscreen products are required before condition in day to day lifestyle; products such as moisturizers, lotions, creams, shampoos, and other hair and skin preparations are accessible and accompanied by sunscreen properties, but they do not provide extended effect, also causes side effects due to harsh chemicals. The present study focuses on the effects of polyherbal extracts containing Microsponge gel for the protection of skin from ultraviolet rays. In the present research, already prepared Microsponge gel through quasi-emulsion solvent diffusion (QESD) technique was used for the HPLC, in-silico, in-vitro antioxidant activity, and in-vivo study. AdmetSAR software tool was utilized for the in-silico study, whereas for the in-vivo study, UV radiations are given on Albino rats using solarimeter. Results shown the active constituents are non-carcinogenic and non-toxic; IC50 values show good antioxidant activity and minimal effect of UV radiations after application of the gel formulation on animal skin. The results manifest prominent effects on animal skin further test for presence of ascorbic acid level and total protein in blood further verify the efficacy of the formulation. The study consequently established a strong ground for further extensive clinical studies.

Development and Pre-Clinical Evaluation of Polyherbal Hand Wash

Hand-washing is significant in agriculture, housekeeping, and in addition vital in houses and daycare plans. The artificial handwash formulations an effort antiquated entice formulate a polyherbal hand wash victimization extract of Sida cardifolia, Azadirachta indica, aloe vera gel, and lemon juice. The pH of hand wash was ranging from 6.36 to 6.88. The handwash formulations were ranges from 56 cp to 62 cp. The formulation planned out along with lemon juice displayed the best antimicrobial utilization than the formulation while not lemon juice. The stableness reports no change in pH &amp; Viscosity referred to in the formulations. The effects of the present work support the internalization as well as utilization of herbs in the formulation to help give better results.

Improving Gallic Acid and Quercetin Bioavailability by Polymeric Nanoparticle Formulation

: The anticancer activity and pharmacokinetic properties of encapsulated polyherbal nanoparticles (Gallic acid and quercetin Nanocomposite) and polyherbal extract (Amla and pomegranate fruit peels) in normal and DMH-induced colorectal cancer in rats was examined in this work. In normal and DMH-induced rats, a pharmacokinetic study demonstrated that polyherbal nanoparticles had a typical sustained release profile with a 4-fold increase in bioavailability when compared to polyherbal extract. Based on serum–concentration profiles of polyherbal nanoparticles and polyherbal extract following oral administration, the pharmacokinetic parameters for polyherbal nanoparticles and polyherbal extract were established using a single compartmental approach. This research suggests that encapsulating Gallic acid and quercetin in polymeric nanoparticles improves their oral bioavailability and anti-colon cancer efficacy. Polymeric nanoparticles could be a novel therapeutic possibility for carcinogenesis prevention.

Analytical characterization of biomarkers in an optimized novel antidiabetic polyherbal formulation using high-performance thin-layer chromatography and liquid chromatography with tandem mass spectrometry

Background Diabetes mellitus is a chronic health issue that requires novel approaches to treatment and a multimodal approach to prevention. In the treatment of diabetes, a polyherbal formulation is the finest alternative medicine. A polyherbal formulation was developed in-house and evaluated for its antidiabetic potential on streptozotocin-induced diabetes rat. The same extract was now characterized analytically utilizing a variety of methods. Objective The goal of this study was to quantify the biomarkers in a novel antidiabetic polyherbal formulation made in-house with Cinnamonum zeylanicium bark, Eugenia jambolana seeds, Vinca rosea whole plant, and Gymnema sylvestre (GS) leaves, using high-performance thin-layer chromatography (HPTLC) and liquid chromatography with tandem mass spectrometry (LC–MS/MS). Materials and methods Cinnamaldehyde (CIN), gallic acid (GLA), vincristine (VC), vinblastine (VB), and gymnemic acid (GYA) were identified as bioactive components of polyherbal formulation hydroalcoholic extract utilizing HPTLC and LC–MS/MS. Acetonitrile, methanol, and 0.1 percent formic acid was used as mobile phase, chromatographic separation was accomplished in 30 min using a gradient system and a SUNFIRE C18, 250×4.6, 5-µm analytical column with a flow rate of 1.0 ml/min in LC–MS/MS research. Scanned in a positive mode with a scan speed of 100–2000 AMU/s over a mass range of 20–1974 Da. The electron-spray ionization mode was used, with a source temperature of 150°C and a desolvation temperature of 350°C. The HPTLC separation was performed using ethyl acetate/acetonitrile/water/formic acid/N-dimethyl formamide 5.5 : 2.5 : 0.5 : 1.0 : 0.5 (v/v) as the mobile phase on precoated silica gel 60 GF254 plates. At room temperature, the plates were developed to a distance of 9.0 cm. CIN, GLA, VC, VB, and GYA plates were scanned and measured at wavelengths of maximum absorption of 259, 287, 342, 355, and 387 nm, respectively. Band size, chamber-saturation duration, migration of the solvent front, slit width, and other experimental parameters were carefully examined, and the optimized chromatographic conditions were chosen. Results LC–MS analysis of the hydroalcoholic extract of the polyherbal formulation revealed the presence of all the five bioactive chemical constituents, CIN, GLA, VC, VB, and GYA. Similarly, the drug samples were satisfactorily resolved with Rf 1.81±0.01, 0.05±0.01, 0.02±0.01, 0.09±0.01, and 0.04±0.01 for CIN, GLA, VC, VB, and GYA respectively, using HPTLC. Conclusion The importance of combining Ayurvedic formulations with contemporary high-throughput screening techniques will spark new interest in more powerful biocompatible drug leads. The findings of this study lend scientific credence to the therapeutic applications of the polyherbal formulation.

Anti-Arthritic Evaluation of Ginger, Colchicum and Detoxified Nux-Vomica combination for Poly Herbal Unani Formulation

Introduction: Arthritis (Wajaul Mafasil) is a condition which is growing worldwide due to lifestyle, environmental and genetic factors. In Unani literature, there are many herbs which are praised for treatment of Arthritis. So polyherbal formulation contains Ginger, Colchicum and Nux- Vomica is taken in combination for arthritis study. This combination is not previously reported but used by unani practitioners. Method: Three crude herbs (Ginger, Colchicum and Nux vomica) were extracted out in both aqueous and hydro-alcoholic solvent. LD-50 of all extracts (aqueous and hydro-alcoholic extract) was determined. Now respective extracts were mixed in effective dose ratio to obtain aqueous and hydro-alcoholic dosage form. Finally both effective combinations (aqueous and hydro-alcoholic) convert into tablet dosage form to determine its anti-arthritic activity by Carrageenan Induced Oedema Test, Cotton Pellet Induced Granuloma Test and Freund’s adjuvant Induced Arthritis Test. The efficacy of the Unani formulation was compared with reference drug (Diclofenac sodium). Result and Discussion: in Carrageenan Oedema Test, animals in high dose hydro-alcoholic, shows decrease in paw volume significantly after 3 hours of Carrageenan injection. In Cotton Pellet Induced Granuloma Test, animals in high dose hydro-alcoholic, shows reduction in granuloma formation significantly. In Freund’s Adjuvant Induced Arthritis Test, The significant reduction in paw volume was found in high dose hydro-alcoholic. Conclusion: Conclusively this study establishes anti-arthritic potential of polyherbal extract in unani literature. Thus these drugs possess synergistic anti-arthritic potential in combination against acute, sub-acute as well as chronic arthritis. So in future compatible dosages form may be prepared for treating arthritis.

Biochemical and histological investigation on the protective effect of poly-herbal extract in high salt diet-fed male Wistar rats

BackgroundThrough different mechanisms, excessive intake of dietary salt has been documented as a risk factor for the development of elevated blood pressure and organ dysfunction. Therefore, this study evaluated the efficacy of combined extracts of four medicinal plants against high salt diet-induced succinate dehydrogenase depletion and alteration in vital organ (kidney, liver, and cardiac) hemostasis. MethodsThirty-five male Wistar rats with an average weight of 130 g were divided into 5 groups of 7 animals each. Group 1 and 2 rats were fed with normal rat chow (NRC) and 16% high salt diet (HSD) only respectively. Rats in groups 3, 4, and 5 were fed with 16% HSD and 800, 400, and 200 mg/kg poly-herbal extract (PHE), respectively, once daily for 4 weeks. The concentration and activities of creatinine, urea, sodium, magnesium, chloride, potassium, albumin, globulin, total protein, and alkaline phosphate (ALP) were estimated in the serum. Aspartate and alanine aminotransferases (AST and ALT), lactate dehydrogenase (LDH), succinate dehydrogenase (SDH) were estimated in the serum, liver, and aorta. The result revealed that the combined extract significantly (p < 0.05) lowered HSD-induced elevated serum concentration of kidney function markers and electrolytes. More so, a significant (p < 0.05) reduction in serum and tissue activities of liver and cardiac function markers was demonstrated by the poly-herbal extract. No significant changes were observed in serum albumin and globulin level, however total protein and tissue activities of SDH were significantly (p < 0.05) elevated by the PHE. The histopathological examination showed to a large extent, the extract conferred significant protection on the cytoarchitecture of the kidney, liver, and aorta. ConclusionConclusively, combined plants extract protected against high salt diet-induced biochemical and histological derangement in male Wistar rats.

In Vivo Antidiabetic Activities of Polyherbal Extracts against Streptozotocin- Nicotinamide Induced Type 2 Diabetic Mice Model

Diabetes mellitus is a chronic metabolic disorder and rapidly increasing cases of diabetes throughout the world are major concerns in both developed and developing countries. Currently available oral antidiabetic medicines have limitations in efficacy and safety, which in conjunction with the emergence of diabetes mellitus as a global epidemic have aided the popularity of alternative therapies to manage the disease safely and more efficiently. Herbal medicines are accepted as alternative therapies for blood sugar control. Gymnema sylvestre, Boerhavia diffusa, Tinospora cordifolia, and Argyreia nervosa are some potent herbs used for the management of diabetes. Mixing of these plants in different ratios may produce synergistic antidiabetic actions which may have greater antidiabetic activity. Hence, the present study aimed to determine the anti-diabetic activities of polyherbal extracts in streptozotocin-nicotinamide induced type 2 diabetic mice. The administration of polyherbal extract orally at doses of 100, 200 and 400 mg/kg significantly decreased the blood glucose levels compared to the control diabetic mice. The polyherbal extract significantly lowered the elevated total cholesterol, triglycerides and low-density lipoprotein levels, while increased the high-density lipoprotein indicating antihyperlipidemic activity. The present study reveals that polyherbal extract at a dose of 400 mg/kg body weight resulted in a significant decline (p &lt; 0.001) in blood glucose level.&#x0D; Keywords: Polyherbal extracts; diabetes mellitus; Oral glucose tolerance test; Streptozotocin-Nicotinamide

Formulation and Evaluation of Poly Herbal Liqui-Solid Compact for its Anti-Inflammatory Effect

Liquisolid approach is a potential method due to the ease in the production process, low cost, and manufacturing process because of their strong flow and compaction properties. The study's main goal is to create a liquisolid powder formulation from the polyherbal extract and evaluate its anti-inflammatory activity. Hibiscus cannabinus, Murraya koenigii, and Tabernaemontana divaricate are used to formulating polyherbal Liquisolid powder formulation to assess their anti-inflammatory action. The formulation is tested for its stability studies and anti-inflammatory activity, both in-vitro and in-vivo. The polyherbal liquisolid powder formulation (F5) has a minimum strength of 1kg/cm 2, while F2 &amp; F6 strength was 4 kg/cm2; FTIR studies showed no interactions between drug and excipients. To confirm the liquisolid powder formulation, further evaluations on friability, hardness, disintegration time, dissolution rate, and Differential scanning calorimetry and X-ray powder diffraction analysis were determined. The polyherbal extract's anti-inflammatory activities were evaluated using the paw edema and cotton pellet method, and the results are found to exhibit its inhibitory activity. The polyherbal extract of Liquisolid powder formulation was evaluated, and it showed a promising inhibitory activity to overcome the inflammation in the test species. Hence it can be used as an alternative therapy for curing inflammation.

Antimicrobial screening of polyherbal formulations traditionally used against gastrointestinal diseases.

Emerging antibiotic resistance has become a cosmopolitan problem and evoking researchers to search for new antimicrobials from natural constituents. The present study was intended to test the antimicrobial potential of traditionally used unexplored polyherbal recipes for curing digestive ailments. A total of 25 plants species were combined in different ratios to form 14 polyherbal recipes. After collecting and grinding plant parts, methanolic extracts of 14 polyherbal recipes were prepared by the cold maceration process. Antibacterial and antifungal activity of the polyherbal extracts was checked by agar well diffusion method at a concentration of 50 mg/ ml while minimum inhibitory concentration (MIC) was determined by serial dilution method. Polyherbal recipes B and D showed significant inhibition zone each against Vibrio cholerae (25.63; p < 0.001). Recipe G (23.33; p < 0.001) showed better efficacy against Escherichia coli. Recipe E and G significantly inhibited Proteus species (28.33; 24.33; p < 0.001). Recipe B was highly effective against Salmonella typhi. Recipe C, A and F had significant antifungal affect and inhibited Aspergillus nigar (28.67; p < 0.05), Aspergillus fumigatus (27; p < 0.01) Trichoderma (30; p < 0.001), Rhizopus (19.67; p < 0.01), and Fusarium graminearum (28.67;p < 0.001). Polyherbal formulations A, B, D, K, and N were active with the lowest concentration. MIC ranges within 3.12–25 mg/ml while minimum bactericidal concentration (MBC) between 12.5 and 50 mg/ml. Polyherbal recipes’ A, B, D, G, K and N have enhanced antimicrobial potential with better efficacy than tested antibiotics and should be evaluated for further scientific validation.

Biogenic silver nanoparticles of antibacterial activities for poly-herbal extracts in novel medicine

We are developed in the green activity of silver nitrate nanoparticles and are used materials of Mentha (M), Azadirachta indica (A) leaves, and have been implemented as the agent for stabilizing the poly-leaf extracts (LE) / pig liver acetone powder (PL) with complexes. An optimal extract absorption of LE/PL has been used in 500 mg then 200 mg individually, by attraction about 0.841%. Since the PL satisfied with an energetic mediator of the MA leaf extracts are 92.1667 ± 0.3626%. The antibacterial movement on made silver nanoparticles is Escherichia coli, then Staphylococcus aureus. These samples are calculated to additional the antimicrobial movement counter to verified in a pathogenic way in a gram of positive, negative with microbial and fungiform straining. It was made with fast production to Natural Garden Pest Control (non-toxic method) for the mixture of silver nanoparticles are showed hopeful to the movement of all the established microbial straining. The trend is improved through an augmented quantity echelon.

Screening of secondary metabolites and immunostimulation potential of poly herbal formoulation (Asparagus racemosus, Mucuna pruriens, Anacyclus pyrethrum and Tribulus terristeris) in Wistar Albino rats

Immune system is a defensive mechanism system which is responsible to defend against several foreign invading agents that harm individual health. It generates several types of cells which have ability to recognize, eliminate and kill all the undesirable agents. The immune system works by inhibition, multiplication, amplification, expression and induction response at different phases of immune system. Hence immunology is the most advance and recent biomedical research system which covers the prevention and treatment of wide range of medical disorders. In this study, Immunomodulatory activity of Polyherbal formulation was determined. First of all, plants methanolic extracts of Asparagus racemosus, Mucuna pruriens, Anacyclus pyrethrum, Tribulus terrestris were prepared. The methanolic extracts of each plant were taken separately in equal amount and mixed to prepare the Polyherbal formulation (PHF). Screening test was applied on PHF for quantitative analysis. Hemolytic activity, thrombolytic activity and antioxidant activity were determined on experimental animals. To demonstrate the effect of standard Immunol drug, CCl4 and polyherbal formulation extract on hematological parameters. The methanolic extract of PHF was dissolved in distilled water and the dose was selected at 100, 200 and 300 mg/kg body weight. Blood sample collected in EDTA as anticoagulant were processed to determine the effect of PHP on selected hematological parameters including hemoglobin (Hb), red blood cell counts (RBCs), white blood cells (WBCs) count and Platelets count. Liver enzymes like transaminases (ALT and AST), uric acid, urea and creatinine were determined by automated photometric method to evaluate the drug safety. Serum samples were using for the estimation of Immunoglobulin M and Immunoglobulin G using enzyme linked immunosorbent assay (ELISA). Further, the C-reactive protein (CRP) was also estimated using standard protocol. Phytochemical screening of methanolic extract of polyherbal formulation showed the presence of alkaloids, tannins, flavonoids, phenols and cardiac glycosides. Bioactive constituent’s alkaloids, flavonoids and phenols were present in higher concentration. The maximum hemolysis was seen at a dose of 800μg/ml (40 ± 0.38). The methanolic extracts of PHF were investigated for its thrombolytic activity. Addition of 800μl of streptokinase as positive control (30,000 IU/ ml) to clots and successive incubation for 90 minutes at 37 oC showed 74.53±1.11% lysis of clot. Distilled water was considered as negative control which exhibit insignificant percentage of lysis of clot (3.74±0.51). In the study, the methanolic extract of PHF showed highest thrombolytic activity at 800μg/ml concentrations (41±0.38) when compared with streptokinase (positive control). When all the groups were compared the serum level ALT, AST, TAS, TOS and OSI 50 index, a statistically significant difference was observed between the groups. A variety of antioxidant function approaches have been suggested. During the study, it was found that induction of CCl4 increases the number of altered macrophages. However, the administration of Polyherbal extract in CCl4 intoxicated animals, the number of altered macrophages came to level. Administration of polyherbal formulation extract in control mice also showed significant reduction of changed macrophages. Immunomodulatory drugs are substances that can alter the immune function of an individual, are called immunostimulants if their immune responses are enhanced, or are called immunosuppressant’s if they decrease. These medications are most widely used in infections, allergies and cancer infections. Modern medical treatment is also far-reaching because of the economic limitations in developed countries including Pakistan. For immunomodular operations, just a few plants have been tested. The analysis indicates that many medicinal plants and marine products have immunomodulatory function, however there is little proof that their use in clinical practice is not allowed. In future work in herbal medicine, therefore, immunomodulatory agents should become more relevant.