Different polyether urethanes (PUPEG200, PUPEG400, PUPEG600, PUPEG1000, PUPEG4000) were synthesized from five different polyethylene glycols (PEG 200, PEG 400, PEG 600, PEG 1000, PEG 4000). The polymers were characterized by Fourier transform infrared (FTIR) and X-ray diffraction (XRD) and their mechanical properties were evaluated. Polyether urethanes synthesized from higher-molecular-weight PEG (PEG 4000) show comparatively maximum hydrophilicity, minimum tensile strength, and maximum elongation at break (%). Hydrolytic degradation of synthesized polyether urethanes was studied in alkali solutions of different concentrations (0.5, 1, 3% NaOH), different mineral acids (5 and 10% HCl, H2SO4, HNO3), phosphate buffer saline (PBS), and saline water (10% NaCl solution). Swelling behavior of the polyether urethanes was measured in water and in PBS solution. In order to assess the cellular responses of these polyether urethanes, cytotoxicity analysis was carried out against the human skin cell line.